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Journal of Integrative Agriculture 2014年第9期13卷 1900-1908页

作者： AN Xia DUAN Feng-ying GUO Song CHEN Fan-jun YUAN Li-xing GU Ri-liang Department of Plant Nutrition Center for ResourcesEnvironment and Food SecurityChina Agricultural University

Aldehyde dehydrogenases（ALDHs） represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of the ALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maize ALDH7 subfamily member（ZmALDH7B6） was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance of ZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-specific and stress-induced expression patterns, the 1.5-kb 5′-flanking ZmALDH7B6 promoter region was fused to the β-glucuronidase（GUS） reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was significant induction of GUS activity in response to ammonium supply, confirming ammonium-dependent expression of ZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by the ZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested that ZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.

关键词： abiotic stress aldehyde dehydrogenase gene expression promoter transgenic maize

Effects of slab geometry and obliquity on the interplate thermal regime associated with the subduction of three-dimensionally curved oceanic plates

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Geoscience Frontiers 2015年第1期6卷 61-78页

作者： Yingfeng Ji Shoichi Yoshioka Research center for Urban Safety and security Kobe University Department of Earth and planetary Science Graduate School of ScienceKobe University

We investigated the relationships among slab geometry, obliquity, and the thermal regime associated with the subduction of oceanic plates using a three-dimensional （3D） parallelepiped thermal convection model. Various models with convex and concave slab shapes were constructed in the numerical simu- lation, and the temperature and mantle flow distributions were calculated. The results revealed that when the slab dip angle increases, or the obliquity of subduction becomes steeper, the interplate tem- perature decreases remarkably. Cooler （warmer） zones on the plate interface were identified from the modeling where there was a larger （smaller） subduction angle. Consequently, the interplate temperature distribution is partly controlled by the true subduction angle （TSA）, which is a function of the slab dip angle and the obliquity of subduction. The rate of change of the interface temperature for the TSA was 10-50 ℃ （10°〈 TSA 〈 20°） at depths ranging from （TSA 10） × 5 km to 60 ＋（TSA 10） × 5 km for a fiat slab after a subduction history of 7 Myrs. The along-arc slab curvature affects the variation in TSA. The slab radius also appeared to influence the radius of induced mantle flow.

关键词： Temperature Mantle flow Heat flow Numerical simulation Slab geometry Obliquity

Inorganic arsenic contamination of rice from Chinese major rice-producing areas and exposure assessment in Chinese population

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Science China Chemistry 2015年第12期58卷 1898-1905页

作者： Xiaowei Li Ke Xie Bing Yue Yunyun Gong Yi Shao Xiaohong Shang Yongning Wu Key Laboratory of food Safety Risk Assessment Ministry of Health China National center for food Safety Risk Assessment 3. food Science and Engineering Department Wuhan Polytechnic University 4. Institute for Global Food Security School of Biological Sciences Queen's University of Belfast

This study examined the total arsenic(Ast) and inorganic arsenic(Asi) content in the main rice growing area of China. The results were compared with other countries and then used for dietary exposure assessment. A total of 446 rice samples from 15 main rice-growing provinces and autonomous regions of China were collected and then divided into unpolished and polished rice. Total arsenic and arsenic species were analyzed in a total of 892 subsamples using inductively coupled plasma-mass spectrometry(ICP-MS) and high performance liquid chromatography(HPLC) coupled ICP-MS, respectively. National Ast means were 255 μg/kg of unpolished rice and 143 μg/kg of polished rice. Asi was found to be the predominant species and mean levels were 209 μg/kg of unpolished rice and 108 μg/kg of polished rice, respectively. Exposure assessment to Asi in polished rice has been calculated for the margin of exposure(MOE), which highlights the fact that Asi levels in the Chinese rice should arouse public health concern.

