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Chinese Science Bulletin 1999年第4期44卷 348-351页

作者： JIANG Shuye, CHEN Qifeng and FANG Xuanjun biotechnology Research center, Chinese Academy of agricultural Science, Beijing 100081, China Institute of Genetics and Crop Breeding, Fujian agricultural University, Fuzhou 350002, China Corresponding author biotechnology Research center Chinese Academy of Agricultural Science Beijing China Institute of Genetics and Crop Breeding Fujian Agricultural University Fuzhou China

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility *** populations were analyzed using cDNA-RAPD. The results showed that: ( ⅰ ) bulked sampling method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advantage in avoiding false positive caused by conventional sampling method. (ⅱ) Among 150 random primers used, 83 primers amplified the same banding patterns, and 34 primers amplified the same banding pattern but different staining of intensity on gel. ( ⅲ ) 33 primers amplified differential cDNA bands between fertile and sterile cDNA populations, and the ratio of polymorphism was 22%. It is concluded that there may exist a lot of genes relating to sterility, which makes the differentially expressed cDNA fragments complicated.

关键词： photo-period sensitive genie male sterile genes cDNA-RAPD differential display analysis.

Helicobacter pylori neutrophil activating protein as target for new drugs against *** inflammation

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World Journal of Gastroenterology 2011年第21期17卷 2585-2591页

作者： Theodora Choli-Papadopoulou Filippos Kottakis Georgios Papadopoulos Stefanos Pendas Laboratory of biochemistry School of ChemistryAristotle University of Thessaloniki Molecular Oncology Research Institute Tufts Medical Center Department of biochemistry and biotechnology University of Thessaly Department of Pharmacy University of Patras

Helicobacter pylori(***) infection is among the most common human infections and the major risk factor for peptic ulcer disease and gastric ***-in this work we present the implication of C-terminal region of *** neutrophil activating protein in the stimulation of neutrophil activation as well as the evi-dence that the C-terminal region of *** activating protein is indispensable for neutrophil adhesion to endothelial cells,a step necessary to *** *** addition we show that arabino galactan proteins derived from chios mastic gum,the natural resin of the plant Pistacia lentiscus *** inhibit neutrophil activation in vitro.

关键词： Helicobacter pylori neutrophil activating protein Helicobacter pylori Peptic ulcer disease Gastric cancer

Identification of a novel DNA-binding protein to osmotin promoter

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Science China(Life Sciences) 1998年第6期41卷 657-663页

作者：徐平凌建群李德葆 biotechnology Institute Zhejiang Agricultural University Hangzhou China center for Plant Environmental Stress Physiology Purdue University West Lafayette USA

One novel osmotin promoter, binding_protein (OPBP1) gene, was isolated from salt adapted tobacco suspension cells using yeast one hybrid system. The OPBP1 interacted specifically in vivo with FA, a DNA sequence from the 5′ upstream region of osmotin gene, which was essential for osmotin responsiveness. The deduced amino acid sequence of OPBP1 contained a conserved motif of a new gene family, AP2 family. This protein did not contain the typical motif found in the most known DNA binding proteins and transcription factors.

关键词： osmotin DNA binding protein one hybrid screen.

A secretory phospholipase A2 of a fungal pathogen contributes to lipid droplet homeostasis,assimilation of insect-derived lipids,and repression of host immune responses

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Insect Science 2022年第6期29卷 1685-1702页

作者： Juan Deng Zhuoyue Lu Huifang Wang Ning Li Guimei Song Qiankuan Zhu Jingxin Sun Yongjun Zhang biotechnology Research center Academy of Agricultural SciencesSouthwest UniversityChongqingChina College of Plant Protection Southwest UniversityChongqingChina

