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Crystal Structure Theory and Applications 2019年第1期8卷 1-11页

作者： Myriam Louati Regaya Ksiksi Laurent Jouffret Mohamed Faouzi Zid University of Tunis El Manar Faculty of Sciences of Tunis Laboratory of Materials Crystal Chemistry and Applied Thermodynamics Tunis Tunisia The Higher Institute of Preparatory Studies in biology and Geology (ISEP-BG) of Soukra Carthage University Tunisia Tunisia Clermont Auvergne University Clermont-Ferrand France

A new decavanadate, (C4N2H7)4(C6N2H10)V10O28·2H2O, was synthesized by slow evaporation at room temperature and characterized by single crystal X-ray diffraction, IR and UV-Vis spectroscopies. The compound crystallizes in the triclinic system, P-1 space group with the cell parameters: a = 11.200(5) Å, b = 12.056(9) Å, c =20.511(7) Å, α = 73.40(4), β = 84.67(3), γ = 63.51(5), Z = 2 and volume V = 2373.7(1) Å3. The formula unit is composed of one decavanadate [V10O28]6- anion, four 2-methylimidazolium (C4N2H7)+ cations, one 2-amino-4-picolinium (C6N2H10)2+ cation and two water molecules. In the crystal, the layers of decavanadate groups, organic cations and water molecules stack up parallel to the (011) plane. The cohesion is provided by N-H···O, O-H···O hydrogen bonds and Van der Waals interactions leads to a three-dimensional structure. The absorbance spectrum measurement shows an optical band gap of 3.27 eV which allows us to conclude that this compound is a semiconductor material.

关键词： Decavanadate Synthesis X-Ray Analysis Crystal Structure Semiconductor Material

Transporting Mammalian cells at Ambient Temperature:A Viable Alternative to Dry Ice

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Advances in Bioscience and Biotechnology 2017年第4期8卷 127-133页

作者： Steingrimur Stefansson Seunghyun Han Ye In Jeon David S.Chung Peter Hwang Huyen Le John L.Warden David Ho HeMemics Biotechnologies Inc RockvilleUSA Johns Hopkins University BaltimoreUSA George Washington University WashingtonDCUSA Division of biology and Medicine Brown UniversityProvidenceUSA Thomas Jefferson High School for Science and Technology AlexandriaUSA Nauah Solutions McleanUSA

The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and then packaging them in dry ice for shipment. However many countries place restrictions on dry ice shipments. An alternative to shipping frozen cell vials is to send flasks of growing cells in media. This also has problems because cells in media have limited viability and the flasks can leak. Here we report on an alternative method for shipping viable cells at ambient temperature without dry ice or in media filled flasks. In this study we report on the development and properties of HemSol?. This is an inexpensive, eco-friendly and protects cell integrity at ambient temperature while maintaining viability. We have previously shown that HemSol? protects platelet and RBC function in cold storage and circulating tumor cells up to 6 days. Therefore we wanted to know if HemSol? could also be used to transport live cells. Since HemSol? is a liquid, we experimented with encasing the cells with HemSol? and gelatin so as to prevent dry ice shipment of cells and circumvent the shipping of cells in media. We performed mock shipping experiments where cells were stored in HemSol? gel kept at room temperature on a lab benchtop and cells stored in dry ice was also kept on lab benchtop for up to 2 days. After the mock shipping period, we analyzed cells for their functions. Our results show that cells in HemSol? gel have greater than 95% viability and restored biological functions in 2 hours, whereas, cells shipped in dry ice required more than 24 hours to recover and needed media change to remove the DMSO.

