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Cytotoxicity of lambda-cyhalothrin on the macrophage cell line RAW 264.7

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Journal of environmental Sciences 2010年第3期22卷 428-432页

作者： Quan Zhang, Cui Wang, Liwei Sun, Ling Li, Meirong Zhao Research center of environmental Science, College of biological and environmental Engineering, Zhejiang University of technology, Hangzhou 310032, China. [a] Research center of environmental Science College of Biological environmental Engineering Zhejiang University of Technology Hangzhou 310032 China

The wide use and wide-spectrum toxicity of synthetic pyrethroids （SPs） insecticides make them an emerging ecotoxicological concern. Some previous studies showed that SPs possessed cytotoxicity in some immune cells such as human lymphocytes and rat bone marrow. However, the cytotoxicity of SPs to macrophages, which are crucial to innate immunity, has not been explored. In the present report, we investigated a new pyrethroid insecticide, lambda-cyhalothrin （LCT）, which may increase the generation of reactive oxygen species （ROS） and DNA damage levels and cause cytotoxicity in RAW 264.7 cells in dose- and time-dependent manners. The results for the first time implicated increased endogenous ROS and DNA damage as co-mediators of LCT-induced cytotoxicity in macrophages. Our results also suggested that macrophages were involved in synthetic pyrethroid-induced adverse immune effects. Considering the ubiquitous environmental presence of SPs, this study provided new information relative to the potential long-term physiological and immunological effects associated with chronic exposure to SPs. Hence, the potential immunotoxicity of SPs should be considered in assessing the safety of these compounds in sensitive environmental compartments.

关键词： lambda-cyhalothrin macrophage reactive oxygen species cytotoxicity

Synthesis,insecticidal and acaricidal activities of novel 2-arylpyrroles

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Science China Chemistry 2013年第1期56卷 117-123页

作者： LIU AiPing TANG Ming YU ShuYing LIU XingPing WANG XiaoGuang PEI Hui WU DaoXin HU Li OU XiaoMing HUANG MingZhi National Engineering Research center for agrochemicals Hunan Research Institute of Chemical Industry Hunan Province Key Laboratory for agrochemicals School of Chemistry and biological Engineering Changsha University of Science and Technology

To search for novel 2-arylpyrroles with unique biological activities,a series of novel 2-arylpyrrole derivatives were designed and synthesized,and their structures were characterized by 1 H and 13 C NMR spectroscopy,MS spectrometry,and elemental *** insecticidal activities against Lepidopteran pests (*** separata) and acaricidal activities against mites (*** urticae) were *** results of bioassays indicate that some of these title compounds exhibited excellent insecticidal and acaricidal *** example,4-bromo-1-((chloromethoxy)methyl)-2-(4-chloro phenyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile (6a),4-bromo-2-(4-chlorophenyl)-1-((2-fluoroethoxy)-methyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile (6d) showed insecticidal activity against Mythimna separata and 4-bromo-2-(4-chlorophenyl)1-((isopropoxymethoxy)methyl)-5-(trifluoro methyl)pyrrole-3-carbonitrile (7d) showed acaricidal activity against Tetranychus *** were more effective than Chlorfenapyr,which has been the only commercialized member of a new class of chemicals of 2-arylpyrroles.

关键词： 2-arylpyrroles Chlorfenapyr synthesis insecticidal activity acaricidal activity

[Current advances in the analysis of free RNA modified nucleosides by high performance liquid chromatography-tandem mass spectrometry]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 3-12页

作者： Lyu-Ye Zhang Wei-Bing Zhang Hai-Lin Wang State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China. Shanghai Key Laboratory of Functional Materials Chemistry School of Chemistry & Molecular Engineering East China University of Science and Technology Shanghai 200237 China. State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China 3. University of Chinese Academy of Sciences Beijing 100049 China. University of Chinese Academy of Sciences Beijing 100049 China.

Post-transcriptional ribonucleic acid (RNA) modifications play crucial roles in regulating gene expression, with both eukaryotic and prokaryotic RNA exhibiting more than 170 distinct and ubiquitous modifications. RNA turnover generates numerous free nucleosides, including unmodified nucleosides and a variety of modified ones. Unlike unmodified nucleosides, modified nucleosides are not further degraded or used in the salvage-synthesis pathway owing to a lack of specific enzymes, which leads to the cytosolic accumulation or cellular efflux of modified nucleosides. These modified nucleosides can act as signaling molecules that regulate downstream pathways once transported to the extracellular space; alternatively, they are metabolized in the bloodstream and excreted in urine. Metabolized modified nucleosides are altered by cellular stress responses and mediate abnormal physiological states. Changes in the urinary and blood levels of modified nucleosides associated with cancer can serve as biomarkers for disease. Therefore, identifying and accurately quantifying nucleosides is vital for understanding RNA degradation and associated patterns of nucleoside metabolism. Such analyses are helpful when studying the biological functions and potential clinical applications of modified nucleosides. In this regard, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) offers significant advantages in terms of sensitivity, selectivity, and efficiency, and has been widely used to analyze DNA and RNA nucleosides/nucleotides and their analogues. Multiple MS detection patterns and quantification methods have been established to detect nucleosides in biological samples, including cultured cells, urine, blood, and tissue samples. However, the development of an accurate HPLC-MS/MS method faces several challenges. Firstly, the presence of a complex biological matrix that contains macromolecules, small molecules, and salts can interfere with analysis. Salts and co-elu

