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DMF-scMT-seq linking methylome and transcriptome within single cells with digital microfluidics

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Science China Chemistry 2024年第6期67卷 2070-2078页

作者： Xing Xu Xi Zeng Xin Lin Shiyan Lin Shanshan Liang Tian Tian Rui Su Jia Song Chaoyong Yang Collaborative Innovation center of Chemistry for Energy materials The MOE Key Laboratory of Spectrochemical Analysis and InstrumentationState Key Laboratory of Physical Chemistry of Solid SurfacesDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringThe First Affiliated Hospital of Xiamen UniversityXiamen UniversityXiamen361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai200127China Chemistry and materials Science College Shanghai Normal UniversityShanghai200234China Chemistry and Biomedicine Innovation center(ChemBIC) School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China

Single-cell joint analysis of methylome and transcriptome reveals how the methylation regulates the transcriptional ***,traditional bench-top protocols for single-cell DNA methylation and RNA transcription co-detection are laborintensive,cost-ineffective and ***,we establish the DMF-sc MT-seq,a highly-efficient and cost-effective method to simultaneously analyze single-cell DNA methylation and transcriptional activity based on digital *** MT-seq automates the workflow of single-cell isolation,cellular hypotonic lysis,nucleic acid separation and methylome/transcriptome library construction in a contactless and addressable *** system ensures high accuracy(R>0.85),high gene detection ability(14,697 genes per cell at 4 million sequencing depth),and high CpG coverage(677,198 CpG sites per cell at 1million sequencing depth).By using DMF-sc MT-seq,the relationship of DNA methylation and RNA transcription under different genomic contexts is *** further apply DMF-sc MT-seq to study the dynamics of transcription regulation with methylation-inhibiting anti-tumor Decitabine,and identify the methylated promoter/gene body driven genes in response to Decitabine ***-sc MT-seq facilitates the construction of the correlation of DNA methylation and transcriptional activity at the single-cell level in a flexible,sensitive and accurate way,which is anticipated to be a powerful tool in studying single-cell biological systems.

关键词： single-cell analysis digital microfluidics methylome sequencing transcriptome sequencing

Experimental observation of multiple topological nodal structure in LaSb_(2)

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 138-145页

作者： Yuxi Qiao Fuyi Wang Huaiqiang Wang Zicheng Tao Zhicheng Jiang Zhengtai Liu Soohyun Cho Fayuan Zhang Qingkai Meng Wei Xia Yichen Yang Zhe Huang Jishan Liu Zhonghao Liu Zengwei Zhu Shan Qiao Yanfeng Guo Haijun Zhang Dawei Shen state Key laboratory of Functional materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China National Synchrotron Radiation laboratory and School of Nuclear Science and TechnologyUniversity of Science and Technology of ChinaHefei 230026China National laboratory of solid state Microstructures School of PhysicsNanjing UniversityNanjing 210093China School of Physics and Technology Nanjing Normal UniversityNanjing 210023China School of Physical Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China Shenzhen Institute for Quantum Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Wuhan National High Magnetic Field center and School of Physics Huazhong University of Science and TechnologyWuhan 430074China ShanghaiTech laboratory for Topological Physics ShanghaiTech UniversityShanghai 201210China Institute of High-Pressure Physics School of Physical Science and Technology Ningbo UniversityNingbo 315211China Collaborative Innovation center of Advanced Microstructures Nanjing UniversityNanjing 210093China center of materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Unconventional fermions in the immensely studied topological semimetals are the source for rich exotic topological ***,using symmetry analysis and first-principles calculations,we propose the coexistence of multiple topological nodal structure in LaSb_(2),including topological nodal surfaces,nodal lines and in particular eightfold degenerate nodal points,which have been scarcely observed in a single ***,utilizing angle-resolved photoemission spectroscopy,we confirm the existence of nodal surfaces and eightfold degenerate nodal points in LaSb_(2).The intriguing multiple topological nodal structure might play a crucial role in giving rise to the large linear *** work renews the insights into the exotic topological phenomena in LaSb_(2).