关键词： HemSol^(TM) HemSol^(TM) Gel Transportation Short Term Storage cells Shipment Dry Ice Ambient Temperature Shipment

血红素氧合酶-2基因缺失对氧化应激性脑损伤的保护作用(英文)

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中华神经外科疾病研究杂志 2005年第3期4卷 197-203页

作者：屈延章翔陈晶马新 Raymond F.Regan 第四军医大学西京医院神经外科 Department of Emergency Medicine Thomas Jefferson University 1020 Sansom Street Thompson Building Room 239 Philadelphia PA 19107 USA

目的探讨血红素氧合酶2基因缺失对血红素诱导氧化应激性脑损伤的保护作用。方法分别将6μl(8μmol/L)灭菌氯高铁血红素定向注入野生型小鼠和基因(HO2)敲除小鼠的纹状体内,72h后分别检测纹状体细胞生存率,蛋白和脂类的氧化作用。用蛋白... 详细信息

目的探讨血红素氧合酶2基因缺失对血红素诱导氧化应激性脑损伤的保护作用。方法分别将6μl(8μmol/L)灭菌氯高铁血红素定向注入野生型小鼠和基因(HO2)敲除小鼠的纹状体内,72h后分别检测纹状体细胞生存率,蛋白和脂类的氧化作用。用蛋白质印迹法检测血红素氧合酶1,2(HO1)的表达。结果与野生型相比,基因(HO2)敲除小鼠纹状体内蛋白和脂类的氧化作用显著降低,而纹状体细胞的存活率显著增加;HO1的表达在两种小鼠注射前后没有明显差异。结论结果提示,血红素氧合酶2基因缺失对血红素诱导的氧化应激性脑损伤具有保护作用;选择性抑制神经元血红素氧合酶2基因的表达可减轻氧化应激性脑损伤。

关键词：血红素氧合酶基因缺失氧化应激脑损伤

CD4 T cell deficiency attenuates ischemic stroke, inhibits oxidative stress, and enhances Akt/mTOR survival signaling pathways in mice

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Chinese Neurosurgical Journal 2018年第4期4卷 189-195页

作者： Hongfei Zhang Xiaoxing Xiong Lijuan Gu Weiying Xie Heng Zhao Department of Neurosurgery Stanford University School of Medicine1201 Welch Rd.MSLS Bldg.Room P306StanfordCA 94305USA Department of Anesthesiology Zhujiang Hospital of Southern Medical UniversityGuangzhouGuangdongChina Department of Neurosurgery Renmin Hospital of Wuhan UniversityWuhanHubeiChina Central Laboratory Renmin Hospital of Wuhan UniversityWuhanHubeiChina Department of Anesthesiology Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouChina

Background: Inhibition of CD4 T cells reduces stroke-induced infarction by inhibiting neuroinflammation in the ischemic brain in experimental stroke. Nevertheless, little is known about its effects on neuronal survival signaling pathways. In this study, we investigated the effects of CD4 T cell deficits on oxidative stress and on the Akt/mTOR cell signaling pathways after ischemic stroke in mice. Methods: MHC II gene knockout C57/BL6 mice, with significantly decreased CD4 T cells, were used. Stroke was induced by 60-min middle cerebral artery (MCA) occlusion. Ischemic brain tissues were harvested for Western blotting. Results: The impairment of CD4 T cell production resulted in smaller infarction. The Western blot results showed that iNOS protein levels robustly increased at 5 h and 24 h and then returned toward baseline at 48 h in wild-type mice after stroke, and gene KO inhibited iNOS at 5 h and 24 h. In contrast, the anti-inflammatory marker, arginase I, was found increased after stroke in WT mice, which was further enhanced in the KO mice. In addition, stroke resulted in increased phosphorylated PTEN, Akt, PRAS40, P70S6, and S6 protein levels in WT mice, which were further enhanced in the animals whose CD4 T cells were impaired. Conclusion: The impairment of CD4 T cell products prevents ischemic brain injury, inhibits inflammatory signals, and enhances the Akt/mTOR cell survival signaling pathways.

