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Time windows for postnatal changes in morphology and membrane excitability of genioglossal and oculomotor motoneurons

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World Journal of Neurology 2015年第4期5卷 113-131页

作者： Livia Carrascal JoséLuis Nieto-González Ricardo Pardillo-Díaz Rosario Pásaro Germán Barrionuevo Blas Torres William E Cameron Pedro Nú?ez-Abades department of Physiology University of Seville department of Neurocience University of Pittsburgh department of Behavioral Neuroscience Oregon Health and Science University departamento de Fisiología Facultad de Farmacia Universidad de Sevilla

Time windows for postnatal changes in morphology and membrane excitability of genioglossal(GG) and oculomotor(OCM) motoneurons(MNs) are yet to be fully described. Analysis of data on brain slices in vitro of the 2 populations of MNs point to a well-defined developmental program that progresses with common age-related changes characterized by:(1) increase of dendritic surface along with length and reshaping of dendritic tree complexity;(2) disappearance of gap junctions early in development;(3) decrease of membrane passive properties, such as input resistance and time constant, together with an increase in the number of cells displaying sag, and modifications in rheobase;(4) action potential shortening and afterhyperpolarization; and(5) an increase in gain and maximum firing frequency. These modifications take place at different time windows for each motoneuronal population. In GG MNs, active membrane properties change mainly during the first postnatal week, passive membrane properties in the second week, and dendritic increasing length and size in the third week of development. In OCM MNs, changes in passive membrane properties and growth of dendritic size take place during the first postnatal week, while active membrane properties and rheobase change during the second and third weeks of development. The sequential order of changes is inverted between active and passive membrane properties, and growth in size does not temporally coincide for both motoneuron populations. These findings are discussed on the basis of environmental cues related to maturation of the respiratory and OCM systems.

关键词： development Motoneurons Respiratory system Oculomotor system Neuronal plasticity

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Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit

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Horticulture Research 2019年第1期6卷 1638-1654页

作者： JoséG.Vallarino delphine M.Pott Eduardo Cruz-Rus Luis Miranda Juan J.Medina-Minguez Victoriano Valpuesta Alisdair R.Fernie JoséF.Sánchez-sevilla Sonia Osorio Iraida Amaya department of Molecular Biology and Biochemistry Instituto de Hortofruticultura Subtropical y Mediterránea“La Mayora”University of Málaga–Consejo Superior de Investigaciones Científicas(IHSM-UMA-CSIC)Campus de Teatinos29071 MálagaSpain Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany Genómica y Biotecnología Centro de MálagaInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)29140 MálagaSpain Ingeniería y Tecnología Agroalimentaria Centro Las Torres-TomejilInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)Alcaládel RíoSevillaSpain Ingeniería y Tecnología Agroalimentaria Centro de HuelvaInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)HuelvaSpain

Improvement of nutritional and organoleptic quality of fruits is a key goal in current strawberry breeding *** ratio of sugars to acids is a determinant factor contributing to fruit liking,although different sugars and acids contribute in varying degrees to this complex trait.A segregating F1 population of 95 individuals,previously characterized for several fruit quality characters,was used to map during 2 years quantitative trait loci(QTL)for 50 primary metabolites,L-ascorbic acid(L-AA)and other related traits such as soluble solid content(SSC),titratable acidity(TA),and pH.A total of 133 mQTL were detected above the established thresholds for 44 *** 12.9%of QTL were detected in the 2 years,suggesting a large environmental influence on primary metabolite *** objective of this study was the identification of key metabolites that were associated to the overall variation in SSC and *** it was observed in previous studies,a number of QTL controlling several metabolites and traits were co-located in homoeology group V(HG V).mQTL controlling a large variance in raffinose,sucrose,succinic acid,and L-AA were detected in approximate the same chromosomal regions of different homoeologous linkage groups belonging to HG *** genes for selected mQTL are proposed based on their co-localization,on the predicted function,and their differential gene expression among contrasting F1 progeny ***-seq analysis from progeny lines contrasting in L-AA content detected 826 differentially expressed genes and identified Mannose-6-phosphate isomerase,FaM6PI1,as a candidate gene contributing to natural variation in ascorbic acid in strawberry fruit.

