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Food Science and Human Wellness 2024年第2期13卷 698-708页

作者： Hongyun Zhang Guangqiang Wang Zhiqiang Xiong Zhuan Liao Yangyan Qian Xin Song Li Sui Lianzhong Ai Yongjun Xia Shanghai Engineering research center of Food microbiology School of Health Science and EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China Department of Gastroenterol Digestive Endoscopy CenterShanghai HospitalShanghai 200093China

Probiotics have great potential in regulating intestinal *** this study,the effects of Lactobacillus plantarum AR495 on the visceral sensitivity and gut microbiota of irritable bowel syndrome(IBS)rats were *** results showed that tryptase released after mast cell activation and degranulation plays a key role in visceral pain,and *** AR495 reduced the stimulation of colonic mast cells and the expression of protease-activated receptor 2(PAR2)and TRPV1 in dorsal root *** further showed that supplementation with *** AR495 increased the level of short-chain fatty acids(SCFAs)and enhanced the barrier function of the *** addition,the microbiota analysis of the colon indicated that *** AR495 promoted the proliferation of Bifidobacterium and inhibited the proliferation of Lachnospiraceae,which alleviated the imbalance of the intestinal microbiota caused by IBS to a certain *** total,*** AR495 might reduce visceral sensitivity through the Mast cell-PAR2-TRPV1 signaling pathway by maintaining the homeostasis of the intestinal barrier.

关键词： Lactobacillus plantarum Irritable bowel syndrome Visceral sensitivity Dorsal root ganglia Tryptase Microbiota

Gut microbial interactions based on network construction and bacterial pairwise cultivation

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Science China(Life Sciences) 2024年第8期67卷 1751-1762页

作者： Min-Zhi Jiang Chang Liu Chang Xu He Jiang Yulin Wang Shuang-Jiang Liu State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266000China State Key Laboratory of Microbial Resources and Environmental Microbiology Research Center(EMRC)Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Association networks are widely applied for the prediction of bacterial interactions in studies of human gut ***,the experimental validation of the predicted interactions is challenging due to the complexity of gut microbiomes and the limited number of cultivated *** this study,we addressed this challenge by integrating in vitro time series network(TSN)associations and cocultivation of TSN taxon *** samples were collected and used for cultivation and enrichment of gut microbiome on YCFA agar plates for 13 *** cells were harvested for DNA extraction and metagenomic sequencing.A total of 198 metagenome-assembled genomes(MAGs)were *** dynamics of bacteria growing on the YCFA agar were used to infer microbial association *** experimentally validate the interactions of taxon pairs in networks,we selected 24 and 19 bacterial strains from this study and from the previously established human gut microbial biobank,respectively,for pairwise *** co-culture experiments revealed that most of the interactions between taxa in networks were identified as neutralism(51.67%),followed by commensalism(21.67%),amensalism(18.33%),competition(5%)and exploitation(3.33%).Genome-centric analysis further revealed that the commensal gut bacteria(helpers and beneficiaries)might interact with each other via the exchanges of amino acids with high biosynthetic costs,short-chain fatty acids,and/or *** also validated 12 beneficiaries by adding 16 additives into the basic YCFA medium and found that the growth of 66.7%of these strains was significantly *** approach provides new insights into the gut microbiome complexity and microbial interactions in association *** work highlights that the positive relationships in gut microbial communities tend to be overestimated,and that amino acids,short-chain fatty acids,and vitamins are contributed to the positive relationships.

关键词： human gut microbiome gut microbial pairwise interaction co-culture association network microbial community

Virus-like nanoparticle vaccines for inducing long-lasting immunity against infectious diseases

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National Science Review 2024年第4期11卷 13-15页

作者： Yi-Nan Zhang Sarah Auclair Jiang Zhu Department of Integrative Structural and Computational Biology The Scripps Research Institute Department of Immunology and microbiology The Scripps Research Institute

Prophylactic vaccines that can generate long-lasting protective immune responses are needed for many infectious diseases. Efficient vaccination requires vaccine antigens to be retained and presented to B cells in lymp...

