检索结果-南通市图书馆

Current status and future prospects of stomatology research

在线全文

学校读者我要写书评

暂无评论

Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2023年第10期24卷 853-867,I0001页

作者： Qianming CHEN Yahui WANG Jing SHUAI Stomatology Hospital School of StomatologyZhejiang University School of MedicineClinical Research Center for Oral Diseases of Zhejiang ProvinceKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhou 310006China

Research in stomatology(dental medicine)continues to expand globally and is oriented towards solving clinical issues,focusing on clarifying the clinical relevance and potential mechanisms of oral–systemic connections via clinical epidemiology,oral microecological characterization,and the establishment of animal models.Interdisciplinary integration of materials science and tissue engineering with stomatology is expected to lead to the creation of innovative materials and technologies to better resolve the most prevalent and challenging clinical issues such as peri-implantitis,soft and hard tissue defects,and dentin hypersensitivity.With the rapid development of artificial intelligence(AI),5th generation mobile communication technology(5G),and big data applications,“intelligent stomatology”is emerging to build models for better clinical diagnosis and management,accelerate the reform of education,and support the growth and advancement of scientific research.Here,we summarized the current research status,and listed the future prospects and limitations of these three aspects,aiming to provide a basis for more accurate etiological exploration,novel treatment methods,and abundant big data analysis in stomatology to promote the translation of research achievements into practical applications for both clinicians and the public.

关键词： Stomatology Dental medicine Systemic disease Material Innovative technique

Circulating tumor DNA analysis:potential to revise adjuvant therapy for stage II colorectal cancer

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第10期7卷 3613-3615页

作者： Ning Zhu Hanguang Hu Ying Yuan Department of Medical Oncology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiangChina cancer Institute Key Laboratory of Cancer Prevention and InterventionMinistry of EducationThe Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiangChina cancer center Zhejiang UniversityHangzhouZhejiangChina

In the DYNAMIC multicenter phase 2 study recently published in The New England Journal of Medicine,Tie J.et al.demonstrated that a novel circulating tumor DNA(ctDNA)-guided regimen reduced the use of adjuvant chemotherapy without sacrificing recurrence-free survival in patients with stage II colorectal cancer(CRC).1 This was the first reported prospective,randomized,controlled trial of ctDNA-based interventional adjuvant treatment for stage II CRC.

关键词： adjuvant colorectal al.

KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Oral Science 2022年第2期14卷 220-230页

作者： Zhuo Chen Wenzhi Wu Chen Zheng Yanhua Lan Huizhi Xie Zhijian Xie Stomatology Hospital School of StomatologyZhejiang University School of MedicineClinical Research Center for Oral Diseases of Zhejiang ProvinceKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhouChina

Multiple signaling pathways are involved in the regulation of cell proliferation and differentiation in odontogenesis and dental tissue renewal,but the details of these mechanisms remain unknown.Here,we investigated the expression patterns of a transcription factor,Krüppel-like factor 6(KLF6),during the development of murine tooth germ and its function in odontoblastic differentiation.KLF6 was almost ubiquitously expressed in odontoblasts at various stages,and it was co-expressed with P21(to varying degrees)in mouse dental germ.To determine the function of Klf6,overexpression and knockdown experiments were performed in a mouse dental papilla cell line(iMDP-3).Klf6 functioned as a promoter of odontoblastic differentiation and inhibited the proliferation and cell cycle progression of i MDP-3 through p21 upregulation.Dual-luciferase reporter assay and chromatin immunoprecipitation showed that Klf6 directly activates p21 transcription.Additionally,the in vivo study showed that KLF6 and P21were also co-expressed in odontoblasts around the reparative dentin.In conclusion,Klf6 regulates the transcriptional activity of p21,thus promoting the cell proliferation to odontoblastic differentiation transition in vitro.This study provides a theoretical basis for odontoblast differentiation and the formation of reparative dentine regeneration.

