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Interpenetrated Magnesium-Tricalcium Phosphate Composite:Manufacture,Characterization and In Vitro Degradation Test

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Acta Metallurgica Sinica(English Letters) 2017年第4期30卷 319-325页

作者： Mariano Casas-Luna Serhii Tkachenko Miroslava Horynova Lenka Klakurkova Pavel Gejdos Sebastian Diaz-de-la-Torre Ladislav Celko Jozef Kaiser Edgar B. Montufar ceitec-Central European Institute of Technology Brno University of TechnologyPurkyfnova 656/123612 00 BmoCzech Republic CIITEC-Centro de Investigacion e Innovacion Tecnologica Instituto Politocnico NacionalCerrada de CECATI02250 Mexico CityMexico

Magnesium and calcium phosphates composites are promising biomaterials to create biodegradable load-bearing implants for bone regeneration. The present investigation is focused on the design of an interpenetrated magnesium- tricalcium phosphate （Mg-TCP） composite and its evaluation under immersion test. In the study, TCP porous preforms were fabricated by robocasting to have a prefect control of porosity and pore size and later infiltrated with pure commercial Mg through current-assisted metal infiltration （CAMI） technique. The microstructure, composition, distribution of phases and degradation of the composite under physiological simulated conditions were analysed by scanning electron microscopy, elemental chemical analysis and X-ray diffraction. The results revealed that robocast TCP preforms were full infiltrated by magnesium through CAMI, even small pores below 2 μm have been filled with Mg, giving to the composite a good interpenetration. The degradation rate of the Mg-TCP composite displays lower value compared to the one of pure Mg during the first 24 h of immersion test.

关键词： Calcium phosphate Magnesium Liquid metal infiltration Spark plasma sintering Corrosion

ABP1： Finally Docking

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Molecular Plant 2015年第3期8卷 356-358页

作者： Peter Grones Institute of Science and Technology (IST) Austria 3400 Klosterneuburg Austria Department of Plant Systems Biology VIB and Department of PlantBiotechnology and Bioinformatics Ghent University 9052 Gent Belgium Mendel Centre for Plant Genomics and Proteomics Masaryk University (CEITEC MU) 62500 Brno Czech Republic

Auxin, one of the plant hormones, is a key regulator of plant growth and development. At the cellular level, it controls different processes, such as cell expansion, division, and differentiation that are reflected by its regulatory role in a plethora of developmental mechanisms. An important feature of the auxin action is its differential distribution within tissues mediated by the polar auxin transport machinery, which can be dynamically regulated in response to internal and external stimuli. Receptors at the cell surface or cell interior are needed to sense and interpret fluctuations in the auxin distribution.

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High temperature increases centromeremediated genome elimination frequency and enhances haploid induction in Arabidopsis

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Plant Communications 2023年第3期4卷 126-138页

作者： Ulkar Ahmadli Manikandan Kalidass Lucie Crhak Khaitova Joerg Fuchs Maria Cuacos Dmitri Demidov Sheng Zuo Jana Pecinkova Martin Mascher Mathieu Ingouff Stefan Heckmann Andreas Houben Karel Riha Inna Lermontova Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben Corrensstrasse 306466 SeelandGermany Central European Institute of Technology(ceitec)and National Centre for Biomolecular Research Faculty of ScienceMasaryk UniversityKamenice 562500 BrnoCzech Republic CIRAD DIADEIRDUniversity of Montpellier34393 MontpellierFrance

