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Coordinated response of milk bacterial and metabolic profiles to subacute ruminal acidosis in lactating dairy cows

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2483-2495页

作者： Yingyu Mu Wangpan Qi Tao Zhang Jiyou Zhang Shengyong Mao Ruminant nutrition and Feed Engineering Technology research center College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China Laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthNational Center for International Research on Animal Gut NutritionCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Background Bovine milk is an important source of nutrition for human consumption,and its quality is closely associated with the microbiota and metabolites in it.But there is limited knowledge about the milk microbiome and metabolome in cows with subacute ruminal acidosis.Methods Eight ruminally cannulated Holstein cows in mid lactation were selected for a 3-week experiment.The cows were randomly allocated into 2 groups,fed either a conventional diet(CON;40%concentrate;dry matter basis)or a high-concentrate diet(HC;60%concentrate;dry matter basis).Results The results showed that there was a decreased milk fat percentage in the HC group compared to the CON group.The amplicon sequencing results indicated that the alpha diversity indices were not affected by the HC feeding.At the phylum level,the milk bacteria were dominated by Proteobacteria,Actinobacteria,Bacteroidetes,and Firmicutes both in the CON and HC groups.At the genus level,the HC cows displayed an improved proportion of Labrys(P=0.015)compared with the CON cows.Results of both the principal components analysis and partial least squares of discriminant analysis of milk metabolome revealed that samples of the CON and HC groups clustered separately.A total of 31 differential metabolites were identified between the two groups.Of these,the levels of 11 metabolites decreased(α-linolenic acid,prostaglandin E2,L-lactic acid,L-malic acid,3-hydroxysebacic acid,succinyladenosine,guanosine,pyridoxal,L-glutamic acid,hippuric acid,and trigonelline),whereas the levels of the other 20 metabolites increased in the HC group with respect to the CON group(P<0.05).Conclusion These results suggested that subacute ruminal acidosis less impacted the diversity and composition of milk microbiota,but altered the milk metabolic profiles,which led to the decline of the milk quality.

关键词： High-concentrate diet Milk bacteria Milk metabolome Milk quality Subacute ruminal acidosis

Effects of synbiotic supplementation on anthropometric indices and body composition in overweight or obese children and adolescents:a randomized,double-blind,placebo-controlled clinical trial

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World Journal of Pediatrics 2023年第4期19卷 356-365页

作者： Mohammad Amin Atazadegan Motahar Heidari-Beni Mohammad Hassan Entezari Fariborz Sharifianjazi Roya Kelishadi Department of Clinical nutrition School of Nutrition and Food SciencesIsfahan University of Medical SciencesIsfahanIran Department of nutrition Child Growth and Development Research CenterResearch Institute for Primordial Prevention of Non-Communicable DiseaseIsfahan University of Medical SciencesIsfahanIran Department of Clinical nutrition School of Nutrition and Food ScienceIsfahan University of Medical SciencesIsfahanIran School of Science and Technology The University of GeorgiaTbilisiGeorgia Department of Pediatrics Child Growth and Development Research CenterResearch Institute for Primordial Prevention of Non-Communicable DiseaseIsfahan University of Medical SciencesIsfahanIran

Background Recently,beneficial effects of probiotics and/or prebiotics on cardio-metabolic risk factors in adults have been shown.However,existing evidence has not been fully established for pediatric age groups.This study aimed to assess the effect of synbiotic on anthropometric indices and body composition in overweight or obese children and adolescents.Methods This randomized double-blind,placebo-controlled trial was conducted among 60 participants aged 8-18 years with a body mass index(BMI)equal to or higher than the 85th percentile.Participants were randomly divided into two groups that received either a synbiotic capsule containing 6x109 colony forming units(CFU)Lactobacillus coagulans SC-208,6 x 109 CFU Lactobacillus indicus HU36 and fructooligosaccharide as a prebiotic(n=30)or a placebo(n=30)twice a day for eight weeks.Anthropometric indices and body composition were measured at baseline and after the intervention.Results The mean(standard deviation,SD)age was 11.07(2.00)years and 11.23(2.37)years for the placebo and synbiotic groups,respectively(P=0.770).The waist-height ratio(WHtR)decreased significantly at the end of the intervention in comparison with baseline in the synbiotic group(0.54±0.05 vs.0.55±0.05,P=0.05).No significant changes were dem-onstrated in other anthropometric indices or body composition between groups.Conclusions Synbiotic supplementation might be associated with a reduction in WHtR.There were no significant changes in other anthropometric indices or body composition.

