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Chinese Science Bulletin 2013年第13期58卷 1558-1567页

作者： ZHAO XiangXiang TANG Tang CHEN GuiMin LIU FuXia WANG XingLong BU CuiPing LU ChangMing key laboratory of Oil Crop Biology of Ministry of agriculture Oilcrops Research InstituteChinese Academy of Agricultural Sciences Jiangsu key laboratory for eco-Agricultural biotechnology around hongze lake Huaiyin Normal University

The isolation distance required for field trials of genetically modified (GM) rapeseed varies widely worldwide, with a 50-400 m distance in most nations contrasting with a minimum 1000-m isolation distance in China. The goal of this study was to evaluate the relevance of current regulations in China regarding the isolation distance needed for GM rapeseed trials. A pollen flow experiment was conducted based on the design of concentric circles, with the GM plants in a 20-m diameter circle at the centre, surrounded by non-GM plants to a distance 60 m from the perimeter of the circle containing GM plants. The rate of pollen flow was the highest at the isolation distance of 0.5 m, where it ranged from 2.3091% to 2.6711%. The general pattern of the pollen flow rate (y) with distance (x) was well described by the equation y = 1.3936x-0.9136 (R 2 = 0.9950). The long-distance pollen flow tested at the isolation distance of 800 m was 0.0018%, which agrees with the theoretical prediction. The results suggested that 300 m, rather than 1000 m, is a reasonable distance to ensure a tolerable threshold of pollen flow (less than 0.01%) under conditions of winter rapeseed production in China.

关键词：转基因油菜籽隔离距离现场试验中国转基因植物花粉流世界范围田间试验

Gene flow from transgenic oilseed rape (Brassica napus L.) to cruciferous weeds under mentor pollen inducement

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Progress in Natural Science:Materials International 2007年第11期17卷 1284-1289页

作者： Zhao Xiangxiang1,2, Luo Yuming1, Lu Weiping2, Qi Cunkou3, Pu Huiming3 and Wang Youping2(1. biotechnology key laboratory of eco-agriculture in hongze lake region, Jiangsu Province, Huaiyin Normal College, Huai'an 223001, China 2. Jiangsu Provincial key laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China 3. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China) biotechnology key laboratory of eco-agriculture in hongze lake region Jiangsu Province Huaiyin Normal College Huai'an 223001 China Jiangsu Provincial key laboratory of Crop Genetics and Physiology Yangzhou University Yangzhou 225009 China Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China

The alien gene flow between genetically modified glyphosate-resistant rapeseed variety Q3 (Brassica napus L.) and four cruciferous weeds was studied under mentor pollen inducement. The results showed that when Thlaspi arvense L., Capsella bursa-pastoris (L.) Medic, Cardamine hirsuta L. and Rorippa palustris (L.) Besser were pollinated with mentor pollen, the mixed Q3 and the weed, pollen grains aggregated largely and germinated quickly, and the numbers of pollen tubes penetrating into the style and the ovary were greatly increased as compared with corresponding self-pollination groups. Twenty four to forty eight hours after pollination, several pollen tubes were observed to penetrate into the ovule via micropyle in each mentor combination. However, when the mentor progenies were analyzed by PCR, all of them showed negative for the Q3 herbicide-resistant gene. Collectively, these results indicated that crossing between T. arvense, C. bursa-pastoris, C. hirsuta, R. palustris (as female) and Q3 (as male) was highly incompatible and the herbicide-resistant gene could not flow from Q3 to these four weeds.

关键词： herbicide-resistant rapeseed cruciferous weeds mentor pollination aniline blue fluorescence gene flow

A rice geranylgeranyl reductase is essential for chloroplast development

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Journal of Integrative agriculture 2021年第10期20卷 2592-2600页

作者： LIU Xi YI Xin YANG Yan-rong HUANG Qian-qian Jiangsu Collaborative Innovation Center of regional Modern agriculture and Environmental Protection/Jiangsu key laboratory for eco-Agricultural biotechnology Around hongze lake Huaiyin Normal UniversityHuai’an 223300P.R.China

Chloroplasts are essential for plant photosynthesis and *** genes have been identified that regulate plant chloroplast ***,it is not known at a molecular level how these genes regulate chloroplast *** this study,we isolated a mutant ygl2(yellow-green leaf2)that exhibited a pigment-defective ***2 encodes a geranylgeranyl reductase,and in mutant ygl2,there was a single base change(T1361G)located in the third exon of YGL2 that resulted in a missense mutation(L454R)in the encoded *** electron microscopy revealed that chloroplast development was impaired in the ygl2 *** expression levels of plastid-encoded genes were significantly altered in the ygl2 ***,in a yeast two-hybrid assay,we found that YGL2 interacted with the RNA editing factor MORF8.