关键词： straw breeding trait

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肝硬化患者谷氨酰胺酶活性增加,其增加与轻微肝性脑病相关

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世界核心医学期刊文摘（胃肠病学分册） 2005年第1期1卷 59-60页

作者： Romero-Gómez M. Ramos-Guerrero R. Grande L. 黎一兵 Hepatology Unit Hospital Universitario de ValmeCtra Cádiz S/n41014SevillaSpain

Background/Aims We performed the current study to assess the intestinal activity of enterocyte phosphate-activated glutaminase(PAG) in cirrhosis. Methods Forty-nine cirrhotic patients and 36 control subjects underwent endoscopic duodenal biopsies. Minimal hepatic encephalopathy (MHE) was evaluated using three psychometric tests. Oral glutamine challenge(OGC) was performed and MELD, Child-Pugh and the presence of esophageal varices were recorded. PAG was measured by enzymatic methods. Cerebral magnetic resonance spectroscopy was performed in 10 cirrhotics. Results PAG was found to be higher in cirrhotics than control subjects 2.4± 1.51vs.0.68± 0.57 IU/mg protein (P dependently related to altered enterocyte PAG. Conclusions Enterocyte PAG is increased in cirrhotic patients and correlates with MHE. These data support a possible role for intestinal glutaminase in the pathogenesis of hepatic encephalopathy (HE) and could be a new target for future therapies.

关键词：肝性脑病酶活性激发试验血清胆汁酸活性磷酸盐肠上皮细胞肝脏病组织活检心理测试酶法

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HVEM,a cosignaling molecular switch,and its interactions with BTLA,CD160 and LIGHT

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Cellular & Molecular Immunology 2019年第7期16卷 679-682页

作者： Jose Ignacio Rodriguez-Barbosa Pascal Schneider Andreas Weigert Kyung-Mi Lee Tae-Jin Kim Jose-Antonio Perez-Simon Maria-Luisa del Rio Transplantation Immunobiology Section Research Institutes of the University of LeonCampus of Vegazana s/n24071 LeonSpain Leon Regional Transplantation Coordination Center Leon University HospitalLeonSpain Acción Estratégica en Salud Consorcio CIBER-ONCSevilleSpain department of Biochemistry University of Lausanne1066 EpalingesSwitzerland Institute of Biochemistry I Faculty of MedicineGoethe-University Frankfurt60590 FrankfurtGermany department of Biochemistry and Molecular Biology Korea University College of MedicineSeoul 02841Republic of Korea department of Biomedical Engineering Center for Bio-Integrated ElectronicsSimpson Querrey Institute for BioNanotechnologyNorthwestern UniversityEvanstonIL 60208USA department of Hematology Virgen del Rocio University HospitalBiomedical Institute of SevillaSevillaSpain

Temporal and spatial expression of cosignaling receptors and their ligands regulates the early stages of T-cell activation(signal 1,T-cell receptor(TCR)signaling and signal 2,costimulation/coinhibition),clonal expansion and T-cell survival during their differentiation towards effector T *** the inflammatory stimulus is eliminated,effector T cells return to homeostasis after undergoing a contraction phase by activation-induced cell death and the intervention of ligands for coinhibitory receptors,leaving a population of long-term memory T *** expression of ligands for coinhibitory receptors on hematopoietic cells and,more importantly,on non-hematopoietic cells of peripheral tissues is a key process in tuning the functional activity of effector T cells to prevent excess tissue inflammation that may lead to immunopathology and subsequent tissue dysfunction.

