检索结果-南通市图书馆

Cellular reprogramming in farm animals： an overview of iPSC generation in the mammalian farm animal species

Journal of Animal Science and Biotechnology 2016年第4期7卷 459-467页

作者： J. Ogorevc S. Orehek P. DovcC Department of Animal Science Biotechnical Faculty University of LjubljanaDomzale Slovenia

Establishment of embryonic stem cell （ESC） lines has been successful in mouse and human, but not in farm animals. Development of direct reprogramming technology offers an alternative approach for generation of pluripotent stem cells, applicable also in farm animals. Induced pluripotent stem cells （iPSCs） represent practically limitless, ethically acceptable, individuum-specific source of pluripotent cells that can be generated from different types of somatic cells, iPSCs can differentiate to all cell types of an organism＇s body and have a tremendous potential for numerous applications in medicine, agriculture, and biotechnology. However, molecular mechanisms behind the reprogramming process remain largely unknown and hamper generation of bona fide iPSCs and their use in human clinical practice. Large animal models are essential to expand the knowledge obtained on rodents and facilitate development and validation of transplantation therapies in preclinical studies. Additionally, transgenic animals with special traits could be generated from genetically modified pluripotent cells, using advanced reproduction techniques. Despite their applicative potential, it seems that iPSCs in farm animals haven＇t received the deserved attention. The aim of this review was to provide a systematic overview on iPSC generation in the most important mammalian farm animal species （cattle, pig, horse, sheep, goat, and rabbit）, compare protein sequence similarity of pluripotency-related transcription factors in different species, and discuss potential uses of farm animal iPSCs. Literature mining revealed 32 studies, describing iPSC generation in pig （13 studies）, cattle （5） horse （5）, sheep （4）, goat （3）, and rabbit （2） that are summarized in a concise, tabular format.

关键词： Cellular reprogramming Farm animals Induced pluripotent stem cells Pluripotency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Effect of Printing Parameters and Wood Surface Preparation on the Adhesion of Directly 3D-Printed PLA on Wood

引用

Journal of Renewable Materials 2022年第7期10卷 1787-1796页

作者： Daša KrapežTomec Angela Balzano JureŽigon MilanŠernek Mirko Kariž Department of Wood Science and Technology Biotechnical FacultyUniversity of LjubljanaLjubljana1000Slovenia

As additive manufacturing technologies advance,new opportunities are opening up for their application in the furniture *** remains one of the leading raw materials in the furniture industry;therefore,possible options for combining it with 3D printing have been *** bonding of 3D-printed polymer parts with wood or 3D printing with wood-plastic composites is already known,but in our research we attempted to directly 3D print polylactic acid(PLA)on wood *** effect of printing parameters,as well as the surface preparation of wood on the shear strength of the bond between wood and on-printed material was *** images of cross-sections of samples were *** results show that with a lower initial layer thickness(0.1 mm),a higher printing temperature(220℃),and with the use of polyvinyl acetate(PVAc)primer on the wood surface before 3D printing,a higher bond strength(5.4 MPa)was achieved,but the values for the bond strength remain low compared to the conventional bonding of wood to wood with PVAc adhesive(around 10 MPa).Microscopy studies revealed barely visible penetration of PLA into the lumens of the wood ***,the PVAc adhesive used as primer penetrated more into the cell lumens and served as interface layer between deposited melted PLA and the wood,thus creating stronger joints.

关键词： FDM direct 3D printing adhesion printing parameters wood PLA PVAc

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Candidate genes for mastitis resistance in dairy cattle:a data integration approach

引用

Journal of Animal Science and Biotechnology 2023年第3期14卷 966-979页

作者： Zala Brajnik Jernej Ogorevc biotechnical faculty Department of Animal ScienceUniversity of LjubljanaGroblje 3Domzale SI‑1230Slovenia

Background Inflammation of the mammary tissue(mastitis)is one of the most detrimental health conditions in dairy ruminants and is considered the most economically important infectious disease of the dairy *** mastitis resistance is becoming an important goal in dairy ruminant breeding ***,mastitis resistance is a complex trait and identification of mastitis-associated alleles in livestock is ***,the only applicable approach to identify candidate loci for complex traits in large farm animals is to combine different information that supports the functionality of the identified genomic regions with respect to a complex *** To identify the most promising candidate loci for mastitis resistance we integrated heterogeneous data from multiple sources and compiled the information into a comprehensive database of mastitis-associated candidate ***-associated candidate genes reported in association,expression,and mouse model studies were collected by searching the relevant literature and *** collected data were integrated into a single database,screened for overlaps,and used for gene set enrichment *** The database contains candidate genes from association and expression studies and relevant transgenic mouse *** 2448 collected candidate loci are evenly distributed across bovine *** integration and analysis revealed overlaps between different studies and/or with mastitis-associated QTL,revealing promising candidate genes for mastitis *** Mastitis resistance is a complex trait influenced by numerous *** on the number of independent studies,we were able to prioritise candidate genes and propose a list of the 22 most *** our knowledge this is the most comprehensive database of mastitis associated candidate genes and could be helpful in selecting genes for functional validation studies.

