检索结果-南通市图书馆

Fecal microbiome of growing pigs fed a cereal based diet including chicory(Cichorium intybus L.) or ribwort(Plantago lanceolata L.) forage

引用

Journal of animal Science and Biotechnology 2016年第2期7卷 167-175页

作者： Johan Dicksved Janet K.Jansson Jan Erik Lindberg Department of animal Nutrition and Management Swedish University of Agricultural Sciences Department of Microbiology Swedish University of Agricultural Sciences division of Biology Earth and Biological Sciences Pacific Northwest National Laboratories

Background： The purpose of this study was to investigate how inclusion of chicory forage or ribwort forage in a cereal-based diet influenced the fecal microbial community（microbiome） in newly weaned（35 days of age） *** piglets were fed a cereal-based diet without（B） and with inclusion（80 and 160 g/kg air-dry forage） of vegetative shoots of chicory（C） and leaves of ribwort（R） forage in a 35-day growth trial. Fecal samples were collected at the start（D0）, 17（D17） and 35（D35） days after weaning and profiles of the microbial consortia were generated using terminal restriction fragment length polymorphism（T-RFLP）. 454-FLX pyrosequencing of 16 S r RNA gene amplicons was used to analyze the microbial composition in a subset of the samples already analyzed with ***： The microbial clustering pattern was primarily dependent on age of the pigs, but diet effects could also be observed. Lactobacilli and enterobacteria were more abundant at D0, whereas the genera Streptococcus, Treponema,Clostridium, Clostridiaceae1 and Coprococcus were present in higher abundances at D35. Pigs fed ribwort had an increased abundance of sequences classified as Treponema and a reduction in lactobacilli. However, the abundance of Prevotellaceae increased with age in on both the chicory and the ribwort diet. Moreover, there were significant correlations between the abundance of Bacteroides and the digested amount of galactose, uronic acids and total non-starch polysaccharides, and between the abundance of Bacteroidales and the digested amount of ***： This study demonstrated that both chicory and ribwort inclusion in the diet of newly weaned pigs influenced the composition of the fecal microbiota and that digestion of specific dietary components was correlated with species composition of the microbiota. Moreover, this study showed that the gut will be exposed to a dramatic shift in the microbial community structure several weeks after weaning.

关键词： Amplicon sequencing Chicory Microbiome Ribwort Uronic acid Weaning 16S

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

AMPK-ChREBP axis mediates de novo milk fatty acid synthesis promoted by glucose in the mammary gland of lactating goats

引用

animal Nutrition 2022年第3期10卷 234-242页

作者： Hengbo Shi Nannan Jiang Ling Wei Jie Cai Wenying Zhang Qianming Jiang Juan JLoor Jianxin Liu Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhou310058China College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou310018China Mammalian Nutrition Physiology Genomics Department of Animal Sciences and Division of Nutritional SciencesUniversity of IllinoisUrbanaIL61801USA

To investigate the role of glucose in regulating milk fatty acid synthesis,6 lactating Guanzhong dairy goats were infused with 0,60,or 100 g/d glucose via the external pubic artery in a 33 repeated Latin square experiment.A concomitant in vitro experiment was conducted to investigate possible mechanisms whereby glucose regulates milk fatty acid *** sequencing was used for cellular transcriptome ***,MK-2206,rapamycin,and dorsomorphin were used to block cellular mammalian AMP-activated protein kinase(AMPK),AKT serine/threonine kinase 1,and mechanistic target of rapamycin kinase signaling pathways,*** response element binding protein(ChREBP)was knockdown and overexpressed to investigate its role in regulating milk fatty acid synthesis in mammary epithelial *** infusion linearly elevated the concentration of C8:0(P=0.039)and C10:0(P=0.041)in milk fat while it linearly decreased(P=0.049)that of C16:*** result was in agreement with the upregulation of genes related to de novo synthesis of fatty acids and lipid droplet formation,including adipose differentiation-related protein,butyrophilin subfamily 1 member A1,fatty acid synthase(FASN)and *** expression increased(P<0.05)linearly in the lactating goat mammary *** vitro,glucose linearly stimulated the expression of genes related to de novo synthesis of fatty acids and cellular triacylglycerol in cultured mammary epithelial *** sequencing and inhibition studies revealed that glucose induced transcriptomic changes increasing lipogenic pathways,with AMPK responding to glucose by controlling ChREBP and *** and overexpression of ChREBP highlighted its essential role in *** knockdown and overexpression of ChREBP protein also revealed an essential role in regulating the de novo synthesis of fatty ***,our data highlight that glucose supplementation promotes de novo fatty acid synthesis via the AMPK-ChREBP axis,hence i

