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Eco-toxicity and health risk assessment of polycyclic aromatic hydrocarbons in surface sediments of Burullus Lake in Egypt

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China Geology 2024年第3期7卷 460-468页

作者： Hanan E Osman Mohamed H E El-Morsy Hazem T Abd El-Hamid department of Biology Faculty of Applied ScienceUmm Al-Qura UniversityMecca 24382Saudi Arabia Deanship of Scientific Research Umm Al-Qura UniversityMakkah 24372Saudi Arabia National Institute of Oceanography and Fisheries(NIOF) Cairo 11865Egypt botany and microbiology department Faculty of ScienceAl-Azhar UniversityCairo 11651Egypt

Polycyclic aromatic hydrocarbons(PAHs)are ubiquitous environmental contaminants of growing concern due to their potential ecological and human health *** study presents a comprehensive assessment of PAHs contamination in the surface sediments of Burullus Lake,a vital and second largest delta lake in *** aim was to evaluate the eco-toxicity and potential health risks associated with the presence of these *** seven sediment samples were collected from various drains in the southern part of Burullus *** extraction method was employed to extract PAHs(16PAHs)from the sediment ***,target compounds were located using *** results showed that samples contained PAHs levels ranging from 0.038×10^(-6)to 0.459×10^(-6),which is considered heavily polluted by the European standard for PAHs ***,there was no apparent source of PAHs in the ElKhashah drain or the Brinbal Canal,as HPLC found none of the *** most prevalent compound in sediment samples along the study area was *** diagnostic indices in the present study indicated that the hydrocarbons in the region originated from pyrolytic and man-made sources along the drains of Burullus *** principal component analysis(PCA)and diagnostic ratios revealed that coal combustion and pyrolytic sources were responsible for the PAHs contamination in the surface *** non-carcinogenic risk(HI),which is the product of the HQs for the adult and child populations,respectively,was *** values under 1,therefore,demonstrated that they had no carcinogenic effects on human *** and MEQs in the sediments of Burullus Lake do not have a cancer-causing impact on *** the safety of nearby wildlife,aquatic life,and people,all activities that raise petroleum hydrocarbon levels in Burullus Lake must be adequately regulated and *** to the ecological risk assessment,there is little chance that PAHs will be found

关键词： Polycyclic aromatic hydrocarbons Diagnostic indices Toxicity Delta lake sediment Burullus Lake Human health risk Aquatic ecosystem HPLC Coal combustion

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Heavy metal pollution of river water and eco-friendly remediation using potent microalgal species

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Water Science and Engineering 2024年第1期17卷 41-50页

作者： Amudham Radha Amal Raj Prabhakaran Mylsamy V.Sivasankar B.Sathish Kumar Kiyoshi Omine T.G.Sunitha Phycological Research Laboratory Research Department of BotanyPachaiyappa's College(Affiliated to University of Madras)Chennai 600030India Research department of Chemistry Pachaiyappa's College(Affiliated to University of Madras)Chennai 600030India department of botany Sri Vidya Mandir Arts&Science College(Autonomous)KatteriUthangarai 636902India department of Civil Engineering School of Engineering.Nagasaki UniversityNagasaki-Daigaku1-14 Bunkyo-machiNagasaki 8528521Japan

Pollution of rivers is mainly caused by anthropogenic activities such as discharge of effluent from industrial facilities,maintenance of sewage/effluent treatment plants,and dumping of solid waste on river *** study dealt with the pollution issues of the Cooum River in the well-known city of Chennai in South *** samples from 27 locations were collected and analyzed for 12 elements,including Ba,B,and Al,as well as heavy metals such as Pb,Cr,Mn,Fe,Co,Ni,Cu,Zn,and *** samples showed levels of these elements that exceeded World Health Organization *** correlation analysis revealed the inter-dependency among elements,and the contribution of each element based on factor loadings showed its percentage contribution compared to *** samples from six significant locations were chosen for remediation with three algae:Chlorella vulgaris,Scenedesmus dimorphus,and Phormedium *** uptake of pollutants led to the continuous growth of algae during the incubation period of 15 d,effectively removing heavy metals from the river *** increasing levels of algal counts and the chlorophyll a content confirmed the algal growth during the incubation period,followed by a declining stage after the incubation *** scanning electron microscopic images of algae before and after the remediation showed no remarkable modification of morphological *** study showed that the uptake of heavy metals using algae is an effective water pollution remediation measure,making the process practicable in the field on a large scale in the near future.