关键词： Anthropometry Body composition Pediatrics Synbiotics

Microbiota-host interplay at the gut epithelial level,health and nutrition

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Journal of Animal Science and Biotechnology 2017年第2期8卷 327-334页

作者： Jean-Paul Lallès Division of Human nutrition Division INRA Clermont-Ferrand France Human nutrition research center-West Nantes France

Growing evidence suggests the implication of the gut microbiota in various facets of health and disease. In this review, the focus is put on microbiota-host molecular cross-talk at the gut epithelial level with special emphasis on two defense systems： intestinal alkaline phosphatase（IAP） and inducible heat shock proteins（iHSPs）. Both IAP and iHSPs are induced by various microbial structural components（e.g. lipopolysaccharide, flagellin, CpG DNA motifs）,metabolites（e.g. n-butyrate） or secreted signal molecules（e.g., toxins, various peptides, polyphosphate）. IAP is produced in the small intestine and secreted into the lumen and in the interior milieu. It detoxifies microbial components by dephosphorylation and, therefore, down-regulates microbe-induced inflammation mainly by inhibiting NF-κB pro-inflammatory pathway in enterocytes. IAP gene expression and enzyme activity are influenced by the gut microbiota. Conversely, IAP controls gut microbiota composition both directly, and indirectly though the detoxification of pro-inflammatory free luminal adenosine triphosphate and inflammation inhibition. Inducible HSPs are expressed by gut epithelial cells in proportion to the microbial load along the gastro-intestinal tract. They are also induced by various microbial components, metabolites and secreted molecules. Whether iHSPs contribute to shape the gut microbiota is presently unknown. Both systems display strong anti-inflammatory and anti-oxidant properties that are protective to the gut and the host. Importantly, epithelial gene expressions and protein concentrations of IAP and iHSPs can be stimulated by probiotics, prebiotics and a large variety of dietary components, including macronutrients（protein and amino acids, especially L-glutamine, fat, fiber）, and specific minerals（e.g. calcium）and vitamins（e.g. vitamins K1 and K2）. Some food components（e.g. lectins, soybean proteins, various polyphenols） may inhibit or disturb these systems. The general cel ular and molecular mechanisms involved in the microbiota-host epi

关键词： Diet Gut Inducible heat shock protein Inflammation Intestinal alkaline phosphatase Microbiota

Increasing physical activity among children and adolescents:Innovative ideas needed

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Journal of Sport and Health Science 2019年第1期8卷 1-5页

作者： Tom Baranowski SDA/ARS Children’s nutrition research center Baylor College of Medicine

1.Introduction For anyone paying attention,this is a disruptive time in science in general and in health promotion science in particular.Obesity is the most prevalent nutrition-related health problem around the globe.The issue of whether,as is commonly thought,obesity is causative of early mortality or whether it is only a marker of risk has been raised.The lack of substantial and maintained change in child obesity prevention has been recognized, and numerous limitations in our conceptual and methodological approach to obesity prevention have been identified.

关键词： Increasing physical Introduction paying attention

Potential of essential oils for poultry and pigs

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Animal nutrition 2018年第2期4卷 179-186页

作者： Hengxiao Zhai Hong Liu Shikui Wang Jinlong Wu Anna-Maria Kluenter DSM(China)Animal nutrition research center DSM nutritional Products Ltd.

The increasing pressure of abolishing and/or decreasing the use of antibiotics as antimicrobial growth promoters for livestock calls for alternative solutions to sustain the efficiency of current livestock production.Among the alternatives, essential oils have a great potential and are generally considered natural, less toxic, and free from residues.Essential oils have been proven in numerous in vitro studies to exert antimicrobial effects on various pathogens.The current review touched on the basics of essential oils, and the in vivo effects of essential oils on growth, intestinal microflora, anti-oxidation, immune functionality, meat qualities as well as the possible modes of action in poultry and pigs, and the future research areas were proposed.