关键词： Milk fat Glucose infusion Mammary gland Carbohydrate response element binding protein De novo synthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Antioxidant potential of pentoxifylline on spermatozoa of small ruminants

引用

Asian pacific Journal of Reproduction 2022年第3期11卷 138-145页

作者： Mazen Alomar division of animal Production Department of AgricultureAtomic Energy CommissionP.O.Box 6091DamascusSyria

Objective:To investigate antioxidant potential of pentoxifylline on spermatozoa of small ruminants including rams and ***:The levels of hydrogen peroxide(H2O2)production in ram and buck spermatozoa incubated with 0(control),4 and 8 mM of pentoxifylline were measured after 45-min ***,the motility parameters of ram and buck spermatozoa incubated with 0(control),1 mM of H2O2,1 mM of H2O2 plus 4 mM of pentoxifylline,and 1 mM of H2O2 plus 8 mM of pentoxifylline were analysed.H2O2 was estimated using a fluorometric assay and spermatozoa motility characteristics were analyzed using computer aided sperm ***:Pentoxifylline significantly decreased the levels of H2O2 produced from both ram and buck spermatozoa(P<0.05),and significant lower rates of H2O2 formation were noted when 8 mM of pentoxifylline was added(P<0.05).The values of all sperm motility parameters for the two species significantly decreased after incubation with H2O2(P<0.05).In contrast,when the spermatozoa were incubated with both H2O2 and two concentrations of pentoxifylline,the motility values rose significantly compared to those incubated with H2O2 alone(P<0.05).For both ram and buck sperm samples,the rapid and static subpopulation motility parameters were the most affected categories by pentoxifylline addition compared to the medium and slow ***:Pentoxifylline possesses an antioxidant capacity proved by its ability of reducing H2O2 levels as well as by increasing motility values of stressed ***,pentoxifylline could be recommended as antioxidant additive for spermatozoa of small ruminants under stress conditions.

关键词： Pentoxifylline Spermatozoa Ram Buck Motiltiy Hydrogen peroxide H2O2 Antioxidants

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of flight transport stress on blood parameters in beagles and the anti-stress effect of dangshen

引用

animal Models and Experimental Medicine 2018年第2期1卷 162-168页

作者： Wang Liang Ling-Yu Zeng Chuan-Xin Yu Xiu-Xiu Gao Lu-Di Fu Xiao Wang Laboratory animal Center Guangzhou University of Traditional Chinese Medicine Acupuncture division Meizhou Second Chinese Medicine Hospital

Background: This study investigated the effect of flight transport stress on beagles' routine blood indexes and biochemical parameters and evaluated the anti-stress effect of dangshen(Codonopsis pilosula).Methods: We selected 12 beagles and divided them into two groups. One group was treated with dangshen decoction two hours before the flight, and the other group was untreated. Their routine blood indexes and clinical biochemical parameters were tested and analyzed before transport, after unloading and after adaptation for 1, 2, 3, 4, 5, and 6 days after administering ***: We found that flight transportation stress adversely influenced many of the beagles' routine blood indexes. These recovered during adaptation, with dangshen administration assisting recovery of most indexes. Flight transport stress also adversely influenced biochemical indexes in the beagles. Again these recovered during adaptation, and dangshen aided in the ***: Thus, we found that flight transport adversely affected the beagles' blood indexes, and dangshen reversed the damage from transport stress.

