检索结果-南通市图书馆

Open Journal of Pathology 2023年第4期13卷 177-183页

作者： Javier Ortiz Juan carlos Paniagua Isidro cavaco Raquel Rivas Andrea Jimenez Luis chinchilla Elisa Muñoz María Dolores Ludeña departamento de biología celular y Patología Universidad de Salamanca (USAL) Salamanca Spain Servicio de Anatomía Patológica Hospital Universitario de Salamanca Salamanca Spain Servicio de Radiodiagnóstico Hospital Universitario de Salamanca Salamanca Spain

Objective: To describe the radiological, histological and immunohistochemical findings in a case of Progressive Multifocal Leukoencephalopathy (PML) affecting the cerebellar peduncles in a patient with chronic lymphocytic leukemia. Patient and Methods: Magnetic Resonance Imaging (MRI), histological picture (H.E., Kluver-Barrera) and immunohistochemical picture (GFAP, neurofilaments, cD68, Jc virus) were obtained. Results: 1) Magnetic resonance imaging: Asymmetric and progressive lesions on middle cerebellar peduncles, that were hyperintense in T2/FLAIR, extended towards the pons, had no mass effect and were unmodified after intravenous contrast. 2) Histology: Marked reactive gliosis with cytopathic changes suggesting viral infection, plus demyelination areas with axonal preservation. 3) Immunohistochemistry: Marked positivity for viral (polyoma and Jc virus) markers in glial cells showing cytopathic changes. conclusions: The importance of histological and immunohistochemical diagnosis in everyday assistance;of the collaboration between clinicians, radiologists and pathologists;and the validity of postmortem studies as a key element for research and clinical quality assessment must be stressed.

关键词： Progressive Multifocal Leukoencephalopathy Jc Virus Immunohistochemistry

Neuroprotection by salubrinal treatment in global cerebral ischemia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第11期11卷 1744-1745页

作者： Berta Anuncibay-Soto María Santos-Galdiano Arsenio Fernández-López Area de biologia celular Instituto de BiomedicinaUniversidad de León

Most common cerebrovascular accidents （cVAs） result from the occlusion of a blood vessel and are called ischemic stroke. The interruption of the blood flow reduces the oxygen and glucose levels in the neural pareuchyma, which in turn decreases the energy of the cells and compromises their homeostasis. This condition has to be reverted for cell survival. One of the homeostatic processes impaired by cerebral ischemia is protein folding in the endoplasmic reticulum （ER） lumen where unfolded proteins are accumulated （degracia and Monti, 2004）. This situation, known as ER stress, elicits the so called unfolded protein response （UPR）,

关键词： Neuroprotection by salubrinal treatment in global cerebral ischemia McAO BBB

clival Salivary Heterotopia (Salivary choristoma): A Rare Radiopathogical Lesión

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Open Journal of Pathology 2022年第4期12卷 115-119页

作者： Javier Ortiz Juan carlos Paniagua Luis chinchilla Enrique Montero Elisa Muñoz María Dolores Ludeña Dpto. de biología celular y Patología (Universidad de Salamanca) Servicio de Anatomía Patológica (Hospital Universitario de Salamanca) Salamanca Spain Servicio de Radiodiagnóstico (Hospital Universitario de Salamanca) Salamanca Spain

Objective: To describe the radiological and pathological presentation of a salivary heterotopia in clivus. The lesion was detected incidentally in a 57-year-old woman. Material and Methods: Radiologic imaging (cT scan and MRI) and histological picture obtained from a clival lesion. Results: Radiologically the lesion presented as a well-limited lytic lesion with a diameter of 1.5 cm and accompanied by a cerebrospinal fluid secondary fistula. Histological examination revealed non-pathological salivary gland tissue. conclusion: The differential diagnosis of this rare entity mandates a histological examination of the lesion.