关键词： KLF6 inhibited details

Remodeling of the osteoimmune microenvironment after biomaterials implantation in murine tibia: Single-cell transcriptome analysis

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第4期22卷 404-422页

作者： Jia Li Congrui Zhao Yangbo Xu Lu Song Yanqi Chen Yuzi Xu Yang Ma Siyuan Wang Antian Xu Fuming He Department of Prosthodontics Stomatology HospitalSchool of StomatologyZhejiang University School of MedicineZhejiang Provincial Clinical Research Center for Oral DiseasesKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhouChina

Osseointegration seems to be a foreign body reaction equilibrium due to the complicated interactions between the immune and skeletal systems.The heterogeneity of the osteoimmune microenvironment in the osseointegration of implant materials remains elusive.Here,a single-cell study involving 40043 cells is conducted,and a total of 10 distinct cell clusters are identified from five different groups.A preliminary description of the osteoimmune microenvironment revealed the diverse cellular heterogeneity and dynamic changes modulated by implant properties.The increased immature neutrophils,Ly6C+CCR2hi monocytes,and S100a8hi macrophages induce an aggressive inflammatory response and eventually lead to the formation of fibrous capsule around the stainless steel implant.The enrichment of mature neutrophils,FcgR1hi and differentiated immunomodulatory macrophages around the titanium implant indicates favorable osseointegration under moderate immune response.Neutrophil-depletion mice are conducted to explore the role of neutrophils in osseointegration.Neutrophils may improve bone formation by enhancing the recruitment of BMSCs via the CXCL12/CXCR3 signal axis.These findings contribute to a better knowledge of osteoimmunology and are valuable for the design and modification of‘osteoimmune-smart’biomaterials in the bone regeneration field.

关键词： Osteoimmunology Neutrophil Implant Osseointegration Single-cell transcriptomics

Application of silk fibroin coatings for biomaterial surface modification:a silk road for biomedicine

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2023年第11期24卷 943-956页

作者： Jinxing HU Zhiwei JIANG Jing ZHANG Guoli YANG Stomatology Hospital School of StomatologyZhejiang University School of MedicineZhejiang Provincial Clinical Research Center for Oral DiseaseKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityEngineering Research Center of Oral Biomaterials and Devices of Zhejiang ProvinceHangzhou310000China

Silk fibroin(SF)as a natural biopolymer has become a popular material for biomedical applications due to its minimal immunogenicity,tunable biodegradability,and high biocompatibility.Nowadays,various techniques have been developed for the applications of SF in bioengineering.Most of the literature reviews focus on the SF-based biomaterials and their different forms of applications such as films,hydrogels,and scaffolds.SF is also valuable as a coating on other substrate materials for biomedicine;however,there are few reviews related to SF-coated biomaterials.Thus,in this review,we focused on the surface modification of biomaterials using SF coatings,demonstrated their various preparation methods on substrate materials,and introduced the latest procedures.The diverse applications of SF coatings for biomedicine are discussed,including bone,ligament,skin,mucosa,and nerve regeneration,and dental implant surface modification.SF coating is conducive to inducing cell adhesion and migration,promoting hydroxyapatite(HA)deposition and matrix mineralization,and inhibiting the Notch signaling pathway,making it a promising strategy for bone regeneration.In addition,SF-coated composite scaffolds can be considered prospective candidates for ligament regeneration after injury.SF coating has been proven to enhance the mechanical properties of the substrate material,and render integral stability to the dressing material during the regeneration of skin and mucosa.Moreover,SF coating is a potential strategy to accelerate nerve regeneration due to its dielectric properties,mechanical flexibility,and angiogenesis promotion effect.In addition,SF coating is an effective and popular means for dental implant surface modification to promote osteogenesis around implants made of different materials.Thus,this review can be of great benefit for further improvements in SF-coated biomaterials,and will undoubtedly contribute to clinical transformation in the future.

关键词： Silk fibroin Coating Surface modification Notch signaling pathway

The dichotomous role of immunoproteasome in cancer:Friend or foe?