Secretory phospholipase A2s(sPLA2s)are found in a wide range of organisms from bacteria to higher plants and animals and are involved in varied and cellular ***,roles of these enzymes in microbial pathogens remain ***,an sPLA2(BbPLA2)was characterized in the filamentous insect pathogenic fungus,Beau-veria ***2was exclusively expressed in insect hemolymph-derived cells(hyphal bodies),and its expression was induced by insect-derived nutrients and lipids,and nutrient *** levels of secretion of BbPLA2 were observed as well as its distri-bution in hyphal body lipid drops(LDs).Overexpression of BbPLA2 increased the ability of *** to utilize insect-derived nutrients and lipids,and promoted LD accumula-tion,indicating functions for BbPLA2 in mediating LD homeostasis and assimilation of insect-derived *** overexpressing BbPLA2 showed moderately increased vir-ulence,including more efficient penetration of the insect cuticle and evasion of host im-mune responses as compared to the wild type *** addition,***-activated host immune genes were downregulated in the BbPLA2 overexpression strain,but upregulated by infections with a△BbPLA2 *** data demonstrate that BbPLA2 contributes to LD homeostasis,assimilation of insect-derived lipids,and repression of host immune responses.

关键词： Beauveria bassiana fungal pathogen immune response lipid drop secre-toryphospholipaseA2 virulence

The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

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Frontiers of Environmental Science & Engineering 2021年第3期15卷 87-96页

作者： Min Gao Ziye Yang Yajie Guo Mo Chen Tianlei Qiu Xingbin Sun Xuming Wang Beijing Key Laboratory of agricultural Genetic Resources and biotechnology Beijing Agro-Biotechnology Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Forestry Northeast Forestry UniversityHarbin 150040China

Composting plants are regarded as one of the important sources of environmental ***,limitations in the size distribution of airborne bacteria have prevented our comprehensive understanding of their risk to human health and their dispersal *** this study,different sizes of airborne bacteria were collected using an eight-stage impactor from a full-scale composting ***-related abundance and communities of airborne bacteria as well as human pathogenic bacteria(HPB)were investigated using 16S rRNA gene sequencing coupled with droplet digital *** results indicate that the bacterial concentrations from the eight stages were approximately 10^(4)-105copies/m^(3).Although no statistical correlation was detected between the particle size and the Shannon index,the influence of size on bacterial lineages was observed in both composting and packaging *** airborne bacteria from different stages,the dominant phyla were Firmicutes,Proteobacteria,and Actinobacteria,and the dominant genera was *** out of eight HPB with a small geometric mean aerodynamic diameter had a high concentration in composting *** on diameters of 2.42 to 5.09μm,most HPB in the composting areas were expected to be deposited on the bronchus and secondary ***,in the packaging areas,the deposition of HPB(diameters 3.70 to 8.96μm)occurred in the upper part of the respiratory *** results on the size distribution,abundance,and diversity of these bacteria offer important information for the systematic evaluation of bacterial pathogenicity and the potential health impacts on workers in composting plants and the surrounding residents.

关键词： Size distribution Airborne bacteria Biological diversity Human pathogenic bacteria Composting plants

Degradation of corn stalk by the composite microbial system of MC1

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Journal of Environmental Sciences 2008年第1期20卷 109-114页

作者： GUO Peng WANG Xiaofen ZHU Wanbin YANG Hongyan CHENG Xu CUI Zongjun College of Agronomy and biotechnology China Agricultural University Key Laboratory of Crop Cultivation and Farming System Ministry of Agriculture of China Belting 100094 China center of Biomass Engineering China Agricultural University Beifing 100094 China

