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5'-flanking Region Responsible for Endospermspecific Expression of Rice Prolamin Chimeric Gene in Transgenic Tobacco

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Science China Chemistry 1993年第11期36卷 1307-1314,1409页

作者：周先锦范云六 Laboratory of molecular biology Biotechnology Research Centre Chinese Academy of Agricultural Sciences Beijing 100081 PRC

The 5' upstream region (—680—+40) containing the complete signal peptide coding sequence of the rice seed storage prolamin gene was amplified in vitro with polymerase chain reaction from the genome of Chinese super rice cultivar Zhonghua 8. Physical map and DNA sequence analysis showed strong homology with the 5'-flanking region of rice prolamin gene reported by Kim in 1988. No changes in the signal peptide coding sequence and a long leader sequence with several small ORFs were found. Chimeric gene containing 5'-flanking region of the prolamin gene has been transcriptionally fused with the β-glucuronidase reporter gene. The fusion junction was confirmed by both physical map and DNA sequence analysis. The resultant chimeric gene was used to transform the tabacco explants by Ti binary system of Agrobacterium tumefaciens LBA4404. With dot and Southern blotting hybridization, three transgenic tobacco plants with the copies of chimeric GUS genes as many as 20 were obtained. Histochemical analysis revealed that the GUS activity in the endosperm tissues of tobacco seeds at the developmental stage was about 20 DAP. No GUS activity was found in leaves, stems, roots and flowers of the transgenic tobacco plants. Therefore, we concluded that the 5'-upstream cis-elements from—680 to—18 were enough to confer the endosperm-specific and temporal expression of rice prolamin gene.

关键词： β glueuronidase endosperm promoter transgenic tobacco riee prolamin.

Therapeutic molecules for multiple human diseases identified from pigeon pea(Cajanus cajan ***.)through GC–MS and molecular docking

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Food Science and Human Wellness 2017年第4期6卷 202-216页

作者： Deepu Mathew Lidiya John P. Manila T.M. Divyasree P. Sandhya Rajan V.T.K. Finishing School in biotechnology Centre for Plant Biotechnology and Molecular BiologyKerala Agricultural UniversityThrissurKerala 680656India

molecular mechanism behind the therapeutic potential of pigeon pea over the human diseases such as rheumatoid arthritis,breast cancer,type II diabetes,malaria,measles and sickle cell disease were revealed through docking of GC–MS identified phyto-compound ligands with candidate disease *** the 242 ligands,three dimensional structures of 47 compounds had to be drawn using ChemSketch and the remaining structures were retrieved from PubChem and docked with the active sites of candidate *** molecules identified through docking were further subjected to ADMET analysis and promising drug candidates were identified for each *** paper presents a precise account of the chemoprofile of pigeon pea leaves,stems and seeds,interaction of these molecules with target proteins and suggests 26 highly potential molecules which are drug candidates for multiple human *** pea seeds are especially proven as invaluable source for therapeutic molecules.

关键词： Breast cancer Drug discovery Herbal medicine In silico Malaria Measles Phyto-compounds Rheumatoid arthritis Sickle cell disease Type II diabetes

Characterization of lipid parameters in diabetic and non-diabetic atherosclerotic patients

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Journal of Geriatric Cardiology 2015年第1期12卷 37-43页

作者： Fatima Ali Hassan Jamil Sanam Saiqa Anwar Nadia Wajid Institute of molecular biology and biotechnology (IMBB) & centre for Research in molecular Medicine (CRIMM) University of Lahore 1-Kin Defence Road Lahore Pakistan