关键词： arsenic speciation exposure assessment inorganic arsenic rice

Cyanidin-3-O-galactoside and Blueberry Extracts Supplementation Improves Spatial Memory and Regulates Hippocampal ERK Expression in Senescence-accelerated Mice

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Biomedical and Environmental Sciences 2014年第3期27卷 186-196页

作者： TAN Long YANG Hong Peng PANG Wei LU Hao HU Yan Dan LI Jing LU Shi Jun ZHANG Wan Qi JIANG Yu Gang Department of Nutrition and food security School of Public HealthTianjin Medical University Department of Nutrition and food Science Institute of Health and Environmental MedicineAcademy of Military Medical Sciences Tianjin Agricultural College center for Disease Control and Prevention PLA Chengdu Military Area Command

Objective To investigate whether the antioxidation and the regulation on the Extracellular Regulated Protein Kinases （ERK） signaling pathway are involved in the protective effects of blueberry on central nervous system. Methods 30 Senescence-accelerated mice prone 8 （SAMP8） mice were divided into three groups and treated with normal diet, blueberry extracts （200 mg/***/day） and cyaniding-3-O-galactoside （Cy-3-GAL）（50 mg/***/day） from blueberry for 8 weeks. 10 SAMR1 mice were set as control group. The capacity of spatial memory was assessed by Passive avoidance task and Morris water maze. Histological analyses on hippocampus were completed. Malondialdehyde （MDA） levels, Superoxide Dismutase （SOD） activity and the expression of ERK were detected. Results Both Cy-3-GAL and blueberry extracts were shown effective functions to relieve cellular injury, improve hippocampal neurons survival and inhibit the pyramidal cell layer damage. Cy-3-GAL and blueberry extracts also increased SOD activity and reduced MDA content in brain tissues and plasma, and increased hippocampal phosphorylated ERK （p-ERK） expression in SAMP8 mice. Further more, the passive avoidance task test showed that both the latency time and the number of errors were improved by Cy-3-GAL treatment, and the Morris Water Maze test showed significant decreases of latency were detected by Cy-3-GAL and blueberry extracts treatment on day 4. Conclusion Blueberry extracts may reverse the declines of cognitive and behavioral function in the ageing process through several pathways, including enhancing the capacity of antioxidation, altering stress signaling. Cy-3-GAL may be an important active ingredient for these biological effects.

关键词： Blueberry Senescence-accelerated mice Cognition Cyanidin-3-O-galactoside ERK Oxidation

Technical and environmental efficiency of hog production in China—A stochastic frontier production function analysis

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Journal of Integrative Agriculture 2015年第6期14卷 1069-1080页

作者： ZHOU Ying-heng ZHANG Xiao-heng TIAN Xu GENG Xian-hui ZHANG Peng YAN Bin-jian College of Economics and Management Nanjing Agricultural University China center for food security Studies Nanjing Agricultural University

This article analyses the technical and environmental efficiency of hog production in China using data from the China Agricultural Product Cost-Benefit Compilation（NDRC 2005–2013） and the First National Census of Pollution： Manual of Discharge Coefficient of Livestock and Poultry Industry（IEDA and NIES 2009）. The empirical results show a great variation in environmental efficiency, ranging from 0.344 to 0.973 with a mean value of 0.672 that declines over time. Southwest China is found to be the most environmentally efficient region, while the Northeast and the Northwest are the least efficient. Another finding is that technical and environmental efficiencies are highly correlated in hog production; the most environmentally efficient regions are usually found to have high technical efficiency, and vice versa. In addition, we computed the output elasticities with respect to each factor input. The results show that feed is the most efficient input, with an output elasticity of approximately 0.551, which is much higher than the elasticity of the nitrogen surplus, other capital or labour. The output elasticity with respect to the nitrogen surplus is 0.287 on average. Finally, the scale elasticity in hog production is slightly higher than 1.

