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chemical profile and antimicrobial activity of Secondatia floribunda A. Dc(Apocynaceae)

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 739-749页

作者： Daiany Alves Ribeiro Sarah Soares Damasceno Aline Augusti Boligon Irwin Rose Alencar de Menezes Marta Maria de Almeida Souza JoséGalberto Martins da costa Programa de Pós-Graduacao em Etnobiologia e conservacao da Natureza Universidade Regional do Cariri Departamento de química biológica Laboratório de Pesquisas de Produtos NaturaisUniversidade Regional do Cariri Laboratório de Fitoquímica Universidade Federal de Santa Maria Departamento de química biológica Laboratório de Farmacologia e Química MolecularUniversidade Regional do Cariri Departamento de ciências biológicas Laboratório de Ecologia VegetalUniversidade Regional do Cariri

To establish the chemical profile, and to evaluate the antibacterial and modulatory activity of the ethanolic extracts of the stalk’s inner bark and heartwood of Secondatia *** of total phenols and flavonoids was determined by the Folin-ciocalteu method and aluminum chloride, respectively. Phenolic compounds were identified and quantified by HPLc-DAD (High Performance Liquid chromatography-Diodearray Detector) and the Infrared Spectroscopy was performed using the measure by Attenuated Total Reflectance with Fourier Transform (ATR-FTIR). Antibacterial assays for determination of the Minimum Inhibitory concentration (MIc) and modification of aminoglycosides were performed by *** spectra showed similar characteristics, having among its main absorption bands hydroxyl group (OH). The antibacterial activity showed clinically significant results for the strains of Staphylococcus aureus and Escherichia coli. In modulation assay, synergic and antagonistic effect for both extracts was observed. Heartwood extract in combination with antibiotics showed a significant MIc reduction at 19.8% (P conclusionsThis study is the first report of chemical and biological information of Secondatia floribunda suggesting that it is clinically relevant source of a new antibacterial therapy, especially due to the presence of significant levels of phenolic compounds.

关键词： chemical composition HPLc cinchonain Natural product Modification resistance

cRISPR/cas9 editing of the polygalacturonase FaPG1 gene improves strawberry fruit firmness

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Horticulture Research 2023年第3期10卷 201-210页

作者： Gloria López-casado cristina Sánchez-Raya Pablo D.Ric-Varas candelas Paniagua Rosario Blanco-Portales Juan Muñoz-Blanco Sara Pose Antonio J.Matas Jose A.Mercado Departamento de Botánica y Fisiología vegetal Instituto de Hortofruticultura Subtropical y Mediterránea‘La Mayora’(IHSM-UMA-CSIC)Universidad de Málaga29071 MálagaSpain Departamento de Bioquímica y biología Molecular Universidad de Córdoba14071CórdobaSpain

Firmness is one of the most important fruit quality traits in *** postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important *** studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry *** this study,FaPG1 knockout strawberry plants have been generated using the cRISPR/cas9 system delivered via Agrobacterium *** independent lines,cv.“chandler”,were obtained,and all of them were successfully edited as determined by PcR amplification and T7 endonuclease *** targeted mutagenesis insertion and deletion rates were analyzed using targeted deep *** percentage of edited sequences varied from 47%up to almost 100%,being higher than 95%for seven of the selected *** analyses showed that 7 out of the eight lines analyzed produced fruits significantly firmer than the control,ranging from 33 to 70%increase in *** was a positive relationship between the degree of FaPG1 editing and the rise in fruit *** changes were observed in other fruit quality traits,such as colour,soluble solids,titratable acidity or anthocyanin *** fruits showed a reduced softening rate during postharvest,displayed a reduced transpirational water loss,and were less damaged by Botrytis cinerea *** analysis of four potential off-target sites revealed no mutation *** conclusion,editing the FaPG1 gene using the cRISPR/cas9 system is an efficient method for improving strawberry fruit firmness and shelf life.