关键词： eightfold degenerate points multiple topological nodal structure angle-resolved photoemission spectroscopy linear unsaturated magnetoresistance

Raman Scattering from Fine Zn Particles Coated with ZnO

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Chinese Physics Letters 1996年第10期13卷 765-767页

作者： XU Jian-feng CHENG Guang-xu DU You-wei Department of Physics and laboratory of solid state Microstructures center of materials analysis Nanjing UniversityNanjing 210093

Raman scattering from Zn fine spheric particles coated with ZnO of variable thickness is measured, and a broad line can be found. The Raman line shifts to lower frequency side with the increase of oxide thickness. A theoretical analysis based on the existence of surface phonon mode has been made. Agreement between experiment and theory is good.

关键词： Raman scattering theory

Ultrafast photoexcitation dynamics behavior of hydrogen-bonded polyfluorenol

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Chinese Chemical Letters 2024年第1期35卷 486-491页

作者： Man Xu Chuanxin Wei Yunlong Zhang Hao Li Jingyao Ma Jinyi Lin Shengjie Wang Wei Xue Qi Wei Linghai Xie Wei Huang state Key laboratory of Organic Electronics and Information Displays&School of Chemistry and Life Sciences&Institute of Advanced materials(IAM) Nanjing University of Posts&TelecommunicationsNanjing 210023China Key laboratory of Flexible Electronics(KLOFE)&Institute of Advanced materials(IAM) Nanjing Tech University(NanjingTech)Nanjing 211816China Frontiers Science center for Flexible Electronics(FSCFE) Shaanxi Institute of Flexible Electronics(SIFE)&Shaanxi Institute of Biomedical Materials and Engineering(SIBME)Northwestern Polytechnical UniversityXi’an 710072China

Exciton behavior is crucial to the exploitation of light-emitting conjugated polymer(LCPs)for optoelectronic *** excitons are easily trapped by the intrinsically defect ***,we set a polyfluorenol(PPFOH)as an example to systematically investigate its photophysical behavior to check the role of defect structures in *** to time-resolved photoluminescence analysis,the feature emission peaks from individual chain of PPFOH in diluted DMF solution is effectively avoided the influence of fluorenone formation,but the residual green-band emission at 550nm is easily observed in the PL spectra of PPFOH dilute toluene solution obtained delay 1.5 ns,confirmed the formation of“guest”physical aggregation-induced defect ***,efficient and ultrafast energy transfer from individual chain to defect structure is observed in PPFOH ***,the efficient energy transfer happened for the film obtained from DMF solution(200 ps)than those of toluene ones(600 ps).Meanwhile,compared to relatively stable green-band emission in PPFOH film from toluene solution,red-shifted emission peak(11 nm)of PPFOH film from DMF solutions exposed to saturated DNT vapor also confirmed their different aggregation-induced green-band ***,this aggregation defect structures are influenced on the photophysical property of LCPs in solid states.

关键词： Photoexcitation dynamics Polyfluorenol Aggregation behavior Energy transfer Exciton migration

Spin Wave Resonance in Fe/Cu Compositionally Modulated Films

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Chinese Physics Letters 1994年第1期11卷 53-56页

作者： JIN Qingyuan XU Yongbing ZHAI Ya LU Mu BIE Qingshan JIANG Shulin ZHAI Hongru center of materials analysis and National Laboratory of Solid State Microstructure.Nanjing UniversityNanjing 210008

Fe/Cu compositionally modulated films made by rf sputtering were investigated with ferromagnetic *** wave resonances related to interlayer exchange coupling were observed and the phase change of the spin wave was shared by both the Fe and Cu *** effective spin wave dispersion coefficient was found to increase with increasing thickness of Cu sublayers.

