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A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos P.J.Dijkstra C.Mota J.Bauer H.Ferreira Rui L.Reis N.Neves S.Camarero-Espinosa M.B.Baker L.Moroni Department of Complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s research group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PCL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PCL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMSCs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor II(BMPRII)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density Click chemistry Human

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CT Thresholds for Spinal bone Mineral Attenuation to Prioritize Eligible Older Women for Osteoporosis Screening

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Open Journal of Radiology 2024年第2期14卷 92-101页

作者： Seo Yeon K. Orite Bryn Higuchi Hyo-Chun Yoon Lana H. Gimber St. Elizabeths Medical Center Brighton MA USA University of California Irvine CA USA Kaiser Permanente Division of research Oakland CA USA Department of Diagnostic Imaging Hawaii Permanente Medical Group Honolulu HI USA

Introduction: Computed tomography (CT) measurements of bone mineral attenuation may be a useful means to identify older women who should be prioritized for bone mineral density screening. Methods: We compared bone mineral attenuation as measured in the L1 vertebra of CT studies to the results of dual-energy x-ray absorptiometry (DEXA) studies to determine what CT attenuation thresholds might yield a reasonable combination of sensitivity and specificity for the detection of osteoporosis. The study was limited to women between the ages of 65 and 75 years who had a DEXA study and a CT that included the L1 or adjacent vertebra performed within 3 years of the DEXA study. Results: There were 1226 women in this study, of whom 452 (38%) had osteoporosis based on a T-score ≤ −2.5 by DEXA. There were 830 CT studies performed with contrast and 396 studies which were performed without contrast. There was a statistically significant difference in the mean HU of those studies performed without contrast compared to those with contrast (unenhanced mean 103 HU versus 125 HU, p < 0.001). Different CT attenuation thresholds provided the most appropriate combination of sensitivity and specificity for the detection of osteoporosis when comparing CT studies performed without or with IV contrast and when all the CT data were used in aggregate. Conclusion: Different thresholds appear necessary when using the mean CT vertebral attenuation to identify older women for preferential referral for DEXA studies.

关键词： Osteoporosis bone Mineral Attenuation CT Threshold

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AN EFFECT OF REGIONAL FOOD COMPOSITION ON THE bone MINERAL density

AN EFFECT OF REGIONAL FOOD COMPOSITION ON THE BONE MINERAL D...

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第二届国际骨质疏松研讨会

作者： Yan Ling Yan Zhongfu Muo Yuoring bone density research group Radiation divisionNational Institute of Measurement and Testing Technology4Beier xiangShuinianheChengdu 610061P.R.China Guankou Hospital

The present paper describes the effect of composition of two kinds of different natural food on the bone mineral density(BMD).Both kinds of food are the principal food for peasants in Guankou district in the west of Sichuan province,one of which is the coarse food grain and cereals eaten by peasants in hilly region,which the other one is husked rice eaten by peasants in plain area,bone mineral density to peasants over 40 years old from the above two areas has been measured by using Am single photon bone mineral *** BMD for male peasants between 40～65 years old from hilly area is(0.81±0.09) g/cm,while that from plain area is(0.78±0.08) g/cm,The former figure is obviously higher than the latter one(pbone mineral density of human body.

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Effect of esculetin on bone metabolism in ovariectomized rats

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Journal of Traditional Chinese Medicine 2018年第6期38卷 896-903页

作者： Liu Meijie Wang Ruihai Li Yan Bai Dong Pan Jinghua Liu Hong Wang Shaojun Wu Jiaying Sun Gang Miao Qing Ju Dahong Liu Limei bone and Joint Diseases Laboratory Experimental Research CenterChina Academy of Chinese Medicine Science 100700China The Basic research Center of Recipe and Syndrome in Traditional Chinese Medicine Institute of Basic Theory for Chinese MedicineChina Academy of Chinese Medicine ScienceBeijing 100700China