关键词： stimulation homeostasis inflammation

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GOECP/SEOR radiotherapy guidelines for small-cell lung cancer

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World Journal of Clinical Oncology 2021年第3期12卷 115-143页

作者： Felipe Couñago Carolina de la Pinta Susana Gonzalo Castalia Fernández Piedad Almendros Patricia Calvo Begoña Taboada Antonio Gómez-Caamaño JoséLuis López Guerra Marisa Chust JoséAntonio GonzálezFerreira AnaÁlvarez González Francesc Casas department of Radiation Oncology Hospital Universitario Quirónsalud MadridHospital La LuzUniversidad Europea de MadridMadrid 28223MadridSpain department of Radiation Oncology Hospital Universitario Ramón y CajalMadrid 28034Spain department of Radiation Oncology Hospital Universitario La PrincesaMadrid 28006Spain department of Radiation Oncology GenesisCare MadridMadrid 28043Spain department of Radiation Oncology Hospital General UniversitarioValencia 46014Spain department of Radiation Oncology Hospital Clínico Universitario Santiago de CompostelaSantiago de Compostela 15706Spain department of Radiation Oncology Hospital Universitario Virgen del RocíoSevilla 41013Spain department of Radiation Oncology Fundación Instituto Valenciano de OncologíaValencia 46009Spain department of Radiation Oncology GenesisCare-SpainSevilla 41092Spain department of Radiation Oncology HGU Gregorio MarañónMadrid 28007Spain department of Radiation Oncology Thoracic UnitHospital ClinicBarcelona 08036Spain

Small cell lung cancer(SCLC)accounts for approximately 20%of all lung *** main treatment is chemotherapy(Ch).However,the addition of radiotherapy significantly improves overall survival(OS)in patients with non-metastatic SCLC and in those with metastatic SCLC who respond to *** cranial irradiation reduces the risk of brain metastases and improves OS in both metastatic and non-metastatic *** 5-year OS rate in patients with limited-stage disease(non-metastatic)is slightly higher than 30%,but less than 5%in patients with extensive-stage disease(metastatic).The present clinical guidelines were developed by Spanish radiation oncologists on behalf of the Oncologic Group for the Study of Lung Cancer/Spanish Society of Radiation Oncology to provide a current review of the diagnosis,planning,and treatment of *** guidelines emphasise treatment fields,radiation techniques,fractionation,concomitant treatment,and the optimal timing of Ch and ***,we discuss the main indications for reirradiation in local recurrence.

关键词： Small cell lung cancer Chemotherapy Hyperfractionated radiation therapy Prophylactic brain irradiation Brain metastases Reirradiation

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Simulating FAS-induced apoptosis by using P systems

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Progress in Natural Science:Materials International 2007年第4期17卷 424-431页

作者： Smitha Cheruku Andrei Pǎun Francisco J. Romero-Campero Mario J. Pérez-Jiménez Oscar H. Ibarra department of Computer Science/IfM Louisiana Tech UniversityP.O. Box 10348RustonLA 71272USA department of Computer Science and Artificial Intelligence University of SevilleAvda. Reina Mercedes s/n 41012SevillaSpain department of Computer Science University of California-Santa BarbaraSanta BarbaraCA 93106USA

In contrast to differential equations, P systems are an unconventional model of computation which takes into consideration the discrete character of the quantity of components and the inherent randomness that exists in biological phenomena. The key feature of P systems is their compartmentalised structure which represents the heterogeneity of the structural organisation of the cells, and where one can take into account the role played by membranes in the functioning of the system, for example signalling at the cell surface, selective uptake of substances from the media, diffusion across different compartments, etc. We show here that P systems can be a reliable tool for Systems Biology and could even outperform in some cases the current simulation techniques based on differential equations. We will also use a strategy based on the well known Gillespie algorithm but running on more than one compartment called Multi-compartmental Gillespie Algorithm.

关键词： FAS-induced apoptosis P systems deterministic waiting times algorithm multi-compartmental Gillespie algorithm.