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第5期13卷 1976-1989页

作者： Boya Chen Haiying Zhu Bo Yang Ji Cao Institute of Pharmacology and Toxicology Zhejiang Province Key Laboratory of Anti-Cancer Drug ResearchCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China The Innovation Institute for Artificial Intelligence in Medicine Zhejiang UniversityHangzhou 310058China Cancer Center of Zhejiang University Hangzhou 310058China

Immunoproteasome is a variant of proteasome with structural differences in 20S subunits optimizing them for the production of antigenic peptides with higher binding affinity to major histocompatibility complex(MHC)-I molecules.Apart from this primary function in antigen presentation,immunoproteasome is also responsible for the degradation of proteins,both unfolded proteins for the maintenance of protein homeostasis and tumor suppressor proteins contributing to tumor progression.The altered expression of immunoproteasome is frequently observed in cancers;however,its expression levels and effects vary among different cancer types exhibiting antagonistic roles in tumor development.This review focuses on the dichotomous role of immunoproteasome in different cancer types,as well as summarizes the current progression in immunoproteasome activators and inhibitors.Specifically targeting immunoproteasome may be a beneficial therapeutic intervention in cancer treatment and understanding the role of immunoproteasome in cancers will provide a significant therapeutic insight for the prevention and treatment of cancers.

关键词： Immunoproteasome Ubiquitin—proteasome system Antigenic peptides Proteolysis cancer Immunotherapy Proteasome inhibitor Targeted therapy

AKT inhibitor Hu7691 induces differentiation of neuroblastoma cells

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第4期13卷 1522-1536页

作者： Shaowei Bing Senfeng Xiang Zhimei Xia Yilong Wang Zhonghai Guan Jinxin Che Aixiao Xu Xiaowu Dong Ji Cao Bo Yang Jinhu Wang Qiaojun He Meidan Ying Department of Surgical Oncology Children's HospitalZhejiang University School of MedicineHangzhou 310052China Institute of Pharmacology and Toxicology Zhejiang Province Key Laboratory of Anti-Cancer Drug ResearchCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China cancer center Zhejiang UniversityHangzhou 310058China Innovation Institute for Artificial Intelligence in Medicine Zhejiang UniversityHangzhou 310058China Pediatric cancer Research center National Clinical Research Center for Child HealthHangzhou 310052China

While neuroblastoma accounts for 15%of childhood tumor-related deaths,treatments against neuroblastoma remain scarce and mainly consist of cytotoxic chemotherapeutic drugs.Currently,maintenance therapy of differentiation induction is the standard of care for neuroblastoma patients in clinical,especially high-risk patients.However,differentiation therapy is not used as a first-line treatment for neuroblastoma due to low efficacy,unclear mechanism,and few drug options.Through compound library screening,we accidently found the potential differentiation-inducing effect of AKT inhibitor Hu7691.The protein kinase B(AKT)pathway is an important signaling pathway for regulating tumorigenesis and neural differentiation,yet the relation between the AKT pathway and neuroblastoma differentiation remains unclear.Here,we reveal the anti-proliferation and neurogenesis effect of Hu7691 on multiple neuroblastoma cell lines.Further evidence including neurites outgrowth,cell cycle arrest,and differentiation mRNA marker clarified the differentiation-inducing effect of Hu7691.Meanwhile,with the introduction of other AKT inhibitors,it is now clear that multiple AKT inhibitors can induce neuroblastoma differentiation.Furthermore,silencing AKT was found to have the effect of inducing neuroblastoma differentiation.Finally,confirmation of the therapeutic effects of Hu7691 is dependent on inducing differentiation in vivo,suggesting that Hu7691 is a potential molecule against neuroblastoma.Through this study,we not only define the key role of AKT in the progression of neuroblastoma differentiation but also provide potential drugs and key targets for the application of differentiation therapies for neuroblastoma clinically.