关键词： fir straw PG1

In Vitro Propagation of Agave guiengola Gentry Using Semisolid Medium and Temporary Immersion Bioreactors

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 1003-1013页

作者： Lucía Isabel chávez-Ortiz JoséFrancisco Morales-Domínguez Araceli Rodríguez-Sahagún Eugenio Pérez-Molphe-Balch Departamento de química Centro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientes20131México Laboratorio de biología Molecular vegetal Centro Universitario de la CiénegaUniversidad de GuadalajaraOcotlán47820México

Agave guiengola Gentry is an endemic plant from a very small locality in Oaxaca,*** conservation status is fragile and can rapidly *** of its scarcity,this agave has been used solely for ornamental purposes,but it could have other uses if more plants were *** vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium(MS)as well as basal medium supplemented with cytokinins 6-Benzylaminopurine(BA)or 6-(γ,γ-Dimethylallylamino)purine(2iP).The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^(–1) BA,obtaining a mean of 3.7 shoots per *** interesting responses were observed,such as nodular callus induction using combinations of BA and 2,4-Dichlorophenoxyacetic acid(2,4-D);root induction without Plant Growth Regulators(PGR);and generation of shoot *** clusters constituted an excellent explant for micropropagation in temporary immersion bioreactors,obtaining a propagation rate of 43 shoots per explant with 1 min immersion and 6 h immersion *** new plants rooted and survived the transfer to *** study developed an in vitro propagation scheme to produce individuals that can be used either for reforestation,economical purposes,or to carry out studies in this species to assess its full potential,avoiding exploitation from wild plants.

关键词： Agavaceae temporary immersion systems callus induction micropropagation

Selective logging alters allometric relationships of five tropical tree species in seasonal semi-deciduous forests

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Journal of Forestry Research 2019年第5期30卷 1633-1639页

作者： Diego Resende Rodrigues Yves Rafael Bovolenta José Antonio Pimenta Edmilson Bianchini Departamento de biologia Animal e vegetal Centro de Ciências Biológicas Universidade Estadual de Londrina

In selectively logged forests,trees are more likely to expand their diameters(D)at the expense of height(H)growth,resulting in variations in H:D *** study examines how selective logging affects the H:D allometric relationships of five common tree species and whether the effects vary with functional groups(shade-intolerant or shade tolerant)in seasonal semi-deciduous *** of five species in a 3000 m^2(0.3 ha)plot were marked and heights and diameters *** of the species,with one exception,showed greater investment in diameter per increment of height compared to an unlogged forest,possibly because of the greater light *** study shows the effects of selective logging on species populations as evidenced by increases in H:D *** of forest fragments with different degrees of human impact is important because it allows us to understand the differences in architectural characteristics caused by selective logging.

关键词： Allometry Anthropic exploitation Ecological groups Luminosity Selective logging

cysteine and cysteine-Related SignalingPathways in Arabidopsis thaliana

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Molecular Plant 2014年第2期7卷 264-276页

作者： Luis c. Romero M. Ángeles Aroca Ana M. Laureano-Marín Inmaculada Moreno Irene García cecilia Gotor Instituto de Bioquímica vegetal y Fotosíntesis Consejo Superior de Investigaciones Científicas and Universidad de Sevilla Avenida Américo Vespucio 49 41092 Sevilla Spain

cysteine occupies a central position in plant metabolism because it is a reduced sulfur donor moleculeinvolved in the synthesis of essential biomolecules and defense compounds. Moreover, cysteine per se and its deriva-tive molecules play roles in the redox signaling of processes occurring in various cellular compartments. cysteine issynthesized during the sulfate assimilation pathway via the incorporation of sulfide to O-acetylserine, catalyzed byO-acetylserine（thiol）lyase （OASTL）. Plant cells contain OASTLs in the mitochondria, chloroplasts, and cytosol, resultingin a complex array of isoforms and subcellular cysteine pools, in recent years, significant progress has been made inArabidopsis, in determining the specific roles of the OASTLs and the metabolites produced by them. Thus, the dis-covery of novel enzymatic activities of the less-abundant, like DES1 with L-cysteine desulfhydrase activity and ScSwith S-sulfocysteine synthase activity, has provided new perspectives on their roles, besides their metabolic ***, the research has been demonstrated that cytosolic sulfide and chloroplastic S-sulfocysteine act as signalingmolecules regulating autophagy and protecting the photosystems, respectively. In the cytosol, cysteine plays an essentialrole in plant immunity; in the mitochondria, this molecule plays a central role in the detoxification of cyanide, which isessential for root hair development and plant responses to pathogens.