关键词： Heavy metal Cooum river Phycoremediation Microalgae Factor analysis

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Old-growth mixed dipterocarp forests show variable losses and gains in aboveground biomass and standing carbon over forty years

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Forest Ecosystems 2024年第1期11卷 14-23页

作者： Sisira Ediriweera Champika Bandara Tithira Lakkana Shanika Jayasinghe David Woodbury Arun Dayanandan B.M.P.Singhakumara Xiangcheng Mi I.A.U.N.Gunatilleke C.V.S.Gunatilleke Mark SAshton department of Science and Technology Uva Wellassa UniversityBadullaSri Lanka The Forest School Yale School of the EnvironmentYale UniversityNew HavenCT06511USA department of Forestry and Environmental Science University of Sri JayewardenepuraNugegodaSri Lanka State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing100093China botany department University of PeradeniyaPeradeniyaSri Lanka

Background:No studies have documented long-term trends in aboveground biomass(AGB)for mixed-dipterocarp forests(MDF),the dominant rain forest type in tropical wet equatorial *** our study,we sought to document such trends over forty years across three sites representing lowland to lower montane ***:To do this,we established fifty 100 m×25 m plots in 1978 across three sites sampled along an elevation gradient,identified as mature old-growth *** measured trees for diameter at breast height that we identified to species and *** took wood samples to calculate species wood-specific *** re-measured plots in 1998 and again in ***:We show standing AGB for all sites combined to be 517.52 Mg·ha^(-1)in 1978,but this declined by 17%over 40 years to 430.11 Mg·ha^(-1).No differences exist among sites in AGB primarily because of considerable within site variation;but interactions of time with site show declines across sites were not uniform,one remained about the *** few species represented a high proportion of the AGB with the top five species comprising between 34%and 65%,depending upon site and year *** species,Mesua nagassarium,represented a disproportionately large amount of AGB and decline over time,particularly at the low elevation ***:Our results are directly relevant to estimating AGB and standing carbon sequestered in *** study is the first to demonstrate varying but overall,declining trends in amounts of AGB among forests making predictions of biomass and standing carbon in MDF difficult over wide regions.

关键词： Western ghats Shorea spp. Southeast Asia Sri Lanka Tropical rain forest Wood specific gravity

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Unveiling a new regulator: Vacuolar V-ATPase mediates brassinosteroid signaling in Arabidopsis

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Molecular Plant 2024年第2期17卷 227-229页

作者： Shikha Malik Justin W.Walley department of Plant Pathology Entomologyand Microbiologylowa State UniversityAmesIA 50011USA

Plant growth and development are intricately regulated by hormones such as auxins,gibberellins,jamonates,cytokinins,and brassinosteroids(BRs).BRs are a group of plant steroid hormones that play a vital role in plant growth and response to the *** regulate many biological processes including cell elongation,cell division,and male fertility,as well as abiotic and biotic stress responses.

关键词： media fertility brassinosteroid

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Novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan induces robust apoptosis on human acute lymphoblastic leukemia cells through a RONS-subsisted mitochondria-dependent pathway

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Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 67-77页

作者： Amrita Pal Ribhu Ray Anirban Chouni Subhadip Hajra Santanu Paul department of botany University of CalcuttaKolkata 700019India department of Cancer Chemoprevention Chittaranjan National Cancer InstituteKolkata 700026India

