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Development and application of microsatellite markers within transcription factors in flare tree peony(Paeonia rockii)based on next-generation and single-molecule long-read RNA-seq

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Journal of Integrative Agriculture 2021年第7期20卷 1832-1848页

作者： LIU Na CHENG Fang-yun GUO Xin ZHONG Yuan Beijing Advanced Innovation center for Tree Breeding by Molecular Design/Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research center for Floriculture/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education/peony International Institute School of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083P.R.China

Tree peonies native to China are a precious crop with ornamental,medicinal and edible oil properties,of which flare tree peony(Paeonia rockii)is one of the most significant germplasms in *** development and application of expressed sequence tag-simple sequence repeat(EST-SSR)markers are very valuable for genetic and breeding applications,but EST-SSR resources for the genus Paeonia are still *** this study,we first reported the development of SSRs within transcription factors(TFs)in *** based on next-generation sequencing(NGS)and single-molecule long-read sequencing(SMLRS).A total of 166 EST-SSRs containing six nucleotide repeat types were identified from 959 candidate TFs associated with yield,with an average of one SSR per 5.83 *** total,102(61.45%)pairs of primers produced amplification products in the two RNA-seq *** them,58(56.86%)pairs of primers from 18 gene families(AP2,b HLH,HSF,etc.)were identified to be polymorphic both in the parents of a linkage mapping population and in eight randomly selected accessions of ***,the 58 EST-SSRs indicated a high level of informativeness with PIC values ranging from 0.32 to 0.91(mean 0.70)after assessment in 37 tree peony *** studies indicated that the amplification ratio of the 58 pairs of primers ranged from 89.66 to 100%across seven species of *** addition,a genetic relationship study was performed in 62 *** analysis using the neighbour-joining(NJ)tree demonstrated that major clusters corresponded to the known pedigree *** together,these newly developed EST-SSRs have a potential use in the conservation of tree peony germplasm and marker-assisted selection(MAS)breeding.

关键词： flare tree peony(Paeonia rockii) EST-SSR markers polymorphism genetic diversity transferability genetic relationships

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Arsenic release from microbial reduction of scorodite in the presence of electron shuttle in flooded soil

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Journal of Environmental Sciences 2023年第4期126卷 113-122页

作者： Yujuan Fang Manjia Chen Chengshuai Liu Leheng Dong Jimei Zhou Xiu Yi Dongqing Ji Jiangtao Qiao Hui Tong School of Water and Environment Chang’an UniversityXi’an 710054China National-Regional Joint Engineering Research center for Soil Pollution Control and Remediation in South China Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and ManagementInstitute of Eco-environmental and Soil SciencesGuangdong Academy of SciencesGuangzhou 510650China State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of SciencesGuiyang 550081China College of Agriculture/Tree peony Henan University of Science and TechnologyLuoyang 471023China

Scorodite (FeAsO_(4)·H_(2)O) is a common arsenic-bearing (As-bearing) iron mineral in nearsurface environments that could immobilize or store As in a bound *** flooded soils,microbe induced Fe(Ⅲ) or As(Ⅴ) reduction can increase the mobility and bioavailability of ***,humic substances can act as electron shuttles to promote this *** dynamics of As release and diversity of putative As(Ⅴ)-reducing bacteria during scorodite reduction have yet to be investigated in detail in flooded ***,the microbial reductive dissolution of scorodite was conducted in an flooded soil in the presence of anthraquinone-2,6-disulfonate (AQDS).Anaeromyxobacter,Dechloromonas,Geothrix,Geobacter,Ideonella,and Zoogloea were found to be the dominant indigenous bacteria during Fe(Ⅲ) and As(Ⅴ) *** increased the relative abundance of dominant species,but did not change the diversity and microbial community of the systems with *** these bacteria,Geobacter exhibited the greatest increase and was the dominant Fe(Ⅲ)-and As(Ⅴ)-reducing bacteria during the incubation with AQDS and *** promoted both Fe(Ⅲ) and As(Ⅴ) reduction,and over 80%of released As(Ⅴ) was microbially transformed to As(Ⅲ).The increases in the abundance of arrA gene and putative arrA sequences of Geobacter were higher with AQDS than without *** a result,the addition of AQDS promoted microbial Fe(Ⅲ) and As(Ⅴ) release and reduction from As-bearing iron minerals into the *** results contribute to exploration of the transformation of As from As-bearing iron minerals under anaerobic conditions,thus providing insights into the bioremediation of As-contaminated soil.