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Hepatobiliary manifestations in inflammatory bowel disease: The gut,the drugs and the liver

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World Journal of Gastroenterology 2013年第42期19卷 7327-7340页

作者： María Rojas-Feria Manuel Castro Emilio Suárez Javier Ampuero Manuel Romero-Gómez Unit for Medical and Surgical Management of Digestive Diseases and CIBERehd Valme University HospitalUniversity of SevilleE-41014 SevillaSpain

Abnormal liver biochemical tests are present in up to30%of patients with inflammatory bowel disease(IBD),and therefore become a diagnostic *** and biliary tract diseases are common extraintestinal manifestations for both Crohn’s disease and ulcerative colitis(UC),and typically do not correlate with intestinal *** sclerosing cholangitis(PSC)is the most common hepatobiliary manifestation of IBD,and is more prevalent in *** 5%of patients with UC develop PSC,with the prevalence reaching up to 90%.Cholangiocarcinoma and colon cancer risks are increased in these *** common disorders include autoimmune hepatitis/PSC overlap syndrome,IgG4-associated cholangiopathy,primary biliary cirrhosis,hepatic amyloidosis,granulomatous hepatitis,cholelithiasis,portal vein thrombosis,liver abscess,and non-alcoholic fatty liver *** B reactivation during immunosuppressive therapy is a major concern,with screening and vaccination being recommended in serologically negative cases for patients with *** prophylaxis with entecavir or tenofovir for 6to 12 mo after the end of immunosuppressive therapy is mandatory in patients showing as hepatitis B surface antigen(HBsAg)positive,independently from viral *** negative and anti-HBc positive patients,with or without anti-HBs,should be closely monitored,measuring alanine aminotransferase and hepatitis B virus DNA within 12 mo after the end of therapy,and should be treated if the viral load *** the other hand,immunosuppressive therapy does not seem to promote reactivation of hepatitis C,and hepatitis C antiviral treatment does not influence IBD natural history *** of the drugs used for IBD treatment may induce hepatotoxicity,although the incidence of serious adverse events is *** in liver biochemical tests associated with aminosalicylates are uncommon and are usually not clinically *** hepatotoxicity has been describ

关键词： Inflammatory bowel disease Hepatobiliary disorders Extraintestinal manifestations Primary sclerosing cholangitis Drug-induced liver injury Hepatotoxicity Hepatitis B Hepatitis C

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Single sperm selection and DNA fragmentation analysis: The case of MSOME/IMSI

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Natural Science 2013年第7期5卷 7-14页

作者： Jaime Gosálvez Beatriz Migueles Carmen López-Fernández Fernando Sanchéz-Martín Pascual Sáchez-Martín department of Biology Genetics Unit University Autónoma of Madrid Madrid Spain Ginemed Clinic República Argentina Av Sevilla Spain

Sperm of poor quality may affect syngamy after fertilization, embryo development up to the blastocyst stage and reproductive outcome. Subsequently, sperm selection based on morphological characteristics and sperm DNA quality may help to partially avoid these problems. Today, highly efficient sperm selection based on morphological characteristics can be attained using the motile sperm organelle morphology (MSOME) examination, and the spermatozoa selected can be used for ICSI through a fertilization strategy known as intra-cytoplasmic morphologically selected sperm injection (IMSI). The aim of this investigation was to develop a simple methodology to assess sperm DNA fragmentation in single spermatozoa following MSOME/ IMSI, to test the hypothesis that morphologically normal spermatozoa, with an absence of vacuolization, is free of DNA damage. The results indicated that MSOME/IMSI-selected sperm, combined with the Sperm Chromatin Dispersion test (SCD;Oligo-Halosperm), can be reliably used to assess sperm DNA damage in selected single spermatozoa (75% average efficiency), thereby establishing a direct relationship between a good morphological pattern on the sperm and a good DNA quality. Furthermore, results showed spermatozoa presenting a normal morphology and no traces of vacuolization to be fully free of DNA damage. However, traces of vacuolization and more severe morphological alterations were accompanied by significant increases in the proportion of sperm containing a damaged DNA molecule. Interestingly, subtle morphological differences observed between normal and non-vacuolated and normal but vacuolated sperm exhibited significant differrences in the ability of the SCD-Oligo-Halosperm treated sperm to expand DNA fibers following protein depletion.