Double haploid production is the most effective way to create true-breeding lines in a single *** Arabidopsis,haploid induction via mutation of the centromere-specific histone H3(cenH3)has been shown when the mutant is outcrossed to the wild-type,and the wild-type genome remains in the haploid ***,factors that affect haploid induction are still poorly ***,we report that a mutant of the cenH3 assembly factor Kinetochore Null2(KNL2)can be used as a haploid inducer when pollinated by the *** discovered that short-term temperature stress of the knl2 mutant increased the efficiency of haploid induction *** also demonstrated that a point mutation in the CENPC-k motif of KNL2 is sufficient to generate haploid-inducing lines,suggesting that haploidinducing lines in crops can be identified in a naturally occurring or chemically induced mutant population,avoiding the generic modification(GM)approach at any ***,a cenh3-4 mutant functioned as a haploid inducer in response to short-term heat stress,even though it did not induce haploids under standard ***,we identified KNL2 as a new target gene for the generation of haploid-inducer lines and showed that exposure of centromeric protein mutants to high temperature strongly increases their haploid induction efficiency.

关键词： centromere kinetochore null 2 CENPC-k cenh3-4 temperature stress haploid induction

High-resolution characterization of hexagonal boron nitride coatings exposed to aqueous and air oxidative environments

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Nano Research 2017年第6期10卷 2046-2055页

作者： Lanlan Jiang Na Xiao Bingru Wang Enric Grustan-Gutierrez Xu Jing Petr Babor Miroslav Kogbal Guangyuan Lu Tianru Wu Haomin Wang Fei Hui Yuanyuan Shi Bo Song Xiaoming Xie Mario Lanza Institute of Functional Nano & Soft Materials (FUNSOM) Soochow University Suzhou 215123 China ceitec but Brno University of Technology Brno 61669 Czech Republic State Key Laboratory of Functional Materials for In formatics Shanghai Institute of Microsystems and Information Technology Chinese Academy of Sciences Shanghai 200050 China School of Physical Science and Technology Shanghai Tech University Shanghai 201210 China

Hexagonal boron nitride （h-BN） is believed to offer better passivation to metallic surfaces than graphene owing to its insulating nature, which facilitates blocking the flow of electrons, thereby preventing the occurrence of galvanic reactions. Nevertheless, this may not be the case when an h-BN-protected material is exposed to aqueous environments. In this work, we analyzed the stability of mono and multilayer h-BN stacks exposed to H202 and atmospheric conditions. Our experiments revealed that monolayer h-BN is as inefficient as graphene as a protective coating when exposed to H202. Multilayer h-BN offered a good degree of protection. Monolayer h-BN was found to be ineffective in an air atmosphere as well. Even a 10-15 layers-thick h-BN stack could not completely protect the surface of the metal under consideration. By combining Auger electron spectroscopy and secondary ion mass spectrometry techniques, we observed that oxygen could diffuse through the grain boundaries of the h-BN stack to reach the metallic substrate. Fortunately, because of the diffusive nature of the process, the oxidized area did not increase with time once a saturated state was reached. This makes multflayer （not monolayer） h-BN a suitable long-term oxidation barrier. Oxygen infiltration could not be observed by X-ray photoelectron spectroscopy. This technique cannot assess the chemical composition of the deeper layers of a material. Hence, the previous reports, which relied on XPS to analyze the passivating properties of h-BN and graphene, may have ignored some important subsurface phenomena. The results obtained in this study provide new insights into the passivating properties of mono and multilayer h-BN in aqueous media and the degradation kinetics of h-BN-coated metals exposed to an air environment.

关键词： hexagonal boron nitride protective coating local oxidation nanoscale two-dimensional (2D)materials

Enquiry into the Topology of Plasma Membrane- Localized PIN Auxin Transport Components

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Molecular Plant 2016年第11期9卷 1504-1519页

作者： Tomasz Nodzynski Steffen Vanneste Marta Zwiewka Marketa Pernisova Jan Hejatko Jiri Friml ceitec - Central European Institute of Technology Masaryk University Kamenice 5 62500 Brno Czech Republic Department of Plant Systems Biology VIB 9052 Gent Belgium Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium National Centre for Biomolecular Research Masaryk University Kamenice 5 62500 Brno Czech Republic Institute of Science and Technology Austria (IST Austria) Am Campus 1 3400 Klosterneuburg Austria

Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regard- less of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immuno- localization techniques, we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane （TM） bundles of five m-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure-function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.