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Microbe-induced gene silencing explores interspecies RNAi and opens up possibilities of crop protection

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Science China(Life Sciences) 2024年第3期67卷 626-628页

作者： Rongxiang Fang State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciencesand National Plant Gene Research CenterBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

In most eukaryotes, small RNA(sRNA)-mediated RNA silencing(or RNA interference, RNAi) is an evolutionarily conserved mechanism that regulates gene expression in a sequence-specific ***, s RNAs are produced by the RNase III enzymes Dicers and then incorporated into Argonaute(AGO) proteins to regulate gene expression by mRNA degradation.

关键词： protection conserved manner

4-Hydroxy-2-Oxoglutaric Acid,A KeyMetabolite Involved in Trypsin-Regulation of Arginine Metabolism in Hylocereus undatus during Storage

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Phyton-International Journal of Experimental Botany 2024年第5期93卷 885-900页

作者： Bairu Li Jingyu Jia Hemin Wang Jiaju Sun Enyan Chen Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Henan Engineering research center of Food microbiology Luoyang471023China National Demonstration center for Experimental Food Processing and Safety Education Luoyang471000China

Trypsin,a novel superoxide scavenger,significantly enhances the storage quality of Hylocereus undatus(***).To elucidate the preservation mechanism of trypsin on ***,a widely targeted metabolomic analysis,and transcriptomics analysis were ***,a total of 453 metabolites were identified,with organic acids and their derivatives constituting the largest proportion(25%).Amino acids and their metabolites,prominent among organic acids,were further *** them,73 metabolites were associated with amino acids,and 37 exhibited significant *** most enriched Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway was arginine biosynthesis(map00220),with polyamine metabolites showing the most pronounced differences,particularly spermine(FC=1.7594).Compared with the control group,4-hydroxy-2-oxoglutaric acid was significantly upregulated(FC=2.117)in the process of spermine ***,the results of Gene Ontology(GO)and KEGG enrichment analysis of the *** transcriptome profile revealed that trypsin treatment led to 187 differentially expressed genes associated with *** GO and KEGG analyses exhibited significant enrichment in the spermine biosynthetic process(GO:0006597)(map:00220)within the arginine biosynthesis ***,most enzymes and metabolites within the spermine biosynthesis pathway in *** were *** results of the PPI network highlighted that ADC,SPDS,and SAMDC,among others,were pivotal proteins involved in trypsin-regulated arginine metabolism and spermine *** study revealed that trypsin could significantly delay postharvest senescence of *** at room *** effect might be attributed to trypsin triggering the synthesis of 4-hydroxy-2-oxoglutaric acid in the fruit peel,thereby promoting the biosynthesis of spermine and other polyamines.

关键词： Trypsin arginine transcriptomics widely targeted metabolomics

EuSHT Acts as a Hub Gene Involved in the Biosynthesis of 6-Hydroxyluteolin and Quercetin Induced by Salt Stress in Eucommia ulmoides

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Phyton-International Journal of Experimental Botany 2024年第8期93卷 2095-2113页

作者： Fuxin Li Enyan Chen Xinxin Chen Jingyu Jia Hemin Wang Jie Zhang Jianrui Sun Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Henan Engineering research center of Food microbiology Luoyang471023China National Demonstration center for Experimental Food Processing and Safety Education Luoyang471000China

Salt stress inhibits plant growth and affects the biosynthesis of its secondary *** are natural compounds that possess many important biological activities,playing a significant role in the medicinal activity of Eucommia ulmoides(***).To investigate the mechanism by which salt stress affects the biosynthesis of flavonoids in ***,a comprehensive analysis of metabolomics and transcriptomics was *** results indicated that salt stress led to the wilting and darkening of *** leaves,accompanied by a decrease in chlorophyll levels,and significantly induced malondialdehyde(MDA)and relative electrical *** salt stress,most metabolites in the flavonoid biosynthesis pathway of *** were upregulated,indicating that flavonoid biosynthesis is likely the main induced pathway under salt *** them,secondary metabolites such as 6-Hydroxyluteolin and Quercetin are likely key metabolites induced by salt *** correlation analysis of transcriptomics and metabolomics revealed that EuSHT is a hub gene induced by salt stress,promoting the production of flavonoids such as *** co-expression network showed a strong positive correlation between EuSHT and the biosynthesis of 6-Hydroxyluteolin and Quercetin,while it exhibited a negative correlation with Catechin *** branches leading to Luteolin and Dihydroquercetin are likely the main pathways for flavonoid compound biosynthesis in the plant stress response during salt *** results of this study provided a preliminary mechanism of secondary metabolites such as flavonoids in the medicinal plant *** induced by salt stress and provided new theoretical support for discussing the mechanism of plant stress *** also provided useful information for subsequent exploration of resistance genes in ***.

