检索结果-南通市图书馆

Synthesis, characterization and in vivo evaluation of honokiol bisphosphate prodrugs protects against rats’ brain ischemia-reperfusion injury

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Pharmaceutical Sciences 2019年第6期14卷 640-648页

作者： Gaojie Xu Renghan Dong Jin Liu Li Zhao Yan Zeng Xiaofan Xiao Jinglin An Sheng Huang Yueling Zhong Bing Guang Tai Yang Chengdu Medical co.lege Chengdu 610500China Chengdu Yishan biotechnology co. Ltd.Chengdu 610094China

Honokiol(HK)usage is greatly restricted by its poor aqueous solubility and limited oral *** synthesized and characterized a novel phosphate prodrug of honokiol(HKP)for in vitro and in vivo *** greatly enhanced the aqueous solubility of HK(127.54±15.53 mg/ml)and the stability in buffer solution was sufficient for intravenous *** enzymatic hydrolysis of HKP to HK was extremely rapid in vitro(T 1/2=8.9±2.11 s).Pharmaco.inetics studies demonstrated that after intravenous administration of HKP(32 mg/kg),HKP was co.verted rapidly to HK with a time to reach the maximum plasma co.centration of^5 *** prodrug HKP achieved an improved T 1/2(7.97±1.30 h)and terminal volume of distribution(26.02±6.04 ml/kg)co.pared with direct injection of the equimolar parent drug(0.66±0.01 h)and(2.90±0.342 ml/kg),***,oral administration of HKP showed rapid and improved absorption co.pared with the parent *** was co.firmed to maintain the bioactivity of the parent drug for ameliorating ischemia-reperfusion injury by decreasing brain infarction and improving neurologic *** together,HKP is a potentially useful aqueous-soluble prodrug with improved pharmaco.inetic properties which may merit further development as a potential drug candidate.

关键词： Phosphate prodrug Honokiol Pharmaco.inetics Focal cerebral ischemia-reperfusion

Mitophagy in acute central nervous system injuries:regulatory mechanisms and therapeutic potentials

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2437-2453页

作者： Siyi Xu Junqiu Jia Rui Mao Xiang Cao Yun Xu Department of Neurology Nanjing Drum Tower HospitalClinical College of Jiangsu UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalChinese Academy of Medical Science&Peking Union Medical CollegeNanjingJiangsu ProvinceChina Department of Neurology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina State Key Laboratory of Pharmaceutical biotechnology and Institute of Translational Medicine for brain Critical Diseases Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Nanjing Neurology Medical Center NanjingJiangsu ProvinceChina

Acute central nervous system injuries,including ischemic stro ke,intracerebral hemorrhage,subarachnoid hemorrhage,traumatic brain injury,and spinal co.rd injury,are a major global health *** optimal therapies and improving the long-term neurological functions of patients with acute central nervous system injuries are urgent *** are susceptible to damage after acute central nervous system injury,and this leads to the release of toxic levels of reactive oxygen species,which induce cell ***,a selective form of autophagy,is crucial in eliminating redundant or damaged mitochondria during these *** evidence has highlighted the significant role of mitophagy in acute central nervous system *** this review,we provide a co.prehensive overview of the process,classification,and related mechanisms of *** also highlight the recent developments in research into the role of mitophagy in various acute central nervous system injuries and drug therapies that regulate *** the final section of this review,we emphasize the potential for treating these disorders by focusing on mitophagy and suggest future research paths in this area.

关键词： autophagy intracerebral hemorrhage ischemic stroke mitochondria mitochondrial biogenesis mitochondrial quality co.trol mitophagy spinal co.d injury subarachnoid hemorrhage traumatic brain injury

mvPPT:A Highly Efficient and Sensitive Pathogenicity Prediction Tool for Missense Variants

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2023年第2期21卷 414-426页

作者： Shi-Yuan Tong Ke Fan Zai-Wei Zhou Lin-Yun Liu Shu-Qing Zhang Yinghui Fu Guang-Zhong Wang Ying Zhu Yong-Chun Yu Jing'an District Central Hospital of Shanghai State Key Laboratory of Medical NeurobiologyMOEFrontiers Center for Brain ScienceInstitutes of Brain ScienceFudan UniversityShanghai 200032China Shanghai Xunyin biotechnology co. Ltd.Shanghai 201802China CAS Key Laboratory of co.putational Biology Shanghai Institute of Nutrition and HealthUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China Huashan Hospital State Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain ScienceInstitutes of Brain ScienceFudan UniversityShanghai 200032China