Objective:To explore the therapeutic effects of the novel wild edible mushroom Astraeus hygrometricus(Pers.)Morgan(***)on human acute lymphoblastic leukemia ***:Extensive screening of the antiproliferative and chemopreventive potential of different extracts from 5 wild mushrooms,***,Phallus sp.,Lentinus sp.,Tricholoma sp.,and Serpula *** performed against a panel of 6 cancer cell lines and normal cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)*** determination,cell cycle profiling,intracellular reactive oxygen species(ROS)and reactive nitrogen species(RNS),and mitochondrial membrane potential were analyzed by flow *** activity of caspases was measured colorimetrically,and the expression pattern of mitochondrial proteins was ***:The methanol extract of *** and MOLT-4 cells were identified as the most potent extract exhibiting antiproliferative activity and most sensitive cell line,*** mushroom extract induced robust selective apoptosis in MOLT-4 cells and arrested cell cycle progression at the G0/G1 *** extract disrupted the mitochondrial membrane potential and enhanced ROS production in MOLT-4 *** methanol extract induced apoptosis by downregulating the expression of Bcl-2,increasing the expression of Bax,and activating the caspase ***:The novel wild edible mushroom is a potential repository of biomolecules for the development of antileukemic drugs.

关键词： Mushroom Astraeus hygrometricus(Pers.)Morgan Apoptosis Antileukemic

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Assessment of plastic degradation by indigenous bacteria from waste disposal sites

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Emerging Contaminants 2024年第3期10卷 111-123页

作者： Farzana Javid Ghadir Ali Abdul Rehman Rehan Naeem Imran Ali Iffat Naz department of microbiology and Biotechnology Faculty of Life SciencesAbasyn University Peshawar CampusPeshawar25000Khyber PakhtunkhwaPakistan department of Life Sciences School of ScienceUniversity of Management and Technology(UMT)Lahore54700Pakistan department of microbiology Kohat University of Science and Technology(KUST)Kohat26000Khyber PakhtunkhwaPakistan department of Biotechnology and Genetic Engineering Kohat University of Science and Technology(KUST)Kohat26000Khyber PakhtunkhwaPakistan department of botany Kohat University of Science and Technology(KUST)Kohat26000Khyber PakhtunkhwaPakistan department of Biology Deanship of Educational ServicesQassim UniversityBuraydah51452Kingdom of Saudi Arabia

The present study focuses on the isolation of bacterial pathogens from waste disposal sites,and examination of their efficiencies for degrading various *** bacterial isolates such as ***,Corynebacterium spp.,Micrococcus spp.,Azotobacter spp.,Pseudomonas spp.,Staphylococcus spp.,and Bacillus *** *** *** the highest percentage reduction in black plastic weight(47.46%),while Corynebacterium spp.,and *** achieved a maximum reduction of 45.76 and 46.42%for pink plastic *** *** Micrococcus *** notable reductions of 46.43%and 56.60%in white and Canteen Stores department(CSD)biodegradable plastics,*** density(OD)measurements revealed that bacterial isolates achieved maximum OD values with CSD plastics,emphasizing their proficiency in plastic *** Transform Infrared Spectroscopy(FTIR)confirmed the degradation process,with a decrease in the peak value from 2916 to 2914 cm^(-1) for pink plastic,signifying alkane,and alkene *** new peaks at 1398,1371,and 1361 cm^(-1) in the FTIR spectrum of CSD plastic indicated presence of *** Electron Microscopic(SEM)micrographs illustrated bacterial colonization,cracks,and spots on plastic surfaces,while Xray Diffraction(XRD)spectra showed peaks at 4.083,3.705,3.020,and 1.909,suggesting plastic *** conclusion,soil of waste disposal site harbored diverse microbial species,and have highlighted their effectiveness in mitigating environmental pollution by degrading hazardous plastic waste.

关键词： Plastic degradation Bacterial isolates Waste disposal sites Fourier transform infrared spectroscopy Scanning electron microscopy X-ray diffraction Environmental pollution

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Seed Priming with Potassium Nitrate Can Enhance Salt Stress Tolerance in Maize

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Phyton-International Journal of Experimental botany 2024年第8期93卷 1819-1838页