关键词： Salivary Heterotopia clivus

Lipid-Polymer Membranes as carriers for L-Tryptophan: Molecular and Metabolic Properties

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Medicinal chemistry 2013年第1期3卷 31-39页

作者： Fernández Ruocco María Julieta Siri Macarena Igartúa Daniela Prieto María Jimena Alonso Silvia del Valle chiaramoni Nadia Silvia Instituto Multidisciplinario de biología celular Consejo Nacional de Investigaciones Científicas y Técnicas Buenos Aires Argentina Laboratory of Biomembranes Department of Science and Technology Universidad Nacional de Quilmes Buenos Aires Argentina

Polymerized liposomes encapsulating L-tryptophan were studied with the aim to characterize them as drug delivery systems for the treatment of several metabolic diseases that need an increased systemic L-tryptophan concentration. polymerized liposomes were obtained by UV irradiation of vesicles containing 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (Dc8,9Pc) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPc) in a 1:1 molar ratio, in the presence of 10 and 50 mol% of L-tryptophan (respect to total lipid concentration). Polymerization efficiency was studied spectrophotometrically. Also, bilayer packing at the polar head region was followed with the Merocyanine 540 (Mc540) and specific interactions in the lipopolymers were studied by FTIR. High L-tryptophan concentrations (50 mol% respect to total lipid concentration) induced a higher amount of six- and nine-unit polymers. This phenomenon was induced because the L-tryptophan located outside the lipid membrane was included in it during the polymerization process and was thus responsible for the better accommodate of the polar head region. This was not possible with the lower amount of L-tryptophan (10 mol%). The stability of lipopolymers with different amounts of L-tryptophan was studied through release profiles. Polymerized liposomes with 50 mol% of L-tryptophan were able to retain around 80% of the amino acid after 24 hours, whereas those with 10 mol % of the amino acid were able to retain 20%. The metabolic activity of the caco-2 cell line was also studied. cytotoxic effects were low in the presence of polymerized liposomes, rendering a maximum percentage of cell death of 30%. In summary, this work stresses the relevance of nonspecific drug-polymerized membrane binding on L-tryptophan pharmacological interaction with possible pharmaceutical applications in liposomal drug delivery. Moreover, the absence of significant cytotoxic effects allows the system proposed to be applied in human health.

关键词： Lipopolymers L-Tryptophan Drug delivery

Effects of living and metabolically inactive mesenchymal stromal cells and their derivatives on monocytes and macrophages

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem cells 2021年第9期13卷 1160-1176页

作者： Alexia Nedel Sant'Ana Anelise Bergmann Araújo Fabiany da costa Gonçalves Ana Helena Paz Laboratório de células Tecidos e Genes Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903RSBrazil centro de Processamento celular Hospital de Clínicas de Porto AlegrePorto Alegre 90035-903RSBrazil department of Internal Medicine Erasmus Medical CenterRotterdam 3015 GDNetherlands departamento de ciências Morfológicas Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto Alegre 90035-903RSBrazil

Mesenchymal stromal cells (MScs) are multipotent and self-renewing stem cellsthat have great potential as cell therapy for autoimmune and inflammatorydisorders, as well as for other clinical conditions, due to their immunoregulatoryand regenerative properties. MScs modulate the inflammatory milieu by releasingsoluble factors and acting through cell-to-cell mechanisms. MScs switch theclassical inflammatory status of monocytes and macrophages towards a nonclassicaland anti-inflammatory phenotype. This is characterized by an increasedsecretion of anti-inflammatory cytokines, a decreased release of pro-inflammatorycytokines, and changes in the expression of cell membrane molecules and inmetabolic pathways. The MSc modulation of monocyte and macrophage phenotypesseems to be critical for therapy effectiveness in several disease models, sincewhen these cells are depleted, no immunoregulatory effects are observed. Here,we review the effects of living MScs (metabolically active cells) and metabolicallyinactive MScs (dead cells that lost metabolic activity by induced inactivation) andtheir derivatives (extracellular vesicles, soluble factors, extracts, and microparticles)on the profile of macrophages and monocytes and the implications forimmunoregulatory and reparative processes. This review includes mechanisms ofaction exhibited in these different therapeutic appro-aches, which induce the antiinflammatoryproperties of monocytes and macrophages. Finally, we overviewseveral possibilities of therapeutic applications of these cells and their derivatives,with results regarding monocytes and macrophages in animal model studies andsome clinical trials.