关键词： rice(Oryza sativa L.) chloroplast YGL2 geranylgeranyl reductase

OsMADS32 interacts with PI-like proteins and regulates rice flower development

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Journal of Integrative Plant Biology 2015年第5期57卷 504-513页

作者： Huanhuan Wang Liang Zhang Qiang Cai Yun Hu Zhenming Jin Xiangxiang Zhao Wei Fan Qianming Huang Zhijing Luo Mingjiao Chen Dabing Zhang Zheng Yuan State key laboratory of Hybrid Rice School of Life Sciences and BiotechnologyShanghai Jiao Tong University College of Life and Basic Sciences Sichuan Agricultural University Jiangsu key laboratory for eco-Agricultural biotechnology around hongze lake Huaiyin Normal University

OsMADS32 is a monocot specific MIKCc type MADS‐box gene that plays an important role in regulating rice floral meristem and organs identity, a crucial process for reproductive success and rice yield. However, its underlying mechanism of action remains to be clarified. Here, we characterized a hypomorphic mutant allele of OsMADS32/CFO1, cfo1‐3 and identified its function in controlling rice flower development by bioinformatics and protein‐protein interaction analysis. The cfo1‐3 mutant produces defective flowers, including loss of lodicule identity, formation of ectopic lodicule or hull‐like organs and decreased stamen number, mimicking phenotypes related to the mutation of B class genes. Molecular characterization indicated that mis‐splicing of OsMADS32 transcripts in the cfo1‐3 mutant resulted in an extra eight amino acids in the K‐domain of OsMADS32 protein. By yeast two hybrid and bimolecular fluorescence comple-mentation assays, we revealed that the insertion of eight amino acids or deletion of the internal region in the K1 subdomain of OsMADS32 affects the interaction between OsMADS32 with PISTILLATA （PI）‐like proteins OsMADS2 and OsMADS4. This work provides new insight into the mecha-nism by which OsMADS32 regulates rice lodicule and stamen identity, by interaction with two PI‐like proteins via its K domain.

关键词： Floral organ identity hypomorphic mutant K domain OsMADS32 protein interaction

Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach

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Journal of Integrative agriculture 2013年第11期12卷 2013-2021页

作者： ZHAO Xiang-xiang TANG Tang LIU Fu-xia LU Chang-li HU Xiao-lan JI Li-lian LIU Qiao-quan Jiangsu key laboratory for eco-Agricultural biotechnology Around hongze lake Huaiyin Normal University key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics Ministry of Education/Yangzhou University

Development of new technologies for evaluating genetically modiifed （GM） crops has revealed that there are unintended insertions and expression changes in GM crops. Proifling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein proifle differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis （2-DE） and mass spectrum analysis of the seeds identiifed 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.

关键词： transgenic rice proteome profiling techniques unintended changes two-dimensional gel electrophoresis

Molecular Cloning and Expression Analysis of a FT Homologous Gene from Solanum tuberosum

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Agricultural Sciences in China 2010年第8期9卷 1133-1139页

作者： FAN Chun-yuan YIN Jing-ming WANG Bing ZHANG Yun- feng YANG Qing State key laboratory of Crop Genetics and Germplasm Enhancement/College of Life Sciences Nanjing Agricultural University Nanjing 210095 P.R.China Jiangsu key laboratory for eco-Agricultural biotechnology Around hongze lake Huai 'an 223300 P.R.China

A homologue of flowering locus T gene, designated StFT, was isolated from Solanum tuberosum by reverse transcriptasepolymerase chain reaction （accession no. GU223211）. The DNA sequence of StFT was 1 626 bp long and contained four exons and three introns. The open reading frame of the gene was 522 bp long and encoded a putative protein of 173 amino acids with a molecular weight of 19.75 kD and a theoretical pI of 7.76. StFT protein had a conserved PBP domain and a higher degree of identity with FT homologous members from other species. Analysis on the mRNA levels of StFT showed that it was highly expressed in leaves, apical buds, flowers, and swelling stolons. Further analysis indicated that its expression was regulated by CONSTANS gene in StCOL-antisense transgenic potato plants.

