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ASFV pp62 Protein Has 9 Dominant B-T Cell Combined Epitopes

Animal Husbandry and Feed science 2022年第4期14卷 13-20页

作者： Cao Qianda Dai Bihong Yang Yang Cao Xuelian Fushun Animal Disease Control center Zigong 643200China Neijiang Vocational&Technical College Neijiang 641000China Yanjiang Animal Disease Control center Ziyang 641300China

[Objective]The paper was to study the basic characteristics of the pp62 protein of the African swine fever virus strain Pig/HLJ/2018,and to provide more basic data for the study of the virus.[Method]We used the ProtParam program to analyze the physical and chemical properties of the pp62 ***-2.0 and SignalP-5.0 were used to analyze protein transmembrane region and signal peptide,and Lasergene 7.0 Protean program was used to study protein antigen index,hydrophilicity,surface accessibility and titration *** N-glycosylation site and O-glycosylation site came out with NetNGlyc-1.0 and NetOGlyc-4.0 online ***,PSIPRED-4.0,NetSurfP-2.0,and PSRSM were used to analyze protein secondary structure,BepiPred1.0 and IEDB tools were used to analyze protein B-cell epitopes,and NetMHC 4.0 and NetMHCpan tools were used to analyze protein T-cell *** we used Swiss-Model to analyze the high-level structure of the protein,the EzMol tool to visually analyze B-T cell combined epitopes,and finally,mega 7.0 to analyze the genetic evolutionary relationship of the protein.[Result]The pp62 protein of African swine fever viral strain Pig/HLJ/2018 had a molecular weight of 60.5 *** was a hydrophilic acid labile protein,and had no transmembrane region and signal *** were 5 N-glycosylation sites and 4 O-glycosylation *** of the secondary structure of the protein showed that the proportions of helix,coil and strands were 45.5%,41.7%and 12.8%,*** study of dominant epitopes revealed that there were 14 dominant B-cell epitopes and 16 dominant T-cell *** 9 dominant B-T cell combined epitopes located on the surface of the protein molecule were *** phylogenetic tree constructed with the pp62 protein showed that the evolutionary relationship of Pig/HLJ/2018 strain was the closest to Georgia/2007/1,which belonged to genotype II.[Conclusion]The results will provide basic information for pp62 research.

关键词： African swine fever virus pp62 CP530R Dominant B-T cell combined epitope Phylogenetic tree Visual analysis

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Identification of Lablab Semen Album by DNA Barcode Technology

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Medicinal Plant 2017年第6期8卷 45-47页

作者： Huiming LUO Jian RAO Bingyi XIAO Ping NIE Hai LIN Ye DING Hunan Institute for Drug Control Pharmaceutical Quality Evaluation Engineering Research center of Hunan Province

[Objectives] To identify ITS2 barcode of Lablab Semen Album and its adulterants,and provide a new method for the identification of Lablab Semen Album. [Methods] The ITS2 sequence was amplified by PCR and sequenced bi-directionally. After splicing by Codon Code Aligner,the data were processed with the aid mega software to construct the cluster dendrogram( neighbor-joining,NJ tree). [Results]The ITS2 sequence of Lablab Semen Album had length of 218 bp; the constructed cluster dendrogram indicated that all species were monophyletic and could be distinguished from other species. [Conclusions] The ITS2 barcode can be used for rapid identification of Lablab Semen Album and its adulterants and this experiment further verified that DNA barcode technology is effective in identification of traditional Chinese medicines.

关键词： DNA barcode ITS2 Lablab Semen Album PCR amplification Identification

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One stop shop Ⅱ:taxonomic update with molecular phylogeny for important phytopathogenic genera:26–50(2019)

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Fungal Diversity 2019年第1期 41-129页