关键词： MSOME/IMSI Assisted Reproductive Technology Fertility Sperm DNA Fragmentation Male Factor Semen Processing

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A Script-Based Prototyping Framework to Boost Agile-UX developments

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Journal of Computer Science & Technology 2016年第6期31卷 1246-1261页

作者： Pedro Luis Mateo Navarro Gregorio Martinez Perez Diego sevilla Ruiz Catedra Sociedad Anonima de Electrdnica Submarina ( SAES) Laboratories University of Murcia Murcia 30100 Spain

Prototypes are described as a successful mechanism to incorporate user-experience design （UX） into Agile de- velopments, but their integration into such developments is not exempt from difficulties. Prototypes and final applications are often developed using different tools, which hinders the collaboration between designers and developers and also compli- cates reuse. Moreover, integrating stakeholders such as clients and users into the Agile process of designing, evaluating, and refining a prototype is not straightforward mainly because of its iterative nature. In an attempt to tackle these problems, this work presents the design and implementation of a new framework in which scripting languages are used to code prototyped behaviors. Prototyping is then treated as a separate aspect that coexists and runs together with final functionality. Using this framework communication is enhanced because designers and developers work in parallel on the same software artifact. Prototypes are fully reused and iteratively added with final functionality while prototyped behaviors are removed. They can be also modified on the fly to implement participatory design techniques.

关键词： prototype Agile development aspect-oriented development scripting language participatory design

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deep diving in the PACIFIC:Practical issues in stage III non-small cell lung cancer to avoid shipwreck

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World Journal of Clinical Oncology 2020年第11期11卷 898-917,I0003页

作者： Xabier Mielgo-Rubio Federico Rojo Laura Mezquita-Pérez Francesc Casas Amadeo Wals Manel Juan Carlos Aguado Javier Garde-Noguera David Vicente Felipe Couñago department of Medical Oncology Hospital Universitario Fundación AlcorcónMadrid 28922Spain department of Pathology IIS-Jiménez Díaz-CIBERONC FoundationMadrid 28040Spain department of Medical Oncology Hospital ClinicLaboratory of Translational Genomics and Targeted Therapeutics in Solid TumorsIDIBAPSBarcelona 08036Spain department of Radiation Oncology Hospital ClinicBarcelona 08036Spain department of Radiation Oncology Hospital Universitario Virgen MacarenaSevilla 41009Spain department of Immunology Service Hospital ClínicUniversitat de BarcelonaBarcelona 08036Spain department of Medical Oncology Hospital Universitario Clínico San CarlosMadrid 28040Spain department of Medical Oncology Hospital Universitario Virgen MacarenaSevilla 49001Spain department of Radiation Oncology Hospital Universitario Quirónsalud MadridHospital La LuzUniversidad Europea de MadridMadrid 28028Spain

After publication of the PACIFIC trial results,immune checkpoint inhibitor-based immunotherapy was included in the treatment algorithm of locally advanced non-small cell lung cancer(NSCLC).The PACIFIC trial demonstrated that 12 mo of durvalumab consolidation therapy after radical-intent platinum doublet chemotherapy with concomitant radiotherapy improved both progression-free survival and overall survival in patients with unresectable stage III *** is the first treatment in decades to successfully improve survival in this clinical setting,with manageable toxicity and without deterioration in quality of *** integration of durvalumab in the management of locally advanced NSCLC accentuates the need for multidisciplinary,coordinated decision-making among lung cancer specialists,bringing new challenges and controversies as well as important changes in clinical work *** aim of the present article is to review—from a practical,multidisciplinary perspective—the findings and implications of the PACIFIC *** evaluate the immunobiological basis of durvalumab as well as practical aspects related to programmed cell death ligand 1 *** addition,we comprehensively assess the efficacy and toxicity data from the PACIFIC trial and discuss the controversies and practical aspects of incorporating durvalumab into routine clinical ***,we discuss unresolved questions and future *** short,the present document aims to provide clinicians with a practical guide for the application of the PACIFIC regimen in routine clinical practice.

关键词： Durvalumab Non-small cell lung cancer PACIFIC Immunotherapy Immune checkpoint inhibitors Anti-programmed cell death ligand 1 Consolidation therapy Unresectable stage III lung cancer

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