关键词： cloning expression StFT gene Solanum tuberosum

Lack of Tocopherol Inhibits Rice Growth by Triggering an Ectopic Stress Response and the Accumulation of DELLA Protein

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1173-1183页

作者： Di Wang Hao Gao Jian Wang Baoshan Cheng Gang Li Weijun Xu Xi Liu Huaiyin Institute of Agricultural Sciences in Xuhuai region of Jiangsu Huai’an223001China School of Life Sciences Huaiyin Normal UniversityHuai’an223300China key laboratory of eco-Agricultural biotechnology around hongze lake Regional Cooperative Innovation Center for Modern Agriculture and Environmental ProtectionHuaiyin Normal UniversityHuai’an223300China

Although tocopherols are essential for rice development,the molecular details by which their absence affects development remain to be *** study how tocopherols function during rice development,we performed a transcriptome deep sequencing(RNA-seq)analysis of the rice cultivar Nipponbare(Nip)and the tocopherol-deficient mutant small grain and dwarf 1-2(sgd1-2).We identified 563 differentially expressed genes that were enriched in Gene Ontology categories associated with metabolism,stress,cellular responses,and transcriptional *** determined that the total fatty acid composition of Nip and sgd1-2 was comparable,although cell membrane penetrability in sgd1-2 was significantly higher than in Nip under optimal growth conditions,indicating that tocopherol deficiency induces cell membrane *** expression levels of dehydration-responsive element binding 1(DREB1)genes and free proline content in sgd1-2 were also higher than those in *** also showed that the DELLA protein SLENDER RICE1(SLR1)accumulated in sgd1-2,resulting in significant changes in the global *** study confirms that the lack of tocopherol accumulation in rice induced ectopic stress responses and limited growth by enhancing SLR1 abundance through increasing SLR1 transcript *** results provide new insights into tocopherol during rice development.

关键词： Tocopherol deficiency ectopic stress response gibberellin rice

Increased BnaMFT-transcript level is associated with secondary dormancy in oilseed rape (Brassica napus L.)

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Journal of Integrative agriculture 2020年第6期19卷 1565-1576页

作者： LIU Lei FAN Wen-qi LIU Fu-xia YI Xin TANG Tang ZHOU Ying TANG Zi-wei CHEN Guimin ZHAO Xiang-xiang Jiangsu key laboratory for eco-agriculture biotechnology Around hongze lake/Jiangsu Collaborative Innovation Center of regional Modern agriculture and Environment Protection/College of Life Science Huaiyin Normal UniversityHuai'an 223300P.R.China School of Food Science and Engineering Yangzhou UniversityYangzhou 225127P.R.China

Brassica napus cultivars have little or no primary dormancy;however,they are prone to sec on dary dorma ncy in ducti *** dormant seeds can produce volunteer plants,which can result in genetic contamination,reduced quality and biosafety ***,informati on regarding the molecular mecha nism un derlyi ng sec on dary dormancy is *** MOTHER OF FT AND TFL1(MFT)gene,which is evolutionarily conserved in the plant kingdom,acts in a complex gene network in the seed dormancy or germination *** this study,we identified four *** genes that share high homology with AtMFT,named as *** of c/s-acting elements showed that BnaMFT promoters contain multiple seed-specific regulatory elements,and various stress-and hormone-responsive *** experiments validated that BnaMFTs were specifically expressed during seed maturation and in the dry seed,with peaks at 35-42 days after *** were not sufficient for primary dormancy;however,they were significantly enhanced by secondary dormancy induction with PEG6000 ***,BnaMFTtranscripts were elevated by treatment with abscisic acid(ABA),which is known to be accumulated duri ng sec on dary dorma *** results collectively suggest that increased BnaMFT tra nscription levels are associated with sec on dary dorma ncy inducti on in an ABA-depe ndent mariner in ***.

