检索结果-南通市图书馆

Self-assembling protein nanocarrier for selective delivery of cytotoxic polypeptides to CXCR4^(+) head and neck squamous cell carcinoma tumors

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第5期12卷 2578-2591页

作者： Elisa Rioja-Blanco Irene Arroyo-Solera Patriciaálamo Isolda Casanova Alberto Gallardo Ugutz Unzueta Naroa Serna Laura Sánchez-García Miquel Quer Antonio Villaverde Esther Vázquez Ramon Mangues Lorena Alba-Castellón Xavier León Institut d'InvestigacióBiomèdica Sant Pau(IIB-Sant Pau) Barcelona 08041Spain Institut de Recerca contra la Leucèmia Josep Carreras Barcelona 08025Spain ciber de bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid 28029Spain department of Pathology Hospital de la Santa Creu i Sant PauBarcelona 08041Spain Institut de Biotecnologia i de Biomedicina Universitat Autònoma de BarcelonaBellaterra 08193Spain departament de Genètica i de Microbiologia Universitat Autònoma de BarcelonaBellaterra 08193Spain department of Otorhinolaryngology Hospital de la Santa Creu i Sant PauBarcelona 08041Spain

Loco-regional recurrences and distant metastases represent the main cause of head and neck squamous cell carcinoma(HNSCC) mortality. The overexpression of chemokine receptor 4(CXCR4) in HNSCC primary tumors associates with higher risk of developing loco-regional recurrences and distant metastases, thus making CXCR4 an ideal entry pathway for targeted drug delivery. In this context, our group has generated the self-assembling protein nanocarrier T22-GFP-H6, displaying multiple T22 peptidic ligands that specifically target CXCR4. This study aimed to validate T22-GFP-H6 as a suitable nanocarrier to selectively deliver cytotoxic agents to CXCR4^(+)tumors in a HNSCC model. Here we demonstrate that T22-GFP-H6 selectively internalizes in CXCR4^(+)HNSCC cells, achieving a high accumulation in CXCR4^(+)tumors in vivo, while showing negligible nanocarrier distribution in non-tumor bearing organs. Moreover, this T22-empowered nanocarrier can incorporate bacterial toxin domains to generate therapeutic nanotoxins that induce cell death in CXCR4-overexpressing tumors in the absence of histological alterations in normal organs. Altogether, these results show the potential use of this T22-empowered nanocarrier platform to incorporate polypeptidic domains of choice to selectively eliminate CXCR4^(+)cells in HNSCC. Remarkably, to our knowledge, this is the first study testing targeted proteinonly nanoparticles in this cancer type, which may represent a novel treatment approach for HNSCC patients.

关键词： Targeted drug delivery Protein nanoparticles CXCR4 receptor HNSCC Cell targeting Recombinant proteins Nanotoxins Cancer therapy

Structural and biological engineering of 3D hydrogels for wound healing

在线全文

学校读者我要写书评

暂无评论

生物活性材料(英文) 2023年第6期24卷 197-235页

作者： Mohammad Hadi Norahan Sara Cristina Pedroza-González Mónica Gabriela Sánchez-Salazar Mario Moisés Álvarez Grissel Trujillo de Santiago Escuela de Ingeniería y Ciencias Tecnológico de MonterreyMonterreyNL64849Mexico Centro de Biotecnología-FEMSA Tecnológico de MonterreyMonterreyNuevo LeónCP64849Mexico departamento de bioingeniería Tecnológico de MonterreyMonterreyNuevo LeónCP64849Mexico