关键词： Association study Candidate genes Epigenetics Mammary gland Mastitis Quantitative trait loci

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Polymorphisms in microRNA targets： a source of new molecular markers for male reproduction

引用

Asian Journal of Andrology 2011年第3期13卷 505-508页

作者： Jernej Ogorevc Peter Dovc Tanja Kunej Department of Animal Science Biotechnical Faculty University of Liubljana Domzale 1230 Slovenia

Dear Editor, Herein we discuss the impact of microRNA （miRNA） target genetic variability in male infertility genes, which can represent a source of novel molecular-genetic markers that can be used for the diagnosis of male infertility. Male-factor infertility accounts for 30%--40% of infertility cases. The causes of spermatogenetic failure found in most cases of non-obstructive azoospermia or severe oligozoospermia still remain idiopathic.

关键词： Physical sciences Condensed matter

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Leaf spectral signatures differ in plant species colonizing habitats along a hydrological gradient

引用

Journal of Plant Ecology 2016年第4期9卷 442-450页

作者： Katja Klančnik Alenka Gaberščik biotechnical faculty Department of BiologyUniversity of LjubljanaJamnikarjeva 101 SI-1000LjubljanaSlovenia

Aims We aimed at determining differences in the leaf spectral signatures of plant species groups growing in habitats along the hydrological gradient of an intermittent wetland and to define leaf traits that explain their *** want to contribute to the understanding of the causes for plant spectrum variability at leaf and community *** We measured leaf reflectance spectra(300-887 nm)of representative plant species from different habitats and analyzed spectral differences among species *** explain leaf spectra variability within a group,we performed detailed analyses of leaf morphological and biochemical traits in selected *** FindingsThe reflectance spectra of the different species groups differed most in the green,yellow and red spectral *** reflectance spectra of submerged leaves of hydrophytes with simple structures were explained by their biochemical traits(carotenoids),while for more complex aerial leaves,morphological traits were more *** submerged and natant leaves of amphiphytes,total mesophyll and spongy tissue thickness were the most important traits,and these explained 44%and 47%,respectively,of the spectrum variability of each plant *** general,the redundancy analysis biplots show that samples of different plant species colonizing the same habitat form separate clusters and are related to the explanatory variables in different *** redundancy analysis biplots of helophytes and wet meadow species show clustering of graminoids and dicots into two distinct *** encrustation(prickle hair properties and epidermis thickness)is important for graminoids,while leaf thickness and specific leaf area have more important roles in *** results show that knowledge of the species composition and leaf traits is necessary to interpret the reflectance spectra of such plant communities.

关键词： leaf traits habitats hydrological gradient reflectance spectra wetland

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Curing Kinetics of Tannin and Lignin Biobased Adhesives Determined by DSC and ABES

引用

Journal of Renewable Materials 2022年第8期10卷 2117-2131页

作者： Jaša Saražin Ida Poljanšek Antonio Pizzi MilanŠernek University of Ljubljana Biotechnical FacultyLjubljana1000Slovenia Universitéde Lorraine LERMAB-ENSTIB Epinal88000France

The curing process of two biobased adhesives:pine tanninhexamine(TH)and organosolv lignin non-isocyanate polyurethane(NIPU),suitable for interior nonstructural use,were compared with commercial urea-formaldehyde(UF)*** in chemical structure before and after the curing process were observed with Fouriertransform infrared spectroscopy(FTIR).The process of adhesive curing was monitored with differential scanning calorimetry(DSC)and the automated bonding evaluation system(ABES).Both DSC and ABES measurements confirmed UF as the fastest and NIPU as the slowest curing adhesive *** into account the ABES results,the optimal pressing parameters for the TH adhesive would be 4 min at 175℃,for the NIPU adhesive 7 min at 200℃and for the UF 1.5 min at 100℃.Strong linear correlation was observed between mechanical and chemical curing for the UF and NIPU adhesives,whereas lower correlation was observed for the TH *** all observed adhesives,the DSC measurements were underestimating the curing process determined by ABES in the first part and overestimating it at the *** underestimation was the most evident with the TH adhesive and the less with the UF *** comparing the uncured and cured FTIR spectra of all three types of adhesives,a drastic decrease in the characteristic band of-OH groups at 3330–3400 cm^(−1)and an increase in the signal intensity at 2920 cm^(−1)of aliphatic-CH2-groups were *** the UF adhesive,the C=O stretching frequency has shifted from 1632 cm^(−1)for uncured to three different bands at 1766,1701,and 1655 cm^(−1)for cured *** sharp band for phenolic alcohols at 1236 cm^(−1)of C–O stretch and hydroxyl O–H functional group at 1009 cm^(−1)and at 684 cm^(−1)of uncured TH adhesive diminished during curing,which indicates that a crosslinking reaction occurs via-OH *** peak of the C=O group of urethane bridges at 1697 cm^(−1)for uncured NIPU shifted to lower wavenumber at 1633 cm^(−1)for cured NIPU.

