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Expression And Clinical Significance of Glucose Regulated Proteins GRP78 And GRP94 in Human Colon Cancer

Chinese Journal of Cancer Research 2010年第1期22卷 42-48页

作者： Ming-hua Liu Meng-chunWang Na Gao Yan Li Wei-guo Jiang Department of Gastroenterology Department of pathology Shengjing Hospital China Medical University Shenyang 110004 China Endoscopy center Liaoning Tumour Hospital Shenyang 110000 China

Objective： To investigate the expression of glucose regulated proteins GRP78 and GRP94 in human colon cancer. Methods： Tissues of resected primary colon cancer, colon adenoma and normal tissue were investigated. Protein expression was detected with immunohistochemical staining, mRNA expression levels of GRP78 and GRP94 were determined by semiquantitative reverse transcriptase polymerase chain reaction （RT-PCR） after mRNA extraction. Results： The expression of GRP94 and GRP78 was significantly higher in colon cancer when compared to those in colon adenoma and normal tissue （P〈0.01）. GRP94 mRNA and protein expression was found to be in close relationship with the grade of differentiation, Dukes stages, lymph node involvement and remote metastasis in colon cancer （P〈0.01）, but no relationship with gender and age （P〉0.05）. GRP78 mRNA and protein expression increased with cancer progression along the normal tissue-adenoma-cancer sequence, but showed no association with grade of differentiation, Dukes stages, lymph node involvement, remote metastasis, gender and age （P〉0.05）. The mRNA expression of GRP78 and GRP94 was consistent with the proteins （P〈0.01）, but there is no correlation between overexpression of GRP78 and GRP94 （P〉0.05）, and the patients with both strong GRP78 and GRP94 protein expression did not show advanced tumor stages （P〉0.05）. Conclusion： Overexpression of GRP78 and GRP94 was found in colon cancer. Overexpression of GRP94 was closely related to cellular differentiation, Dukes stages, invasion and metastasis.

关键词： Colon cancer GRP78 GRP94 Colon adenoma Dukes stages Metastasis

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Polymorphism of CYPIAl and GSTMl genes associated with susceptibility of gastric cancer in Shandong Province of China

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World Journal of Gastroenterology 2005年第37期11卷 5757-5762页

作者： Hao Li Xue-Liang Chen Hui-Qing Li the center of tumour Treatment Qilu Hospital of Shandong University Jinan 250012 Shandong Province China the Institute of Basic Medicine Shandong Academy of Medical Sciences Jinan 250062 Shandong Province China

AIM： To explore whether polymorphisms of the CYPIA1 and GSTM1 genes are associated with susceptibility of stomach cancer. METHODS： A total of 102 stomach cancer cases and 62 healthy persons were diagnosed by pathology in 1998- 2000 in the Qilu Hospital of Shandong University. Gene polymorphisms were detected by the PCR using sequencespecific primers. Data analysis of the case-control study was carried out using the unconditional logistic method. RESULTS： After adjustment for age, sex, educational levels, and occupation, the risk factors for stomach cancer were shown to be smoking, Helicobacter pylori （H pylon）, and presence of the CYPIM G/G and GSTM1O/O genotypes. Interaction was observed between the combined genotypes of either CYPIA1 G/G and GSTM1O/O or Hpyloriinfection, or GSTM1O/O and Hpyloriinfection or smoking. CONCLUSION： Polymorphisms of the CYPIA1 and GSTM1 genes, Hpyloriinfection and smoking are related to susceptibility to stomach cancer.

关键词： Stomach cancer CYPIA1 GSTM1 Polymorphism

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Microglia Suppresses Breast Cancer brain Metastasis via a Pro-inflammatory Response

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Neuroscience Bulletin 2024年第7期40卷 1034-1036页

作者： Shengbo Chen Yi-Jun Liu Department of Neurobiology of Affiliated Mental Health center and Hangzhou Seventh People's Hospital and School of brain Science and brain Medicine Zhejiang University School of MedicineHangzhou 310058China Liangzhu Laboratory MOE Frontier Science Center for Brain Science and Brain-machine IntegrationState Key Laboratory of Brain-machine IntelligenceZhejiang University1369 West Wenyi RoadHangzhou 311121China NHC and CAMS Key Laboratory of Medical Neurobiology Zhejiang UniversityHangzhou 310058China

Due to the obstacle of the blood-brain barrier(BBB)for drug delivery[1],breast cancer brain metastasis(BCBM)represents a worldwide health challenge with high *** BCBM formation and progression,brain metastases(BrMs)secret various factors,including cytokines and exosomes,and trigger the infiltration of tumours-associated macrophages(TAMs),which in turn become important components of brain tumour microenvironments(TME).