关键词： Stroke Neuroinflammation CD4 T cells Akt MTOR PTEN

Quantitative evaluation of DNA damage caused by atmospheric and room-temperature plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding

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Chinese Journal of Chemical Engineering 2021年第11期34卷 205-210页

作者： Yuting Huang Liyang Wang Xue Zhang Nan Su Heping Li Yoshimitsu Oda Xinhui Xing Key Laboratory for Industrial Biocatalysis Ministry of Education of ChinaDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Biobreeding Research Center Wuxi Research Institute of Applied TechnologiesTsinghua UniversityWuxi 214072China Department of Entomology College of Plant ProtectionChina Agricultural UniversityBeijing 100193China Department of Engineering Physics Tsinghua UniversityBeijing 100084China Institute of Life and Environmental Sciences Osaka Shin-Ai CollegeTsurumiTsurumi-kuOsaka 5380052Japan Centre for Synthetic and Systems biology Tsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 518055China

Mutagenesis is an important technique for microbial mutation *** the source of mutations,DNA damage extent is a key indicator for the effectiveness of ***,a rapid and easy DNA damage quantification method is required for the comparison of mutagenesis effects and development of mutagenesis ***,we used the umu-microplate test system to quantitatively compare the DNA damage strength caused by atmospheric and room-temperature plasma(ARTP)and other traditional mutagenesis methods including:ultraviolet radiation(UV),diethyl sulfate(DES)and 4-nitroquinoline-1-oxide(4-NQO).The test strain of Salmonella typhimurium TA1535/pSK1002 was used to monitor the time-course profile of b-galactosidase activity induced by DNA damage caused by different mutagenesis methods using a microplate *** umu-microplate test results showed that ARTP caused higher extent of DNA damage than UV and chemical mutagens,which agrees well with the result obtained by SOS-FACS-based quantification method as reported *** umu-microplate test is accessible for broad researchers who are lack of the expensive FACS instruments and allows the quick quantitative evaluation of DNA damage among living cells for different mutagenesis methods in the study of the microbial mutation breeding.

关键词： ARTP mutagenesis umu-Microplate test Biological engineering cell engineering Biotechnology

Inhibitory effects of antisense phosphorothioate oligodeoxynucleotides on pancreatic cancer cell Bxpc-3 telomerase activity and cell growth in vitro

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World Journal of Gastroenterology 2006年第25期12卷 4004-4008页

作者： Yun-Feng Wang Ke-Jian Guo Bei-Ting Huang Yong Liu Xiao-Yun Tang Jian-Jun Zhang Qiang Xia Department of General Surgery The Center Hospital of Shanghai Yangpu District (Branch Hospital of The Affiliated Xinhua Hospital of Shanghai Jiaotong University) Shanghai 200127 China Center of Liver Transplantation of The Affiliated Renji Hospital of Shanghai Jiaotong University Shanghai 200127 China Department of Biliary-Pancreatic Surgery The First Affiliated Hospital of China Medical University Shengyang 110001 Liaoning Province China Department of Clinical Epidemiology Staff Room of the First Affiliated Hospital of China Medical University Shengyang 11001 Liaoning Province China The First Department of The Center of Experiment and Technology The China Medical University Shengyang 11001 Liaoning Province China

AIM： To investigate the effect of telomerase hTERT gene antisense oligonucleotide （hTERT-ASO） on proliferation and telomerase activity of pancreatic cancer cell line Bxpc-3. METHODS： MTT assay was used to detect the effect of different doses of hTERT-ASO on proliferation of Bxpc-3 cell for different times. To study the anti-tumor activity, the cells were divided into there groups： Control group （pancreatic cancer cell Bxpc-3）; antisense oligonucleotide （hTERT-ASO） group; and nosense oligonucleotide group decorated with phosphorothioate. Telomerase activity was detected using TRAP-PCR-ELISA. cell DNA distribution was examined using flow cytometry assay. cell apoptosis was observed by transmission electron microscope in each group. RESULTS： After treatment with 6 mmollL hTERT- ASO, cell proliferation was inhibited in dose- and time- dependent manner. The telomerase activity decreased after treatment with hTERT-ASO for 72 h. Flow cytometry showed the cell number of G0/G1 phase increased from 2.7% to 14.7%, the cell number of S phase decreased from 72.7% to 51.0%, and a sub-G1 stage cell apoptosis peak appeared in front of G1 stage. CONCLUSION： Telomerase antisense oligodeoxy- nucleotide can inhibit the proliferation of pancreatic cancer cell line Bxpc-3 and decrease the telomerase activity and increase cell apoptosis rate in vitro.