作者： Bushra Rehman Asma Zulfiqar Houneida Attia Rehana Sardar Muneera A.Saleh Khalid H.Alamer Ibtisam M.Alsudays Faisal Mehmood Qamar uz Zaman Institute of botany University of the PunjabLahore54590Pakistan department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia department of botany Emerson UniversityMultan60000Pakistan Biological Sciences department Faculty of Science and ArtsKing Abdulaziz UniversityRabigh21911Saudi Arabia department of Biology College of ScienceQassim UniversityBurydah52571Saudi Arabia department of Chemistry Sciences The University of LahoreLahore54590Pakistan department of Environmental Sciences The University of LahoreLahore54590Pakistan

Salinity is a major abiotic stress that hinders plant development and productivity and influences agricultural *** priming is a technique used to boost germination and seedling growth under abiotic stress.A pot experiment was conducted to evaluate the impact of seed priming with potassium nitrate(KNO_(3))at various levels(0%,0.50%,1.00%and 1.50%)under salt stress(0,75,100 mM NaCl)on two maize verities(MNH360 and 30T60)for the growth,development and metabolic attributes results revealed that in maize variety MNH360,KNO_(3)priming’s significantly enhanced growth parameters than in maize variety 30T60 under control and salt-stressed *** with KNO_(3)enhanced carotenoids and total chlorophyll in the 30T60 variety that protected the maize plants from salt ***,it was shown that priming with 1.00%KNO_(3)acts as safeguarded to protect them from oxidative damage by salt stress minimizing reactive oxygen species(ROS)formation through increased levels of malondialdehyde(MDA),catalase(CAT),peroxidase(POD),ascorbate peroxidase(APX),and total soluble *** findings of the present study confirm that the use of the KNO_(3)seed priming technique is a lowcost,environmentally friendly technique for mitigating adverse impacts of salt stress in maize crops by activating the antioxidant defense system and improving chlorophyll and osmolyte contents.

关键词： Salinity Zea mays antioxidant enzymes growth seedlings oxidative damage

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Foliar spraying of chlorpyrifos induces morphometric changes in Glycine max(L.)and shifts native soil microbiome

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Emerging Contaminants 2024年第3期10卷 86-102页

作者： Aman Raj Muneer Ahmad Malla Ashwani Kumar Pramod Kumar Khare Sheena Kumari Metagenomics and Secretomics Research Laboratory Department of BotanyDr.Harisingh Gour University(A Central University)Sagar470003M.P.India Institute for Water and Wastewater Technology Durban University of TechnologyDurban4001South Africa Metagenomics and Secretomics Research Laboratory Department of BotanyUniversity of Allahabad(A Central University)Prayagraj211002U.P.India department of botany Ecology laboratoryDr.Harisingh Gour Vishwavidyalaya(A Central University)SagarMadhya Pradesh470003India

Pesticide usage alters plant growth,and development and disturbs native soil *** present study was carried out to evaluate the effect of Chlorpyrifos(CP-20%EC)on the growth and development of soybean(Glycine max),soil fertility,and soil *** conducted a pot experiment to assess the impact of different concentrations of chlorpyrifos;recommended dose(RD)and higher dose rates(2×and 4×RD).Chlorpyrifos application significantly altered the growth parameters in soybean(p<0.05).At higher concentrations(2×and 4×RD)shoot length(17.16±1.04;14.33±1.15,p<0.05),root length(3.40±0.36;2.66±0.28,p<0.050),leaf count(6.33±0.57;2.66±0.57;p<0.05)reduced significantly compared to RD and Control *** at these concentrations(2×and 4×RD),significant reduction in fresh shoot weight(0.86±0.008;0.66±0.002,p<0.05),dry shoot weight(0.14±0.001;0.13±0.003,p<0.05)and root fresh weight(0.13±0.001;0.09±0.003,p<0.05)and root dry weight(0.030±0.0005,0.037±0.003,p<0.05)was observed compared to the RD and control ***,a significant increase in root and shoot morphometric parameters was observed in RD compared to control(p<0.05).All the physio-chemical properties such as pH(8.51±0.82),moisture content(33.73±0.04),and electrical conductivity(3.44±0.03)were higher in CP-treated soils compared to ***,nutrient content such as nitrogen,carbon,and hydrogen were significantly reduced in treated soils compared to *** analysis showed higher concentrations of As,Cd,and Pb in CP-treated soil(8.7 mg/g,0.254 mg/g,and 16.477 mg/g,respectively)compared to untreated soil(8.09 mg/g,0.228 mg/g,and 15.25 mg/g,respectively).We also assessed soil metabolic activity and diversity using Community-level Physiological Profiling(CLPP).CP-treated soil exhibited significantly lesser microbial diversity,with lesser metabolic *** analysis revealed a shift in bacterial community composition,with Pseudomonadota and Bacteroidota dominating CP-treated s