关键词： Antibiotics Chickens Essential oils Pathogens Pigs

Lactiplantibacillus plantarum L47 and inulin alleviate enterotoxigenic Escherichia coli induced ileal inflammation in piglets by upregulating the levels of a-linolenic acid and 12,13-epoxyoctadecenoic acid

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Animal nutrition 2023年第3期14卷 370-382页

作者： Leihong Cui Hui Zeng Meixin Hou Zhongxin Li Chunlong Mu Weiyun Zhu Suqin Hang National center for International research on Animal Gut nutrition Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthLaboratory of Gastrointestinal MicrobiologyNanjing Agricultural UniversityNanjing 210095China

Alternatives to antibiotics for preventing bacteria-induced inflammation in early-weaned farm animals are sorely needed. Our previous study showed that Lactiplantibacillus plantarum L47 and inulin could alleviate dextran sulfate sodium(DSS)-induced colitis in mice. To explore the protective effects of L. plantarum L47 and inulin on the ileal inflammatory response in weaned piglets challenged with enterotoxigenic Escherichia coli(ETEC), 28 weaned piglets were assigned into four groups, namely, CON group—orally given 10 mL/d phosphate buffer saline(PBS), LI47 group—orally given a mixture of 10 m L/d L. plantarum L47 and inulin, ECON group—orally given 10 mL/d PBS and challenged by ETEC, and ELI47group—orally given 10 mL/d L. plantarum L47 and inulin mixture and challenged by ETEC. The results demonstrated that the combination of L. plantarum L47 and inulin reduced inflammatory responses and relieved the inflammatory damage caused by ETEC, including ileal morphological damage, reduced protein expression of ileal tight junction, decreased antioxidant capacity, and decreased antiinflammatory factors. Transcriptome analysis revealed that L. plantarum L47 and inulin up-regulated the gene expression of phospholipase A2 group IIA(PLA2G2A)(P < 0.05) as well as affected alphalinolenic acid(ALA) metabolism and linoleic acid metabolism. Moreover, L. plantarum L47 and inulin increased the levels of ALA(P < 0.05), lipoteichoic acid(LTA)(P < 0.05), and 12,13-epoxyoctadecenoic acid(12,13-EpOME)(P < 0.05) and the protein expression of Toll-like receptor 2(TLR2)(P = 0.05) in the ileal mucosa. In conclusion, L. plantarum L47 and inulin together alleviated ETEC-induced ileal inflammation in piglets by up-regulating the levels of ALA and 12,13-EpOME via the LTA/TLR2/PLA2G2A pathway.

关键词： Lactiplantibacillus plantarum L47 Inulin Enterotoxigenic Escherichia coli Ileal inflammation Piglet Phospholipase A2 group IIA

Multi-omics approach in tea polyphenol research regarding tea plant growth,development and tea processing:current technologies and perspectives

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Food Science and Human Wellness 2022年第3期11卷 524-536页

作者： Jingwen Li Yu Wang Joon Hyuk Suh Department of Food Science and Human nutrition Citrus Research and Education CenterUniversity of FloridaFL 33850USA

Polyphenols are one of the most important metabolites in tea due to their unique biological activities and health benefits,arousing great attention of researchers to investigate biochemical mechanisms of polyphenols during tea plant growth,development and tea processing.Although omics has been used as a major analytical platform for tea polyphenol research with some proven merits,a single-omics strategy remains a considerable challenge due to the complexity of biological system and functional processes of tea in each stage of tea production.Recent advances in multi-omics approaches and data analysis have enabled mining and mapping of enormous number of datasets at different biological scales from genotypes to phenotypes of living organisms.These new technologies combining genomics,metagenomics,transcriptomics,proteomics and/or metabolomics can pave a new avenue to address fundamental questions regarding polyphenol formation and changes in tea plants and products.Here,we review recent progresses in single-and multi-omics approaches that have been used in the field of tea polyphenol studies.The perspectives on future research and applications for improvement of tea polyphenols as well as current challenges of multi-omics studies for tea polyphenols are also discussed.