关键词： RNA modifications high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) metabolite analysis methylated nucleosides

Degradation of dichloromethane by an isolated strain Pandoraea pnomenusa and its performance in a biotrickling filter

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Journal of environmental Sciences 2014年第5期26卷 1108-1117页

作者： Jianming Yu Wenji Cai Zhuowei Cheng Jianmeng Chen Collaborative Innovation center of Yangtze River Delta Region Green Pharmaceuticals Zhejiang University of Technology College of biological and environmental Engineering Zhejiang University of Technology

A strain Pandoraea pnomenusa LX-1 that uses dichloromethane （DCM） as sole carbon and energy source has been isolated and identified in our laboratory. The optimum aerobic biodegradation of DCM in batch culture was evaluated by response surface methodology. Maximum biodegradation （5.35 mg/（***）） was achieved under cultivation at 32.8℃, pH 7.3, and 0.66% NaC1. The growth and biodegradation processes were well fitted by Haldane＇s kinetic model, yielding maximum specific growth and degradation rates of 0.133 hr^-1 and 0.856 hr^-1, respectively. The microorganism efficiently degraded a mixture of DCM and coexisting components （benzene, toluene and chlorobenzene）. The carbon recovery （52.80%-94.59%） indicated that the targets were predominantly mineralized and incorporated into cell materials. Electron acceptors increased the DCM biodegradation rate in the following order： mixed 〉 oxygen 〉 iron 〉 sulfate 〉 nitrate. The highest dechlorination rate was 0.365 mg C1-/（*** biomass）, obtained in the presence of mixed electron acceptors. Removal was achieved in a continuous biotrickling filter at 56%-85% efficiency, with a mineralization rate of 75.2%. Molecular biology techniques revealed the predominant strain as P. pnomenusa LX-1. These results clearly demonstrated the effectiveness of strain LX-1 in treating DCM-containing industrial effluents. As such, the strain is a strong candidate for remediation of DCM coexisting with other organic compounds.

关键词： dichloromethane Pandoraea pnomenusa biodegradation characteristics removal performance carbon balance

Synthesis and Crystal Structure of N-((6-chloropyridin-3-yl)methyl)-6-ethoxy-N-ethyl-3-nitropyridin-2-amine

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Chinese Journal of Structural Chemistry 2012年第5期31卷 621-624页

作者：吴道新刘民华柳爱平黄明智胡志彬任叶果刘兴平唐明左文清 School of Chemistry and biological Engineering Changsha University of Science and Technology National Engineering Research center for agrochemicals Hunan Research Institute of Chemical Industry College of Chemical Engineering Xiangtan University

The title compound N-（（6-chloropyridin-3-yl）methyl）-6-ethoxy-N-ethyl-3-nitropyri-din-2-amine（4） was synthesized by reacting the mixture of 6-chloro-2-ethoxy-3-nitropyridine（1） and 2-chloro-6-ethoxy-3-nitropyridine（2） with 3,and its structure was determined by X-ray single-crystal *** crystal belongs to the monoclinic system,space group P21/n with a = 7.3441（2）,b = 9.9041（3）,c = 11.7368（3） ,α = 101.410（2）,β = 102.1340（10）,γ = 99.594（2）o,μ = 0.260 mm^-1,Mr = 336.78,V = 798.58（4）（A°）^3,Z = 2,Dc = 1.401g/cm^3,F（000） = 352,T = 296（2） K,R = 0.0210 and wR = 0.1053.

关键词： synthesis crystal structure N-((6-chloropyridin-3-yl)methyl)-6-ethoxy-N-ethyl-3-nitropyridin-2-amine insecticidal and fungicidal activities

Reduced Graphene Oxide Mediated SnO_2 Nanocrystals for Enhanced Gas-sensing Properties

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Journal of Materials Science & technology 2013年第2期29卷 157-160页

作者： Yanhong Chang Yunfeng Yao Bin Wang Hui Luo Tianyi Li Linjie Zhi School of Civil and environmental Engineering University of Science and Technology Beijing National center for Nanoscience and technology School of Chemical and biological Engineering University of Science and Technology Beijing

SnO2-reduced graphene oxide （SnO2-rGO） composites were prepared via a hydro-thermal reaction of graphene oxide （GO） and SnCI2·2H2O in the mixed solvent of ethylene glycol and water. During the redox reaction, GO was reduced to rGO while Sn2＋ was oxidized to Sn02, uniformly depositing on the surface of rGO sheets. The composites were characterized by X-ray diffraction （XRD）, scanning electron microscopy （SEM）, thermogravimetric analysis （TGA）, infrared spectra analysis （IR） and transmission electron microscopy （TEM）, respectively, and their gas sensing properties were further investigated. Compared with pure SnO2 nanoparticles, the as-prepared SnO2-rGO gas sensor and response-recovery time to ethanol and H2S at responding and low cost SnO2-rGO gas sensor could showed much better gas sensing behavior in sensitivity ow concentrations. Overall, the highly sensitive, quick- be potentially applied in environmental monitoring area.