OBJECTIVE: To determine the effect of an esculetin formulation(at 97.4% purity) on osteoporosis, and to investigate the potential underlying molecular mechanism(s).METHODS: Sixty specific pathogen free-grade female Wistar rats were randomly assigned to three groups: blank control(n = 12), sham(n = 12), and model(n = 36). The model group were bilaterally ovariectomized. The sham group had the tissue surrounding the ovaries removed, while the ovaries were retained. After 3 months, the model group was randomly divided into three subgroups: OVX(n = 12), positive control(n = 12), and esculetin(n =12). The positive control group and the esculetin group were intragastrically administered diethylstilbestrol(0.046 mgkd^(-1)), respec·kg^(-1)tively,·d^(-1)) or esculetin(384 mgg^(-1) once per day for 6 consecu··-tive days; medication administration was then stopped for 1 d, before being administered for another 6 consecutive days. All rats were treated for 3months. Samples were collected at the end of the treatment period. An Osteocore3 Digital 2D bone densitometer was used to test the bone mineral density, and histomorphometric analysis was performed to measure bone mass, bone formation,and bone resorption. Enzyme-linked immunosorbent assay analysis was used to measure the serum concentrations of interleukin-6(IL-6), osteoprotegerin(OPG), and receptor activator of nuclear factor-kappa B ligand(RANKL). Immunohistochemistry and in situ hybridization were performed to detect the protein and mRNA expressions of OPG and RANKL in osteoblasts and bone marrow stromal ***: Compared with the OVX group, the esculetin group had significantly greater femoral bone mineral density and tibial trabecular bone volume,and significantly smaller trabecular resorption surface. The percentage of trabecular formation surface, average osteoid width, trabecular bone mineralization rate, and cortical bone mineralization rate did not significantly differ between groups. Compared with the sham g

关键词： Esculetin Osteoporosis Osteoprotegerin RANK ligand bone resorption

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Assessment of volumetric bone mineral density of the femoral neck in postmenopausal women with and without vertebral fractures using quantitative multi-slice CT

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第7期10卷 499-504页

作者： Sheng-yong WU Hui-hui JIA Didier HANS Jing LAN Li-ying WANG Jing-xue LI Yue-zeng CAI Department of Radiology Tianjin First Central Hospital Tianjin 300192 China Department of Radiology General Hospital of Tianjin Medical University Tianjin 300052 China Center of bone Diseases Department of bone and Joint Lausanne University Hospital 1011 Lausanne Switzerland

Objective： To demonstrate the validity and reliability of volumetric quantitative computed tomography （vQCT） with multi-slice computed tomography （MSCT） and dual energy X-ray absorptiometry （DXA） for hip bone mineral density （BMD） measurements, and to compare the differences between the two techniques in discriminating postmenopausal women with osteoporosis-related vertebral fractures from those without. Methods： Ninety subjects were enrolled and divided into three groups based on the BMD values of the lumbar spine and/or the femoral neck by DXA. groups 1 and 2 consisted of postmenopausal women with BMD changes 〈-2SD, with and without radiographically confirmed vertebral fracture （n= 11 and 33, respectively）. group 3 comprised normal controls with BMD changes 〉-ISD （n-46）. Post-MSCT （GE, LightSpeed16） scan reconstructed images of the abdominal-pelvic region, 1.25 mm thick per slice, were processed by OsteoCAD software to calculate the following parameters： volumetric BMD values of trabecular bone （TRAB）, cortical bone （CORT）, and integral bone （INTGL） of the left femoral neck, femoral neck axis length （NAL）, and minimum cross-section area （mCSA）. DXA BMD measurements of the lumbar spine （AP-SPINE） and the left femoral neck （NECK） also were performed for each subject. Results： The values of all seven parameters were significantly lower in subjects of groups 1 and 2 than in normal postmenopausal women （P〈0.05, respectively）. Comparing groups 1 and 2, 3D-TRAB and 3D-INTGL were significantly lower in postmenopausal women with vertebral fracture（s） [（109.8±9.61） and （243.3±33.0） mg/cm^3, respectively] than in those without [（148.9±7.47） and （285.4±17.8） mg/cm^3, respectively] （P〈0.05, respectively）, but no significant differences were evident in AP-SPINE or NECK BMD. Conclusion： the femoral neck-derived volumetric BMD parameters using vQCT appeared better than the DXA-derived ones in discriminating osteoporotic postmenopausal women with vertebral fractures from those wit

