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Cobalamin-dependent radical S-adenosyl-L-methionine protein functions with a partner to successively methylate tricyclic indole alkaloid for chuangxinmycin maturation and derivatization

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Science China Chemistry 2025年第1期68卷 308-316页

作者： Sili Wang Jiancheng Huang Yanan Du Wei Huang Yufeng Xue Xiaokun Xu Guannan Zhong Yuanyuan Shi Bin Hong Xiaoying Bian Wen Liu State Key laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200032China State Key laboratory of Microbial Technology Shandong UniversityQingdao 266237China National Health Commission(NHC)Key laboratory of biotechnology for Microbial Drugs Institute of Medicinal BiotechnologyChinese Academy of Medical Science&Peking Union Medical CollegeBeijing 100050China

Cobalamin(Cbl)-dependent radical S-adenosyl-L-methionine(SAM)proteins constitute the largest collection of the radical SAM superfamily that has hundreds of thousands of individual *** of these proteins are involved in the biosynthesis of pharmaceutically important natural products to catalyze chemically demanding *** the biosynthetic pathway of chuangxinmycin(CXM),a unique indole alkaloid antibiotic with potent anti-infective activity,functionalization of the characteristic thiopyrano[4,3,2-cd]indole scaffold by regio-and stereoselective C3-methylation is believed to rely on a Cbl-dependent radical process,which,however,remained to be reconstituted *** here report the dissection of this enzymatic process,which requires the incorporation of Cxm8,a Cbl-dependent radical SAM protein,with Cxm9,a DUF5825family protein that shares no homology to any proteins of known ***8 and Cxm9 function together by forming an unexpected heterodimeric complex that selectively catalyzes C3-methylation of the tricyclic indole-S-hetero ring system in a successive manner,achieving CXM and a recently identified,C3-dimethylated *** biochemical characterization,isotope labeling,structural simulation and bioinformatics analysis rationalized the catalysis of the Cxm8/Cxm9 complex and particularly the necessity of the DUF5825 protein for C3-methylase *** is the first example that a Cbl-dependent protein acts with a partner to exhibit radical SAM activity.

关键词： cbl-dependent radical SAM protein biosynthesis chuangxinmycin stereoselective methylation

Morphology and phylogeny of two species of Bathypathes from the tropical western Pacific,with description of a new species

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Journal of Oceanology and Limnology 2025年第1期43卷 248-260页

作者： Ting LÜ Zifeng ZHAN Kuidong XU laboratory of Marine Organism Taxonomy and Phylogeny Qingdao Key Laboratory of Marine Biodiversity and ConservationInstitute of OceanologyChinese Academy of SciencesQingdao 266071China laboratory for Marine Biology and biotechnology Laoshan LaboratoryQingdao 266237China University of Chinese Academy of Sciences Beijing 100049China

The black coral genus Bathypathes Brook,1889 represents a relatively diverse and ecologically important assemblage comprising the deep-sea hard substrate *** phylogenetic investigations have revealed the polyphyletic nature of Bathypathes,urging further morphological analysis to understand its *** combing morphological and molecular data,we describe Bathypathes multifurcata *** report *** Molodtsova,Opresko&Wagner,2022based on the specimens collected from the tropical western *** phylogenetic topology reconstructed using nuclear ITS1-5.8S-ITS2 and three mitochondrial fragments suggests the separation of Bathypathes into four *** analysis of morphological characters,particularly the colony branch and pinnule patterns also roughly classify Bathypathes into four morphological groups A–*** A is characterized by the unbranched colony and the subopposite arrangement of *** contrast to Group A,Groups B–D have the alternately arranged *** these,Group C exhibits a distinctly long unpinnulated stem compared to Groups B and *** B has unbranched colony,relatively short unpinnulated stem,and spines on pinnules have blunt *** Group D,large colonies(higher than 40 cm)have one or a few branches,small colonies(less than 40 cm)are unbranched,and spines on pinnules have acute *** data particularly those species without sequences are necessary to verify the classification of these groups.

