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Mesenchymal stem cells loaded on 3D-printed gradient poly(e-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering

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regenerative Biomaterials 2021年第3期8卷 70-83页

作者： Yanyan Cao Peng Cheng Shengbo Sang Chuan Xiang Yang An Xiaochun Wei Zhizhong Shen Yixia Zhang Pengcui Li Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education MicroNano System Research CenterCollege of Information and ComputerTaiyuan University of TechnologyTaiyuan 030024China College of Information Science and Engineering Hebei North UniversityZhangjiakou 075000China Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair Department of OrthopedicsThe Second Hospital of Shanxi Medical UniversityTaiyuan 030001China Department of Plastic Surgery Peking University Third HospitalBeijing 100191China Department of Biomedical Engineering College of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan 030024China

Cartilage has limited self-repair ability due to its avascular,alymphatic and aneural *** combination of three-dimensional(3D)printing and tissue engineering provides an up-and-coming approach to address this ***,we designed and fabricated a tri-layered(superficial layer(SL),middle layer(ML)and deep layer(DL))stratified scaffold,inspired by the architecture of collagen fibers in native cartilage *** scaffold was composed of 3D printed depth-dependent gradient poly(e-caprolactone)(PCL)impregnated with methacrylated alginate(ALMA),and its morphological analysis and mechanical properties were *** prove the feasibility of the composite scaffolds for cartilage regeneration,the viability,proliferation,collagen deposition and chondrogenic differentiation of embedded rat bone marrow mesenchymal stem cells(BMSCs)in the scaffolds were assessed by Live/dead assay,CCK-8,DNA content,cell morphology,immunofluorescence and real-time reverse transcription polymerase chain ***-loaded gradient PCL/ALMA scaffolds showed excellent cell survival,cell proliferation,cell morphology,collagen II deposition and hopeful chondrogenic differentiation compared with three individual-layer ***,our study demonstrates the potential use of the gradient PCL/ALMA construct for enhanced cartilage tissue engineering.

关键词： three-dimensional printing cartilage tissue engineering PCL methacrylated alginate bone marrow mesenchymal stem cells

Lentiviral Gene Therapy for Artemis-Deficient SCID

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四川生理科学杂志 2022年第12期44卷 2107-2107页

作者： Morton J Cowan From the Departments of Pediatrics (M.J.C. J.Y. J.F. C.F.-B. U.S. M.K. J.D. J.L.-B. W.C. S.C. R.C. C.C.D. J.M.P.) and Epidemiology and Biostatistics (J.F.H.) the Smith Cardiovascular Research Institute (M.J.C. J.M.P.) and the School of Pharmacy (J.L.-B.) University of California San Francisco (UCSF) and UCSF Benioff Children's Hospital (M.J.C. J.F. J.D. J.L.-B. J.O. C.C.D. J.M.P.) San Francisco the Department of Pediatrics University of California San Diego and Rady Children's Hospital San Diego (L.B.) and the Department of Pediatrics UCLA Mattel Children's Hospital Los Angeles (C.Y.K.) - all in California the Department of Pediatrics Johns Hopkins All Children's Hospital St. Petersburg FL (D.C.) the Department of Pediatrics Sainte-Justine University Hospital Center University of Montreal Montreal (H.D.) Tuba City Regional Health Care Tuba City (C.G. D.H.) and Phoenix Children's Hospital Phoenix (H.K.M.) - both in Arizona the Department of Pediatrics University of Washington Seattle Children's Hospital Seattle (A.P.) Clinical Development Roche Diagnostics Solutions Singapore (D.P.) the National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD (H.L.M.) and the Department of Genetics Cell Biology and Development University of Minnesota Minneapolis (R.S.M.).