关键词： Scorodite Fe(III)reduction As(V)reduction Arsenate reductase gene(arrA) AQDS

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Outdoor Domestication Cultivation and Survival Mechanism for Tissue Culture Seedlings of Paeonia suffruticosa

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Asian Agricultural Research 2019年第8期11卷 60-63页

作者： Xianglong MA Jingxu WU Yixin LIANG Shaohua LIU Yongmei WANG Luoyang peony Development center Luoyang 470000China Luoyang Fine peony Garden(Mangshan Huafeng Horticulture Garden) Luoyang471000China Henan Forestry Vocational College Luoyang 471002China Luoyang Wangcheng Park Luoyang471000China

In order to reveal the differences between different peony varieties and facilities,thousands of tissue culture seedlings of 21 Itoh peony varieties imported from abroad were used as test materials,and the peony domestication cultivation method used in the past was used as a control. The technical measures and mechanisms for the outdoor domestication of seedlings and the survival of field transplantation were studied. The results indicate that the main factors and measures for effectively protecting the outdoor transplanting of tissue culture seedlings include cultivating sound and strong seedlings,transplanting in proper period of spring,and providing good ventilating places and facilities,removing leaves,keeping buds and releasing dormancy before transplantation,planting depth should meet the requirement of exposing to the root neck,changing the pots according to the size of seedlings,the substrate should be loose and permeable,seriously disinfected and sterilized,scientific management,and prevention and control of diseases and insect pests. In addition,the survival mechanism was also analyzed,and the reasons and countermeasures for the successful application of peony tissue culture in China were found. Finally,the differences in survival rates between different varieties and facilities were summarized.

关键词： Tissue culture seedlings of Paeonia suffruticosa Outdoor domestication cultivation Survival rate Survival mechanism

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牡丹瓷与中原陶瓷的发展

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洛阳师范学院学报 2014年第9期33卷 142-144页

作者：贾晓莉 JIA Xiao-li 洛阳牡丹瓷研究院河南洛阳471000 Luoyang peony Porcelain Ceramic Research Institute Luoyang 471000 China

陶瓷作为我国一项伟大发明,历史悠久,凝聚着中华民族智慧的结晶,是中华民族对人类社会文明发展的重大贡献。洛阳作为世界著名文化古都,长期以来是中国古代的政治、经济、文化中心,在中国陶瓷的发展历史上有着举足轻重的地位。在传承中... 详细信息

陶瓷作为我国一项伟大发明,历史悠久,凝聚着中华民族智慧的结晶,是中华民族对人类社会文明发展的重大贡献。洛阳作为世界著名文化古都,长期以来是中国古代的政治、经济、文化中心,在中国陶瓷的发展历史上有着举足轻重的地位。在传承中国古代陶瓷技艺尤其是唐白瓷、唐三彩制作技艺的基础上,依托河洛地区出土和传世且有牡丹纹饰的三彩陶瓷器,形成了一种用手工制作而成的新派艺术陶瓷——牡丹瓷。

关键词：陶瓷洛阳牡丹瓷

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Effect of Combined Chilling and GA_3 Treatment on Bud Abortion in Forced ‘Luoyanghong' Tree peony(Paeonia suffruticosa Andr.)