关键词： plasma membrane protein topology auxin efflux carriers Arabidopsis thaliana

Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana

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Molecular Plant 2015年第8期8卷 1175-1187页

作者： Marta Zwiewka Tomasz Nodzynski Stephanie Robert Steffen Vanneste Jiri Friml Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology (CEITEC) Masaryk University Kamenice 5 CZ-625 00 BrnoCzech Republic Department of Plant Systems Biology VlB and Department of Plant Biotechnology and Bioinformatics Ghent University Technologiepark 927 9052 Gent Belgium Institute of Science and Technology (IST) Austria Am Campus 1 3400 Klosterneuburg Austria Present address: Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences/Ume~. Plant Science Center 901 83 UmeaSweden

The sessile life style of plants creates the need to deal with an often adverse environment, in which water availability can change on a daily basis, challenging the cellular physiology and integrity. Changes in os- motic conditions disrupt the equilibrium of the plasma membrane： hypoosmotic conditions increase and hyperosmotic environment decrease the cell volume. Here, we show that short-term extracellular osmotic treatments are closely followed by a shift in the balance between endocytosis and exocytosis in root mer- istem cells. Acute hyperosmotic treatments （ionic and nonionic） enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis, whereas hypoosmotic treatments have the opposite effects. In addition to clathrin recruitment to the plasma membrane, components of early endocytic trafficking are essential during hyperosmotic stress responses. Consequently, growth of seedlings defective in elements of clathrin or early endocytic machinery is more sensitive to hyperosmotic treatments. We also found that the endocytotic response to a change of osmotic status in the environment is dominant over the presum- ably evolutionary more recent regulatory effect of plant hormones, such as auxin. These results imply that osmotic perturbation influences the balance between endocytosis and exocytosis acting through clathrin- mediated endocytosis. We propose that tension on the plasma membrane determines the addition or removal of membranes at the cell surface, thus preserving cell integrity.

关键词： osmotic stress protein trafficking clathrin-mediated endocytosis auxin

Structure,magnetic and adsorption properties of novel FePt/h-BN heteromaterials

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Nano Research 2023年第1期16卷 1473-1481页

作者： Anton S.Konopatsky Vladislava V.Kalinina Alena S.Savchenko Denis.V.Leybo Ekaterina V.Sukhanova Viktor S.Baidyshev Zakhar I.Popov Andrey V.Bondarev Josef Polčák Dmitry V.Shtansky National University of Science and Technology(MISIS) Leninskiy prospect 4Moscow119049Russia Emanuel Institute of Biochemical Physics RAS Kosygina 4Moscow119334Russia Katanov Khakas State University 90 Lenin pr.655017 AbakanRussia Plekhanov Russian University of Economics 36 Stremyanny per.Moscow 117997Russia Department of Control Engineering Faculty of Electrical EngineeringCzech Technical University in PragueTechnicka 2Prague 16627Czech Republic ceitec-Central European Institute of Technology Brno University of TechnologyPurkyňova 123Brno 61200 BrnoCzech Republic

Nanomaterials with high specific surface area and high absorption capacity are attracting increased interest aimed at imparting the desired magnetic *** work is devoted to the study of the effect of heat treatment in a hydrogen atmosphere on the microstructure,adsorption and magnetic properties of heterogeneous FePt/h-BN *** via the polyol process,FePt nanoparticles(NPs)had a size<2 nm and were uniformly distributed over the surface of hexagonal boron nitride(h-BN)*** temperature-activated fcc→fct phase transformation in ultrafine FePt NPs has been well *** NPs act as active centers dissociating H2 molecules and transfer adsorbed hydrogen atoms to the *** functional theory(DFT)calculations also indicate that the h-BN substrate can absorb hydrogen adsorbed on the FePt *** hydrogen circulation in the FePt/h-BN system promoted the fcc→fct phase transformation and allowed to control the magnetic ***/h-BN nanomaterials also exhibited a high adsorption capacity with respect to various organic dyes.