关键词： Osteoporosis bone mineral density (BMD) Volumetric QCT Hip fracture Postmenopausal women

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Plyometric exercise and bone health in children and adolescents: a systematic review

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World Journal of Pediatrics 2017年第2期13卷 112-121页

作者： Alejandro Gómez-Bruton (A)ngel Matute-Llorente Alejandro González-Agüero JoséA.Casajús Germán Vicente-Rodríguez Growth ExerciseNutrition and Development(GENUD)Research GroupUniversity of ZaragozaZaragozaSpain Faculty of Health and Sport Sciences(FCSD) Department of Physiatry and NursingUniversity of ZaragozaRonda Misericordia 522001-HuescaSpain Growth ExerciseNutrition and Development(GENUD)Research GroupUniversity of ZaragozaZaragozaSpain

Background:Many jumping interventions have been performed in children and adolescents in order to improve bone-related variables and thus,ensure a healthy bone development during these periods and later in *** systematic review aims to summarize and update present knowledge regarding the effects that jumping interventions may have on bone mass,structure and metabolism in order to ascertain the efficacy and durability (duration of the effects caused by the intervention) of the *** sources:Identification of studies was performed by searching in the database MEDLINE/PubMed and *** studies were identified by contacting clinical experts and searching bibliographies and *** terms included 'bone and bones','jump*','weight-bearing','resistance training' and 'school intervention'.The search was conducted up to October *** studies that had performed a specific jumping intervention in under 18-year olds and had measured bone mass were *** extraction of articles was done by 2 authors using predefined data ***:A total of 26 studies were included in this ***-four studies found positive results as subjects included in the intervention groups showed higher bone mineral density,bone mineral content and bone structure improvements than *** two studies found no effects on bone mass after a 10-week and 9-month ***,those studies that evaluated the durability of the effects found that some of the increases in the intervention groups were maintained after several ***:Jumping interventions during childhood and adolescence improve bone mineral content,density and structural properties without side *** type of interventions should be therefore implemented when possible in order to increase bone mass in early stages of life,which may have a direct preventive effect on bone diseases like osteoporosis later in fife.

关键词： adolescents bone mineral density intervention jumping osteoporosis

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Tailoring the degradation rate of magnesium through biomedical nano-porous titanate coatings

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Journal of Magnesium and Alloys 2021年第1期9卷 336-350页

作者： Matthew D.Wadge Jamie McGuire Benjamin V.T.Hanby Reda M.Felfel Ifty Ahmed David M.Grant Advanced Materials research group Faculty of EngineeringUniversity of NottinghamNG72RD United Kingdom Physics Department Faculty of ScienceMansoura UniversityMansoura 35516Egyp

A novel approach was developed to reduce the corrosion rate of magnesium(Mg)metal,utilising titanate *** sputtering was used to deposit ca.500 nm titanium(Ti)coatings onto pure Mg discs,followed by hydrothermal conversion and ion exchange reactions to produce sodium and calcium titanate *** confirmed the characteristic nanoporous structure of sodium and calcium titanate,with thicknesses ranging from ca.0.8 to 1.4μ*** analysis confirmed the presence of Ti^(4+)-O,Na-O,and Ca-O bonding,whilst Raman spectroscopy demonstrated characteristic vibrational modes(such as TiO 6 octahedral vibrations)of the sodium and calcium titanate perovskite ***,corrosion studies through potentiodynamic polarisation measurements demonstrated the NB/NH CaTC samples to be superior in reducing Mg degradation,compared to other samples tested,through an increase in E_(corr)from-1.49 to-1.33 V,and the reduction in corrosion current density,i corr,from 0.31 to 0.06 mA/cm^(2)for Mg and NB/NH CaTC samples,*** was a clear trend noted for the NB/NH samples,which showed an increase in E corr to more positive values in the following order:Mgbone fracture fixation,or other orthopaedic applications.