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Horticultural Plant Journal 2018年第6期4卷 250-256页

作者： MORNYA Philip M.P. CHENG Fangyun National Flower Engineering Research center College of Landscape Architecture Beijing Forestry University School of Natural Resources Management Njala University National Flower Engineering Research center College of Landscape ArchitectureBeijing Forestry University

This research investigated the effect of combined chilling and GA_3 treatment on bud abortion of tree peony under greenhouse conditions to determine the dynamics of exogenous hormones and chilling effects on bud abortion. Research of such nature will increase the understanding of the effects of GA_3 and chilling level on flower development thereby, promoting off-season cultivation techniques in tree peonies to increase production that meets market demand. Hormone and sugar levels in the ‘Luoyanghong' tree peony were respectively determined using High-Performance Liquid Chromatography(HPLC) and spectrophotometer. Non-potted chilling with 200 mg·L^(-1) GA_3 treatment reduced bud abortion rate. Flowering rate in non-potted chilling with 200 mg·L^(-1) GA_3 plants was 58%, as against 21% in potted chilling plants with 200 mg·L^(-1) GA_3 treatment. Sugar accumulation in retained or aborted flower buds was conversely related to ABA level. Low ABA and high sugar levels were noted in retained buds whereas the reverse induced bud abortion. ABA level apparently inhibited sucrose and reducing sugar uptake in aborted buds whereas GA_3 enhanced sucrose uptake in retained buds. GA_3 and IAA levels were higher in retained than in aborted buds, which made plants with retained bud more competitive in nutrient uptake(by root)for growth and development. Overall, combined chilling and GA_3 treatment enhanced plant growth and development, particularly bud sprouting, growth and flowering.

关键词： tree peony bud abortion chilling hormone sugar GA3 ABA IAA

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The Effect of peony and Licorice Decoction on the Voltage-Gated Sodium Channel Subtype 1.4 Based on Standard Decoction

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World Journal of Traditional Chinese Medicine 2018年第2期4卷 69-76页

作者： Guang-Wei Zhu Jie Guo Ya-Jing Li Lu Luo Toru Sugita Takehisa Tomoda Research center for Quality Standard China Academy of Chinese Medicinal SciencesInstitute of Chinese Materia MedicaSchool of Traditional Chinese Materia Medica Research center for Quality Standard Shandong University of Traditional Chinese Medicine QC center of TCM China Academy of Chinese Medicinal SciencesInstitute of Acupuncture and Moxibustion Research center for Quality Standard TSMUURA and Co.

Objective:The objective of this study is to investigate the inhibitory effect of peony and licorice decoction and its compatibility components on the Nav1.4 voltage-gated sodium channels(VGSCs).Materials and Methods:Writhing test was carried out with ICR *** lactiflora and Glycyrrhiza uralensis group were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 2.94 mg/ml,1.47 mg/ml,and 0.74 mg/ml using intragastric administration,*** and licorice decoction groups were administrated 0.2 ml of solution of freeze-dried powder dissolved in normal saline with the concentration of 5.89 mg/ml,2.94 mg/ml,and 1.47 mg/ml using intragastric administration,*** electrophysiology studies,each freeze-dried powder was dissolved in DMSO to make 10 mg/ml and 50 mg/ml stock *** electrophysiological recordings were obtained under visual control of a *** UPLC analysis,the freeze-dried powder was dissolved in methanol and then determines the contents of the nine marker ***:The effect of *** on incubation period and writhing frequency was significantly better than that of peony and licorice decoction group and *** *** inhibition rate of 50 mg/ml water extracts of the three samples was significantly higher than that of the 10 mg/ml ***,the water extract of *** at 50 mg/ml had the strongest inhibitory effect on I_(Nav) 1.4 of the ***:The possible mechanism of peony and licorice decoction in relieving spasm and pain is most likely by inhibiting Voltage-Gated Sodium Channel Subtype 1.4.