关键词： Chlorpyrifos Glycine max Proline Metabolic activity CLPP Metagenomics Bacterial microbiome

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A critical review of microplastics in aquatic ecosystems: Degradation mechanisms and removing strategies

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Environmental Science and Ecotechnology 2024年第5期21卷 62-76页

作者： Sameh S.Ali Tamer Elsamahy Rania Al-Tohamy Jianzhong Sun Biofuels Institute School of the Environment and Safety EngineeringJiangsu UniversityZhenjiang212013China botany department Faculty of ScienceTanta UniversityTanta31527Egypt

Plastic waste discarded into aquatic environments gradually degrades into smaller fragments,known as microplastics(MPs),which range in size from 0.05 to 5 *** ubiquity of MPs poses a significant threat to aquatic ecosystems and,by extension,human health,as these particles are ingested by various marine organisms including zooplankton,crustaceans,and fish,eventually entering the human food *** contamination threatens the entire ecological balance,encompassing food safety and the health of aquatic ***,developing effective MP removal technologies has emerged as a critical area of ***,we summarize the mechanisms and recently reported strategies for removing MPs from aquatic *** combining physical and chemical pretreatments with microbial degradation have shown promise in decomposing *** such as bacteria,fungi,algae,and specific enzymes are being leveraged in MP remediation *** advancements have focused on innovative methods such as membrane bioreactors,synthetic biology,organosilane-based techniques,biofilm-mediated remediation,and nanomaterial-enabled strategies,with nano-enabled technologies demonstrating substantial potential to enhance MP removal *** review aims to stimulate further innovation in effective MP removal methods,promoting environmental and social well-being.

关键词： Persistent organic pollutants Bioremediation Nanomaterial-enabled strategies Aquatic environment Ecotoxicity of microplastics

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Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot

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aBIOTECH 2024年第2期5卷 151-168页

作者： Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat Kumar Tripathy Priya Das Sagar Banerjee Yiping Qi Sanghamitra Samantaray Pradipta Kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla ICAR National Rice Research Institute CuttackOdisha 753006India department of botany Ravenshaw UniversityCuttackOdisha 753003India department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA

The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic ***-predicted guide RNAs(gRNAs)often fail to induce efficient cleavage at target *** target loci are inaccessible due to complex chromatin ***,there is no suitable tool available to predict the architecture of genomic target sites and their ***,significant time and resources are spent on performing editing experiments with inefficient *** in vitro-cleavage assay could provide a rough assessment of gRNA efficiency,it largely excludes the interference of native genomic *** in-vivo testing gives a proper assessment of the cleavage ability of editing reagents in a native genomic ***,we developed a modified protocol that offers highly efficient protoplast isolation from rice,Arabidopsis,and chickpea,using a sucrose gradient,transfection using PEG(polyethylene glycol),and validation of single guide RNAs(sgRNAs)cleavage efficiency of *** have optimized various parameters for PEG-mediated protoplast transfection and achieved high transfection efficiency using our protocol in both monocots and *** introduced plasmid vectors containing Cas9 and sgRNAs targeting genes in rice,Arabidopsis,and chickpea *** dual sgRNAs,our CRISPRdeletion strategy offers straightforward detection of genome editing success by simple agarose gel *** sequencing of PCR products confirmed the editing efficiency of specific ***,we demonstrated that isolated protoplasts can be stored for up to 24/48 h with little loss of viability,allowing a pause between isolation and *** high-efficiency protocol for protoplast isolation and transfection enables rapid(less than 7 days)validation of sgRNA cleavage efficiency before proceeding with stable *** isolation and transfection method can als

关键词： Protoplast Genome editing Transgene-free edited plants Rice Chickpea

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