关键词： Magnesium degradation Titanate Biodegradable Ion exchange Electrochemical corrosion Magnetron sputtering

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Exploring the effects of taurolidine on tumor weight and microvessel density in a murine model of osteosarcoma

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Oncology research 2024年第7期32卷 1163-1172页

作者： LISANNE K.A.NEIJENHUIS LEUTA L.NAUMANN SONIA A.M.FERKEL SAMUEL J.S.RUBIN STEPHAN ROGALLA Division of Gastroenterology and Hepatology Department of MedicineSchool of MedicineStanford UniversityStanford94305USA Department of Surgery Leiden University Medical CenterLeiden2333 ZAThe Netherlands Centre for Human Drug research Leiden2333 CLThe Netherlands Department of General VisceralVascular and Thoracic SurgeryCharite University Medicine BerlinBerlin10117Germany

Background:Osteosarcoma is the most common malignant primary bone *** prognosis for patients with disseminated disease remains very poor despite recent advancements in ***,current treatment regimens bear a signiﬁcant risk of serious side ***,there is an unmet clinical need for effective therapies with improved safety proﬁ*** is an antibacterial agent that has been shown to induce cell death in different types of cancer cell ***:In this study,we examined both the antineoplastic and antiangiogenic effects of taurolidine in animal models of osteosarcoma.K7M2 murine osteosarcoma cells were injected,both intramuscular and intraperitoneal,into 60 BALB/c mice on day *** were then randomized to receive treatment with taurolidine 2%(800 mg/kg),taurolidine 1%(400 mg/kg),or NaCl 0.9%control for seven days by intravenous or intraperitoneal ***:After 35 days,mice were euthanized,and the tumors were harvested for *** mice were excluded from the analysis due to *** weight was signiﬁcantly lower in the 2%taurolidine intraperitoneal treatment group from day 9 to 21,consistent with elevated mortality in this *** tumor weight was signiﬁcantly lower in the 1%(p=0.003)and 2%(p=0.006)intraperitoneal taurolidine treatment groups compared to the *** antineoplastic effects were observed on intramuscular tumors or for intravenous administration of *** were no signiﬁcant differences in microvessel density or mitotic rate between treatment *** body weight and elevated mortality in the 2%taurolidine intraperitoneal group suggest that the lower 1%dose is ***:In conclusion,there is no evidence of antiangiogenic activity,and the antitumor effects of taurolidine on osteosarcoma observed in this study are ***,its toxic proﬁle grants further *** these observations,further research is necessary to

关键词： Osteosarcoma Taurolidine Cancer treatment Chemotherapy Murine models

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Exploring the multicomponent synergy mechanism of Zuogui Wan(左归丸) on postmenopausal osteoporosis by a systems pharmacology strategy

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Journal of Traditional Chinese Medicine 2024年第3期44卷 489-495页

作者： FENG Yanchen LIU Yali DANG Xue LIN Zixuan ZHANG Yunke CHE Zhiying LI Xiang PAN Xiaolong LIU Feixiang ZHENG Pan Traditional Chinese Medicine(Zhong Jing)School Henan University of Chinese MedicineZhengzhou 450046China Hospital of Encephalopathy the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 450000China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseasesthe First Affiliated HospitalZhejiang University School of MedicineResearch Units of Infectious Disease and MicroecologyChinese Academy of Medical SciencesHangzhou 31000China