关键词： Nav1.4 voltage-gated sodium channels Paeonia lactiflora and Glycyrrhiza uralensis peony and licorice decoction spasm and pain relieving standard decoction

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Design and test of 2ZLX-2 transplanting machine for oil peony

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International Journal of Agricultural and Biological Engineering 2020年第4期13卷 61-69页

作者： Jiangtao Ji Qun Cheng Xin Jin Zhihong Zhang Xiaolin Xie Mingyong Li College of Agricultural Equipment Engineering Henan University of Science and TechnologyLuoyangHenan 471003China Collaborative Innovation center of Machinery Equipment Advanced Manufacturing of Henan Province LuoyangHenan 471003China

In order to improve the quality of oil peony transplanting,based on the characteristics of peony seedlings and the transplanting effect,the transplanting process was analyzed.A peony seedling transplanting machine was designed,which was mainly composed of a chain-clamp type planting mechanism(clamp,transmission chain,sprocket),slideway,profiling wheels,trencher,the power transmission system and *** the movement analysis of the planting mechanism and its operation process,its structural parameters were *** effective length of the clamp was determined as L_(1)=235 mm,the adjustment range was 235-285 mm,and the height of the planting trajectory was H=340 *** on the physical characteristics and mechanical characteristics of the peony seedlings,the parameters of the slideway and the putter were *** effective length of the slideway was determined as L_(2)=420 cm,the width T_(2)=105 mm,the distance between the slideways S=45 *** initial angle difference of the putter wasθ=13°,and the putter rotation angleβ=37°,putter height ratioε=*** on the above parameters and design requirements,the radius of the planting sprocket R_(1)=42 mm,R_(2)=80 mm.“Feng Dan”peony seedlings were used as transplantation test *** results showed that:when the planting frequency was 60-75 plants/min,the upright rate was 89.2%-92.8%,the leakage rate was 0-0.28%,the injury rate of seedlings was 0,and the qualified rate of planting depth was 91.7%-95.3%,the variation coefficient of plant spacing was 0.25%-0.54%.This machine can effectively improve the quality and efficiency of transplanting,meeting the requirements of mechanical design and production needs.

关键词： peony transplanting planting mechanism oil peony peony seedlings parameter design test analysis

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peony pollen derived nitrogen-doped activated carbon for supercapacitor application

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Chinese Chemical Letters 2020年第6期31卷 1644-1647页

作者： Yiming Liu Zhongxun An Mingxia Wu Anbao Yuan Hongbin Zhao Jiujun Zhang Jiaqiang Xu NEST Lab Department of PhysicsDepartment of ChemistryCollege of SciencesShanghai UniversityShanghai 200444China National Engineering Research center for Supercapacitor for Vechicles Shanghai AOWEI Technology Development Co.Ltd.Shanghai 201203China

peony pollen is a cheap and readily available biomass material with a relatively high protein *** this work,it was employed as an N-rich precursor to prepare the nitrogen-doped porous carbon for supercapacitor *** porous carbon microspheres were prepared through a hydrothermal method and subsequent carbonization ***,ammonium borofruoride and potassium hydroxide were employed respectively as an etchant and an activator to modify the porosity of the *** as prepared ANPPCs-700 has a super high BET specific surface area of 824.69 m^2/*** microstructure,chemical state and electrochemical properties of the product were investigated in *** prepared nitrogen-doped carbon microspheres exhibits excellent specific capacity of 209 F/g at a current density of lA/g and remained 92.5%of the initial capacitance after 5000 deep cycles at 5 A/g.