Chronic wounds have become one of the most important issues for healthcare systems and are a leading cause of death worldwide.Wound dressings are necessary to facilitate wound treatment.Engineering wound dressings may substantially reduce healing time,reduce the risk of recurrent infections,and reduce the disability and costs associated.In the path of engineering of an ideal wound dressing,hydrogels have played a leading role.Hydrogels are 3D hydrophilic polymeric structures that can provide a protective barrier,mimic the native extracellular matrix(ECM),and provide a humid environment.Due to their advantages,hydrogels(with different architectural,physical,mechanical,and biological properties)have been extensively explored as wound dressing platforms.Here we describe recent studies on hydrogels for wound healing applications with a strong focus on the interplay between the fabrication method used and the architectural,mechanical,and biological performance achieved.Moreover,we review different categories of additives which can enhance wound regeneration using 3D hydrogel dressings.Hydrogel engineering for wound healing applications promises the generation of smart solutions to solve this pressing problem,enabling key functionalities such as bacterial growth inhibition,enhanced re-epithelialization,vascularization,improved recovery of the tissue functionality,and overall,accelerated and effective wound healing.

关键词： Wound healing Wound dressing Hydrogel Tissue engineering 3D culture

Site-directed cysteine coupling of disulfide-containing non-antibody carrier proteins(THIOCAPs)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Materials 2023年第10期66卷 4109-4120页

作者： Ariana Rueda Julian I.Mendoza Lorena Alba-Castellon Eloi Parladé Eric Voltà-Durán David Paez Anna Avino Ramon Eritja Esther Vázquez Antonio Villaverde Ramón Mangues Ugutz Unzueta Institut d'Investigació Biomèdica Sant Pau(IIB Sant Pau) Sant Quintí 77-7908041 BarcelonaSpain Josep Carreras Leukaemia Research Institute(IJC Campus Sant Pau) 08041 BarcelonaSpain ciber de bioingeniería Biomateriales y NanomedicinaInstituto de Salud Carlos Ⅲ28029 MadridSpain Institut de Biotecnologia i de Biomedicina Universitat Autònoma de Barcelona08193 BellaterraSpain departament de Genètica i de Microbiologia Universitat Autònoma de Barcelona08193 BellaterraSpain department of Medical Oncology Hospital de la Santa Creu i Sant Pau.Barcelona08025 BarcelonaSpain ciber de Enfermedades Raras(ciberER) Instituto de Salud Carlos Ⅲ28029 MadridSpain Institute for Advanced Chemistry of Catalonia(IQAC) CSIC08034 BarcelonaSpain

The development of a new generation of nonantibody protein drug delivery systems requires site-directed conjugation strategies to produce homogeneous,reproducible and scalable nanomedicines.For that,the genetic addition of cysteine residues into solvent-exposed positions allows the thiol-mediated cysteine coupling of therapeutic drugs into protein-based nanocarriers.However,the high reactivity of unpaired cysteine residues usually reduces protein stability,consequently imposing the use of more methodologically demanding purification procedures.This is especially relevant for disulfide-containing nanocarriers,as previously observed in THIOMABs.Moreover,although many protein scaffolds and targeting ligands are also rich in disulfide bridges,the use of these methodologies over emerging non-antibody carrier proteins has been completely neglected.Here,we report the development of a simple and straightforward procedure for a onestep production and site-directed cysteine conjugation of disulfide-containing non-antibody thiolated carrier proteins(THIOCAPs).This method is validated in a fluorescent C-X-C chemokine receptor 4(CXCR4)-targeted multivalent nanocarrier containing two intramolecular disulfide bridges and one reactive cysteine residue strategically placed into a solvent-exposed position(THIO-T22-GFP-H6)for drug conjugation and in a humanized alternative intended for clinical applications(T22-HSNBT-H6).Thus,we produce very stable,homogeneous and fully functional antitumoral nanoconjugates(THIO-T22-GFP-H6-MMAE and T22-HSNBT-H6-MMAE)that selectively eliminate target cancer cells via CXCR4-receptor.Altogether,the developed methodology appears as a powerful tool for the rational engineering of emerging non-antibody,cell-targeted protein nanocarriers that contain disulfide bridges together with a solvent-exposed reactive cysteine(THIOCAP).This should pave the way for the development of a new generation of stable,homogeneous and efficient nanomedicines.