关键词： mortality tumour Breast

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Loss of Wip1 aggravates brain injury after ischaemia/reperfusion by overactivating microglia

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Stroke & Vascular Neurology 2021年第3期6卷 344-351页

作者： Feng Yan Xiang Cheng Ming Zhao Shenghui Gong Ying Han Liping Ding Di Wu Yumin Luo Wei Zuo Lingling Zhu Ming Fan Xunming Ji Beijing Institute of brain Disorders Laboratory of Brain DisordersMinistry of Science and TechnologyCollaborative Innovation Center for Brain DisordersCapital Medical UniversityBeijingChina Cerebrovascular Disease Research Laboratory Xuanwu HospitalBeijingChina Institute of Military Cognition and brain Sciences Academy of Military Medical SciencesBeijingChina Department of Pharmacy Peking Union Medical College HospitalDongcheng-quBeijingChina Co-Innovation center of Neuroregeneration Nantong UniversityNantongChina

Background and purpose The inflammatory response mediated by microglia/macrophages is closely related to cerebral ischaemia/reperfusion ***-type p53-induced protein phosphatase 1(Wip1),a serine/threonine phosphatase,is expressed in various tissues.A growing number of reports have suggested that Wip1 is a negative regulator of inflammation in peripheral tissue;however,its role in the central nervous system(CNS)remains *** study aimed to clarify whether Wip1 can inhibit CNS inflammation by regulating microglia/macrophage functions after ischaemic *** A model of middle cerebral artery occlusion and reperfusion was established in *** inflammation was simulated by lipopolysaccharide treatment of primary *** speckle imaging was used to monitor regional cerebral blood *** outcomes were assessed with a TreadScan gait analysis *** staining was used to evaluate the infarct volume,and western blotting and immunofluorescence staining were applied to detect the phenotypical transformation of *** was performed to detect the levels of inflammatory *** Wip1 expression was increased after ischaemia/***1-knockout(KO)mice displayed more severe brain injury than wild-type mice,as indicated by aggravated motor dysfunction,greater brain infarct volumes and higher expression of inflammatory cytokines(interleukin-6 and tumour necrosis factor alpha)in the *** also found that Wip1 depletion increased microglial/macrophage activation in both in vitro and in vivo models,which all showed activation of microglia/***-Ppm1d reversed the injury induced by *** Our results suggest that Wip1 may inhibit neuroinflammation by inhibiting microglial/macrophage activation after brain ischaemia/reperfusion injury.

关键词： inflammation reperfusion Wip1

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LIN9 confers paclitaxel resistance in triple negative breast cancer cells by upregulating CCSAP

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Science China(Life Sciences) 2020年第3期63卷 419-428页

作者： Hongna Lai Rui Wang Shunying Li Qianfeng Shi Zijie Cai Yudong Li Yujie Liu Guangdong Provincial Key Laboratory of Malignant tumour Epigenetics and Gene Regulation Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Breast tumour center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Division of Vascular Surgery First Affiliated HospitalSun Yat-Sen UniversityGuangzhou 510080China

LIN9 functions to regulate cell mitotic *** of LIN9 expression is associated with development of human *** this study we assessed the association of LIN9 expression with paclitaxel resistance and clarified the underlying mechanisms for the first ***9 expression in breast cancer tissues was retrieved from publicly available online databases and statistically *** TNBC cell lines MDA-MB-231 and MDA-MB-468 and their corresponding paclitaxelresistant sublines 231PTX and 468PTX were used to assess the expression of LIN9 by qRT-PCR and Western blot,cell growth by cell counting,cell viability by MTS assay,and cell apoptosis by flow *** data showed that high LIN9 expression in breast cancer patients receiving chemotherapy was related to poor overall survival(OS).LIN9 expression was upregulated in paclitaxel-resistant TNBC cells compared to their parental *** of LIN9 or treatment of paclitaxel-resistant TNBC cells with a bromo-and extra-terminal domain inhibitor(BETi)JQ1 which also decreased LIN9 expression enhanced the sensitivity of paclitaxel-resistant TNBC cells to ***,decreased LIN9 in resistant cell lines reduced tumor cell viability,promoted multinucleated cells formation and induced tumor cell apoptosis,potentially by directly regulating microtubule-binding protein *** conclusion,high LIN9 expression contributed to poor clinical outcomes and paclitaxel resistance in TNBC and BETi,targeting LIN9 expression,could be a reversible drug for PTX-resistant TNBC patients.