关键词： Antipatharia black coral taxonomy phylogeny seamount western Pacific

Eukaryotic translation initiation factor OseIF5A4 negatively regulates rice immunity against blast and bacterial blight diseases

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Crop Journal 2025年第1期13卷 79-91页

作者： Wang, Hui Yan, Yuqing Bi, Yan Li, Dayong Song, Fengming National Key laboratory for Rice Biology and Breeding Institute of Biotechnology Zhejiang University Zhejiang Hangzhou 310058 China Ministry of Agriculture Key laboratory of Molecular Biology of Crop Pathogens and Insect Pests Institute of Biotechnology Zhejiang University Zhejiang Hangzhou 310058 China Key laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province Institute of Biotechnology Zhejiang University Zhejiang Hangzhou 310058 China

The RING-type E3 ligase OsBBI1 regulates rice resistance against Magnaporthe oryzae through modifying cell wall defenses. In this study, we report the function of an OsBBI1 substrate, eukaryotic translation initiation factor OseIF5A4, in rice immunity. OsBBI1 interacts with OseIF5A4 and other four members of the OseIF5A family. The RING domain in OsBBI1 and the eIF-5a domain in OseIF5A4 are critical for the OsBBI1-OseIF5A4 interaction. OsBBI1 ubiquitinates OseIF5A4 and mediates its degradation in vitro and in vivo. Moreover, the expression of OseIF5A4 was upregulated during early stage of compatible interaction but downregulated in incompatible interaction between rice and M. oryzae. Knockout of OseIF5A4 enhances rice immunity against M. oryzae and Xanthomonas oryzae pv. oryzae, boosts pattern-triggered immune responses, and strengthens pathogen-induced defense responses (e.g., expression of defense genes, accumulation of reactive oxygen species and reinforcement of cell wall). However, overexpression of OseIF5A4 attenuates rice immunity and immune responses. These results demonstrate that OseIF5A4, a substrate of the immunity-associated E3 ligase OsBBI1, negatively regulates rice immunity against M. oryzae and X. oryzae pv. oryzae through modulating pathogen-induced defense responses, highlighting the importance of the protein translational machinery in rice immunity. © 2025 Crop Science Society of China and Institute of Crop Science, CAAS

关键词： Magnaporthe oryzae OsBBI1 OseIF5A Rice immunity Xanthomonas oryzae pv. oryzae

Musashi orchestrates melanism in Laodelphax striatellus

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Insect Science 2025年第1期32卷 140-150页

作者： Zeping Mao Biyun Wang Youyuan Chen Jinjun Ying Haiqiang Wang Junmin Li Chuanxi Zhang Jichong Zhuo State Key laboratory for ManagingBiotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MARA and Institute of Plant VirologyNingbo UniversityNingboZhejiang Province315211China

In insects,melanism,a fundamental pigmentation process,is of significant importance in evolutionary biology due to its complex genetic *** investigated the role of the RNA-binding gene Musashi(msi)in melanism in Laodelphax striatellus,a Hemiptera *** identified a single *** msi homolog,Lsmsi,encoding a 357 amino acid protein with 2 RNA recognition *** interference-mediated knockdown of LsMsi resulted in complete body melanism and increased cuticular ***,we found the involvement of G protein-coupled receptor A42 and tyrosine hydroxylase(Th)in *** *** of LsTh lightened the epidermis,showing dehydration signs,while LsA42 knockdown enhanced LsTh expression,leading to ***,Lsmsi knockdown decreased both LsA42 and LsTh expression,which was expected to cause whitening but resulted in ***,we found that Lsmsi influenced downstream genes like phenoloxidase homolog LsPo and dopa decarboxylase(Ddc)homolog LsDdc in the tyrosine-mediated melanism *** to Nilaparvata lugens and Sogatella furcifera,we demonstrated the conserved role of msi in melanism among *** MSI proteins’roles in cancer and tumors in vertebrates,our study is the first to link msi in insects to Delphacidae body color melanization via the tyrosine-mediated pathway,offering fresh perspectives on the genetic basis of insect melanism and msi functions.

关键词： dopa decarboxylase melanism Musashi phenoloxidase planthopper

Isobaric enzymatic assays reveal depth-dependent variation in microbial ectoenzyme activities in the deepest ocean on Earth

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Science China Earth Sciences 2025年第1期68卷 185-194页

作者： Junwei CAO Jiahua WANG Hongge ZHANG Junhao DENG Binbin PAN Jiawang CHEN Li ZHANG Jiasong FANG College of Oceanography and Ecological Science Shanghai Ocean UniversityShanghai201306China Ocean College Zhejiang UniversityZhoushan316000China laboratory for Marine Biology and biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266237China