关键词： disulfide directed bridges

The spectrum of building block conformers sustains the biophysical properties of clinically-oriented self-assembling protein nanoparticles

在线全文

学校读者我要写书评

暂无评论

Science China Materials 2022年第6期65卷 1662-1670页

作者： Eric Voltà-Durán Julieta M Sánchez Hèctor-López-Laguna Eloi Parladé Laura Sánchez-García Alejandro Sánchez-Chardi Ario de Marco Ugutz Unzueta Esther Vázquez Antonio Villaverde Institut de Biotecnologia i de Biomedicina Universitat Autònoma de BarcelonaBellaterra 08193BarcelonaSpain departament de Genètica i de Microbiologia Universitat Autònoma de BarcelonaBellaterra 08193BarcelonaSpain ciber de bioingeniería Biomateriales y Nanomedicina(ciber-BBN) Bellaterra 08193BarcelonaSpain Instituto de Investigaciones Biológicas y Tecnológicas(IIBYT)(CONICET-Universidad Nacional de Córdoba) ICTA&Cátedra de Química BiológicaDepartamento de QuímicaFCEFyNUNC.Av.Velez Sarsfield 1611X 5016GCA CórdobaArgentina Servei de Microscòpia Universitat Autònoma de BarcelonaBellaterra 08193BarcelonaSpain departament de Biologia Evolutiva Ecologia i Ciències AmbientalsFacultat de BiologiaUniversitat de Barcelona08028 BarcelonaSpain Laboratory for Environmental and Life Sciences University of Nova GoricaNova Gorica 5000Slovenia Biomedical Research Institute Sant Pau(IIB Sant Pau) Sant Antoni Ma Claret 16708025 BarcelonaSpain

Histidine-rich peptides confer self-assembling properties to recombinant proteins through the supramolecular coordination with divalent cations.This fact allows the cost-effective,large-scale generation of microscopic and macroscopic protein materials with intriguing biomedical properties.Among such materials,resulting from the simple bioproduction of protein building blocks,homomeric nanoparticles are of special value as multivalent interactors and drug carriers.Interestingly,we have here identified that the assembly of a given His-tagged protein might render distinguishable categories of self-assembling protein nanoparticles.This fact has been scrutinized through the nanobody-containing fusion proteins EM1-GFP-H6 and A3C8-GFP-H6,whose biosynthesis results in two distinguishable populations of building blocks.In one of them,the assembling and disassembling is controllable by cations.However,a second population immediately self-assembles upon purification through a non-regulatable pathway,rendering larger nanoparticles with specific biological properties.The structural analyses of both model proteins and nanoparticles revealed important conformational variability in the building blocks.This fact renders different structural and functional categories of the final soft materials resulting from the participation of energetically unstable intermediates in the oligomerization process.These data illustrate the complexity of the Hismediated protein assembling in recombinant proteins but they also offer clues for a better design and refinement of protein-based nanomedicines,which,resulting from biological fabrication,show an architectonic flexibility unusual among biomaterials.

关键词： recombinant proteins self-assembling protein materials nanoparticles biomaterials

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence antiquenching

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2022年第4期12卷 637-644页

作者： Lingyuan Xu Xiuyuan Zhang A.M.Abd El-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvador Ahmet Hacimuftuoglu Xueyan Cui Yudan Zhang Kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin Bruce D.Hammock Institute of Quality Standard and Testing Technology for Agro-Products Chinese Academy of Agricultural SciencesBeijing100081China department of Pharmacology Faculty of Veterinary MedicineCairo UniversityGiza12211Egypt department of Medical Pharmacology Medical FacultyAtaturk UniversityErzurum25240Türkiye Institute of Livestock and Poultry Ningbo Academy of Agricultural SciencesNingboZhejiang315040China Nanobiotechnology for Diagnostics Group Instituto de Química Avanzada de CatalunaIQAC-CSICBarcelona08034Spain ciber de bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid28029Spain Inspection and Testing Center of Agricultural and Livestock Products of Tibet Department of Agriculture and Rural Affairs of Tibet Autonomous RegionLhasa850000China department of Entomology&Nematology and the UC Davis Comprehensive Cancer Center University of CaliforniaDavisCA95616USA