关键词： β-cyanoalanine synthase cysteine desulfhydrase cyanide O-acetylserine(thiol)lyase plant development plant immunity redox signaling S-sulfocysteine synthase stress sulfide

Variable retention harvesting: conceptual analysis according to different environmental ethics and forest valuation

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Ecological Processes 2019年第1期8卷 485-491页

作者： Leonardo Galetto carolina Torres Guillermo J.Martínez Pastur Departamento de Diversidad biológica y Ecología FCEFyNUniversidad Nacional de Córdoba(UNC)Vélez Sarsfield 16115000 CórdobaArgentina Instituto Multidisciplinario de biología vegetal(IMBIV) Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Vélez Sarsfield 16115000 CórdobaArgentina centro Austral de Investigaciones científicas(cADIc) Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Houssay 2009410 UshuaiaTierra del FuegoArgentina.

Background:conceptual clarity is important to attain precise communication of scientific knowledge and to implement appropriate technological and policy *** concepts referring to forest management are widely used by decision-makers,regardless of their *** the scientific and methodological issues of forestry practices are frequently discussed in the literature,their normative dimensions are rarely ***,linguistic uncertainty increases when different environmentally ethical perspectives and ways of valuing forests are *** objective was to compare different conceptualizations on the silvicultural systems suggested for forest management and the implications they have for *** have conceptually contrasted highintensity forestry practices with variable retention harvesting,considering different environmentally ethical perspectives and forest valuation ***:clear boundaries between clear-cutting,selective logging,and variable retention harvesting can be evidenced when different ethical points of view and alternatives in the human-nature relationships are *** have found a variety of definitions of variable retention harvesting that can be analyzed under different ethical *** contrasting views on variable retention harvesting can be evidenced if nature is considered to be purely at human’s service or if it is conceptualized as humans co-inhabiting with *** latter position implies that the maintenance of ecological,evolutionary,and historical processes supported by unmanaged forest stands is a crucial step for forest management proposals based on variable retention ***:Forestry practices that are focused on forest yields and that misinterpret functional uncertainty of forest functioning would be ***,forestry with variable retention harvesting could imply good yields with reasonable conservation management in some contexts,while it could be unacce

关键词： Environmental ethical perspectives consequentialism Deontological ethics Virtue ethics Policy options for forest and biocultural conservation

THERMAL PERFORMANcE OF AN EXTENSIVE GREEN ROOF UNDER SEMI-ARID cONDITIONS IN cENTRAL ARGENTINA

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Journal of Green Building 2021年第1期16卷 17-42页

作者： Lelia Imhof Edgardo Suárez Natalia cáceres Federico Robbiati cecilia cáceres Alejandra Broilo Lucila Pellizari Mario Suárez Emmanuel Hick Evangelina Matoff Leonardo Galetto IRNASUS(Facultad de ciencias Agropecuarias-Facultad de Arquitectura Universidad Católica de Córdoba-CONICET Instituto de Etiquetación Edilicia Urbana Colegio de Arquitectos de la Provincia de Córdoba Departamento de Diversidad biológica y Ecología Facultad de Ciencias ExactasFísicas y NaturalesUniversidad Nacional de Córdoba e Instituto Multidisciplinario de Biología Vegetal(CONICET-UNC) AER INTA Agencia de Extensión Rural Presidente Roca esquina La CoruñaCórdoba

Extensive green roofs improve the provision of ecosystem services in urban environ-ments,particularly in semiarid *** aim of this paper is to compare their thermal performance during six months between two rooms,one with a green roof and the other with a conventional roof,in córdoba(Argentina).The room with a green(planting)roof showed a lower inside surface temperature since the begin-ning of the study than the control room(between 5-6°c of difference).During the selected period,the indicators such as temperature amplitude(the difference between the maximum and the average temperature)and the anti-interference characteristics of the layers to the outdoor air temperature are produced a better performance for the green roof compared to the conventional *** pattern of a better perfor-mance was consistent across the study for the green roof,characterized by a higher cooling and warming of the roof surface during the day and night,*** green roof was more effective at blocking an upward heat flux during the day and suppressing heat loss during the ***,conductive flux and climatic conditions seem to dominate the thermal performance of green roofs in areas with semiarid climate conditions.