关键词： adaptive breathing anti-stress beagle dog dangshen flight

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Differential miRNA expression profiling of highly pathogenic avian influenza virus H5N1 infected chicken lungs reveals critical microRNAs,biological pathways and genes involved in the molecular pathogenesis

引用

Virologica Sinica 2022年第3期37卷 465-468页

作者： Anamika Mishra Muhasin Asaf Amod Kumar Diwakar Dattatreya Kulkarni Richa Sood Sandeep Bhatia Bharat Bhushan Ashwin Ashok Raut Pathogenomics Laboratory ICAR-National Institute of High Security Animal DiseasesOIE referral laboratory for Avian InfluenzaBhopal 462021India ICAR-National Institute of High Security animal Diseases OIE referral lab for Avian InfluenzaBhopal 462021India division of animal Genetics ICAR-Indian Veterinary Research InstituteBareilly 243122India

Dear Editor,Avian influenza is a highly contagious viral infection affecting the respiratory ***(miRNAs)are small,regulatory,endogenous,non-coding RNAs of~22 nt that regulate the gene expression of the target mRNAs by cleavage or translational *** are connected with the host response during avian influenza virus(AIV)infection(Wang et al.,2009,2012).In this study.

关键词： influenza endogenous pathogenesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phylogenetic analysis and characterization of mitochondrial DNA for Korean native cattle

引用

Open Journal of Genetics 2013年第1期3卷 12-23页

作者： Hoyoung Chung animal genomics & Bioinformatics division National Institute of Animal Science Suwon Korea

This study was aimed to preserve mitochondria from liver tissues of Korean native cattle (KNC) as genetic resources and to analyze phylogenetic relationships among cattle breeds using the whole sequences of mtDNA. Mitochondria of KNC has been isolated with the alkaline lysis procedure using1 gof liver tissues, and measurements showed numbers of cells (2.64 × 1010), concentration (114.6 ug/ml), proteins (0.278 ug/ml), and absorbance (0.029) in 260 nm. Mitochondrial DNAs (mtDNA) were extracted from the isolated mitochondria to determine whole sequences that can be used to estimate genetic distances among cattle breeds. Designing of primers based on the bovine mtDNA sequence was resulted in 16 primer sets covering the whole mtDNA regions. The analysis of 40 KNC mtDNA sequences revealed 69 polymorphic sites that were less than the average number of SNP (single nucleotide polymorphism) for other populations (82 sites) in this study. As expected, the highest observation of SNPs was found in the D-loop region, and a total of 29 SNPs were shared between cattle populations. The haplotype analysis, which used 13 SNPs located in D-loop, COX2, ND5, CYTB, and non coding regions, revealed that KNC showed clearly different haplotypes that may be used to distinguish KNC from other breeds as well as to characterize individual identifications. The phylogenetic analysis revealed that KNC showed a relatively close genetic distance with Japanese black cattle (JBC) comparing with other breeds. Estimations of the average nucleotide diversity (0.0008) and the ratio of transition/ transversion (0.1050) of KNC were placed somewhere in the middle of genetic diversity among cattle breeds. The identified genetic variants and results of phylogenetic analysis will help understanding the phylogenetic relationships of KNC and provide useful genetic information for further studies regarding SNPs in mtDNA regions.