关键词： SnO2-rGO Gas sensor Sensitivity Response-recovery time

Enantioselective induction of oxidative stress by acetofenate in rat PC12 cells

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Journal of environmental Sciences 2010年第12期22卷 1980-1986页

作者： Ling Li Fen Hu Cui Wang Xuedong Wang Research center of environmental Science College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310032 China School of environmental Science and Public Health Wenzhou Medical College Wenzhou 325035 China

In a non-chiral environment, the enantiomers of a racemate possessed the identical physico-cbemical properties, but in the biological systems they possessed different activities. Considering that the involvement of oxidative damage has been implicated in the toxicities of various pesticides, this study investigated the possibility of enantioselective oxidative stress and cytotoxicity induction by acetofenate （AF） which contains an asymmetrical center on PC12 cells. The results of the cytotoxicity assay indicated that S-（＋）-AF presented more toxic effects than R-（-）-AF and （＋）-AF. It also demonstrated that S-（＋）-AF possessed the strongest effects in induction of reactive oxygen species （ROS） production, decrease in superoxide dismutase （SOD） and catalase （CAT） activities, and increase in malondialdehyde （MDA） level. These results suggested that AF and its enanfiomers could induce enantioselecfive cytotoxicity in PC12 cells mediated by oxidative stress. Therefore, the assessment in environmental safety and new chiral pesticide development should consider enantioselectivity.

关键词： chiral organochlorine pesticides acetofenate enantioselectivity cytotoxicity oxidative stress

Research progress on tin-based anode materials for sodium ion batteries

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Rare Metals 2020年第9期39卷 1005-1018页

作者： Ju-Mei Liang Li-Juan Zhang De-Ge XiLi Jing Kang Beijing Key Laboratory for Green Catalysis and Separation College of Environmental and Energy EngineeringCenter of Excellence for Environmental Safety and Biological EffectsBeijing University of TechnologyBeijing 100124China

Sodium ion batteries(SIBs)is considered as a promising alternative to the widely used lithium ion batteries in view of the abundant resources and uniform distribution of sodium on the ***,due to the lack of suitable anode and cathode materials,especially the anode materials with excellent performance,its practical application is *** recent years,lots of attentions are devoted to developing new electrode materials with high sodium storage capacity and long *** a large number of anode material libraries,tin-based materials with alloying reaction mechanism show great potential for application in high-energy SIBs due to their high theoretical specific *** this paper,detailed and comprehensive research progress on tin-based anodes(including tin metal,tin alloy as well as its compounds)in recent years is *** efforts to improve the electrochemical properties of tin-based anode materials are ***,the challenges and prospects of these anode materials are also proposed in this review.

关键词： Tin-based material Anode Sodium ion battery Electrochemical performance

Estrogenic activities of two synthetic pyrethroids and their metabolites

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Journal of environmental Sciences 2010年第2期22卷 290-296页

作者： Meiqing Jin Ling Li Chao Xu Yuezhong Wen Meirong Zhao MOE Key Laboratory of environmental Remediation and Ecosystem Health College of Environmental and Resource Sciences Zhejiang University Hangzhou 310027 China Research center of environmental Science College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310032 China

Synthetic pyrethroids （SPs） are among the most common pesticides in current use, and so far, several SPs have been assessed for their potential estrogenicities by various methods. Previous studies have shown that the estrogenicities partly come from their metabolites. Although considerable information is available with respect to the metabolism and environmental degradation of SPs, little is known about the estrogenicities of the metabolites. In this study, permethrin （PM） and β-cypermethrin （CP）, as well as their metabolites （3-phenoxybenzoic alcohol （PBCOH）, 3-phenoxybenzaldehyde （PBCHO） and 3-phenoxybenzoic acid （PBCOOH） were evaluated for their estrogenic activities in the MCF-7 human breast carcinoma cell line. In the MCF-7 cell proliferation assay, PM and CP exhibited significant estrogenic activities at 10-7 mol/L, comparable to 17β-estradiol （E2） of 10-9 tool/L, with the relative proliferative effect ratios of 55.4% and 56.3%, respectively. The real-time quantitative polymerase chain reaction （qRT-PCR） results confirmed the estrogenicities of PM and CP with significant alteration of pS2 and ERα mRNA levels observed at 10-6 mol/L. For the three major metabolites, PBCOH and PBCOOH exhibited estrogenic activities in all assays, while no significant estrogenic responses was observed for PBCHO compared to the vehicle control. In particular, PBCOH had even slightly stronger estrogenic activity than its parent compounds, indicating that metabolism may be one of the reasons for the estrogenicities of the SPs. Given the widespread use of SPs, the toxicological effects of parent compounds and their metabolites should be taken into consideration in the risk assessment of SPs.

关键词： pyrethroids metabolites cell proliferation gene expression estrogenic activity