关键词： Fe/Cu wave ferromagnetic

Temperature of Transformation from Fullerite to Amorphous Carbon

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Chinese Physics Letters 1995年第4期12卷 207-209页

作者： CHENG Guangxu GU Gang ZANG Wencheng DU Youwei FENG Duan center of materials analysis Nanjing UniversityNanjing 210008 the National laboratory of solid state Microstructures Nanjing UniversityNanjing 210008

Raman spectra during the transformation from fullerite to amorphous carbon are *** transformation temperature is obtained from intensity ratio of Stokes and anti-Stokes lines by using Placzek’s *** temperature,less t... 详细信息

关键词： transformation Transformation Stokes

Phonon Characterization of Raman Spectra in High T_(c) Superconductors Y_(1-x)Gd_(x)Ba_(2)Cu_(3)O_(7-δ)

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Chinese Physics Letters 1994年第1期11卷 31-34页

作者： ZHANG Mingsheng CHEN Qiang JIN Xin YIN Zhen YAO Xixian center for materials analysis National Laboratory of Solid State Microstructures Department of Physics Nanjing UniversityNanjing 210008

Concentration-dependent Raman spectra of Y_(1-x)Gd_(x)Ba_(2)Cu_(3)O_(7-δ)(x=0.4,0.6,0.8 and 1.0)show that the Raman mode from O(4)bridging shifts upward due to the increased symmetry of crystal *** linewidth and intensity of 508cm^(-1)mode are decreased and increased with increasing x,respectively which is attributed to the charge transfer from the CuO basal planes to the CuO_(2)*** second-order Raman modes at 798 and 896cm^(-1)have been observed and confirmed.

关键词： mode Superconductor

Blue-Light Emission from Nanocrystalline La_(0.5)Na_(0.5)TiO^(3)

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Chinese Physics Letters 1998年第10期15卷 758-760页

作者： ZHANG Wei-feng ZHANG Xing-tang YIN Zhen ZHANG Ming-sheng MA Guo-hong DU Zu-liang center for materials analysis and National laboratory of solid state Microstructures Nanjing UniversityNanjing 210093 Department of Physics He’nan UniversityKaifeng 475001

Nanocrystalline La_(0.5)Na_(0.5)TiO^(3) powders were prepared by the stearic acid-gel method,and the grain sizes could be controlled by varying annealing *** sizes were determined to be from 14 to 50nm using Scherrer's formula.A very strong blue-light emission band around 480nm was clearly observed in the nano-sized La_(0.5)Na_(0.5)TiO^(3) with a grain size of 14nm at room ***,nano-size effects on the luminescence properties were *** results could be explained with the aid of a self-trapped exciton model.

关键词： temperature. sizes sized

Dielectric Characteristics and Layer Periodic Effects of Ferroelectric Superlattice BaTiO_(3)/SrTiO_(3)

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Chinese Physics Letters 2001年第9期18卷 1271-1274页

作者： ZHANG Jiang YIN Zhen ZHANG Ming-Sheng CHEN Wan-Chun National laboratory of solid state Microstructures and center for materials analysis Nanjing UniversityNanjing 210093 Institute of Physics Chinese Academy of SciencesBeijing 100080

A pseudospin-phonon coupled model in double-time Green's function theory has been developed to investi- gate the dielectric susceptibility of the ferroelectric-dielectric superlattice BaTiO_(3)/SrTiO_(3) (BTO/STO). As the pseudospin-pseudospin and pseudospin-phonon interactions increase, the dielectric susceptibility changes from a narrow shape into the strong and broad shape with the peak shifting to a higher temperature region. With increasing pseudospin-phonon interaction in the BTO layer and at smallpseudospin-pseudospin interaction in BTO layer, the dielectric susceptibility was sensitively adjusted around the transition temperature of strontium titanate, and at large pseudospin-pseudospin interaction in the BTO layer, the dielectric susceptibility was increased in the high-temperature region covering the tetragonal-cubic transition temperature of barium titanate. A critical period of 10/I0 in the BTO/STO superlattice has been obtained, which is in good agreement with the experimental data of 40A, for ten unit cells.

关键词： dielectric susceptibility interaction