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手足口病急诊留观患儿转归的相关因素分析

护理管理杂志 2014年第4期14卷 238-239,292页

作者：张丽月 Denise Kudirka Colleen Church 肖玉娟吴闯孙玉贝林刚曦厦门大学附属第一医院福建医科大学教学医院儿科厦门市361003 Emergency room Montreal Children's Hospital Emergency room British-Columbia Children's Hospital 同安区新民镇卫生院内儿科厦门市361100

目的探讨儿科手足口病患儿的临床特点及急诊留观患儿转归的危险因素,为手足口病患儿的护理提供依据。方法对2012年8月至2013年3月儿科急诊留观室收治的471例手足口病患儿及同期住院的40例患儿一般资料、临床症状及实验室检查结果进行比... 详细信息

目的探讨儿科手足口病患儿的临床特点及急诊留观患儿转归的危险因素,为手足口病患儿的护理提供依据。方法对2012年8月至2013年3月儿科急诊留观室收治的471例手足口病患儿及同期住院的40例患儿一般资料、临床症状及实验室检查结果进行比较,分析急诊留观患儿转归的危险因素。结果 3岁以下、高热、肢体抖动、血糖大于7.0 mmol/L、白细胞增高的手足口病患儿作为独立的危险因素使患儿转入住院的机会增多(P<0.01或P<0.05)。结论关注高危急诊留观患儿,有助于及时发现手足口病患儿的病情变化,提高其治愈率。存在病情加重危险的患儿,必要时住院进一步治疗。

关键词：手足口病儿科急诊留观室护理管理

Determination of a astragaloside Ⅳ derivative LS-102 in plasma by ultra-performance liquid chromatography-tandem mass spectrometry in dog plasma and its application in a pharmacokinetic study

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Determination of a astragaloside Ⅳ derivative LS-102 in pla...

Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy

首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会

作者： Wen-Xia Sun Zhi-Feng Zhang Li-Sheng Ding Lin-Sen Qing Pei Luo Chengdu Institute of biology Chinese Academy of Sciences Sichuan Industrial Institute of Antibiotics Chengdu University State Key Laboratories for Quality Research in Chinese Medicines Macau University of Science and Technology

Background Astragalosidic acid(LS-102) is a new water-soluble derivative of astragaloside Ⅳ-a major effective component isolated from the Chinese herb Astragali *** previous study showed that LS-102 exhibited potent cardiovascular *** The objective of this study was to investigate the pharmacokinetic properties of LS-102 after single-dose,oral administration in beagle dogs by developing and validating an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) *** and result The chromatographic separation was performed on a Acquity HSS C18 column(100 mm×2.1 mm, 1.8 μm) by a gradient elution using a mobile phase consisting of water and acetonitrile at a flow rate of 0.35 mL/*** analytes were detected with a triple quadrupole tandem mass spectrometry(MS) in multiple reaction monitoring(MRM) mode and a positive ion electrospray ionization(ESI) of the transitions at m/z821.5→627.5 for *** validation revealed a wide linearity over the range of 2.0-10000 ng/mL together with satisfactory intra-and inter-day precision,accuracy,and *** testing showed that LS-102 spiked into dog plasma was stable for 4 h at room temperature,for up to 2 weeks at-80℃, and during three freeze-thaw *** method was effectively and successfully applied to the pharmacokinetics of LS-102 after oral administration(5,10 and 20 mg/kg) to beagle *** plasma concentrations are attained within approximately 2 h after oral administration with a half-life ranging from 1.55 h to 4.49 *** plasma concentration-time curve of LS-102 after oral administration presents the phenomenon of a double-peak absorption *** peak concentration and area under the concentration-time curve of LS-102 seemed to increase with the increasing doses proportionally,that suggesting linear pharmacokinetics in ***,the doxorubicin(Dox)-injured H9 c2 cell model was prepared by incubating the cells in 1 μM Dox for 24 *** assay and L

关键词： astragalosidic acid astragaloside Ⅳ derivative pharmacokinetics bioavailability UPLC-MS/MS

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Journal of Genetics and Genomics 2012年第5期39卷 209-215页