The composite microbial system of MC1 was used to degrade corn stalk in order to determine properties of the degraded products as well as bacterial composition of MC1. Results indicated that the pH of the fermentation broth was typical of lignocellulose degradation by MC1, decreasing in the early phase and increasing in later stages of the degradation. The microbial biomass peaked on the day 3 after degradation. The MC1 efficiently degraded the corn stalk by nearly 70% during which its cellulose content decreased by 71.2%, hemicellulose by 76.5% and lignin by 24.6%. The content of water-soluble carbohydrates （WSC） in the fermentation broth increased progressively during the first three days, and decreased thereafter, suggesting an accumulation of WSC in the early phase of the degradation process. Total levels of various volatile products peaked in the third day after degradation, and 7 types of volatile products were detected in the fermentation broth. These were ethanol, acetic acid, 1,2-ethanediol, propanoic acid, butanoic acid, 3- methyl-butanoic acid and glycerine. Six major compounds were quantitatively analysed and the contents of each compound were ethanol （0.584 g/L）, acetic acid （0.735 g/L）, 1,2-ethanediol （0.772 g/L）, propanoic acid （0.026 g/L）, butanoic acid （0.018 g/L） and glycerine （4.203 g/L）. Characterization of bacterial cells collected from the culture solution, based on 16S rDNA PCR-DGGE analysis of DNAs, showed that the composition of bacterial community in MC1 coincided basically with observations from previous studies. This indicated that the structure of MC1 is very stable during degradation of different lignocellulose materials.

关键词： corn stalk cellulose degradation composite microbial system MC1

Coronatine promotes maize water uptake by directly binding to the aquaporin ZmPIP2;5 and enhancing its activity

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Journal of Integrative Plant Biology 2023年第3期65卷 703-720页

作者： Rui He Huiqing Su Xing Wang Zhijie Ren Kun Zhang Tianyu Feng Mingcai Zhang Zhaohu Li Legong Li Junhong Zhuang Zhizhong Gong Yuyi Zhou Liusheng Duan State Key Laboratory of Plant Physiology and biochemistry Engineering Research Center of Plant Growth RegulatorMinistry of Education&College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China College of Plant Protection China Agricultural UniversityBeiing 100193China Key Laboratory of Plant Gene Resources and biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal GovernmentCollege of Life SciencesCapital Normal UniversityBeijing 100048China center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Physiology and biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Water uptake is crucial for crop growth and development and drought stress tolerance. The water channel aquaporins(AQP) play important roles in plant water uptake. Here, we discovered that a jasmonic acid analog, coronatine(COR), enhanced maize(Zea mays) root water uptake capacity under artificial water deficiency conditions. COR treatment induced the expression of the AQP gene Plasma membrane intrinsic protein 2;5(ZmPIP2;5).In vivo and in vitro experiments indicated that COR also directly acts on ZmPIP2;5 to improve water uptake in maize and Xenopus oocytes. The leaf water potential and hydraulic conductivity of roots growing under hyperosmotic conditions were higher in ZmPIP2;5-overexpression lines and lower in the zmpip2;5 knockout mutant, compared to wild-type plants. Based on a comparison between ZmPIP2;5 and other PIP2s, we predicted that COR may bind to the functional site in loop E of ZmPIP2;5. We confirmed this prediction by surface plasmon resonance technology and a microscale thermophoresis assay, and showed that deleting the binding motif greatly reduced COR binding. We identified the N241 residue as the COR-specific binding site, which may activate the channel of the AQP tetramer and increase water transport activity,which may facilitate water uptake under hyperosmotic stress.

关键词： coronatine osmotic stress water uptake capacity ZmPIP2 5

Study on the rapid detection of irradiated rice based on RVA

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Nuclear Science and Techniques 2013年第B12期24卷 58-62页

作者： FU Junjie ZHOU Xinyao FENG Duhua WANG Qing DENG Li Zhejiang institute of Nuclear-agricultural Sciences Hangzhou 310029 China The center of biotechnology of Wuxi and Zhejiang University Wuxi 214105 China

In this paper, the changes of rice-starch's viscosity at different dose irradiation treatment were studied, the rice can be easily distinguished whether it has been irradiated or not with the control of pH. The results showed that the starch's viscosity was significantly declined with increasing irradiation absorb dose. With the control of pH 2.5, the irradiated starch's viscosity was apparently higher than the un-irradiated ones, there are significant differences in terms of viscosity between the two parts. The rice absorbed dose can be estimated using the relationship among peak viscosity, hot pasting viscosity and absorbed dose. © 2013, Science Press. All rights reserved.