Background ＆ Objective The relationship between lipid profile perturbation and diabetes associated complications has long been an area of interest. Dyslipidemia is a potent predictor of cardiovascular morbidity and mortality in diabetic patients. The aim of present study was to investigate relationship between aging and lipid profiles in diabetic and non-diabetic atherosclerotie patients. Methods Five hundred and seventy six individuals （45-75 year age） participated in this study. Among these, 192 were having history of diabetes mellitus and atherosclerosis. Individuals are categorized on the base of health （normal, non-diabetic atheroselerosis, diabetic atherosclerosis） and age （45-55 years, 56~55 years, and 66-75 years）. All the participants were subjected to the procedures like a detailed history, biochemical analysis for fasting blood sugar, hemoglobin Alc, total cholesterol （TC）, triglycerides （TG）, low-density lipoprotein-（LDL）, very low-density lipoprotein （VLDL） and high-density lipoprotein （HDL）. All these parameters were compared between diabetic and non-diabetic atherosclerotic patients of all three age groups. TC/HDL and LDL/HDL were also calculated. Results Diabetic atherosclerotic individuals （both males and females） had high level of TC, TG, LDL, VLDL and low level of HDL in comparison to non-diabetic atherosclerotie and normal control individuals. Among all three age groups, lipoprotein abnormality was observed to be more frequent in females than males. There was a significant increase in TC/HDL and LDL/HDL ratio in diabetic atherosclerotic subjects compared to age and sex matched non-diabetic atherosclerotic and normal control. Conclusions Degree of dyslipidemia increases with increase in age in both genders. Female are more prone to diabetic dyslipidemia and hence have more risk of developing atherosclerosis with increasing age.

关键词： Aging Atherosclerosis Diabetes Dyslipidemia

Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2011年第4期12卷 326-334页

作者： Abdul Qayyum RAO Muhammad IRFAN Zafar SALEEM Idrees Ahmad NASIR Sheikh RIAZUDDIN Tayyab HUSNAIN National centre of Excellence in molecular biology

The phytochrome B (PHYB) gene of Arabidopsis thaliana was introduced into cotton through Agrobacterium *** and expression of PHYB gene in cotton plants were confirmed by molecular *** RNA (mRNA) expression in one of the transgenic lines,QCC11,was much higher than those of control and other transgenic *** cotton plants showed more than a two-fold increase in photosynthetic rate and more than a four-fold increase in transpiration rate and stomatal *** increase in photosynthetic rate led to a 46% increase in relative growth rate and an 18% increase in net assimilation *** recorded up to two generations,both in the greenhouse and in the field,revealed that overexpression of Arabidopsis thaliana PHYB gene in transgenic cotton plants resulted in an increase in the production of cotton by improving the cotton plant growth,with 35% more ***,the presence of the Arabidopsis thaliana PHYB gene caused pleiotropic effects like semi-dwarfism,decrease in apical dominance,and increase in boll size.

关键词： Transformation Gossypium hirsutum Phytochrome B Overexpression Plant growth Yield

Transgenic Tobacco Plants by Cotransformation With Proteinase Inhibitor Ⅱ and δ-endotoxin Genes

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Chinese Science Bulletin 1993年第18期38卷 1561-1566页

作者：华学军陈晓邦马万超范云六 molecular biology Laboratory Biotechnology Research Centre Chinese Academy of Agricultural Sciences Beijing 100080 PRC

In recent years, great progress has been made in. producing plants resistant to insect attack by genetic engineering of B. t δ-endotoxin gene or plant proteinase inhibitor genes, and how to avoid the emergence of the insect resistance to B. t δ-endotoxin produced by transgenic plants has been paid attention to. To combine one B. t δ-endotoxin gene with an incompatible B. t δ-endotoxin gene or with other genes such as proteinase inhibitor gene might alleviate the insect resistance problem. In this note, we study the

关键词： proteinase inhibitor gene B. t δ-endotoxin gene cotransformation transgenic plants gene expression.

RNA silencing movement in plants

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Journal of Integrative Plant biology 2016年第4期58卷 328-342页

作者： Glykeria Mermigka Frdric Verret Kriton Kalantidis Department of biology University of Crete Institute of molecular biology and biotechnology Foundation for Research and Technology

Multicellular organisms, like higher plants, need to coordinate their growth and development and to cope with environmental cues. To achieve this, various signal molecules are transported between neighboring cells and distant organs to control the fate of the recipient cells and organs. RNA silencing produces cell non-autonomous signal molecules that can move over short or long distances leading to the sequence specific silencing of a target gene in a well defined area of cells or throughout the entire plant,respectively. The nature of these signal molecules, the route of silencing spread, and the genes involved in their production, movement and reception are discussed in this review. Additionally, a short section on features of silencing spread in animal models is presented at the end of this review.