关键词： mtDNA Cattle SNP Haplotype Genetic Distance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

引用

animal Nutrition 2021年第1期7卷 206-215页

作者： Yanfen Ma Ying Feng Liwen Song Muyang Li Hongyu Dai Hua Bao Guijie Zhang Lei Zhao Chunhua Zhang Jing Yi Yusheng Liang School of Agriculture Ningxia UniversityYinchuan 750021China Institute of animal Nutrition and Feed Inner Mongolia Academy of Agriculture and Animal Husbandry SciencesHohhot 010031China College of Food Engineering&Biotechnology Tianjin University of Science&TechnologyTianjin 300457China College of animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityDaqing 163316China College of Veterinary Medicine Nanjing Agricultural UniversityNanjing 210095China Department of animal Sciences and division of Nutritional Sciences University of IllinoisUrbana 61801USA

Peripartal cows often experience negative energy balance,and are therefore prone to suffering from metabolic diseases such as hyperketonemia,which causes financial losses in dairy *** study aimed to investigate the effect of green tea polyphenol(GTP)supplementation during the periparturient period on production performance,oxidative stress and immunometabolism in dairy cows with *** hundred Holstein cows were assigned to GTP(0.2 g/kg DM;n=50)or control(without GTP;n=50)group based on body weight,previous milk yield,and parity on d 15 before expected ***,10 cows with hyperketonemia were selected from each group,according to bloodβ-hydroxybutyric acid(BHBA)concentration between 1.2 and 2.9 mmol/L from 2 to 3 d *** cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d *** and blood samples were obtained from 20 cows selected with hyperketonemia on 10,20,and 30 d *** with control cows,greater milk yield and lower somatic cell count were observed in GTP *** GTP group had lower concentrations of BHBA,free fatty acids,cholesterol,triglyceride,reactive oxygen species,malondialdehyde,and hydrogen peroxide,greater concentrations of glucose,lower activities of aspartate aminotransferase,alanine aminotransferase,and glutamyl transpeptidase,alongside greater ac-tivities of superoxide dismutase,glutathione peroxidase,and total antioxidant ***,GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10,but down-regulated concentrations of tumor necrosis factor-α,interleukin-1β,interleukin-2,interleukin-8,and interferon-γin *** concentrations of plasma immunoglobulin G were also detected in the GTP ***,the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hvoerketonemia during early lactation.

关键词： Green tea polyphenol Hyperketonemia Peripartal period Dairy cow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Trigonella foenum-graecum seeds extract plays a beneficial role on brain antioxidant and oxidative status in alloxan-induced Wistar rats

引用

Food Quality and Safety 2020年第2期4卷 83-89页

作者： Jangampalli Adi Pradeepkiran Venkata Subbaiah Nandyala Matcha Bhaskar Department of Zoology Division of Animal BiotechnologySri Venkateswara UniversityTirupatiIndia Department of Internal Medicine Texas Tech University of Health Science CentreLubbockUSA

Context:Trigonella foenum-graecum(TriFG)exhibits increased scavenger enzymatic activities and reduces the production of reactive oxygen species in diabetic ***:The present study was aimed to investigate the effect of TriFG on lipid peroxidation levels and antioxidant status in brain tissue of rats exposed to *** and Methods:Healthy male rats(180±10 g)were allocated into five *** in group 1 maintained on normal tap water served as controls and rats in groups 2,3,4,and 5 were treated as experimental *** in group 2 were intraperitoneally injected with alloxan(120 mg/kg BW)and treated as diabetic rats,whereas rats in groups 3 and 4 were maintained on same experimental regimen as that of rats in groups 1 and 2,respectively,and in addition,they were orally gavaged with herbal extracts of TriFG(0.25 g/kg BW).Diabetic rats treated with glibenclamide in group 5 were used as positive *** and Discussion:Significant(P<0.001)increase in the antioxidant enzymes with a significant(P<0.001)decrease in the lipid peroxidation levels were observed in the brain tissue of diabetic rats treated with TriFG extract as compared to diabetic and glibenclamide-treated *** significant changes were observed in pro-and antioxidant levels in brain tissue of rats treated with TriFG extract alone when compared to normal *** diabetic rats,brain mitochondrial and cytosolic enzymes like succinate dehydrogenase,glutamate dehydrogenase,and glucose-6-phosphate dehydrogenase activity levels were significantly(P<0.05)decreased with reversely increased was observed in lactate dehydrogenase activity(P<0.05).Conclusions:The findings of the present study suggested that TriFG,through its antioxidant properties,protects brain tissue by mitigating oxidative stress induced by alloxan-exposed *** extract significantly increased the antioxidant and oxidative properties in diabetic rats when compared with the control group rats.