关键词： thermal performance extensive green roofs semiarid region

Sarcoma stratification by combined pH2AX and MAP17(PDZK1IP1)levels for a better outcome on doxorubicin plus olaparib treatment

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Signal Transduction and Targeted Therapy 2020年第1期5卷 697-709页

作者： Marco Perez JoséManuel García-Heredia Blanca Felipe-Abrio Sandra Muñoz-Galván Javier Martín-Broto Amancio carnero Instituto de Biomedicina de Sevilla(IBIS) Hospital Universitario Virgen del RocíoUniversidad de SevillaConsejo Superior de Investigaciones CientíficasSevillaSpain ciber de cancer IS Carlos IIIMadridSpain Departamento de Bioquímica vegetal y biología Molecular Universidad de SevillaSevillaSpain Departamento de Anatomía Patológica Hospital Universitario Virgen del RocíoSevillaSpain

Sarcomas constitute a rare heterogeneous group of tumors,including a wide variety of histological *** advances in our understanding of the pathophysiology of the disease,first-line sarcoma treatment options are still limited and new treatment approaches are *** H2AX phosphorylation is a sensitive marker for double strand breaks and has recently emerged as biomarker of DNA damage for new drug *** this study,we explored the role of H2AX phosphorylation at Ser139 alone or in combination with MAP17 protein,an inducer of DNA damage through ROS increase,as prognostic biomarkers in sarcoma ***,we proposed doxorubicin and olaparib combination as potential therapeutic strategies against sarcomas displaying high level of both *** evaluate retrospectively the levels of pH2AX(Ser139)and MAP17 in a cohort of 69 patients with different sarcoma types and its relationship with clinical and pathological *** found that the levels of pH2AX and MAP17 were related to clinical features and poor ***,we pursued PARP1 inhibition with olaparib to potentiate the antitumor effect of DNA damaging effect of the DNA damaging agent doxorubicin to achieve an optimal synergy in *** demonstrated that the combination of olaparib and doxorubicin was synergistic in vitro,inhibiting cell proliferation and enhancing pH2AX intranuclear accumulation,as a result of DNA *** synergism was corroborated in patient-derived xenografts(PDX)where the combination was effective in tumors with high levels of pH2AX and MAP17,suggesting that both biomarkers might potentially identify patients who better benefit from this combined therapy.

关键词： H2AX doxorubicin treatment

Identification of Rhizobia Isolated from Nodules of Mexican commercial Soybean Varieties

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American Journal of Plant Sciences 2024年第1期15卷 29-45页

作者： cecilia Vázquez Rodríguez Lourdes Vital López Jesús Gerardo García Olivares Homar Rene Gill Langarica Laboratorio de Biotecnología vegetal Centro de Biotecnología Genómica Instituto Politécnico Nacional Reynosa México carrera de Mantenimiento Industrial Universidad Tecnológica de Tamaulipas Norte Universidad Tecnológica Reynosa México

Rhizobia, crucial for nitrogen fixation in leguminous plants, play a vital role in soybean cultivation. This study, conducted in Mexico, a major soybean importer, aimed to identify bacteria from nodules of five soybean varieties in high-production regions. Multilocus sequence analysis (MLSA) was employed for enhanced species resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA 76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icense. Bradyrhizobium japonicum USDA 110 predominated in the soils, displaying symbiotic preference for the Huasteca 400 variety. However, phylogenetic analysis didn't reveal a clear association between strains, soil, and soybean variety. This research sheds light on the diversity of rhizobia in Mexican soybean cultivation, contributing to the understanding of symbiotic relationships in soybean production systems.

关键词： Nodules Soybean Housekeeping Genes MLSA Rhizobia Bradyrhizobium Nitrogen Fixation Symbiosis Phylogenetic Analysis