Microbial ectoenzymes play essential roles in the transformation and mineralization of organic matter in the ***,conventional methods for measuring microbial ectoenzyme activities(MEAs)in the deep ocean under atmospheric conditions can misrepresent the metabolic activities of indigenous microbial *** overcome this limitation,we measured the activities of three microbial ectoenzymes(aminopeptidase,α-glucosidase,andβ-glucosidase)under both atmospheric and in situ pressure conditions in seawater collected using pressure-retaining and non-pressure-retaining samplers in the Challenger Deep,Mariana *** the case of aminopeptidase andα-glucosidase,the highest isobaric MEAs were observed in the surface layer(50 m),followed by those at abyssopelagic depths(4,000–6,000 m)for aminopeptidase,hadal realm(10,903 m)forα-glucosidase,whileβ-glucosidase activity exhibited the highest value at 10,903 ***,the isobaric MEAs in hadal waters were commonly found to be higher than the decompressed counterparts,highlighting the importance of pressure-retaining sampling and isobaric enzymatic *** half-saturation constant(K_(m))showed a general decreasing trend with depth,suggesting that the deep ocean microbes might have adapted to the high-pressure and oligotrophic environment by increasing their ectoenzyme's affinity to ***,particle-free MEAs contribute more to the total MEAs in the deep ocean than particle-associated MEAs,suggesting the significance of cell-associated and dissolved ectoenzymes in ocean *** study provides a foundation for future investigations of MEAs in the ocean and has important implications for understanding the dynamics of microbially mediated biogeochemical cycling in marine ecosystems.

关键词： Pressure-retaining sampling Microbial ectoenzyme activity Deep-sea microbes High pressure Mariana Trench

A C-type lectin with dual carbohydrate recognition domains functions in innate immune response in Asian corn borer,Ostrinia furnacalis

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Insect Science 2025年第1期32卷 172-192页

作者： Er-Tao Li Jia-Yue Ji Wei-Jie Kong Dong-Xu Shen Cai Li Chun-Ju An Deparment of Entomology College of Plant ProtectionChina Agricultural UniversityBejingChina Pomology Institute Shanxi Agricultural UniversityJinzhongShanxi ProvinceChina Jiangsu Key laboratory of Sericultural Biology and biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina

C-type lectins(CTLs)act as pattern recognition receptors(PRRs)to initiate the innate immune response in insects.A CTL with dual carbohydrate recognition domains(CRDs)(named immulectin-4[IML-4])was selected from the Ostrinia furnacalis transcriptome dataset for functional *** cloned the full-length complementary DNA of *** IML-4(OfIML-4).It encodes a 328-residue protein with a Glu-Pro-Asn(EPN)and Gln-Pro-Asp(QPD)motifs in 2 CRDs,***-4 messenger RNA levels increased significantly upon the bacterial and fungal *** OfIML-4(rIML-4)and its individual CRDs(rCRD1 and rCRD2)exhibited the binding ability to various microorganisms including Escherichia coli,Micrococcus luteus,Pichia pastoris,and Beauveria bassiana,and the cell wall components including lipopolysaccharide from ***,peptidoglycan from *** or Bacillus subtilis,and curdlan from Alcaligenes *** binding further induced the agglutination of ***,***,and *** in the presence of calcium,the phagocytosis of Staphylococcus aureus by the hemocytes,in vitro encapsulation and melanization of nickel-nitrilotriacetic acid beads,and a significant increase in phenoloxidase activity of *** addition,rIML-4 significantly enhanced the phagocytosis,nodulation,and resistance of *** to *** together,our results suggest that OfIML-4 potentially works as a PRR to recognize the invading microorganisms,and functions in the innate immune response in ***.

关键词： cellular immunity C-type lectin humoral immunity immulectin Ostrinia furnacalis pattern recognition receptor(PRR)

The miR3367–lncRNA67–GhCYP724B module regulates male sterility by modulating brassinosteroid biosynthesis and interacting with Aorf27 in Gossypium hirsutum

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Journal of Integrative Plant Biology 2025年第1期67卷 169-190页

作者： Anhui Guo Hushuai Nie Huijing Li Bin Li Cheng Cheng Kaiyun Jiang Shengwei Zhu Nan Zhao Jinping Hua laboratory of Cotton Genetics Genomics and Breeding/Joint Laboratory for International Cooperation in Crop Molecular BreedingMinistry of Education/Beijing Key Laboratory of Crop Genetic ImprovementCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Key laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China