Next-generation sequencing technologies both boost the disco.ery of variants in the human genome and exacerbate the challenges of pathogenic variant *** this study,we developed Pathogenicity Prediction Tool for missense variants(mvPPT),a highly sensitive and accurate missense variant classifier based on gradient *** adopts high-co.fidence training sets with a wide spectrum of variant profiles,and extracts three categories of features,including sco.es from existing prediction tools,frequencies(allele frequencies,amino acid frequencies,and genotype frequencies),and genomic *** with established predictors,mvPPT achieves superior performance in all test sets,regardless of data *** addition,our study also provides guidance for training set and feature selection strategies,as well as reveals highly relevant features,which may further provide biological insights into variant pathogenicity.

关键词： Machine learning Missensevariant Genomics co.putational biology Pathogenicityprediction

The co.cept of gene therapy for glauco.a:the dream that has not co.e true yet

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第1期19卷 92-99页

作者： Robert Sulak Xiaonan Liu Adrian Smedowski Department of Physiology Faculty of Medical Sciences in KatowiceMedical University of Silesia in KatowiceKatowicePoland Institute of biotechnology HiLIFEUniversity of HelsinkiHelsinkiFinland Glauco.ech co. KatowicePoland

Gene therapies,despite of being a relatively new therapeutic approach,have a potential to beco.e an important alternative to current treatment strategies in *** glauco.a is not co.sidered a single gene disease,the identified goals of gene therapy would be rather to provide neuroprotection of retinal ganglion cells,especially,in intraocular-pressure-independent *** most co.monly reported type of vector for gene delivery in glauco.a studies is adeno-associated virus serotype 2 that has a high tro pism to retinal ganglion cells,res ulting in long-term expression and low immunogenic *** gene thera py studies recruit inducible and genetic animal models of optic neuropathy,like DBA/2J mice model of high-tension glauco.a and the optic nerve *** gene therapy-based neuroprotection of retinal ganglion cells is targeting specific genes translating to growth factors(i.e.,brain derived neurotrophic factor,and its receptor TrkB),regulation of apoptosis and neurodegeneration(i.e.,Bcl-xl,Xiap,FAS system,nico.inamide mononucleotide adenylyl transferase 2,Digit3 and Sarm1),immunomodulation(i.e.,Crry,C3 co.plement),modulation of neuroinflammation(i.e.,e rythropoietin),reduction of excitotoxicity(i.e.,co. KIlα)and transcription regulation(i.e.,Max,Nrf2).On the other hand,some of gene therapy studies focus on lowering intra ocular pressure,by impacting genes involved in both,decreasing aqueous humor production(i.e.,aquaporin 1),and increasing outflow facility(i.e.,co.2,prostaglandin F2a receptor,RhoA/RhoA kinase signaling pathway,MMP1,Myocilin).The goal of this review is to summarize the current stateof-art and the direction of development of gene therapy strategies for glauco.atous neuropathy.

关键词： adeno-associated virus gene editing gene therapy glauco.a IOP lowering IOP-independent mechanisms neuroprotection optic nerve optic neuropathy retinal ganglion cells

MAL2 DNA methylation serves as a biomarker for the diagnosis and prognosis of glioma

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第5期11卷 36-39页

作者： Hao Luo Xing Xiao Weiliang Hou Jing Cai Ming Chen Qisheng Tang Yusheng Tong Zengxin Qi Kaicheng Li Liang Chen Department of Neurosurgery Huashan HospitalShanghai Medical CollegeState Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain Science and Institutes of Brain ScienceFudan UniversityShanghai 200040China National Center for Neurological Disorders Shanghai 200040China Shanghai Key Laboratory of brain Function and Restoration and Neural Regeneration Shanghai 200040China Tianqiao and Chrissy Chen Institute Clinical Translational Research Center Shanghai 200040China Shanghai QuietD biotechnology co. Ltd.Shanghai 201210China

Glioma is a co.mon and malignant brain tumor,and molecular diagnostics for glioma have received increasing attention.1,2 Previous studies have suggested that the MAL2 gene may be involved in the transcytosis of various cancers.3 This study aimed to investigate the potential of MAL2 as a biomarker for *** candidate MAL2 CpG sites were validated by pyrosequencing and used to co.struct a diagnostic model for *** analysis was also co.ducted to determine the relationship between highly methylated MAL2-specific CpG sites and the prognosis of *** findings also showed that MAL2 was more highly methylated in glioma than in other *** co.structed diagnostic model can distinguish glioma from other cancers with high sensitivity(93.3%)and specificity(86.5%).Additionally,a risk sco.e model was built based on MAL2 methylation to assess the prognosis of glioma。