关键词： FePt/h-BN nanomaterials polyol synthesis phase transformations magnetic properties adsorption

Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment （PVC） Function in Arabidopsis

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Molecular Plant 2013年第6期6卷 1849-1862页

作者： Tomasz Nodzynski Mugurel I. Ferarub Sibylle Hirsch Riet De Rycke Claudiu Niculaes Wout Boerjan Jelle Van Leene Geert De Jaeger Steffen Vanneste Jiri Friml Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology (CEITEC) Masaryk University (MU) Kamenice 5 CZ-625 00Brno Czech Republic Department of Plant Systems Biology VIB and Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium Institute of Science and Technology Austria (IST Austria) Am Campus 1 3400 Klosterneuburg Austria Present address: Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences (BOKU) 1190 Vienna Austria Present address: Institute of Plant Biology University of Zurich 8008 ZCirich Switzerland

Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 （pat3） mutant that we identified by an epifluorescence-based for- ward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1-GFR While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compart- ments （PVC） with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A （VPS35A）, a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lyric vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting.

关键词： retromer VPS35 VPS29 prevacuolar compartment (PVC) vacuolar trafficking Arabidopsis thaliana.

A sensing mechanism for the detection of carbon nanotubes using selective photoluminescent probes based on ionic complexes with organic dyes

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Light(Science & Applications) 2016年第1期5卷 686-694页

作者： Petro Lutsyk Raz Arif Jan Hruby Anatolii Bukivskyi Olexander Vinijchuk Mykola Shandura Viktor Yakubovskyi Yuri Kovtun Graham A Rance Michael Fay Yuri Piryatinski Oleksiy Kachkovsky Anatoli Verbitsky Aleksey Rozhin Institute of Physics National Academy of Sciences of Ukraine46prospekt Nauky03680 KyivUkraine School of Engineering&Applied Science Aston UniversityAston TriangleB47ET BirminghamUK Physics Department Faculty of ScienceUniversity of SulaimaniP.O.Box 334SulaimaniIraq-Kurdistan Region Brno University of Technology CEITEC BUTTechnicka´3058/1061600 BrnoCzech Republic Institute of Organic Chemistry National Academy of Sciences of Ukraine5 Murmanska str.02660 KyivUkraine School of Chemistry University of NottinghamUniversity ParkNottinghamNG72RDUK Nottingham Nanotechnology and Nanoscience Centre University of NottinghamUniversity ParkNottinghamNG72RDUK

The multifunctional properties of carbon nanotubes(CNTs)make them a powerful platform for unprecedented innovations in a variety of practical *** a result of the surging growth of nanotechnology,nanotubes present a potential problem as an environmental pollutant,and as such,an efficient method for their rapid detection must be ***,we propose a novel type of ionic sensor complex for detecting CNTs–an organic dye that responds sensitively and selectively to CNTs with a photoluminescent *** complexes are formed through Coulomb attractions between dye molecules with uncompensated charges and CNTs covered with an ionic surfactant in *** demonstrate that the photoluminescent excitation of the dye can be transferred to the nanotubes,resulting in selective and strong amplification(up to a factor of 6)of the light emission from the excitonic levels of CNTs in the near-infrared spectral range,as experimentally observed via excitation-emission photoluminescence(PL)*** chirality of the nanotubes and the type of ionic surfactant used to disperse the nanotubes both strongly affect the amplification;thus,the complexation provides sensing selectivity towards specific ***,neither similar uncharged dyes nor CNTs covered with neutral surfactant form such *** model organic molecules,we use a family of polymethine dyes with an easily tailorable molecular structure and,consequently,tunable absorbance and PL *** provides us with a versatile tool for the controllable photonic and electronic engineering of an efficient probe for CNT detection.

关键词： carbon nanotubes ionic surfactant organic dye photoluminescence sensor