关键词：辐照处理快速检测水稻 RVA 吸收剂量大米淀粉高粘度 pH值

Dissecting conserved cis-regulatory modules of Glu-1 promoters which confer the highly active endosperm-specific expression via stable wheat transformation

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The Crop Journal 2019年第1期7卷 8-18页

作者： Jihu Li Ke Wang Genying Li Yulian Li Yong Zhang Zhiyong Liu Xingguo Ye Xianchun Xia Zhonghu He Shuanghe Cao National Wheat Improvement center Institute of Crop Sciences Chinese Academy of Agricultural Sciences College of Agronomy and biotechnology China Agricultural University Crop Research Institute Shandong Academy of Agricultural Sciences International Maize and Wheat Improvement center (CIMMYT) China Office c/o CAAS

Wheat high-molecular-weight glutenin subunits(HMW-GS) determine dough elasticity and play an essential role in processing quality. HMW-GS are encoded by Glu-1 genes and controlled primarily at transcriptional level, implemented through the interactions between cis-acting elements and trans-acting factors. However, transcriptional mechanism of Glu-1 genes remains elusive. Here we made a comprehensive analysis of cis-regulatory elements within 1-kb upstream of the Glu-1 start codon(-1000 to-1) and identified 30 conserved motifs. Based on motif distribution pattern, three conserved cis-regulatory modules(CCRMs), CCRM1(-300 to-101), CCRM2(-650 to-400), and CCRM3(-950 to-750), were defined, and their functions were characterized in wheat stable transgenic lines transformed with progressive 5′ deletion promoter::GUS fusion constructs. GUS staining, qP CR and enzyme activity assays indicated that CCRM2 and CCRM3 could enhance the expression level of Glu-1, whereas the 300-bp promoter(-300 to-1), spanning CCRM1 and core region(-100 to-1), was enough to ensure accurate Glu-1 initiation at 7 days after flowering(DAF) and shape its spatiotemporal expression pattern during seed development. Further transgenic assays demonstrated that CCRM1-2(-300 to-209) containing Complete HMW Enhancer(-246 to-209) was important for expression level but had no effect on expression specificity in the endosperm. In contrast, CCRM1-1(-208 to-101) was critical for both expression specificity and level of Glu-1. Our findings not only provide new insights to uncover Glu-1 transcription regulatory machinery but also lay foundations for modifying Glu-1 expression.

关键词： Conserved cis-regulatory modules Glu-1 Transcriptional regulation Transgenic wheat Triticum aestivum

Long noncoding RNAs:new insights in modulating mammalian spermatogenesis

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Journal of Animal Science and biotechnology 2020年第2期11卷 291-302页

作者： Bahlibi Weldegebriall Sahlu Shanjiang Zhao Xiuge Wang Saqib Umer Huiying Zou Jinming Huang Huabin Zhu Embryo biotechnology and Reproduction Laboratory Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193People’s Republic of China Tigray agricultural Research Institute Mekelle Agricultural Research CenterMekelleEthiopia Dairy Cattle Research center Shandong Academy of Agricultural SciencesJinan 250131People’s Republic of China

Spermatogenesis is a complex differentiating developmental process in which undifferentiated spermatogonial germ cells differentiate into spermatocytes,spermatids,and finally,to mature *** multistage developmental process of spermatogenesis involves the expression of many male germ cell-specific long noncoding RNAs(lncRNAs)and highly regulated and specific gene *** are a recently discovered large class of noncoding cellular transcripts that are still relatively *** a few of them have postmeiotic;however,lncRNAs are involved in many cellular biological *** expression of lncRNAs is biologically relevant in the highly dynamic and complex program of spermatogenesis and has become a research focus in recent genome *** review considers the important roles and novel regulatory functions whereby lncRNAs modulate mammalian spermatogenesis.

关键词： Germ cell Long noncoding RNA Mammalian spermatogenesis Regulatory pathways Spermatocytes