Cytoplasmic male sterile(CMS)lines play a crucial role in utilization of heterosis in crop ***,the mechanism underlying the manipulation of male sterility in cotton by long non-coding RNA(lncRNA)and brassinosteroids(BRs)remains ***,using an integrative approach combining lncRNA transcriptomic profiles with virus-induced gene silencing experiments,we identify a flower budspecific lncRNA in the maintainer line 2074B,lncRNA67,negatively modulating with male sterility in upland cotton(Gossypium hirsutum).lncRNA67positively regulates cytochrome P274B(GhCYP724B),which acted as an eTM(endogenous target mimic)for *** suppression of GhCYP724B induced symptoms of BR deficiency and male semisterility in upland cotton as well as in tobacco,which resulted from a reduction in the endogenous BR ***724B regulates BRs synthesis by interacting with GhDIM and GhCYP90B,two BRs biosynthesis ***,GhCYP724B suppressed a unique chimeric open reading frame(Aorf27)in 2074A mitochondrial *** expression of Aorf27 in yeast inhibited cellular growth,and over expression of Aorf27 in tobacco showed male ***,the results proved that the miR3367–lncRNA67–GhCYP724B module positively regulates male sterility by modulating BRs *** findings uncovered the function of lncRNA67–GhCYP724B in male sterility,providing a new mechanism for understanding male sterility in upland cotton.

关键词： brassinosteroids GhCYP724B lncRNA67 male sterility mitochondrial Aorf27 upland cotton(G.hirsutum)

Nanoplastics and nano-ZnO facilitate Cd accumulation in zebrafish larvae via a distinct pathway: Revelation by LA-ICP-MS imaging

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Chinese Chemical Letters 2025年第2期36卷 436-440页

作者： Pengyu Chen Beibei Chen Man He Yuxi Zhou Lei Lei Jian Han Bingsheng Zhou Ligang Hu Bin Hu Department of Chemistry Wuhan University State Key laboratory of Freshwater Ecology and biotechnology Institute of Hydrobiology Chinese Academy of Sciences State Key laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

The interaction between nanoparticles （NPs） and pollutants affects their bioavailability and ***,the processes by which NPs and pollutants change in vivo have rarely been ***,using laser ablation-inductively coupled plasma-mass spectrometry （LA-ICP–MS）,we found that both nanoplastics and ZnO NPs caused more Cd to accumulate in zebrafish larvae,but with distinct *** could adsorb Cd2+and transfer it into the larvae through the“Trojan horse”*** coexposure of nanoplastics and Cd2+caused Cd to accumulate in the abdomen where the nanoplastics were located without dissociation,showing a lower toxic effect than Cd2+exposure *** NPs weakly adsorbed Cd2+,but they increased the Zn and Cd contents in larvae by enhancing the expression of metal *** coexposure of ZnO and Cd2+evenly distributed Cd in the larvae,revealing a more severe toxic effect than Cd2+exposure *** results demonstrated the changing bioavailability and toxicity of Cd induced by different *** also shows the vital role LA-ICP-MS plays in revealing the relationship between toxicity and *** addition,the long-term effect of bioavailability on heavy metal toxicity and nanosafety deserves further investigation.

关键词： Cadmium Combined effect LA-ICP-MS Nanoplastics Zebrafish larvae ZnO nanoparticles

Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein

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Journal of Integrative Plant Biology 2025年第1期67卷 7-10页

作者： Wenbo Pan Chunlei Gao De Niu Jinghua Cheng Jiao Zhang Xiying Yan Qiang Long Yao Yao Zhu Wenjing Sun Qi Xie Yuehui He Xing Wang Deng Huawei Zhang Jian Li State Key laboratory of Wheat Improvement Shandong Laboratory of Advanced Agricultural Sciences in WeifangPeking University Institute of Advanced Agricultural SciencesWeifang 261325China Guangdong Provincial Key laboratory of biotechnology for Plant Development Ministry of Education&Guangdong Provincial Key Laboratory of Laser Life ScienceSchool of Life SciencesSouth China Normal UniversityGuangzhou 510631China Shenzhen Tidy field System biotechnology Co. LtdShenzhen 518107China State Key laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China

Despite significant advancements in the efficiency of the CRISPR/Cas9 genome editing system over the past decade,performing gene disruption in polyploid crops such as common wheat(Triticum aestivum L.)remains challeng... 详细信息

关键词： CRISPR/Cas9 crops trait