关键词： Eucommia ulmoides ﬂavonoid salt stress transcriptomics widely targeted metabolomics

Lactobacillus plantarum AR113 attenuates liver injury in D-galactose-induced aging mice via the inhibition of oxidative stress and endoplasmic reticulum stress

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Food Science and Human Wellness 2024年第2期13卷 885-897页

作者： Yongjun Xia Yujie Gong Xiangna Lin Yijin Yang Xin Song Guangqiang Wang Zhiqiang Xiong Yangyang Qian Zhuan Liao Hui Zhang Lianzhong Ai School of Health Science and Engineering Shanghai Engineering Research Center of Food MicrobiologyUniversity of Shanghai for Science and TechnologyShanghai 200093China College of Life Sciences Linyi UniversityLinyi 276000China Department of Gastroenterol. Digestive Endoscopy CenterChanghai HospitalShanghai 200082China

Probiotics could effectively eliminate excess reactive oxygen species(ROS)generated during aging or lipid metabolism disorders,but their mechanism is *** major purpose of this study was to investigate the mechanism of Lactiplantibacillus plantarun AR113 alleviating oxidative stress injury in the D-galactose induced aging *** result showed that pretreatment with *** AR113 significantly relieving H_(2)O_(2)induced cytotoxicity in HepG2 cells by maintain cell membrane integrity and increasing antioxidant enzyme *** D-galactose induced aging mice,*** AR113 could significantly attenuate liver damage and inflammatory infiltration by promoting endogenous glutathione(GSH)synthesis and activating the Nrf2/Keap1 signaling pathway in mice,and increasing the expression of regulated phaseⅡdetoxification enzymes and antioxidant *** analysis shown that gavage of *** AR113 could significantly reduce the expression of G protein-coupled receptor 78(GPR78)and C/EBP homologous protein(CHOP)proteins,and promote the restoration of endoplasmic reticulum(ER)homeostasis,thereby activating cell anti-apoptotic *** results were also confirmed in H_(2)O_(2)-treated HepG2 *** indicated that *** AR113 could inhibit D-galactose-induced liver injury through dual inhibition of ER stress and oxidative *** AR113 have good application potential in anti-aging and alleviating metabolic disorders.

关键词： Lactiplantibacillus plantarun AR113 HepG2 D-galactose Oxidative stress ER stress

Seroprevalence of neutralizing antibodies against HFMD associated enteroviruses among healthy individuals in Shanghai,China,2022

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Virologica Sinica 2024年第4期39卷 694-698页

作者： Jiayu Wang Wencheng Wu Wei Wang Jingyi Zhang Jianwei Xiao Mingyi Cai Jiaying Guo Run Li Shuangli Zhu Wanju Zhang Min Chen Zheng Teng microbiology Department Shanghai Municipal Center for Disease Control and PreventionShanghai200336China microbiology Department Yangpu Municipal Center for Disease Control and PreventionShanghai200090China microbiology Department Jing’an Municipal Center for Disease Control and PreventionShanghai200070China microbiology Department Changning Municipal Center for Disease Control and PreventionShanghai200050China WHO WPRO Regional Polio Reference Laboratory NHC Key Laboratory for BiosafetyNHC Key Laboratory for Medical VirologyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China

Dear Editor,Hand,foot and mouth disease(HFMD)is one of the most common infectious diseases,particularly in the Asia-Pacific *** the past two decades,HFMD rises to prominence for its heavy burden,with over one million cases reported *** 2013,enterovirus A71(EV-A71)and Coxsackievirus A16(CVA16)were the main pathogens leading to HFMD in the mainland of China(Yang et al.,2017).In recent years,non-EV-A71-non-CVA16 other enteroviruses,such as Coxsackievirus A6(CVA6),Coxsackievirus A10(CVA10)and Coxsackievirus A4(CVA4),were frequently reported and replaced EV-A71 and CVA16 becoming the major causative agents of HFMD(Zhou et al.,2021;Wang et al.,2022).