OBJECTIVE: To explore the multi-component synergistic mechanism of Zuogui Wan(左归丸, ZGW) in treating postmenopausal osteoporosis(PMOP). METHODS: The main components and target genes of ZGW were screened via the Traditional Chinese Medicine Systems Pharmacology(TCMSP). In addition, the target gene sets of PMOP were derived from the Gene Cards and Online Mendelian Inheritance in Man databases. The search tool for recurring instances of neighbouring genes(STRING) 11.0 software was used to analyze the interaction among intersecting genes. Cytoscape 3.6.1 software and the Matthews correlation coefficient(MCC) algorithm were used to screen the core genes. Fifty Sprague-Dawley female rats were randomly divided into the sham-operated(Sham) group and the four ovariectomized(OVX) subgroups. Rats subjected to Sham or OVX were administered with the vehicle(OVX, 1 m L water/100 g weight), 17β-estradiol(E2, 50 μg·kg-1·d-1), and lyophilized powder of ZGW at a low dose of 2.3(ZGW-L) and high dose of 4.6(ZGW-H) g·kg-1·d-1 for three months. The bone density and bone strength were assessed using dual-energy X-ray and three-point bending tests, respectively. Furthermore, enzyme-linked immunosorbent assay, Hematoxylin-eosin staining, and western blot analysis were used to determine the potential pharmacological mechanisms of action of ZGW in PMOP. RESULTS: A total of 117 active compounds of ZGW were screened from the TCMSP. Furthermore, 108 intersecting genes of drugs and diseases were identified. Using STRING software and the MCC algorithm, ten core genes, including C-X-C chemokine living 8(CXCL8), C-C chemokine receptor type 2(CCR2), alpha-2a active receptor(ADRA2A), melatonin receptor type 1B(MTNR1B), and amyloid-beta A4 protein(APP), were identified. The anti-osteoporosis regulation network of ZGW was constructed using the Cytoscape software. The animal experiments demonstrated that ZGW groups significantly reduced the serum levels of β-C-terminal telopeptide of type I collagen(β-CTX

关键词： osteoporosis postmenopausal network pharmacology Zuogui Wan

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p-Coumaric acid alleviates adriamycin-induced hepatotoxicity in rats

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Asian Pacific Journal of Tropical Biomedicine 2021年第3期11卷 115-121页

作者： Zeinab Rafiee Maasoumeh Zare Moaiedi Armita Valizadeh Gorji Esrafil Mansouri Student research Committee Ahvaz Jundishapur University of Medical SciencesAhvazIran Department of Clinical Biochemistry Faculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran bone Marrow Transplantation Center Ahvaz Jundishapur University of Medical SciencesAhvazIran Cellular and Molecular research Center Department of Anatomical SciencesFaculty of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran

Objective:To evaluate the effect of p-coumaric acid against adriamycin-induced hepatotoxicity in ***:The rats were divided into 4 *** control group received solvent;the p-coumaric acid group was treated with 100 mg/kg of p-coumaric acid orally for five consecutive days;the adriamycin group was administered with a single dose of adriamycin(15 mg/kg,i.p.),and the p-coumaric acid+adriamycin group was given p-coumaric acid five days before adriamycin ***-four hours after the last administration,blood samples were collected for biochemical analysis,and liver tissues were removed for histopathological and immunohistochemistrical ***,the levels of tissue lipid peroxidation and enzyme activities of glutathione peroxidase,superoxide dismutase,and catalase in liver tissue were ***:Treatment with p-coumaric acid protected the liver from the toxicity of adriamycin by attenuating the increase in alkaline phosphatase,alanine transaminase,aspartate transaminase,total bilirubin,total cholesterol,triglyceride,and low-density lipoprotein cholesterol and lessening the decrease in high-density lipoprotein cholesterol and albumin.p-Coumaric acid also raised the levels of glutathione peroxidase,superoxide dismutase,and catalase,as well as decreased lipid peroxidation in liver tissue and hepatic IL-1β***,histopathological study confirmed the protective effect of p-coumaric acid against liver ***:p-Coumaric acid can alleviate adriamycin-induced hepatotoxicity.

关键词： Adriamycin Hepatotoxicity p-Coumaric acid Antioxidant IL-1β

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