Background:The DNA-repair enzyme Artemis is essential for rearrangement of T-and B-cell *** in DCLRE1C,which encodes Artemis,cause Artemis-deficient severe combined immunodeficiency(ART-SCID),which is poorly responsiv... 详细信息

关键词： SCID immunodeficiency Artemis

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造血干细胞基因治疗：进展与挑战

中国科学：生命科学 2017年第12期47卷 1323-1335页

作者：张健萍程涛张孝兵中国医学科学院&北京协和医学院血液病医院(血液学研究所) 实验血液学国家重点实验室天津300020 中国医学科学院干细胞医学中心天津300020 北京协和医学院干细胞与再生医学系天津300020 肿瘤医学协同创新中心天津300020 Department of medicine Loma Linda UniversityLoma LindaCA 92350USA

造血干细胞具有自我更新和分化为各类血细胞的潜能,一直被认为是最理想的基因治疗靶细胞之一.近年来,基于慢病毒载体的造血干细胞基因治疗逐渐进入临床.同时,随着CRISPR-Cas9等基因编辑技术的不断发展,第二代造血干细胞精准基因治疗研... 详细信息

造血干细胞具有自我更新和分化为各类血细胞的潜能,一直被认为是最理想的基因治疗靶细胞之一.近年来,基于慢病毒载体的造血干细胞基因治疗逐渐进入临床.同时,随着CRISPR-Cas9等基因编辑技术的不断发展,第二代造血干细胞精准基因治疗研究已经取得重要进展,预计将逐渐开始临床转化.但目前,造血干细胞基因编辑还面临一些问题亟待解决,尤其是精准编辑效率还需要大幅度提高.

关键词：造血干细胞基因治疗基因编辑 CRISPR Cas9-sgRNA

Deciphering the potential mechanism of Radix Rehmanniae Praeparata for treating cancer-related anemia based on network pharmacology and molecular docking technology

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TMR Pharmacology Research 2023年第1期3卷 18-25页

作者： Ming-Jie Liu Zhi-Fang Liu Qi Wang Zhong-Yao Li Ming-Wei Sun Mi Xiao Qian Zhou Yuan Li Tao Shen Bao-Bing Zhao Huan-Xin Zhao Ke-Wu Zeng Zhi-Yuan Lu Key Lab of Chemical Biology(MOE) School of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJi’nan 250012China Institute of Leisure Agriculture Shandong Academy of Agricultural SciencesJi’nan 250131China School of Pharmacy and Pharmaceutical Sciences&Institute of Materia Medica Shandong First Medical University&Shandong Academy of Medical SciencesJi’nan 250117China Shandong center For Food and Drug Evaluation&Inspection Ji’nan 250114China China Pharmaceutical Preparation Section Huazhong University of Science and Technology Union Jiangbei Hospital/Wuhan Caidian People’s HospitalWuhan 430100China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China

Background:Rehmanniae Radix Praeparata(RRP,Shu Dihuang in Cinese)is a traditional Chinese herb with multiple pharmacological effects and is commonly used to treat blood deficiency syndrome,such as cancer-related anemia(CRA),alone or in combination with other ***,its main active ingredients and mechanisms of action in treating CRA remain *** study aims to elucidate RRP’s potential mechanism and main active components in treating CRA by using network pharmacology and molecular docking technology ***:The main components of RRP were obtained by the TCMSP database and literature search,and active components and potential targets were obtained by the SwissADME and SwissTargetPridiction *** targets were collected through GeneCards,DisGeNET,and DrugBank ***-protein interaction networks of potential targets were constructed via STRING 11.5 and analyzed visually with Cytoscape *** Metascape platform was used for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis,which were subsequently visualized with Cytoscape 3.9.1 or SangerBox ***,Autodock Vina was used for the molecular docking of potential targets and main active ingredients that were visualized with PyMOL ***:In this study,31 main active ingredients of PPR were screened,and 155 related targets related to CRA were ***-protein interaction results showed that PPR’s core proteins for CRA intervention correlate to STAT3,SRC,MAPK3,MAPK1,PIK3R1,PIK3CA,and *** signaling pathways were closely related to the treatment of CRA intervened by PPR,including the PI3K-Akt signaling pathway,HIF-1 signaling pathway,JAK-STAT3 signaling pathway,TNF-αsignaling,cytokine signaling pathway and NF-kappB signaling pathway,which are closely involved in the proliferation and differentiation of hematopoietic stem cell and inflammatory *** docking results showed that these potential targets had good conformation

关键词： Rehmanniae Radix Praeparata cancer-related anemia network pharmacology molecular docking

Nutritional programming of pancreatic β-cell plasticity

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World Journal of Diabetes 2011年第8期2卷 119-126页

作者： David J Hill Department of medicine Physiology and Pharmacology and Paediatrics University of Western Ontario LondonOntario N6A 5B8 Canada Lawson Health Research Institute St. Joseph's Health Care 268 Grosvenor Street London Ontario N6A 4V2Canada