关键词： Multi-omics Single-omics Tea polyphenols Tea breeding and processing

Galacto-oligosaccharides improve barrier function and relieve colonic inflammation via modulating mucosa-associated microbiota composition in lipopolysaccharides-challenged piglets

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Journal of Animal Science and Biotechnology 2022年第1期13卷 240-255页

作者： Ren Gao Shiyi Tian Jing Wang Weiyun Zhu National center for International research on Animal Gut nutrition Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthNational Experimental Teaching Demonstration Center of Animal ScienceLaboratory of Gastrointestinal MicrobiologyCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Background:Galacto-oligosaccharides(GOS)have been shown to modulate the intestinal microbiota of suckling piglets to exert beneficial effects on intestinal function.However,the modulation of intestinal microbiota and intestinal function by GOS in intestinal inflammation injury models has rarely been reported.In this study,we investigated the effects of GOS on the colonic mucosal microbiota composition,barrier function and inflammatory response of lipopolysaccharides(LPS)-challenged suckling piglets.Methods:A total of 18 newborn suckling piglets were divided into three groups,the CON group,the LPS-CON group and the LPS-GOS group.Piglets in the LPS-GOS group were orally fed with 1 g/kg body weight of GOS solution every day.On the d 14,piglets in the LPS-CON and LPS-GOS group were challenged intraperitoneally with LPS solution.All piglets were slaughtered 2 h after intraperitoneal injection and sampled.Results:We found that the colonic mucosa of LPS-challenged piglets was significantly injured and shedding,while the colonic mucosa of the LPS-GOS group piglets maintained its structure.Moreover,GOS significantly reduced the concentration of malondialdehyde(MDA)and the activity of reactive oxygen species(ROS)in the LPS-challenged suckling piglets,and significantly increased the activity of total antioxidant capacity(T-AOC).GOS significantly increased the relative abundance of norank_f_Muribaculaceae and Romboutsia,and significantly decreased the relative abundance of Alloprevotella,Campylobacter and Helicobacter in the colonic mucosa of LPS-challenged suckling piglets.In addition,GOS increased the concentrations of acetate,butyrate and total short chain fatty acids(SCFAs)in the colonic digesta of LPS-challenged suckling piglets.GOS significantly reduced the concentrations of interleukin 1β(IL-1β),interleukin 6(IL-6),tumor necrosis factor-α(TNF-α)and cluster of differentiation 14(CD14),and the relative mRNA expression of Toll-like receptor 4(TLR4)and myeloid differentiation primary response 88(MyD88)in the LPS-challenge

关键词： Barrier function Galacto-oligosaccharides Intestinal microbiota Lipopolysaccharides Suckling piglets

Early-life galacto-oligosaccharides supplementation alleviates the small intestinal oxidative stress and dysfunction of lipopolysaccharide-challenged suckling piglets

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1543-1556页

作者： Shiyi Tian Jue Wang Ren Gao Jing Wang Weiyun Zhu Laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthNational Center for International Research on Animal Gut NutritionNational Experimental Teaching Demonstration Center of Animal ScienceCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China

Background:Galacto-oligosaccharides(GOS)are non-digestible food ingredients that promote the growth of beneficial bacteria in the gut.This study investigated the protective effect of the early-life GOS supplement on the piglets’gut function against the oxidative stress induced by lipopolysaccharide(LPS)-challenge.Methods:Eighteen neonatal piglets were assigned to three groups including CON,LPS and LPS+GOS groups.The piglets in CON group and LPS group received physiological saline,while those in LPS+GOS group received GOS solution for 13 d after birth.On d 14,the piglets in LPS group and LPS+GOS group were injected with LPS solutions,while the piglets in CON group were injected with the same volume of physiological saline.Results:The results showed that the early-life GOS supplement blocked the LPS-induced reactive oxygen species(ROS)secretion,malondialdehyde(MDA)production and the increase of pro-apoptotic factor expression.Meanwhile,the early-life GOS supplement improved the activities of antioxidant enzymes,disaccharidase enzymes activities,and digestive enzymes activities,and increased the mRNA abundance of the gene related to nutrient digestion and absorption and the relative protein expression of tight junction.The study also showed that the early-life GOS supplement improved the expression of Hemeoxygenase-1(HO-1)and NAD(P)H/quinone acceptor oxidoreductase-1(NQO-1),and activated the AMP-activated protein kinase(AMPK).Conclusions:These results suggested that GOS enhanced the gut function,reduced the ROS production and proapoptotic factors gene expression,and activated the AMPK signaling pathway in LPS-challenged piglets.

关键词： Early-life Galacto-oligosaccharides Lipopolysaccharide Oxidative stress Suckling piglets