作者： Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao State Key Laboratory of Brain and Cognitive Science Institute of Biophysics The Chinese Academy of Sciences Datun Road 15 Beijing 100101 China Key Laboratory of cell Proliferation and Differentiation of Ministry of Education College of Life Sciences Peking University Beijing 100871 China Department of Biological Science Florida State University Tallahassee Florida 32304-4295 USA

Technology development has always been one of the forces driving breakthroughs in biomedical research. Since the time of Thomas Morgan, Drosophilists have, step by step, developed powerful genetic tools for manipulating and functionally dissecting the Drosophila genome, but room for improving these technologies and developing new techniques is still large, especially today as biologists start to study systematically the functional genomics of different model organisms, including humans, in a high-throughput manner. Here, we report, for the first time in Drosophila, a rapid, easy, and highly specific method for modifying the Drosophila genome at a very high efficiency by means of an improved transcription activator-like effector nuclease （TALEN） strategy. We took advantage of the very recently developed ＂unit assembly＂ strategy to assemble two pairs of specific TALENs designed to modify the yellow gene （on the sex chromosome） and a novel autosomal gene. The mRNAs of TALENs were subsequently injected into Drosophila embryos. From 31.2% of the injected Fo fertile flies, we detected inheritable modification involving the yellow gene. The entire process from construction of specific TALENs to detection of inheritable modifications can be accomplished within one month. The potential applications of this TALEN-mediated genome modification method in Drosophila are discussed.

关键词： TALEN Drosophila Genomic modification Unit assembly Reverse genetics

AGE-induced neuronal cell death is enhanced in G2019S LRRK2 mutation with increased RAGE expression

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Translational Neurodegeneration 2018年第1期7卷 1-8页

作者： Hyun Jin Cho Chengsong Xie Huaibin Cai Department of Biochemistry and Biomedical Sciences Seoul National UniversityCollege of Medicine28 Yungun-dongJongro-guSeoul 110-799South Korea Transgenics Section Laboratory of NeurogeneticsNational Institute on AgingNational Institutes of HealthBuilding 35Room 1A112MSC 370735 Convent DriveBethesdaMD 20892–3707USA

Background:Leucine-rich repeat kinase 2(LRRK2)mutations represent the most common genetic cause of sporadic and familial Parkinson’s disease(PD).Especially,LRRK2 G2019S missense mutation has been identified as the most prevalent genetic cause in the late-onset *** glycation end products(AGEs)are produced in high amounts in diabetes and diverse aging-related disorders,such as cardiovascular disease,renal disease,and neurological *** trigger intracellular signaling pathway associated with oxidative stress and inflammation as well as cell ***,receptor of AGEs,is activated by interaction with AGEs and mediates AGE-induced *** AGE and RAGE are involved in the pathogenesis of mutant LRRK2 is ***:Using cell lines transfected with mutant LRRK2 as well as primary neuronal cultures derived from LRRK2 wild-type(WT)and G2019S transgenic mice,we compared the impact of AGE treatment on the survival of control and mutant cells by *** also examined the levels of RAGE proteins in the brains of transgenic mice and PD patients by western ***:We show that LRRK2 G2019S mutant-expressing neurons were more sensitive to AGE-induced cell death compared to ***,we found that the levels of RAGE proteins were upregulated in LRRK2 G2019S mutant ***:These data suggest that enhanced AGE-RAGE interaction contributes to LRRK2 G2019S mutation-mediated progressive neuronal loss in PD.

关键词： Parkinson’s disease LRRK2 G2019S AGE RAGE Neuronal death

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病毒唑抑制呼吸道合胞病毒的实验研究

中国误诊学杂志 2001年第4期1卷 516-517页

作者：廖传胜柴万新余道文 YU Dao-wen 广东肇庆卫生学校儿科教研室 526020 武汉协和医院儿科 430022 Pediatric Teaching and Studing room of Zhaoqing Hygiene School

目的　研究病毒唑 (三氮唑核苷 )在细胞培养中抑制呼吸道合胞病毒 (RSV)的作用。方法　用细菌培养法 ,采用 Vero细胞感染病毒后 2 h给药法 ,观察细胞病变 (CPE)。结果　当病毒感染量小于或等于 10 0 TCID5 0 时 ,病毒唑组无 CPE,而病毒... 详细信息