Nutritional insufficiency during pregnancy has been shown to alter the metabolism of the offspring and can increase the risk of type 2 diabetes. The phenotype in the offspring involves changes to the morphology and functional capacity of the endocrine pancreas, and in the supporting islet microvasculature. Pancreatic β-cells possess a plastic potential and can partially recover from catastrophic loss. This is partly due to the existence of progenitors within the islets and the ability to generate new islets by neogenesis from the pancreatic ducts. This regenerative capacity is induced by bone marrow-derived stem cells, including endothelial cell progenitors and is associated with increased angiogenesis within the islets. Nutritional insults in early life, such as feeding a low protein diet to the mother, impair the regenerative capacity of the β-cells. The mechanisms underlying this include a reduced ability of β-cells to differentiate from the progenitor population, changes in the inductive signals from the microvasculature and an altered presence of endothelial progenitors. Statin treatment within animal models was associated with angiogenesis in the islet microvasculature, improved vascular function and an increase in β-cell mass. This demonstrates that reversal of the impaired β-cell phenotype observed following nutritional insult in early life is potentially possible.

关键词： Islet β-cell Plasticity Diabetes Nutrition Statin

干细胞再生生物学研究悖论及相关法律问题探讨

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医学与社会 2016年第7期29卷 44-46页

作者：杨荣华李忠光崔婧王荣荣王春慧牟玉莲 Hiroshi Takeshima MengMeng Xu 刘万红 William Isaacs 徐学红陕西师范大学生命科学学院西安710119 中国农业科学院北京畜牧兽医研究所北京100193 Kyoto University KyotoJapan MSTP Duke University Medical CenterDurhamNCUSA 武汉大学基础医学院武汉430072 Johns Hopkins University BaltimoreMDUSA

干细胞可分化为构成人体的250余种细胞,这一特性为彻底攻克人类疾病带来了极大的希望,然而,现阶段也存在大量亟待解决的问题。干细胞基础研究结果应用于临床试验还有许多问题没有解决,仍然需要包括动物实验等中间试验在内的数据积累。... 详细信息

干细胞可分化为构成人体的250余种细胞,这一特性为彻底攻克人类疾病带来了极大的希望,然而,现阶段也存在大量亟待解决的问题。干细胞基础研究结果应用于临床试验还有许多问题没有解决,仍然需要包括动物实验等中间试验在内的数据积累。干细胞应用前期的临床试验面临的科研人员职业道德的挑战、科学实验立论不够严谨、急功近利的虚假研究报道等问题必须坚决杜绝,在此基础上才能加快干细胞再生生物学从基础及临床试验走向临床应用的步伐。中国干细胞临床应用相关的法律法规必须借鉴发达国家的经验,组建至少包括法律界资深学者、医学伦理学专家、再生生物学领域的一线科研工作者和政治政策的制定者在内的干细胞应用相关法律委员会,颁布符合国情的法律。

关键词：干细胞再生生物研究悖论法律法规

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研究神经胶质细胞的新兴技术

神经损伤与功能重建 2020年第10期15卷 F0003-F0003页

作者： Hélène Hirbec Nicole Déglon Lynette C Foo Inbal Goshen Jaime Grutzendler Emilie Hangen Tirzah Kreisel Nathalie Linck Julien Muffat Sara Regio Sybille Rion Carole Escartin 杜一星 Institute for Functional Genomics(IGF) University of MontpellierCNRSINSERMMontpellierFrance Laboratory of Neurotherapies and Neuromodulation Department of Clinical NeuroscienceLausanne University HospitalUniversity of LausanneLausanneSwitzerland Laboratory of Neurotherapies and Neuromodulation Neuroscience Research CenterLausanne University Hospital and University of LausanneLausanneSwitzerland Neuroimmunology and Neurodegeneration Section The Neuroscience and Rare Diseases Discovery and Translational AreaF.Hoffman-La RocheBaselSwitzerland Edmond and Lily Safra center for Brain Sciences The Hebrew University of JerusalemJerusalemIsrael Department of Neurology Yale University School of MedicineNew HavenConnecticutUSA Department of Neuroscience Yale University School of MedicineNew HavenConnecticutUSA Commissariatà l'Energie Atomique et aux Energies Alternatives Département de la Recherche FondamentaleInstitut de Biologie François JacobMIRCenFontenay-aux-RosesFrance Centre National de la Recherche Scientifique Neurodegenerative Diseases LaboratoryUniversitéParis-SudUniversitéParis-SaclayUMR 9199Fontenay-aux-RosesFrance Program in Neurosciences and Mental Health The Hospital for Sick Childrenand Department of Molecular GeneticsThe University of TorontoTorontoCanada 不详