关键词： Trigonella foenum-graecum hyperglycaemia brain antioxidants rats

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Amphibian and reptilian distribution patterns in the transitional zone between the Euro-Siberian and central Asia Subrealms

引用

Journal of Arid Land 2015年第4期7卷 555-565页

作者： ZHOU Lu SHI Lei College of animal Science Xinjiang Agricultural University

There exist some controversies over the larger zoogeographic divisions of the arid areas of central Asia, whose characteristics include complex ecological environments, complex fauna origins and unique patterns of animal distribution. The aim of this study was to determine, using quantitative analysis, the distribution patterns of amphibians and reptiles in the arid areas of central Asia, whose various physiographical regions were divided into 17 Operative Geographical Units （OGUs）. Based on the presence or absence of 52 amphibian and reptile genera in the 17 OGUs, and by making use of the Czekanowski Similarity Index, the Baroni-Urbani and Buser＇s Similarity Index, and the strong and weak boundary test, we studied the biotic boundaries within these contested regions. In accordance with our results, the classification dendrogram was divided into two main branches. One branch is composed of the northern OGUs of the Altai Mountains which are a part of the Euro-Siberian Subrealm. The other branch includes all of the OGUs south of the Altai Mountains, which belong to the central Asia Subrealm. There is a significantly weak biotic boundary （DW〉0, GW〉GS, P〈0.01） between the Euro-Siberian Subrealm and the central Asia Subrealm that corre- sponded to the transitional zones. The boundary between the two subrealms runs along the Altai Mountains, the Sayan Mountains, the Hangai Mountains and the Mongolian Dagurr Mountains. The boundaries between the main branches in the central Asia Subrealm are weak, reflecting the widespread existence of transitional zones in the arid areas of central Asia. The Tianshan Mountains should be elevated to form its own separate region, ＂the Middle Asian Mountain Region＂, which, due to its special fauna and environment, would be classified at the same level as the Mongolia-Xinjiang Region. With the approach of creating a cluster analysis dendogram based upon the genera of amphibians and reptiles, the relationship of these higher level zoogeographi

关键词： amphibian and reptile distribution pattern higher level zoogeographic division similarity analysis boundary test arid area central Asia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Reactive oxygen and nitrogen species regulate porcine embryo development during pre-implantation period:A mini-review

引用

animal Nutrition 2021年第3期7卷 823-828页

作者： Zhen Luo Jianbo Yao Jianxiong Xu School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai Key Laboratory of Veterinary BiotechnologyShanghaiChina division of animal and Nutritional Sciences West Virginia UniversityMorgantownWVUSA

Significant porcine embryonic loss occurs during conceptus morphological elongation and attachment from d 10 to 20 of pregnancy,which directly decreases the reproductive efficiency of sows.A successful establishment of pregnancy mainly depends on the endometrium receptivity,embryo quality,and utero-placental microenvironment,which requires complex cross-talk between the conceptus and *** understanding of the molecular mechanism regulating the uterine-conceptus communication during porcine conceptus elongation and attachment has developed in the past *** oxygen and nitrogen species,which are intracellular reactive metabolites that regulate cell fate decisions and alter their biological functions,have recently reportedly been involved in porcine conceptus elongation and *** mini-review will mainly focus on the recent researches about the role of reactive ox-ygen and nitrogen species in regulating porcine embryo development during the pre-implantation period.

关键词： Reactive oxygen and nitrogen species Pre-implantation Porcine Embryo development

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：