目的　研究病毒唑 (三氮唑核苷 )在细胞培养中抑制呼吸道合胞病毒 (RSV)的作用。方法　用细菌培养法 ,采用 Vero细胞感染病毒后 2 h给药法 ,观察细胞病变 (CPE)。结果　当病毒感染量小于或等于 10 0 TCID5 0 时 ,病毒唑组无 CPE,而病毒对照组有 2 5 %～ 75 %的 CPE。病毒唑抑制 RSV的最大无毒浓度 (TD0 ) ,半数有效浓度 (IC5 0 ) ,最小有效浓度 (MTC)和治疗指数 (TI)分别为 10 0、5 0、2 5 μg/ ml和 4。结论　病毒唑在细菌培养中确有显著抑制 RSV的作用 ,这为其治疗 RSV感染提供了实验依据。

关键词：细支气管炎药物疗法抗病毒药治疗

Gastric adenosquamous carcinoma with an elevated serum level of alpha-fetoprotein:A case report

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World Journal of Gastrointestinal Surgery 2023年第10期15卷 2357-2361页

作者： Liang Sun Juan-Juan Wei Ran An Hui-Yun Cai Yuan Lv Tao Li Xiao-Fei Shen Jun-Feng Du Gang Chen Department of General Surgery The 7th Medical Center of PLA General HospitalBeijing 100700China Operating room Department of AnesthesiologyThe 7th Medical Center of PLA General HospitalBeijing 100700China Department of Pathology The 7th Medical Center of PLA General HospitalBeijing 100700China

BACKGROUND Gastric adenosquamous carcinoma(ASC)is rare and characterized by coexisting of adenocarcinoma andsquamous carcinoma within the same *** present a female patient with gastric ASC who had an elevated serum level of alpha-fetopro-tein(AFP),which decreased to normal levels after a laparoscopic distant radical gastrectomy in a short *** clinicopathological features in AFP-producing gastric cancer(GC)are discussed,as well as potentially available prognostic *** SUMMARY A 50-year-old woman presented to our department with a chief complain of a 6-mo history of *** had no basic diseases including heart diseases and respiratory diseases,and she also denied any prior history of dysphagia,hematemesis,melena,rectal bleeding,hematochezia,or unintentional weight *** on her symptoms,an esophagogastroduodenoscopy was performed,showing an annular cavity lesion 3 cm from the pylorus with a diameter of 6 cm.A biopsy of the lesion showed gastric ASC,whereas the pylorus biopsy showed normal *** patient further received an enhanced computed tomography scan which demonstrated an invasive lesion close to the pylorus with a still clear margin of the tumor to peripheral organs such as the pancreas and *** lymph nodes were visible around,whereas no sign of liver metastasis was *** tumor markers including carcinoembryonic antigen(CEA),cancer antigen 199(CA199),CA724,CA125,and CA242 were all normal,while the level of serum AFP increased to 172 ng/mL.A laparoscopic distant radical gastrectomy was performed after exclusion of surgical *** pathology results showed that the tumor displayed an ulcerated ASC phenotype(90%of medium to highly-differentiated squamous cell carcinoma,10%of poorly differentiated ***,the serum level of AFP decreased to normal level on post operation day *** tumor cells were positive for CK5/6,p63,and CEA,and negative for AFP and Epstein-Ba

关键词： Gastric cancer Gastric adenosquamous carcinoma Alpha-fetoprotein Case report

Comparison of human amniotic fluid-derived and umbilical cord Wharton＇s Jelly-derived mesenchymal stromal cells： Characterization and myocardial differentiation capacity

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Journal of Geriatric Cardiology 2012年第2期9卷 166-171页

作者： Jing Bai Yuan Hu Yi-Ru Wang Li-Feng Liu Jie Chen Shao-Ping Su Yu Wang Department of Cardiology The First Affiliated Hospital of Chinese PLA General Hospital 51 Fueheng Road Haidian District Belling 100048 China Institute of Orthopedics The First Affiliated Hospital of Chinese PLA General Hospital 51 Fucheng Road Haidian District Beijing 100048 China Institute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road Haidian District Beijing 100853 China The 9^th Consulting room Chinese PLA General Hospital 28 Fuxing Road Haidian District Beijing 100853 China