关键词： enterovirus HFMD al.

Role of homologous recombination/recombineering on human adenovirus genome engineering:Not the only but the most competent solution

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Engineering microbiology 2024年第1期4卷 90-107页

作者： Lisa-Marie Dawson Montaha Alshawabkeh Katrin Schröer Fatima Arakrak Anja Ehrhardt Wenli Zhang Virology and microbiology Center for Biomedical Education and Research(ZBAF)School of MedicineFaculty of HealthWitten/Herdecke UniversityStockumer Str.1058453 WittenGermany

Adenoviruses typically cause mild illnesses,but severe diseases may occur primarily in immunodeficient individuals,particularly ***,adenoviruses have garnered significant interest as a versatile tool in gene therapy,tumor treatment,and vaccine vector *** the past two decades,the advent of recombineering,a method based on homologous recombination,has notably enhanced the utility of adenoviral vectors in therapeutic *** review summarizes recent advancements in the use of human adenoviral vectors in medicine and discusses the pivotal role of recombineering in the development of these ***,it highlights the current achievements and potential future impact of therapeutic adenoviral vectors.

关键词： Adenoviral vector Gene therapy Homologous recombination Oncolytic virus Vaccine vector

Tannins from Acacia mangium Bark as Natural Dyes for Textiles:Characteristics and Applications

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Journal of Renewable Materials 2024年第9期12卷 1625-1637页

作者： Maya Ismayati Nissa Nurfajrin Solihat Fifi Melinda Setiawati Wasrin Syafii Yuki Tobimatsu Deni Zulfiana research center for Biomass and Bioproducts Research Organization for Life Sciences and EnvironmentNational Research and Innovation Agency(BRIN)Jl.Raya Bogor KM 46CibinongBogor16911Indonesia Department of Forest Product Technology Faculty of ForestryIPB UniversityBogor16680Indonesia research Institute for Sustainable Humanosphere Kyoto UniversityUjiKyoto611-0011Japan research center for Applied microbiology Research Organization for Life Sciences and EnvironmentNational Research and Innovation Agency(BRIN)Jl.Raya Bogor KM 46CibinongBogor16911Indonesia

Tannins are capable of producing natural dyes with antioxidant and antibacterial propertis,while synthetic dyes are commonly used in the textile industry,causing environmental issues like water *** research aims to utilize waste tannins as natural dyes as an alternative to synthetic *** study examined the effect of the extraction method on tannin properties such as phenolic content,antioxidants,and antibacterial *** addition,Pyrolysis Gas Chromatography‒Mass Spectrometry(Py-GCMS)analysis was used to identify the effect of extraction temperature on the chemical elucidation of *** effect of tannin concentration was evaluated against four bacteria that are usually found on human skin:Staphylococcus epidermidis,Bacillus subtilis,Propionibacterium acnes,and Staphylococcus *** temperature significantly influences the chemical composition of tannin,which leads to different antioxidant *** maximum antibacterial properties of tannin were obtained at 90℃with the inhibition zone in the range of 0.9–1.0 mm against four bacteria,tannin yield of 26.59%,Gallic Acid Equivalents or total phenolic content(GAE)of 40.30 mg/g,and Radical Scavenging Activity or antioxidant activity(RSA)of 89.88%.Moreover,the concentration of tannin was significantly linear with its antibacterial *** was successfully applied to the textile by using alum as a mordanting agent to create an antibacterial *** textile’s bacterial structure damage was analyzed under Field Emission Scanning Electron Microscopy(FESEM).After 50 washings,tannin-textiles with alum-modified properties remained stable compared to those without alum,with *** and *** being the most vulnerable bacteria,as confirmed by FESEM ***,tannin is a feasible alternative to harmful and nondegradable synthetic dyes and antibacterial agents.

关键词： Acacia mangium tannin antibacterial antioxidant different temperature extraction dyes