关键词： Cell non-autonomous post-transcriptional gene silencing RNA interference short range silencing spread systemic silencing plant RNA silencing transcriptional gene silencing

Therapeutic targeting of cellular prion protein: toward the development of dual mechanism anti-prion compounds

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Neural Regeneration Research 2025年第4期20卷 1009-1014页

作者： Antonio Masone Chiara Zucchelli Enrico Caruso Giovanna Musco Roberto Chiesa Laboratory of Prion Neurobiology Department of NeuroscienceIstituto di Ricerche Farmacologiche Mario Negri IRCCSMilanItaly Biomolecular NMR Unit Division of Genetics and Cell BiologyIRCCS Ospedale San RaffaeleMilanItaly Department of biotechnology and Life Sciences University of InsubriaVareseItaly

PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other *** adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective *** PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion *** this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of *** argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.

关键词： anti-prion drug anti-PrPC antibody antisense oligonucleotide neurodegeneration pharmacological chaperone porphyrin prion disease PrPC degrader PrPC shedding zinc finger repressor

Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan experience

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World Journal of Gastroenterology 2009年第40期15卷 5080-5085页

作者： Muhammad Idrees Shazia Rafi que Irshad-ur Rehman Haji Akbar Muhammad Zubair Yousaf Sadia Butt Zunaira Awan Sobia Manzoor Madiha Akram Mahwish Aftab Bushra Khubaib Sheikh Riazuddin Division of molecular Virology & molecular Diagnostics National Centre of Excellence in Molecular BiologyUniversity of the Punjab Lahore National centre of Excellence in molecular biology University of the Punjab Lahore

AIM: To assess the association between chronic hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC) in Pakistan, and the genotype distribution among these HCC ***: One hundred and sixty-one subjects with HCC were included in this study. Liver biopsy was performed on 145 of the patients; sixteen were excluded because they failed to fulfill the inclusion criteria. Qualitative polymerase chain reaction (PCR) was performed for hepatitis B virus and HCV. Samples positive for HCV RNA were genotyped using genotype-specif ic PCR and conf irmed by HCV 5' noncoding region sequencing analysis. RESULTS: Chronic HCV infection was identified a major risk factor (63.44% of tested HCC patients) forthe development of HCC. The time from HCV infection to appearance of cancer was 10-50 years. In the HCC patient population, broader distributions of genotypes were present with genotype 3a as the predominant genotype. Using the type-specific genotyping method, we found HCV genotype 3a in 40.96%, 3b in 15.66%, 1a in 9.63%, and 1b in 2.40% of HCC tissue samples. About 28% of cases were found with mixed genotypes. Two cases were unable to be genotyped because of low viral load. Sixty-six percent of treated patients with cirrhosis had an end of treatment response, but unfortunately they relapsed quickly when the treatment was discontin-ued, and HCC developed during a median 3.8 years. CONCLUSION: There was a strong association between chronic HCV infection and HCC in Pakistan, and between HCV genotype 3a and HCC.

关键词： Hepatocellular carcinoma Hepatitis C Genotyping Etiology Prevalence

Troponin I, cardiac diastolic dysfunction and restrictive cardiomyopathy

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Acta Pharmacologica Sinica 2004年第12期25卷 3-9页

作者： Xu-pei HUANG2, Jian-feng DU Department of Biomedical Science, Centerfor molecular biologyand biotechnology, Florida AtlanticUniversity, Boca Raton, FL 33431, USA Department of Biomedical Science Center for Molecular Biology and Biotechnology Florida Atlantic University 美国

Cardiomyopathies are diseases of heart muscle that are associated with cardiac dysfunction. molecular ge- netic studies performed to date have demonstrated that the damage or mutations in several sarcomeric contractile protein genes are associated with the development of the diseases. In this review, cardiac troponin I, one of the sarcomeric thin filament protein, will be discussed regarding its role in cardiac function, its deficiency-related diastolic dysfunction, and the mutation of this protein-mediated restrictive cardiomyopathy.

关键词： troponin I restrictive cardiomyopathy contractile proteins missense mutation diastole genetically modified animals cardiology