Objective To compare the characterization and myocardial differentiation capacity of arnniotic fluid-derived mesenchymal stromal cells （AF MSCs） and umbilical cord Wharton＇s Jelly-derived mesenchymal stromal cells （WJ MSCs）. Methods The human AF MSCs were cultured from amniotic fluid samples obtained by amniocentesis. The umbilical cord WJ MSCs were obtained from Wharton＇s Jelly of umbilical cords of infants delivered full-term by normal labor. The morphology, growth curves, and analyses by flow cytometry of cell surface markers were compared between the two types of cells. Myocardial genes （GATA-4, c-TnT, a-actin, and Cx43） were detected by real-time PCR and the corresponding protein expressions were detected by Western blot analysis after myocardial induced in AF MSCs and WJ MSCs. Results Our findings revealed AF MSCs and WJ MSCs shared similar morphological characteristics of the fibroblastoid shape. The AF MSCs were easily obtained than the WJ MSCs and had a shorter time to reach adherence of 2.7 ± 1.6 days to WJ MSCs of 6.5 ± 1.8 days. The growth curves by MTT cytotoxic assay showed the AF MSCs had a similar proliferative capacity at passage 5 and passage 10. However, the proliferative capacities ofWJ MSCs were decreased at 5 passage relative to 10 passage. Both AF stem cells and WJ stem cells had the characteristics of mesenchymal stromal cells with some characteristics of embryonic stem cells. They express CD29 and CD105, but not CD34. They were positive for Class I major histocompatibility （MHC I） antigens （HLA-ABC）, and were negative, or mildly positive, for MHC Class II （HLA-DR） antigen. Oct-4 was positive in all the two cells types. Both AF MSCs and WJ MSCs could differentiate along myocardium. The differentiation capacities were detected by the expression of GATA-4, c-TnT, a-actin, Cx43 after myocardial induction. Conclusions Both AF MSCs and WJ MSCs have the potential clinical application for myogenesis in cardiac regenerative therapy.

关键词： Human amniotic fluid-derived ceUs Wharton's Jelly-derived cells Myocardial differentiation Mesenchymal stromal cells

Aminoflavone upregulates putative tumor suppressor miR-125b-2-3p to inhibit luminal A breast cancer stem cell-like properties

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Precision Clinical Medicine 2022年第2期5卷 72-86页

作者： Nicole Mavingire Petreena Campbell Tiantian Liu Jonathan Wooten Salma Khan Xin Chen Jason Matthews Charles Wang Eileen Brantley Department of Basic Sciences Loma Linda University School of MedicineLoma LindaCA 92350USA Center for Genomics Loma Linda University School of MedicineLoma LindaCA 92350USA Center for Health Disparities and Molecular Medicine Loma Linda University School of MedicineLoma LindaCA 92350USA Department of Nutrition University of OsloOslo 0372Norway Department of Pharmacology and Toxicology University of TorontoTorontoOntario M5S 1A8Canada Frederick National Laboratory for Cancer Research PO Box BBldg.432Room 232 FrederickMD 21702-1201USA