关键词： Antisense oligodeoxynucleotide hTERT Telomerase Telomerase reverse transcriptase

建立MRI监测胆道内局部试剂运送技术:应用猪的可行性研究

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国际医学放射学杂志 2012年第3期 270-270页

作者： S.S.Soriano P.Willis 刘学焕 Image-guided Bio-Molecular Intervention Research and Section of Vascular and Interventional Radiology Department of RadiologyInstitute for Stem Cell and Regenerative MedicineUniversity of Washington School of Medicine815 Mercer StRoom S470SeattleWA 98109

目的研究MR成像监测胆管内运送钆莫特沙芬(MGd)进入猪胆总管(CBD)壁的可行性。材料与方法动物实验获机构动物保护和使用委员会批准。首先,采用不同浓度的MGd,T1加权MR成像对比剂、化疗药物及细胞标记物的混合物,对人胆管癌细胞进行处理... 详细信息

目的研究MR成像监测胆管内运送钆莫特沙芬(MGd)进入猪胆总管(CBD)壁的可行性。材料与方法动物实验获机构动物保护和使用委员会批准。首先,采用不同浓度的MGd,T1加权MR成像对比剂、化疗药物及细胞标记物的混合物,对人胆管癌细胞进行处理。然后,使用共焦显微镜检

关键词：锥虫蓝 CBD 离体试验

Progress in the Pathogenesis and Treatment of Radiation-Induced Brain Injury

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Proceedings of Anticancer Research 2020年第6期4卷 20-26页

作者： Xiaohan Gao Xiaohan Wu Xiaoqian Yang Shaoshui Chen Hongmin Zhi Department of Oncology Binzhou Medical University HospitalBinzhou 256603Shandong ProvinceChina Department of Operating room of Anesthesiology Binzhou Medical University HospitalBinzhou 256603Shandong ProvinceChina

Malignant tumors are one of the serious public health problems that threaten the survival time of human *** are prone to metastasis to distant organs and the central nervous system is one of the common target *** it is difficult for chemotherapeutics,targeted drugs and other macromolecules to pass through the blood brain barrier(BBB),local radiation therapy is often used for treating intracranial primary or metastatic ***,whether it is whole brain radiation therapy(WBRT)or stereotactic body radiation therapy(SBRT),the choice of radiation dose is limited by the side effects of radiation therapy on the surrounding normal brain ***-induced brain injury(RBI)can further develop into radiation necrosis(RN)in the late *** is often effective against RBI by antagonizing vascular endothelial growth factor(VEGF),but it still cannot completely reverse *** treatment options such as human pluripotent stem-cell transplantation have made it possible to reverse the process of RN.

关键词： radiation brain injury radiation necrosis VEGF stem cell transplantation

Neutrophil depletion-but not prevention of Kupffer cell activation-decreases the severity of cerulein-induced acute pancreatitis

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World Journal of Gastroenterology 2006年第8期12卷 1219-1224页

作者： Catherine M Pastor Alain Vonlaufen Fabianna Georgi Antoine Hadengue Philippe Morel Jean-Louis Frossard Laboratoire de Physiopathologie Hépatique et Imagerie Moléculaire H■pitaux Universitaires de GenèveB■timent CRoom 6-79524 Rue Micheli-du-Crest1205 GenevaSwitzerland Division of Gastroentérologie et Hépatologie H■pitaux Universitaires de Genève24 Rue Micheli-du-Crest1205 GenevaSwitzerland Département APSI H■pitaux Universitaires de Genève24 Rue Micheli-du-Crest1205 GenevaSwitzerland Département de Chirurgie H■pitaux Universitaires de Genève24 Rue Micheli-du-Crest1205 GenevaSwitzerland