关键词： Neuroblastoma Differentiation therapy High-risk AKT AKT inhibitor Target therapy

The impact of lipids on the cancer—immunity cycle and strategies for modulating lipid metabolism to improve cancer immunotherapy

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2023年第4期13卷 1488-1497页

作者： Mingming Zheng Wenxin Zhang Xi Chen Hongjie Guo Honghai Wu Yanjun Xu Qiaojun He Ling Ding Bo Yang zhejiang Province Key Laboratory of Anti-cancer Drug Research Institute of Pharmacology and ToxicologyCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Medical Thoracic Oncology the Cancer Hospital of University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Chinese Academy of SciencesHangzhou 310022China The Innovation Institute for Artificial Intelligence in Medicine Zhejiang UniversityHangzhou 310018China Cancer Center of Zhejiang University Hangzhou 310058China

Lipids have been found to modulate tumor biology,including proliferation,survival,and metastasis.With the new understanding of tumor immune escape that has developed in recent years,the influence of lipids on the cancer—immunity cycle has also been gradually discovered.First,regarding antigen presentation,cholesterol prevents tumor antigens from being identified by antigen presenting cells.Fatty acids reduce the expression of major histocompatibility complex class I and costimulatory factors in dendritic cells,impairing antigen presentation to T cells.Prostaglandin E2(PGE2)reduce the accumulation of tumor-infiltrating dendritic cells.Regarding T-cell priming and activation,cholesterol destroys the structure of the T-cell receptor and reduces immunodetection.In contrast,cholesterol also promotes T-cell receptor clustering and relative signal transduction.PGE2 represses T-cell proliferation.Finally,regarding T-cell killing of cancer cells,PGE2 and cholesterol weaken granule-dependent cytotoxicity.Moreover,fatty acids,cholesterol,and PGE2 can improve the activity of immunosuppressive cells,increase the expression of immune checkpoints and promote the secretion of immunosuppressive cytokines.Given the regulatory role of lipids in the cancer—immunity cycle,drugs that modulate fatty acids,cholesterol and PGE2 have been envisioned as effective way in restoring antitumor immunity and synergizing with immunotherapy.These strategies have been studied in both preclinical and clinical studies.

关键词： Lipids Fatty acids Cholesterol Prostaglandin E2 Tumor immune escape cancer—immunity cycle Immunotherapy Combination therapy

Deubiquitination complex platform:A plausible mechanism for regulating the substrate specificity of deubiquitinating enzymes

在线全文

学校读者我要写书评

暂无评论

药学学报:英文版 2023年第7期13卷 2955-2962页

作者： Yi-Zheng Fang Li Jiang Qiaojun He Ji Cao Bo Yang Institute of Pharmacology and Toxicology Zhejiang Province Key Laboratory of Anti-Cancer Drug ResearchCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China The Innovation Institute for Artificial Intelligence in Medicine Zhejiang UniversityHangzhou 310018China Cancer Center of Zhejiang University Hangzhou 310058China center for Drug Safety Evaluation and Research of zhejiang university Hangzhou 310058China

Deubiquitinating enzymes(DUBs) or deubiquitinases facilitate the escape of multiple proteins from ubiquitin-proteasome degradation and are critical for regulating protein expression levels in vivo.Therefore,dissecting the underlying mechanism of DUB recognition is needed to advance the development of drugs related to DUB signaling pathways.To data,extensive studies on the ubiquitin chain specificity of DUBs have been reported,but substrate protein recognition is still not clearly understood.As a breakthrough,the scaffolding role may be significant to substrate protein selectivity.From this perspective,we systematically characterized the scaffolding proteins and complexes contributing to DUB substrate selectivity.Furthermore,we proposed a deubiquitination complex platform(DCP) as a potentially generic mechanism for DUB substrate recognition based on known examples,which might fill the gaps in the understanding of DUB substrate specificity.

关键词： Deubiquitinating enzyme(DUB) Deubiquitination Substrate specificity Complex Scaffold Protein partner