关键词： frontier surplus elasticity Northeast stochastic Census Northwest finding technological producer

China's meat and grain imports during 2000–2012 and beyond: A comparative perspective

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Journal of Integrative Agriculture 2015年第6期14卷 1101-1114页

作者： YU Wu-sheng CAO Li-juan Department of food and Resource Economics University of Copenhagen College of Economics and Management Nanjing Agricultural University China center for food security Research Nanjing Agricultural Universtiy

This paper provides a review on China＇s meat trade for the 2000–2012 period and discusses its future development, with reference to China＇s grain trade. With marginal decreases in meat exports and slight increases in their imports, China＇s net imports of major meat products（including pork, beef, mutton and poultry but excluding meat offal） were just below 1 million tons in 2012, dwarfed by China＇s net imports of grains which reached 66.7 million tons in the same year. This slow growth in meat trade seems to contradict earlier expectations on increasing meat demand and imports, based upon projected shifts in consumption patterns driven by rapid per capita income growth. Several plausible explanations of this paradoxical trade pattern are offered, including mass imports of feed grains, persistent（but shrinking） gaps between Chinese and international meat prices, tariff barriers, and non-tariff measures. In the near future China may not be able to maintain such a lower profile on the world meat markets, as per capita income is projected to continue to rise and domestic production cost advantages erode due to rising labor costs. A model-based projection exercise indicates that under plausible assumptions China＇s meat imports may rise sharply by 2030.

关键词： imports and beyond tariff prices capita income excluding earlier projection quota

Varietal Differences in Plant Growth, Phosphorus Uptake and Yield Formation in Two Maize Inbred Lines Grown Under Field Conditions

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Journal of Integrative Agriculture 2012年第10期11卷 1738-1743页

作者： CHEN Fan-jun LIU Xiang-sheng MI Guo-hua Key Laboratory of Plant-Soil Interactions Ministry of Education/Center for Resources Environment and Food Security China Agricultural University

Selection for phosphorus （P）-efficient genotypes and investigation of physiological mechanisms for P-use efficiency in maize has mainly been conducted at the seedling stage under controlled greenhouse conditions. Few studies have analyzed characteristics of plant growth and yield formation in response to low-P stress over the whore growth period under field conditions. In the present study, two maize inbred lines with contrasting yield performances under low-P stress in the field were used to compare plant growth, P uptake and translocation, and yield formation. Phosphorus accumulation in the P-efficient line 154 was similar to that of line 153 under high-P. Under low-P, however, P uptake in line 154 was three times greater than that in line 153. Correspondingly, P-efficient line 154 had a significantly higher yield than P-inefficient line 153 under low-P conditions （Olsen-P=1.60 mg kg-1）, but not under high-P conditions （Olsen-P=14.98 mg kg-1）. The yield difference was mainly due to differences in the number of ears per m2, that is, P-efficient line 154 formed many more ears under low-P conditions than P-inefficient line 153. Ear abortion rate was 53% in the P-inefficient line 153, while in line 154, it was only 30%. Low-P stress reduced leaf appearance, and delayed anthesis and the silking stage, but increased the anthesis-silking interval （ASI） to a similar extent in both lines. The maximum leaf area per plant at silking stage was higher in P-efficient line 154 than in P-inefficient line 153 under both P conditions. It is concluded that low-P stress causes intense intraspecific competition for limited P resources in the field condition which gives rise to plant-to- plant non-uniformity, resulting in a higher proportion of barren plants. As soon as an ear was formed in the plant, P in the plant is efficiently reutilized for kernel development.

关键词： ear abortion leaf growth low phosphorus maize phosphorus uptake

Towards sustainable intensification of apple production in China—Yield gaps and nutrient use effi ciency in apple farming systems

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Journal of Integrative Agriculture 2016年第4期15卷 716-725页

作者： WANG Na Joost Wolf ZHANG Fu-suo Plant Production Systems Department of Plant Sciences Wageningen University center for Resources Environment and Food Security China Agricultural University

China is in a dominant position in apple production globally with both the largest apple growing area and the largest export of fresh apple fruits. However, the annual productivity of China＇s apple is significantly lower than that of other dominant apple producing countries. In addition, apple production is based on excessive application of chemical fertilizers and the nutrient use efficiency （especially nitrogen） is therefore low and the nutrient emissions to the environment are high. Apple production in China is considerably contributes to farmers＇ incomes and is important as export product. There is an urgent need to enhance apple productivity and improve nutrient use efficiencies in intensive apple production systems in the country. These can be attained by improved understanding of production potential, yield gaps, nutrient use and best management in apple orchards. To the end, priorities in research on apple production systems and required political support are described which may lead to more sustainable and environmental-friendly intensification of apple production in China.