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and rice.Gold nanoparticles were functionalized with monoclonal antibodies against the tested OPs.DNA oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal amplification.The detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the fluorophore.The resulting fluorescence signal enables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concentration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,respectively.The mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

关键词： Organophosphate pesticides Fluorescence anti-quenching Gold nanoparticles DNA-RNA hybridization DNA oligonucleotides Ribonuclease H

Hydrogels for neuroprotection and functional rewiring: a new era for brain engineering

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2020年第5期15卷 783-789页

作者： Rocío Fernández-Serra Rebeca Gallego Paloma Lozano Daniel González-Nieto Center for Biomedical Technology Universidad Politécnica de Madrid. MadridSpain departamento de Tecnología Fotónica y bioingeniería ETSI TelecomunicacionesUniversidad Politécnica de MadridMadridSpain Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (ciber-BBN) MadridSpain

The neurological devastation of neurodegenerative and cerebrovascular diseases reinforces our perseverance to find advanced treatments to deal with these fatal pathologies.High-performance preclinical results have failed at clinical level,as it has been the case for a wide variety of neuroprotective agents and cell-based therapies employed to treat high prevalent brain pathologies such as stroke,Alzheimer’s and Parkinson’s diseases.An unquestionable reality is the current absence of effective therapies to neuroprotect the brain,to arrest neurodegeneration and rewire the impaired brain circuits.Part of the problem might arise from the lack of adequate in vitro and in vivo models and that most of the underlying pathophysiological mechanisms are not yet clarified.Another contributing factor is the lack of efficient systems to sustain drug release at therapeutic concentrations and enhance the survival and function of grafted cells in transplantation procedures.For medical applications the use of biomaterials of different compositions and formats has experienced a boom in the last decades.Although the greater complexity of central nervous system has probably conditioned their extensive use with respect to other organs,the number of biomaterials-based applications to treat the injured brain or in the process of being damaged has grown exponentially.Hydrogel-based biomaterials have constituted a turning point in the treatment of cerebral disorders using a new form of advanced therapy.Hydrogels show mechanical properties in the range of cerebral tissue resulting very suitable for local implantation of drugs and cells.It is also possible to fabricate three-dimensional hydrogel constructs with adaptable mesh size to facilitate axonal guidance and elongation.Along this article,we review the current trends in this area highlighting the positive impact of hydrogel-based biomaterials over the exhaustive control of drug delivery,cell engraftment and axonal reinnervation in brain pathologies.

关键词： advanced therapies Alzheimer’s disease biomaterials brain hydrogels neurological diseases Parkinson’s disease polymers stroke

Anchor Root development: A World within Worlds

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2020年第8期13卷 1105-1107页

作者： JoséManuel Pérez-Pérez Instituto de bioingeniería Universidad Miguel Hernández03202 ElcheSpain