关键词： glioma diagnosis MAL

Primary myelofibrosis with co.current CALR and MPL mutations:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical cases 2020年第22期8卷 5618-5624页

作者： Feng-Ping Zhou Cheng-Cheng Wang Hua-Ping Du Shan-Bo Cao Jin Zhang Department of Hematology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016Zhejiang ProvinceChina Aco.nmed biotechnology co. Ltd.Beijing 100176China

BACKGROUND Primary myelofibrosis(PMF)is a myeloproliferative neoplasm(MPN)characterized by recurrent mutations in the JAK2,CALR,and MPL *** CALR and MPL co.mutation is very *** our knowledge,no more than five cases have been ***,we report a case of PMF in which a CALR and MPL co.mutation was detected by next-generation sequencing(NGS)technology,and a literature review was *** SUMMARY A 73-year-old woman was admitted to our hospital in 2018 due to abdominal *** patient had splenomegaly,lymphadenopathy,leukopenia,anemia,and immature granulocytes in peripheral *** were dacrocytes and atypical megakaryocytes in bone marrow,and megakaryocytic proliferation was very active,acco.panied by reticulin fibrosis grade *** NGS analysis of the bone marrow sample,we detected mutations in CALR,MPL,and PIK3RI,while JAK2 V617F and BCR-ABL were ***,the patient was diagnosed with PMF and received oral ***,the spleen and hematologic responses were *** review the literature,analyze previous reports of the mutation sites in our patient and differences between our patient and other reported cases of co.mutated CALR and MPL genes,and discuss the reason why the CALR and MPL co.mutations are rare and possible mechanisms and their impact on the prognosis of *** CALR and MPL mutations can be co.current in MPN,but they are *** use of NGS may help to identify more patients with co.mutated CALR and MPL *** will help to further explore the mechanism and its impact on these patients to develop appropriate treatment strategies.

关键词： Primary myelofibrosis CALR MPL co.mutation Next-generation sequencing case report

Miniaturized multico.or fluorescence imaging system integrated with a PDMS light-guide plate for biomedical investigation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

npj Flexible Electronics 2023年第1期7卷 494-503页

作者： Hyogeun Shin Gun-Wook Yoon Woongsun Choi Donghwan Lee Hoyun Choi Deok Su Jo Nakwon Choi Jun-Bo Yoon Il-Joo Cho Department of Biomedical Sciences College of MedicineKorea UniversitySeoulRepublic of Korea School of Electrical Engineering Korea Advanced Institute of Science and Technology(KAIST)DeajeonRepublic of Korea Center for brain Technology Brain Science InstituteKorea Institute of Science and Technology(KIST)SeoulRepublic of Korea biotechnology Research Institute of CHROMACH co. LtdSuwonRepublic of Korea School of Chemical Engineering Sungkyunkwan University(SKKU)SuwonRepublic of Korea KU-KIST Graduate School of co.verging Science and Technology Korea UniversitySeoulRepublic of Korea Department of Anatomy College of MedicineKorea UniversitySeoulRepublic of Korea Noul co. LtdYonginRepublic of Korea

Miniaturized fluorescence imaging systems are promising platforms that overco.e the limited use of co.ventional microsco.es in the biomedical ***,there are physical limitations for multico.or fluorescence imaging in existing miniaturized imaging systems because multiple filters have to be integrated into a small ***,we present a miniaturized multico.or fluorescence imaging system integrated with single polydimethylsiloxane(PDMS)light-guide plate(LGP)for multico.or fluorescence *** PDMS LGP allows guiding the transmitted light from the light source only to the fluorescent samples regardless of the wavelength of the light ***,our system is capable of multico.or fluorescence imaging without multiple filters that block the excitation *** demonstrated the usability of our system in the biomedical field by observing green-and red-labeled cells in the *** proposed system can be used in a wide range of applications for studies that require multico.or fluorescence imaging in the biomedical field.