AIM： To determine whether neutrophil depletion and Kupffer cell inhibition might combine their protective effects to decrease the severity of acute pancreatitis. METHODS： Nice had cerulein administration to induce acute pancreatitis and were pretreated with either anti-mouse neutrophil serum or gadolinium chloride （GdCh） to prevent Kupffer cell activation, or both treatments. Injury was assessed in pancreas and lungs. Myeloperoxidases （MPO） assessed neutrophil infiltration. Interleukin-6 （IL-6） and IL-10 were measured in serum, pancreas, lungs and liver. RESULTS： In mice with acute pancreatitis, neutrophil depletion reduced the severity of pancreatitis and pancreatitis-associated lung injury. Kupffer cell inactivation by GdCh had less protective effect, although IL-6 and IL-10 concentrations were significantly decreased. The protective treatment brought by neutrophil depletion was not enhanced by Kupffer cell inactivation and both treatments did not combine their protective effects. CONCLUSION： Our results confirm the role of activated neutrophils in aggravating organ injury in acute pancreatitis while the role of Kupffer cell activation is less obvious.

关键词： Acute pancreatitis Cytokines Neutrophils Kupffer cells Pulmonary injury

Transcriptomics and metabolomics analyses provide insights into postharvest ripening and senescence of tomato fruit under low temperature

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Horticultural Plant Journal 2023年第1期9卷 109-121页

作者： Chunmei Bai Caie Wu Lili Ma Anzhen Fu Yanyan Zheng Jiawei Han Changbao Li Shuzhi Yuan Shufang Zheng Lipu Gao Xinhua Zhang Qing Wang Demei Meng Jinhua Zuo State Key Laboratory of Food Nutrition and Safety College of Food Science and EngineeringTianjin University of Science&TechnologyTianjin 300457China College of Light Industry and Food Engineering Nanjing Forestry UniversityNanjingJiangsu 210037China Key laboratory of vegetable postharvest processing Ministry of AgricultureBeijing Key Laboratory of Fruit and Vegetable Storage and ProcessingKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)of Ministry of AgricultureKey Laboratory of Urban Agriculture(North)of Ministry of Agriculture and Rural AffairsInstitute of Agri-food Processing and NutritionBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China National Engineering Research Center for Information Technology in Agriculture Beijing 100097China School of Agricultural Engineering and Food Science Shandong University of TechnologyZiboShandong 255049China

Tomato is one of the most important vegetable crops in the world and is a model plant used to study the ripening of climacteric fleshy *** the ripening process of tomato fruit,flavor and aroma metabolites,color,texture and plant hormones undergo significant ***,low temperatures delayed the ripening process of tomato fruit,inhibiting flavor compounds and ethylene *** and transcriptomics analyses of tomato fruit stored under low temperature(LT,5°C)and room temperature(RT,25°C)were carried out to investigate the effects of storage temperature on the physiological changes in tomato fruit after *** results of transcriptomics changes revealed that the differentially expressed genes(DEGs)involved in tomato fruit ripening,including several kinds of transcription factors(TFs)(TCP,WRKY,MYB and bZIP),enzymes involved in cell wall metabolism[beta-galactosidase(β-GAL),pectinesterase(PE)and pectate lyase(PL),cellulose and cellulose synthase(CESA)],enzymes associated with fruit flavor and aroma[acetyltransferase(AT),malic enzyme(ME),lipoxygenase(LOX),aldehyde dehydrogenase(ALDH),alcohol dehydrogenase(ADH)and hexokinase(HK)],genes associated with heat stress protein 70 and genes involved in the production of plant hormones such as Ethylene responsive factor 1(ERF1),Auxin/indoleacetic acids protein(AUX/IAA),gibberellin regulated *** on the above results,we constructed a regulatory network model of the effects of different temperatures during the fruit ripening *** to the analysis of the metabolomics results,it was found that the contents of many metabolites in tomato fruit were greatly affected by storage temperature,including,organic acids(L-tartaric acid,a-hydroxyisobutyric acid and 4-acetamidobutyric acid),sugars(melezitose,beta-Dlactose,D-sedoheptulose 7-phosphate,2-deoxyribose 1-phosphate and raffinose)and phenols(coniferin,curcumin and feruloylputrescine).This study revealed the effects of storage tempera

关键词： Metabolomics Tomato Transcriptomics Temperature Fruit ripening