作者： Ruvishika S.Jayawardena Kevin D.Hyde Rajesh Jeewon Masoomeh Ghobad-Nejhad Dhanushka N.Wanasinghe NingGuo Liu Alan J.L.Phillips Jose Ribamar COliveira-Filho Gladstone Ada Silva Tatiana B.Gibertoni P.Abeywikrama L.M.Carris K.W.T.Chethana A.J.Dissanayake S.Hongsanan S.C.Jayasiri A.R.McTaggart R.H.Perera K.Phutthacharoen K.G.Savchenko R.G.Shivas Naritsada Thongklang Wei Dong DePing Wei Nalin NWijayawardena Ji-Chuan Kang The Engineering Research center of Southwest Biopharmaceutical Resources Ministry of EducationGuizhou UniversityGuiyang 550025People’s Republic of China center of Excellence in Fungal Research Mae Fah Luang UniversityChiang RaiThailand Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China Department of Health sciences Faculty of ScienceUniversity of MauritiusReduitMauritius Department of Biotechnology Iranian Research Organization for Science and Technology(IROST)P.O.Box 15815-3538Tehran 15819Iran World agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Universidade de Lisboa Faculdade de CienciasBiosystems and Integrative Sciences Institute(BioISI)Campo Grande1749-016 LisbonPortugal Departamento de Micologia Universidade Federal de PernambucoAvenida da EngenhariaS/N-Cidade UniversitariaRecifePE 50740-600Brazil Beijing Key Laboratory of Environmet Friendly Management on Fruit Disease and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097People’s Republic of China Department of Plant Pathology Washington State UniversityPullmanWA 99164USA College of Life science and Oceanography ShenZhen University1068Nanhai AvenueNanshanShenzhen 518055China Queensland Alliance for Agriculture and Food Innovation The University of QueenslandBrisbaneQLD 4001Australia Department of Biological sciences Butler UniversityIndianapolisIN 46208USA Centre for Crop Health University of Southern QueenslandToowoombaQLD 4350Australia Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Faculty of Agriculture National Resources and EnvironmentNaresuan UniversityPhitsanulok 65000Thailand

This paper is the second in a series focused on providing a stable platform for the taxonomy of phytopathogenic *** focuses on 25 phytopathogenic genera:Alternaria,Bipolaris,Boeremia,Botryosphaeria,Calonectria,Coniella,Corticiaceae,Curvularia,Elsinoe,Entyloma,Erythricium,Fomitiporia,Fulviformes,Laetisaria,Limonomyces,Neofabraea,Neofusicoccum,Phaeoacremonium,Phellinotus,Phyllosticta,Plenodomus,Pseudopyricularia,Tilletia,Venturia and Waitea,using recent molecular data,up to date names and the latest taxonomic *** each genus a taxonomic background,diversity aspects,species identification and classification based on molecular phylogeny and recommended genetic markers are *** this study,varieties of the genus Boeremia have been elevated to species ***,Bipolaris,Curvularia,Neofusicoccum and Phyllosticta that were included in the One Stop Shop 1 paper are provided with updated entries,as many new species have been introduced to these genera.

关键词： Boeremia DNA barcodes Endophytes Plant pathology Systematics Taxonomy

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Cryobiological Characteristics of L-proline in Mammalian Oocyte Cryopreservation

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Chinese Medical Journal 2016年第16期129卷 1963-1968页

作者： Lu Zhang Xu Xue Jie Yan Li-Ying Yan Xiao-Hu Jin Xiao-Hui Zhu Zhi-Zhu He Jing Liu Rong Li Jie Qiao Department of Obstetrics and Gynecology Center for Reproductive Medicine Peking University Third Hospital Beijing 100191 China Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing 100191 China Beijing Key Laboratory of Cryo-Biomedical Engineering and Key Laboratory of Cryogenics Technical Institute of Physics and Chemistry Chinese Academy of Sciences Beijing 100190 China Key Laboratory of Assisted Reproduction Ministry of Education Beijing 100191 China Department of Biomedical Engineering School of Medicine Tsinghua University Beijing 100084 China

Background： L-proline is a natural, nontoxic cryoprotectant that helps cells and tissues to tolerate freezing in a variety of plants and animals. The use of L-proline in mammalian oocyte cryopreservation is rare. In this study, we explored the cryobiological characteristics of L-proline and evaluated its protective effect in mouse oocyte cryopreservation. Methods： The freezing property of L-proline was detected by Raman spectroscopy and osmometer. Mature oocytes obtained from 8-week-old B6D2F 1 mice were vitrified in a solution consisting various concentration of L-proline with a reduced proportion ofdimethyl sulfoxide （DMSO） and ethylene glycol （EG）, comparing with the control group （15% DMSO and 15% EG without L-proline）. The survival rate, 5-methylcytosine （5-mC） expression, fertilization rate, two-cell rate, and blastocyst rate in vitro were assessed by immunofluorescence and in vitro fertilization. data were analyzed by Chi-square test. Results： L-proline can penetrate the oocyte membrane within 1 min. The osmotic pressure of 2.00 mol/L L-proline mixture is similar to that of the control group. The survival rate of the postthawed oocyte in 2.00 mol/L L-proline combining 7.5% DMSO and 10% EG is significantly higher than that of the control group. There is no difference of 5-mC expression between the L-proline combination groups and control. The fertilization rate, two-cell rate, and blastocyst rate in vitro from oocyte vitrified in 2.00 mol/L L-proline combining 7.5% DMSO and 10% EG solution are similar to that of control. Conclusions： It indicated that an appropriate concentration of L-proline can improve the cryopreservation efficiency of mouse oocytes with low concentrations of DMSO and EG, which may be applicable to human oocyte vitrification.

关键词： Cryoprotective Agentant Mouse Oocyte Vitrification

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