关键词： Mesenchymal stromal cells Macrophage Monocyte Immunomodulation cell therapy Immunoregulation

<i>Trypanosoma evansi</i>: A Qualitative and Quantitative Ultrastructural Analysis of the Spleen during Experimental Murine Infections

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microscopy Research 2020年第3期8卷 31-42页

作者： Lucia Proietti d’Empaire Félix Tejero Hector J. Finol Pedro M. Aso Antonio Roschman-Gonzalez centro de biología Estructural Instituto Venezolano de Investigaciones Científicas Caracas Venezuela Instituto de Zoología y Ecología Tropical Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela centro de Microscopía Electrónica Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela departamento de biología celular Universidad Simón Bolívar Caracas Venezuela

A murine model is used to study qualitatively and quantitatively the splenic ultrastructural changes induced by two Trypanosoma evansi strains derived from naturally infected local equine hosts (Equusasinus and E. caballus);T. evansi causes ultrastructural modifications in the spleen of the infected mice. The modifications include tissular disorganization, fibrosis, mitochondrial swelling, apoptosis and necrosis. The initial phases of the infection are quite similar, whereas the final phases differ qualitatively depending on the strain’s source. The ultrastructural quantitative changes were studied in the reticular splenocytes covering alterations in the area of the cytoplasm and nucleus. Analysis of the results shows the induction of various splenic alterations caused by local T. evansi strains. Also, it was documented that discriminative time modulation, as well as progressive tissular, cellular and subcellular changes, are more associated with derived infections from E. caballus strain.

关键词： Trypanosoma evansi Spleen Ultrastructure Mouse

Functional mechanism study of the allelochemical myrigalone A identifies a group of ultrapotent inhibitors of ethylene biosynthesis in plants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant communications 2024年第6期5卷 246-262页

作者： George Heslop-Harrison Kazumi Nakabayashi Ana Espinosa-Ruiz Francesca Robertson Robert Baines christopher R.L.Thompson Katrin Hermann David Alabadí Gerhard Leubner-Metzger Robin S.B.Williams centre for Biomedical Sciences Department of Biological SciencesRoyal Holloway University of LondonEgham TW200EXUK centre for Plant Molecular Sciences Department of Biological SciencesRoyal Holloway University of LondonEgham TW200EXUK Instituto de biología Molecular y celular de Plantas(cSIc-UPV) 46022 ValenciaSpain centre for Life’s Origins and Evolution Department of GeneticsEvolution and EnvironmentUniversity College LondonLondonUK Syngenta Crop Protection AGSteinSwitzerland

Allelochemicals represent a class of natural products released by plants as root,leaf,and fruit exudates that interfere with the growth and survival of neighboring *** how allelochemicals function to regulate plant responses may provide valuable new approaches to better control plant *** such allelochemical,Myrigalone A(MyA)produced by Myrica gale,inhibits seed germination and seedling growth through an unknown ***,we investigate MyA using the tractable modelDictyostelium discoideum and reveal that its activity depends on the conserved homolog of the plant ethylenesynthesis protein 1-aminocyclopropane-1-carboxylic acid oxidase(AcO).Furthermore,in silico modeling predicts the direct binding of MyA to AcO within the catalytic *** ***,ablation of AcO mimics the MyA-dependent developmental delay,which is partially restored by exogenous ethylene,and MyA reduces ethylene *** Arabidopsis thaliana,MyA treatment delays seed germination,and this effect is rescued by exogenous *** also mimics the effect of established AcO inhibitors on root and hypocotyl extension,blocks ethylenedependent root hair production,and reduces ethylene ***,in silico binding analyses identify a rangeof highlypotentethylene inhibitorsthatblock ethylene-dependent responseand reduce ethyleneproduction in ***,we demonstrate a molecular mechanism by which the allelochemical MyA reduces ethylene biosynthesis and identify a range of ultrapotent inhibitors of ethylene-regulated responses.

关键词： 1-aminocyclopropane-1-carboxylic acid Acc Acc oxidase AcO allelochemicals ethylene synthesis inhibitors structure-activity relationship

Aryl hydrocarbon receptor as a new therapeutic target for cancer and immune disorders

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Pharmacology 2013年第4期2卷 107-114页

作者： Libia Vega Guillermo Elizondo departamento de Toxicología CINVESTAV-IPNZacatenco CP 07360 México city México departamento de biología celular CINVESTAV-IPNZacatenco CP 07360 México city México

The aryl hydrocarbon receptor （AhR） was discovered more than three decades ago, and initially was char-acterized as a transcription factor with a role in xe-nobiotic metabolism. However, based on subsequent observations that AhR remains active under physiologi-cal conditions, exhibits constitutive expression during development, and has a high degree of conservation among species, it was hypothesized that AhR is re-sponsible for functions in addition to its role in detoxif-cation. correspondingly, recent studies have elucidated novel physiological roles for this ligand-dependent transcription factor that link it to several pathways associated with disease development. In this review, studies are presented that support a role for AhR in cell proliferation, apoptosis, and immune homeostasis, thereby highlighting the therapeutic potential of this receptor for cancer and immune disorders.