关键词： LIN9 triple negative breast cancer paclitaxel resistance BET inhibitor

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Review:Development of SARS-CoV-2 immuno-enhanced COVID-19 vaccines with nano-platform

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Nano Research 2022年第3期15卷 2196-2225页

作者： Nawamin Sa-nguanmoo Katawut Namdee Mattaka Khongkow Uracha Ruktanonchai YongXiang Zhao Xing-Jie Liang CAS center for Excellence in Nanoscience CAS Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyNational Center for Nanoscience and Technology of ChinaBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China National Nanotechnology center(NANOTEC) National Science and Technology Development AgencyPathum Thani 12120Thailand National center for International Research of Biotargeting Theranostics Guangxi Key Laboratory of Biotargeting TheranosticsCollaborative Innovation Center for Targeting Tumour Theranostics and TherapyGuangxi Medical UniversityNanning 530021China

Vaccination is the most effective way to prevent coronavirus disease 2019(COVID-19).Vaccine development approaches consist of viral vector vaccines,DNA vaccine,RNA vaccine,live attenuated virus,and recombinant proteins,which elicit a specific immune *** use of nanoparticles displaying antigen is one of the alternative approaches to conventional *** is due to the fact that nano-based vaccines are stable,able to target,form images,and offer an opportunity to enhance the immune *** diameters of ultrafine nanoparticles are in the range of 1–100 *** application of nanotechnology on vaccine design provides precise fabrication of nanomaterials with desirable properties and ability to eliminate undesirable *** be successful,nanomaterials must be uptaken into the cell,especially into the target and able to modulate cellular functions at the subcellular *** advantages of nano-based vaccines are the ability to protect a cargo such as RNA,DNA,protein,or synthesis substance and have enhanced stability in a broad range of pH,ambient temperatures,and humidity for long-term ***,nano-based vaccines can be engineered to overcome biological barriers such as nonspecific distribution in order to elicit functions in antigen presenting *** this review,we will summarize on the developing COVID-19 vaccine strategies and how the nanotechnology can enhance antigen presentation and strong immunogenicity using advanced technology in nanocarrier to deliver *** discussion about their safe,effective,and affordable vaccines to immunize against COVID-19 will be highlighted.

关键词： COVID-19 SARS-CoV-2 vaccine nanotechnology antigen presenting cells(APCs)

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Characteristics of homologous recombination repair pathway genes mutation in ovarian cancers

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Cancer Innovation 2022年第3期1卷 220-228页

作者： Zongbi Yi Min Chen Shaoxing Sun Chunxu Yang Zijie Mei Hui Yang Qingming Xiang Hui Qiu Department of Radiation and Medical Oncology Hubei Key Laboratory of Tumour Biological BehaviorsHubei Cancer Clinical Study CenterZhongnan Hospital of Wuhan UniversityWuhanChina

Background:Few studies have investigated the characteristics of non‐BRCA homologous recombination repair(HRR)pathway somatic mutations,and the impact of these mutations on efficacy of treatment in ovarian cancer patients is not ***,we conducted this study to analyze the frequency and spectrum of somatic mutations in HRR pathway genes in patients with ovarian cancer and to examine the relationships between somatic mutations in HRR pathway genes and their effects on the efficacy of platinum‐based ***:We performed targeted sequencing of 688 genes related to the occurrence,development,treatment,and prognosis of solid *** mutations were identified by paired analysis of tumor tissue and germline DNA in blood ***:A total of 38 patients with ovarian cancer were included in the study,and 35(92.1%)patients were diagnosed with high‐grade serous *** patients exhibited somatic mutations in the tumor tissue *** commonly mutated genes were TP53(73.7%),BRCA2(55.3%),NF1(52.6%),BRCA1(47.4%),and CDH1(47.4%).Overall,71.1%of the patients exhibited mutation in at least one HRR pathway *** most frequently altered HRR genes were BRCA2(55.3%),followed by BRCA1(47.4%),ATM(44.7%),BARD1(42.1%),and CHEK1(36.8%).The median progression‐free survival(PFS)in patients with HRR pathway mutation was 36.0 months compared with 13.6 months in patients with no HRR pathway mutation(hazard ratio[HR],0.25;95%confidence interval[CI],0.08–0.77;p=0.016).Patients harboring BRCA1/2 and/or CDK12 mutations displayed a longer PFS(median,36.0 months)compared with patients with no BRCA1/2 or CDK12 mutation(median,13.6 months;HR,0.21;95%CI,0.07–0.61;p=0.004).In multivariate analysis Cox proportional hazards models,after adjustment for tumor stage at diagnosis and histology of initial diagnosis,patients with HRR pathway mutation had a longer PFS than patients with HRR wild‐type genes(p=0.006).Conclusions:HRR pathway somatic mutations are common in

关键词： ovarian cancer genetic testing somatic mutation homologous recombination repair platinum-based therapy

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Mammary stem cells:angels or demons in mammary gland?