关键词： structure field system

Genetic characteristics of a patient with multiple primary cancers:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical cases 2021年第28期9卷 8563-8570页

作者： Wei-Wei Ouyang Qing-Yun Li Wen-Gang Yang Sheng-Fa Su Li-Jia Wu Ying Yang Bing Lu Department of Thoracic Onco.ogy The Affiliated Hospital of Guizhou Medical University and Cancer Hospital of Guizhou Medical UniversityGuiyang 550000Guizhou ProvinceChina Genecast biotechnology co. LtdWuxi 214104Jiangsu ProvinceChina

BACKGROUND Two or multiple primary malignant neoplasms(MPMNs)rarely occur in the same *** has been reported that MPMNs are easily misdiagnosed as the recurrence or metastasis of malignancies in clinical practice,affecting the choice of treatment for the patients,thereby resulting in the delay of optimal *** generation sequencing(NGS)can be used to distinguish between multiple primary lung cancers and intrapulmonary metastasis,and may distinguish the origin of tumours in different sites of the *** SUMMARY We report the case of 66-year-old woman who suffered from different malignant neoplasms in the rectum and esophageal and gastrointestinal *** first neoplasm rectal adenocarcinoma was diagnosed and removed in *** seco.d and third lesions were diagnosed with esophageal squamous-cell carcinoma(ESCC)and gastrointestinal stromal tumour(GIST),respectively,in *** whole exome sequencing was performed on the tissue specimens of rectal carcinoma,esophageal cancer,GIST,and white blood cells to investigate the relationship between malignancies at different timeframe and determine whether the ESCC and GIST evolved from the rectal *** including v-Ki-ras2-Kirsten rat sarco.a viral onco.ene homolog,adenomatosis polyposis co.i,and mothers against decapentaplegic homolog 4 were detected in rectal adenocarcinoma sample,mast/stem cell growth factor receptor was detected in GIST tissue,and lysine methyltransferase 2D was detected in ESCC ***,ESCC and GIST were not genetically evolved from rectal adenocarcinoma,and this patient did not have a trunk driven *** NGS is an effective tool to study clonal evolution of tumours and distinguish between MPMNs and intrapulmonary metastasis.

关键词： Multiple primary malignant neoplasms Whole exome sequencing Rectal carcinoma Esophageal squamous-cell carcinoma Gastrointestinal stromal tumour case report

Programmed cell death protein-1 inhibitor co.bined with chimeric antigen receptor T cells in the treatment of relapsed refractory non- Hodgkin lymphoma: A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical cases 2021年第10期9卷 2394-2399页

作者： Zhi-Yun Niu Li Sun Shu-Peng Wen Zheng-Rong Song Lina Xing Ying Wang Jian-Qiang Li Xue-Jun Zhang Fu-Xu Wang Department of Hematology Second Hospital of Hebei Medical UniversityHebei Key Laboratory of HematologyShijiazhuang 050000Hebei ProvinceChina Central Laboratory Hebei Senlang Biotechnology Co.Shijiazhuang 050000Hebei ProvinceChina

BACKGROUND Chimeric antigen receptor T cell(CART)therapy has benefited many refractory lymphoma patients,but some patients experience poor *** studies have shown that programmed cell death protein-1(PD-1)inhibitors can improve and prolong the therapeutic effect of CAR-T cell *** SUMMARY A 61-year-old male presented with 15-d history of diarrhea and lower-limb edema.A large mass was detected in the pelvis,and pathology indicated non-Hodgkin diffuse large B-cell *** three cycles of the R-CHOP chemotherapeutic regimen,the patient showed three subcutaneous nodules under the left armpit and both sides of the cervical *** examination of the nodules indicated DLBCL *** patient was diagnosed with relapsed and refractory diffuse large B-cell *** reco.mended CAR-T cell *** treatment,the patient’s T cell function and expression of immune detection points were *** of PD-1 was obviously increased(52.7%)on cluster of differentiation(CD)3+T *** PD-1 inhibitor(3 mg/kg)was infused prior to lymphodepleting chemotherapy with fludarabine and ***-CD19 T cells of 3×10^(6)/kg and CAR-CD22 T cells 1×10^(6)/kg were infused,*** therapeutic effect was significant,and the deoxyribonucleic acid co.y numbers of CAR-CD19 T cells and CAR-CD22 T cells were ***,the patient has been disease-free for more than 12 *** This case suggests that the co.bination of PD-1 inhibitors and CAR-T cellsimproved therapeutic efficacy in B-cell lymphoma.

关键词： Chimeric antigen receptor T cell Programmed cell death protein 1 inhibitor Relapsed/refractory non-Hodgkin lymphoma case report