关键词： Aryl hydrocarbon receptor cell proliferation Apoptosis Immunity cancer

Interplay between mesenchymal stromal cells and the immune system after transplantation: implications for advanced cell therapy in the retina

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第3期19卷 542-547页

作者： María Norte-Muñoz David García-Bernal Diego García-Ayuso Manuel Vidal-Sanz Marta Agudo-Barriuso Grupo de Investigación Oftalmología Experimental Departamento de OftalmologíaOptometríaOtorrinolaringología y Anatomía PatológicaFacultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la Salud30120 MurciaSpain Grupo de Investigación Trasplante Hematopoyético y Terapia celular Departamento de Bioquímica e Inmunología.Facultad de MedicinaUniversidad de MurciaInstituto Murciano de Investigación Biosanitaria(IMIB)Campus de Ciencias de la Salud30120 MurciaSpain

Advanced mesenchymal stromal cell-based therapies for neurodegenerative diseases are widely investigated in preclinical *** stromal cells are well positioned as therapeutics because they address the underlying mechanisms of neurodegeneration,namely trophic factor deprivation and *** studies have focused on the beneficial effects of mesenchymal stromal cell transplantation on neuronal survival or functional ***,little attention has been paid to the interaction between mesenchymal stromal cells and the host immune system due to the immunomodulatory properties of mesenchymal stromal cells and the long-held belief of the immunoprivileged status of the central nervous ***,we review the crosstalk between mesenchymal stromal cells and the immune system in general and in the context of the central nervous system,focusing on recent work in the retina and the importance of the type of transplantation.

关键词： adaptive immunity cell therapy central nervous system immune system innate immunity mesenchymal stromal cells neuroregeneration preclinical studies retina transplantation

Overexpression of the cholesterol-binding protein MLN64 induces liver damage in the mouse

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第22期13卷 3071-3079页

作者： Juan Enrique Tichauer María Gabriela Morales Ludwig Amigo Leopoldo Galdames Andrés Klein Verónica Quiones carla Ferrada Alejandra Alvarez R Marie-christine Rio Juan Francisco Miquel Attilio Rigotti Silvana Zanlungo departamento de Gastroenterología Facultad de Medicina Pontifi cia Universidad Católica Santiago Chile departamento de biología celular y Molecular Facultad de Ciencias Biológicas Pontificia Universidad Católica Santiago Chile Institut de Génétique et de biologie Moléculaire et cellulaire INSERM U596 67404 Illkirch France

AIM： To examine the in vivo phenotype associated with hepatic metastatic lymph node 64 （MLN64） overexpression.METHODS： Recombinant-adenovirus-mediated MLN64 gene transfer was used to overexpress MLN64 in the livers of c57BL/6 mice. We measured the effects of MLN64 overexpression on hepatic cholesterol content, bile flow, biliary lipid secretion and apoptosis markers. For in vitro studies cultured cHO cells with transient MLN64 overexpression were utilized and apoptosis by TUNEL assay was measured.RESULTS： Livers from ***64-infected mice exhibited early onset of liver damage and apoptosis. This response correlated with increases in liver cholesterol content and bilian/bile acid concentration, and impaired bile flow. We investigated whether liver MLN64 expression could be modulated in a murine model of hepatic injury. We found increased hepatic MLN64 mRNA and protein levels in mice with chenodeoxycholic acid-induced liver damage. In addition, cultured cliO cells with transient MLN64 overexpression showed increased apoptosis.cONcLUSION： In summary, hepatic MLN64 overexpression induced damage and apoptosis in murine livers and altered cholesterol metabolism. Further studies are required to elucidate the relevance of these findings under physiologic and disease conditions.

关键词： Metastatic lymph node 64 Apoptosis cholesterol Liver StarD3