关键词： Brassica napus DELAY OF GERMINATION 1(DOG7) MFT primary dormancy secondary dormancy

Are humic substances soil microbial residues or unique synthesized compounds?A perspective on their distinctiveness

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Pedosphere 2020年第2期30卷 159-167页

作者： Sen DOU Jun SHAN Xiangyun SONG Rui CAO Meng WU Chenglin LI Song GUAN College of Resources and Environmental Sciences Jilin Agricultural UniversityChangchun 130118China State key laboratory of Soil and Sustainable agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China College of Resources and Environment Qingdao Agricultural UniversityQingdao 266109China Jiangsu key laboratory for eco-Agricultural biotechnology around hongze lake Huaiyin Normal UniversityHuaian 223300China

Humic substances(HS),which are defined as a series of highly acidic,relatively high-molecular-weight,and yellow to black colored substances formed during the decay and transformation of plant and microbial remains,ubiquitously occur in *** substances represent the largest stable organic carbon pool in terrestrial environments and are the central characteristic of the ***,the validity of the HS concept and the justification of their extraction procedure have been recently ***,we argue that the traditional humic paradigm is still *** substances are distinctive and complex because the extracted HS formed during the humification are chemically distinct from their precursors and are heterogeneous among *** reviewing the concept,formation pathways,and stabilization of HS,we propose that the key question facing soil scientists is whether HS are soil microbial residues or unique synthesized *** revealing the distinctiveness of HS,it is impossible to address this question,as the structure,composition,and reactivity of HS are still poorly known owing to the heterogeneity and geographical variability of HS and the limits of the currently available analytical *** our view,the distinctiveness of HS is fundamental to the soil,and thus further studies should be focused on revealing the distinctiveness of HS and explaining why HS hold this distinctiveness.

关键词： heterogeneity humic acid humification humus microbial biomass soil organic matter

Carbon Monoxide Modulates Auxin Transport and Nitric Oxide Signaling in Plants under Iron Deficiency Stress

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Phyton-International Journal of Experimental Botany 2024年第1期93卷 45-61页

作者： Kaiyue Hong Yasmina Radani Waqas Ahmad Ping Li Yuming Luo Jiangsu key laboratory for eco-Agricultural biotechnology around hongze lake Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental ProtectionHuaiyin Normal UniversityHuaian223300China School of Life Sciences Nanjing Forestry UniversityNanjing210037China School of Life Sciences Shanghai UniversityShanghai200444China

Carbon monoxide(CO)and nitric oxide(NO)are signal molecules that enhance plant adaptation to environmental *** is an essential phytohormone for plant growth and *** and NO play crucial roles in modulating the plant’s response to iron *** deficiency leads to an increase in the activity of heme oxygenase(HO)and the subsequent generation of ***,it alters the polar subcellular distribution of Pin-Formed 1(PIN1)proteins,resulting in enhanced auxin *** alteration,in turn,leads to an increase in NO ***,iron deficiency enhances the activity of ferric chelate reductase(FCR),as well as the expression of the Fer-like iron deficiency-induced transcription factor 1(FIT)and the ferric reduction oxidase 2(FRO2)genes in plant *** of the long hypocotyl 1(HY1)gene,which encodes heme oxygenase,or the CO donor treatment resulted in enhanced basipetal auxin transport,higher FCR activity,and the expression of FIT and FRO2 genes under Fe ***,a potential mechanism is proposed:CO and NO interact with auxin to address iron deficiency *** alters auxin transport,enhancing its accumulation in roots and up-regulating key iron-related genes like FRO2 and *** auxin levels affect NO signaling,leading to greater sensitivity in root *** interplay promotes FCR activity,which is crucial for iron ***,these molecules enhance iron uptake and root growth,revealing a novel aspect of plant physiology in adapting to environmental stress.

关键词： Carbon monoxide nitric oxide auxin iron deficiency signal molecule plants