关键词： Supercapacitors Biomass Nitrogen-doped Hierarchical carbon peony pollen

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Light shading improves the yield and quality of seed in oil-seed peony(Paeonia ostii Feng Dan)

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Journal of Integrative Agriculture 2018年第7期17卷 1631-1640页

作者： HAN Chen-jing WANG Qi ZHANG Hong-bao WANG Shou-hai SONG Hua-dong HAO Jian-mei DONG He-zhong Cotton Research center Shandong Academy of Agricultural SciencesJinan 250100P.R.China Chengwu Agricultural Bureau Heze 274200P.R.China Shuangquan Town Extension Station of Agro-Technology Jinan 250303P.R.China

Tree peony seed is unique for its super-high content of unsaturated fatty acid and is thus considered as an important source of woody oil. However, photosynthetic production is greatly reduced under high light intensity and air temperature during the seed filling period, which negatively affects seed yield and quality. The objective of this study was to determine if appropriate shading improves yield and quality of seed in oilseed peony. In this study, oilseed peony trees were shaded by different density polyethylene nets from four weeks after flowering to harvest stages to form light, moderate, and severe shadings, equivalent to about 80, 40, and 20% of full solar exposure, respectively. The effects of different shadings on some physiological parameters, yield and yield components, and nutritional composition of seed were examined. Averaged across two years, light shading increased the actual net photosynthetic rate（P_n） by 16.8%, the maximum net photosynthetic rate（P_（max）） by 81.4%, chlorophyll（Chl） content by 52.8%, auxin（IAA） content by 38.1%, and gibberellic acid（GA_3） content in leaves by 6.3%; it decreased the accumulation of H_2O_2 in leaves by 24.8%, malondialdehyde（MDA） by 22%, and endogenous abscisic acid（ABA） by 8.8%, indicating that leaf senescence in late season was considerably delayed. Light shading increased seed yield, and contents of crude fat and unsaturated fatty acids by 9.7, 5.6, and 9.6%, respectively, while moderate or severe shading significantly reduced all the three parameters. Light shading increased seed weight, but moderate or severe shading reduced seed weight or follicle density. The improved seed yield under light shading was mainly due to increased seed weight, while the reduced seed yield under moderate or severe shading was mainly attributed to reduce follicle density and seed weight. The improved seed weight and content of unsaturated fatty acids under light shading was possibly due to the delayed leaf senescence. The overall r

关键词： oilseed peony shading yield nutritional compositions physiological responses

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Comparative advantages of chemical compositions of specific edible vegetable oils

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Oil Crop Science 2023年第1期8卷 1-6页

作者： Li Xue Ruinan Yang Xuefang Wang Fei Ma Li Yu Liangxiao Zhang Peiwu Li Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsLaboratory of Risk Assessment for Oilseed Products(Wuhan)Quality Inspection and Test Center for Oilseed ProductsOil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan430062China College of Food Science and Engineering Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and SafetyNanjing210023China Hubei Hongshan Laboratory Wuhan430070China Xianghu Laboratory Hangzhou311231China

As important supplementary to major edible oils, comparative chemical advantages of minor edible oils decidetheir development and usage. In this study, chemical composition of 13 kinds of specific edible vegetable oilswere investigated. The comparative advantages of chemical compositions of these edible oils were obtained asfollows: (1) camellia, tiger nut and almond oil were rich in oleic acid, the contents of which accounted for79.43%, 69.16% and 66.26%, respectively;(2) safflower oil contained the highest content of linoleic acid(76.69%), followed by grape seed (66.85%) and walnut oil (57.30%);(3) perilla seed, siritch, peony seed andherbaceous peony seed oil were rich in α-linolenic acid (59.61%, 43.74%, 40.83% and 30.84%, respectively);(4)the total phytosterol contents of these oils ranged from 91.46 mg/100 g (camellia oil) to 506.46 mg/100 g (siritchoil);and (5) The best source of tocopherols was sacha inchi oil (122.74 mg/100 g), followed by perilla seed oil(55.89 mg/100 g), peony seed oil (53.73 mg/100 g) and herbaceous peony seed oil (47.17 mg/100 g). Thecomparative advantages of these specific edible oils indicated that they possess the high potential nutritionalvalues and health care functions.

关键词： Comparative advantages Fatty acid composition Phytosterols Tocopherols Specific vegetable oils Chemical composition

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