大脑的发育、生理功能和病理与神经元和不同类型的神经胶质细胞(如星形胶质细胞、小胶质细胞、少突胶质细胞和少突胶质细胞的前体细胞等)之间的相互作用密切相关。研究脑功能及其功能障碍,除了神经元之外,还需要对不同类型的神经胶质细... 详细信息

大脑的发育、生理功能和病理与神经元和不同类型的神经胶质细胞(如星形胶质细胞、小胶质细胞、少突胶质细胞和少突胶质细胞的前体细胞等)之间的相互作用密切相关。研究脑功能及其功能障碍,除了神经元之外,还需要对不同类型的神经胶质细胞进行研究。近年来,多项技术突破使“神经胶质科学家”能够解决新的具有挑战性的生物学问题。这些技术发展使得以中/高含量工作流研究特定细胞类型的作用成为可能,并在保存完好的环境中对其相互作用进行精细的分析。本综述阐明了几种可能揭示神经胶质细胞在特定脑功能或疾病中作用的尖端实验方法。这些实验方法包括高级细胞培养,诱导多能干细胞(iPSC)衍生的人神经胶质细胞,病毒载体,原位胶质细胞成像,光学遗传学和化学遗传学方法,以及高内涵分子分析。本文还阐述了某些技术的临床转化,通过特定的脑成像方法以监控患者的神经胶质细胞。本文亦讨论了每种技术的优点,缺陷和未来发展,并提供了选定的示例以说明现在如何可以解决特定的“胶质生物学”问题。

关键词：神经胶质细胞少突胶质细胞小胶质细胞星形胶质细胞脑功能细胞培养细胞类型脑成像

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迁移的雪旺细胞指导周围神经桥内的轴突再生

神经损伤与功能重建 2021年第9期16卷 F0003-F0003页

作者： Qing Min David B Parkinson Xin-Peng Dun 杜一星(编译) School of Pharmacy Hubei University of Science and TechnologyXianningHubei ProvincePeople's Republic of China Peninsula Medical School Faculty of HealthPlymouth UniversityPlymouthDevonUK The Co-innovation center of Neuroregeneration Nantong UniversityNantongJiangsu ProvincePeople's Republic of China 不详

周围神经系统内的雪旺细胞具有强大的再生潜力。目前的研究表明,周围神经相关的雪旺细胞具有促进多种组织修复的能力,包括周围神经间隙桥接、皮肤伤口愈合、指尖修复以及牙齿再生。专门修复的雪旺细胞的关键特征之一是它们变得高度能动... 详细信息

周围神经系统内的雪旺细胞具有强大的再生潜力。目前的研究表明,周围神经相关的雪旺细胞具有促进多种组织修复的能力,包括周围神经间隙桥接、皮肤伤口愈合、指尖修复以及牙齿再生。专门修复的雪旺细胞的关键特征之一是它们变得高度能动。它们不仅迁移到受损组织区域,成为组织再生的关键组成部分,还分泌信号分子来吸引巨噬细胞,支持神经元存活,促进轴突再生,激活局部间充质干细胞,并与其他细胞类型相互作用。目前,迁移性雪旺细胞在组织再生中的重要性在周围神经横断损伤的情况证据最为充分。神经横断后,来自近端和远端神经残端的雪旺氏细胞均迁移到神经桥中并形成雪旺细胞索以引导轴突再生。神经桥中雪旺细胞索的形成是横断损伤后周围神经成功修复的关键。本篇综述首先探讨了神经桥的形成以及神经桥中雪旺细胞迁移的行为,然后讨论迁移的雪旺细胞如何将再生轴突引导到远端神经中。我们还回顾了目前对可激活雪旺细胞迁移的信号以及雪旺细胞用于指导轴突再生的信号的理解。了解雪旺细胞迁移的分子机制可能为周围神经修复提供新的治疗策略。

关键词：轴突再生迁移神经桥雪旺细胞信号横断损伤