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Signal Transduction and Targeted Therapy 2017年第1期2卷 312-319页

作者： Xueman Chen Qiang Liu Erwei Song Breast Tumor center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Malignant tumour Epigenetics and Gene Regulation Medical Research CenterSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhouChina

A highly dynamic development process exits within the epithelia of mammary gland,featuring morphogenetic variation during puberty,pregnancy,lactation,and *** identification of mammary stem cells(MaSCs)via lineage-tracing studies has substantiated a hierarchical organization of the mammary epithelia.A single MaSC is capable of reconstituting the entirely functional mammary gland upon orthotopic *** different mammary cell subpopulations can be candidate cells-of-origin for distinct breast tumor subtypes,it still lacks experimental proofs whether MaSCs,the most primitive cells,are the‘seeds’of malignant transformation during most,if not all,tumorigenesis in the ***,we review current knowledge of mammary epithelial hierarchy,highlighting the roles of mammary stem/progenitor cells and breast cancer stem cells(BCSCs)along with their key molecular regulators in organ development and cancer *** these issues will pave the way for developing novel interventions toward stem/progenitor cells in either prevention or treatment of breast cancer(BrCa).

关键词： prevention breast entirely

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RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway

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Frontiers of Medicine 2023年第1期17卷 143-155页

作者： Chaochao Wang Hao Xue Rongrong Zhao Zhongzheng Sun Xiao Gao Yanhua Qi Huizhi Wang Jianye Xu Lin Deng Gang Li Department of Neurosurgery Qilu HospitalCheeloo College of Medicine and Institute of Brain and Brain-Inspired ScienceShandong UniversityJinan 250012China Shandong Key Laboratory of brain Function Remodeling Jinan 250012China The Second Hospital Cheeloo College of MedicineShandong UniversityJinan 250033China

Gliomas are the most common central nervous system tumours;they are highly aggressive and have a poor prognosis. RGS16 belongs to the regulator of G-protein signalling (RGS) protein family, which plays an important role in promoting various cancers, such as breast cancer, pancreatic cancer, and colorectal cancer. Moreover, previous studies confirmed that let-7c-5p, a well-known microRNA, can act as a tumour suppressor to regulate the progression of various tumours by inhibiting the expression of its target genes. However, whether RGS16 can promote the progression of glioma and whether it is regulated by miR let-7c-5p are still unknown. Here, we confirmed that RGS16 is upregulated in glioma tissues and that high expression of RGS16 is associated with poor survival. Ectopic deletion of RGS16 significantly suppressed glioma cell proliferation and migration both in vitro and in vivo. Moreover, RGS16 was validated as a direct target gene of miR let-7c-5p. The overexpression of miR let-7c-5p obviously downregulated the expression of RGS16, and knocking down miR let-7c-5p had the opposite effect. Thus, we suggest that the suppression of RGS16 by miR let-7c-5p can promote glioma progression and may serve as a potential prognostic biomarker and therapeutic target in glioma.

关键词： RGS16 let-7c-5p glioma proliferation migration

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Bamboo‑Inspired Gasotransmitter Microfibres for Wound Healing

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Advanced Fiber Materials 2023年第1期5卷 388-399页

作者： Cheng Zhao Jiahui Guo Han Zhang Min Nie Caihong Yang Yujuan Zhu Yuanjin Zhao Liping Zhong Department of Rheumatology and Immunology Institute of Translational MedicineThe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210002China National center for International Biotargeting Theranostics Guangxi Key Laboratory of Biotargeting TheranosticsCollaborative Innovation Center for Targeting Tumour TheranosticsGuangxi Medical UniversityGuangxi 530021China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Diabetic wounds have become a major clinical problem that cannot be ***,such as hydrogen sulphide(H_(2)S),have demonstrated value in inducing angiogenesis and accelerating wound healing,while their effective delivery is still ***,inspired by the continuous-independent hollow structure of bamboo,we propose novel gasotransmitter microfibres with septal H_(2)S bubbles using microfluidic spinning for diabetic wound *** from the exact control of microfluidics,gasotransmitter microfibres with different bubble sizes and morphologies could be generated successfully and *** the dual effects of drugs in the shell and gas in the core,the wound healing process could be ***,the controllable release of drugs could be achieved by adding responsive materials into the microfiber shell,which would promote continuous effects of contents on *** on in vitro and in vivo stud-ies,we have proven that these gasotransmitter microfibres have a positive impact on inducing angiogenesis and promoting cell proliferation during wound ***,it is believed that the bamboo-inspired gasotransmitter microfibres will have important value in gasotransmitter research and clinical applications.

关键词： Microfluidics Hydrogel Wound healing Bioinspired Gasotransmitter

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