关键词： apple production China environmental problems nutrient use efficiency potential yield sustainableintensification yield gaps

Responses of Aerobic Rice (Oryza sativa L.) to Iron Deficiency

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Journal of Integrative Agriculture 2012年第6期11卷 938-945页

作者： SHI Rong-li HAO Hong-mei FAN Xiao-yun Md Rezaul Karim ZHANG Fu-SUO ZOU Chun-qin Key Laboratory of Plant-Soil Interaction Ministry of Education/Center for ResourcesEnvironment and Food SecurityDepartment of Plant NutritionChina Agricultural University

Aerobic rice has the advantage of saving water. Most published work has focused on improving its yield, while few reported on its micronutrient status. In fact, Fe deficiency is a common nutritional problem in the production of aerobic rice. Short- term hydroponic culture experiments were conducted to study the response of aerobic rice to Fe deficiency and the effect of root exudates from Fe-deficient wheat on its Fe uptake ability. The results indicate that the amount of phytosiderophores （PS） released from aerobic rice did not increase under Fe deficient conditions. The Fe（III） reducing capacity of Fe-deficient aerobic rice did not increase and the solution pH did not decrease significantly. What＇s more, no obvious swelling was observed in the root tips. Aerobic rice did not show special responses to improve their Fe nutrition under Fe deficiency as both strategy I and II plants though they were very sensitive to Fe deficiency. This may be a reason which causes Fe deficiency problem in aerobic rice. However, root exudates from Fe-deficient wheat （PSw） could improve its Fe nutrition in the presence of insoluble Fe（OH）3. This suggests that aerobic rice could utilize Fe activated by PSw.

关键词： aerobic rice Fe(III)-reduction iron nutrition phytosiderophore release root tip swelling

Genetic Improvement of Root Growth Contributes to Efficient Phosphorus Acquisition in maize (Zea mays L.)

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Journal of Integrative Agriculture 2013年第6期12卷 1098-1111页

作者： ZHANG Yi-kai CHEN Fan-jun CHEN Xiao-chao LONG Li-zhi GAO Kun YUAN Li-xing ZHANG Fu-suo MI Guo-hua Laboratory of Plant-Soil Interactions Ministry of Education/Center for Resources Environment and Food Security College of Resources and Environmental Science China Agricultural University

Maize plants adapt to low phosphorus （P） stress by increasing root growth. It is of importance to know the extent to which genetic improvement of root growth can enhance P acquisiton. In the present study, the contribution of root growth improvement to efficient P acquisition was evaluated in two soils using T149 and T222, a pair of near isogenic maize testcrosses which were derived from a backcross BC 4 F 3 population. T149 and T222 showed no difference in shoot biomass and leaf area under normal growth conditions, but differed greatly in root growth. T149 had longer lateral roots and a larger root surface area compared to T222. In calcareous soil, when P was insufficient, i.e., when P was either supplied as KH 2 PO 4 at a concentration of 50 mg P kg-1 soil, or in the form of Phy-P, Ca3-P or Ca10-P, a 43% increase in root length in T149 compared to T222 resulted in an increase in P uptake by 53%, and shoot biomass by 48%. In acid soil, however, when P supply was insufficient, i.e., when P was supplied as KH 2 PO 4 at a concentration of 100 mg P kg-1 soil, or in the form of Phy-P, Fe-P or Al-P, a 32% increase in root length in T149 compared to T222 resulted in an increase in P uptake by only 12%, and shoot biomass by 7%. No significant differences in the exudation of organic acids and APase activity were found between the two genotypes. It is concluded that genetic improvement of root growth can efficiently increase P acquisition in calcareous soils. In acid soils, however, improvements in the physiological traits of roots, in addition to their size, seem to be required for efficient P acquisition.

关键词： P efficiency roots low phosphorus calcareous soil acid soil maize