Many plant species develop shoot-borne adventitious roots(ARs)with specialized functions that contribute to plant adaptability by enhancing resource capture and stress avoidance.In Arabidopsis,as in many dicots,the postembryonic root system is primarily composed of lateral roots(LRs),which originate from the xylem pole pericycle cells through a process that involves periodic oscillation in gene expression mediated by a β-carotenoidderived signal(Dickinson et al.,2019).Our understanding of the key molecular and signaling events regulating AR initiation has recently been obtained from studies in Arabidopsis,where ARs are formed on etiolated hypocotyls upon transfer to light(Lakehal et al.,2019)or from excised leaf explants(Zhang et al.,2019).Anchor roots(ANRs)are a type of AR that originate immediately below the root-hypocotyl junction in an auxindependent manner and that require a diapocarotenoid-derived signal(anchorene)to emerge(Jia et al.,2019).ANR primordia activation may represent a generic mechanism to compensate for the loss of the growth potential of the primary root(PR),as it is enhanced by mutations affecting PR growth,by whole-root excision or by nitrogen deficiency(Jia et al.,2019).A recent paper by Bai et al.(2020)provides direct evidence that a regulatory module involving the AP2/ERF transcription factor ABSCISIC ACID INSENSITIVE 4(ABI4),the mitogen-activated protein kinases(MAPKs)MPK3 and MPK6,and the phosphatase PP2C12 controls ANR emergence in Arabidopsis.

关键词： roots Arabidopsis Root

2D organic molecular metallic soft material derived from BEDO-TTF with electrochromic and rectifying properties

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

npj Flexible Electronics 2018年第1期2卷 237-243页

作者： Daniel Suarez Eden Steven Elena Laukhina Andres Gomez Anna Crespi Narcis Mestres Concepcio Rovira Eun Sang Choi Jaume Veciana department of Physics National High Magnetic Field Laboratory Florida State University1800 East Paul Dirac DriveTallahasseeFL 32310USA Emmerich Education Center Jakarta 14450Indonesia ciber de bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)MadridSpain Institut de Ciencia de Materials de Barcelona(ICMAB-CSIC) Campus UABBellaterra 08193Spain

In this article we demonstrate that a migration of iodine species and chemical transformation in a moist environment induced by a voltage-biased Pt electrode is able to alter the color and degree of charge transfer in a layer of the 2D organic molecular metal(BEDO-TTF)_(2.4)I_(3)[BEDO=bis(ethylenedioxy)tetrathiafulvalene]self-assembled at the surface of a polycarbonate film.These effects produce a reversible electrochromic behavior of the layer with low operating voltages and fast operation times.Adjuvant with electrochromism,this flexible material exhibits rectifying behavior whose I-V curves are dependent on the voltage sweep directions.These results open new possibilities for the design and fabrication of organic flexible materials for soft electrochromic and rectifying components.The easy working principle ensures reliability,low power consumption,and versatility through its implementation into simple devices.Such working principle has been confirmed by temperature dependent resistance measurements,X-Ray,EDX-SEM,and conducting-AFM studies.

关键词： resistance metallic behavior

Getting started in mapping-by-sequencing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2015年第7期57卷 606-612页

作者： Héctor Candela Rubén Casanova-Sáez José Luis Micol Instituto de bioingeniería Universidad Miguel Hernández department of Forest Genetics and Plant Physiology Umea Plant Science CenterSwedish University of Agricultural Sciences

Next-generation sequencing （NGS） technologies allow the cost-effective sequencing of whole genomes and have expanded the scope of genomics to novel applications, such as the genome-wide characterization of intraspecific polymorphisms and the rapid mapping and identification of point mutations. Next-generation sequencing platforms, such as the Illumina HiSeq2ooo platform, are now commercially available at affordable prices and routinely produce an enormous amount of sequence data, but their wide use is often hindered by a lack of knowledge on how to manipulateand process the information produced. In this review, we focus on the strategies that are available to geneticists who wish to incorporate these novel approaches into their research but who are not familiar with the necessary bioinformatic concepts and computational tools. In particular, we comprehensively summarize case studies where the use of NGS technologies has led to the identification of point mutations, a strategy that has been dubbed ＂mapping-by-sequencing＇, and review examples from plants and other model species such as Caenorhabditis elegans, Saccharomyces cerevisiae, and Drosophila mela- nogaster. As these technologies are becoming cheaper and more powerful, their use is also expanding to allow mutation identification in species with larger genomes, such as many crop plants.

关键词： Mapping-by-sequencing massively parallel sequencing mutation identification SHOREmapping whole-genome re-sequencing