Metastatic breast cancer is incurable and often due to breast cancer stem cell(CSC)-mediated *** previously determined that the aryl hydrocarbon receptor(AhR)agonist aminoflavone(AF)inhibits the expression of the CSC biomarkerα6-integrin(ITGA6)to disrupt the formation of luminal(hormone receptor-positive)mammospheres(3D breast cancer spheroids).In this study,we performed miRNA-sequencing analysis of luminal A MCF-7 mammospheres treated with AF to gain further insight into the mechanism of AF-mediated anti-cancer and anti-breast CSC *** significantly induced the expression of>70 microRNAs(miRNAs)including miR125b-2–3p,a predicted stemness gene ***-mediated miR125b-2–3p induction was validated in MCF-7 mammospheres and ***125b-2–3p levels were low in breast cancer tissues irrespective of subtype compared to normal breast *** miR125b-2–3p levels were low in MCF-7 cells,they were much lower in AHR100 cells(MCF-7 cells made unresponsive to AhR agonists).The miR125b-2–3p mimic decreased,while the antagomiR125b-2–3p increased the expression of stemness genes ITGA6 and SOX2 in MCF-7 *** MCF-7 mammospheres,the miR125b-2–3p mimic decreased only ITGA6 expression although the antagomiR125b-2–3p increased ITGA6,SOX2 and MYC ***125b-2–3p reversed AF-mediated suppression of *** miR125b-2–3p mimic decreased proliferation,migration,and mammosphere formation while the antagomiR125b-2–3p increased proliferation and mammosphere formation in MCF-7 *** miR125b-2–3p mimic also inhibited proliferation,mammosphere formation,and migration in AHR100 *** induced AhR-and miR125b2-3p-dependent anti-proliferation,anti-migration,and mammosphere disruption in MCF-7 *** findings suggest that miR125b-2–3p is a tumor suppressor and AF upregulates miR125b-2–3p to disrupt mammospheres via mechanisms that rely at least partially on AhR in luminal A breast cancer cells.

关键词： breast cancer cancer stem cells therapeutic targeting miR125b-2-3p aminoflavone aryl hydrocarbon receptor mammospheres

MicroRNA-375 Suppresses the Tumor Aggressive Phenotypes of Clear cell Renal cell Carcinomas through Regulating YWHAZ

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Chinese Medical Journal 2018年第16期131卷 1944-1950页

作者： Xiang Zhang Nai-Dong Xing Cheng-Jun Lai Rui Liu Wei Jiao Jue Wang Jie Song Zhong-Hua Xu Department of Urology Qilu Hospital of Shandong University Jinan Shandong 250012 China Teaching and Research Institute of Urology School of Medicine Shandong University Jinan Shandong 250012 China Department of Urology Qingyun People's Hospital Dezhou Shandong 253700 China Department of the First Operation room Qilu Hospital of Shandong University Jinan Shandong 250012 China Central Laboratory The Second Hospital of Shandong University Jinan Shandong 250012 China Medicaid and Health Care Service Office Qilu Hospital of Shandong University Jinan Shandong 250012 China

Background： MicroRNAs （miRNAs） are key regulators during tumor initiation and progression. MicroRNA-375 （MiR-375） has been proven to play a tumor-suppressive role in various types of human malignancies; however, its biological role in clear cell renal cell carcinoma （ccRCC） remains unclear. The purpose of this study was to explore the biologic role as well as the underlying mechanism of miR-375 in ccRCC progression. Methods： Quantitative polymerase chain reaction （qPCR） was applied to test the expression of miR-375 in tissues and cell lines by t-test. Functional experiments were used to investigate the biological role of miR-375 utilizing a gain-of-function strategy. The target of miR-375 was investigated by bioinformatic analysis and further verified by luciferase reporter assay, qPCR, Western blotting, and functional experiments in vitro. Results： Our study demonstrated that miR-375 was significantly downregulated in ccRCC tissues （cancer vs. normal, 0.804 ±0.079 vs. 1.784 ± 0.200, t = 5.531 P 〈 0.0001 ） and cell lines, and loss ofmiR-375 expression significantly associated with advanced Fuhrman nuclear grades （Grade Ⅲ and Ⅳ vs. Grade Ⅰ and Ⅱ, 1.000 ± 0.099 vs. 1.731 ± 0.189, t = 3.262 P = 0.003）. Functional studies demonstrated that miR-375 suppressed ccRCC cell proliferation, migration, and invasion （all P 〈 0.05 in both 786-0 and A498 cell lines）. Multiple miRNA target prediction algorithms indicated the well-studied oncogene YWHAZ as a direct target ofmiR-375, which was further confirmed by the luciferase reporter assay, qPCR, and Western blotting. Moreover, restoration of YWHAZ could rescue the antiproliferation effect ofmi R-375. Conclusions： The data provide the solid evidence that miR-375 plays a tumor-suppressive role in ccRCC progression, partially through regulating YWHAZ. This study expands the antitumor profile ofmiR-375, and supports its role as a potential therapeutic target in ccRCC treatment.

关键词： Clear cell Renal cell Carcinoma MicroRNA-375 Proliferation YWHAZ