关键词： Biobased adhesives curing kinetics tannin lignin ABES DSC FTIR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Characterization of Curing and Bonding of Wood with Adhesive Mixtures of Liquefied Wood and Hexamethylenediamine

引用

Journal of Renewable Materials 2023年第2期11卷 991-1001页

作者： JureŽigon VitŠeda PetrČermák MilanŠernek Department of Wood Science and Technology Biotechnical FacultyUniversity of LjubljanaLjubljana1000Slovenia Department of Wood Science and Technology Faculty of Forestry and Wood TechnologyMendel University in BrnoBrno61300Czech Republic

Various crosslinking agents can be added to the formulations of natural-based adhesives for wood bonding in order to achieve better durability and higher strength of the formed *** the present study,the effect of hexamethylenediamine(HMDA)addition on the performance of liquefied wood(LW)adhesive for wood bonding is *** scanning calorimetry showed the improved thermal stability and crosslinking of the LW adhesive with *** intensified presence of amide linkages(C–N bonds)was found in LW+HMDA with attenuated total reflection Fourier transform infrared *** of the bonded joints using an automated bonding evaluation system showed that a higher press temperature resulted in stronger bonds for both types of ***,the addition of HMDA to LW adhesive improved the bond strength of the joints and accelerated the crosslinking of the ***,with a tensile shear strength of(6.76±2.16)N×mm^(−2)(for LW)and(6.89±2.10)N×mm^(−2)(for LW+HMDA),both adhesives were found to be unsuitable for interior non-structural *** addition,the acidity of LW resulted in relatively high wood failure(70%)in the adhesive joints *** crosslinking of LW with HMDA was reflected in improved resistance of LW+HMDA adhesive joints to water *** conclusion,HMDA is a promising additive for improving the adhesive performance of LW adhesives.

关键词： Adhesive bonding hexamethylenediamine liquefied wood

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Organosolv Lignin for Non-Isocyanate Based Polyurethanes (NIPU) as Wood Adhesive

引用

Journal of Renewable Materials 2021年第5期9卷 881-907页

作者： Jaša Saražin Antonio Pizzi Siham Amirou Detlef Schmiedl MilanŠernek biotechnical faculty University of LjubljanaLjubljana1000Slovenia LERMAB-ENSTIB Universitéde LorraineEpinal88000France Fraunhofer Institute for Chemical Technology Pfinztal76327Germany

A non-isocyanate-based polyurethane(NIPU)wood adhesive was produced from organosolv lignin,which is a bio-sourced raw material,available in large quantities and produced as a by-product of the paper *** formulation of this new lignin-based NIPU adhesive,which is presented,was chemically characterised by Matrix-Assisted Laser Desorption Ionization Time of Flight(MALDI ToF)mass spectrometry and by Fourier Transform Infra-Red(FTIR)spectrometry *** oligomers formed were determined and showed that the three species involved in the NIPU adhesive preparation were formed by the co-reaction of the three reagents used:lignin,dimethyl carbonate,and hexamethylene *** and branched structures were both *** properties of the adhesive were determined using the Automated Bonding Evaluation System(ABES)and internal bond(IB)strength test of the laboratory particleboard bonded with *** adhesive has shown satisfactory mechanical properties after hot pressing at 230℃.Such a temperature is used industrially in the most modern particleboard factories,but since it is hardly feasible for more conventional wood bonding equipment,the reactivity of the NIPU adhesive was successfully increased with the addition of a small percentage of a silane coupling *** the addition of the silane,the proposed NIPU adhesive could also be used at a hot-pressing temperature lower than 200℃.

关键词： Organosolv lignin NIPU MALDI ToF FTIR ABES

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Comparative Study on the Role of Cytokinins in Caryopsis Development in the Maize miniature1 Seed Mutant and Its Wild Type

引用

Journal of Integrative Plant Biology 2009年第9期51卷 840-849页

作者： Tomaz Rijavec Maja Kova Ale Kladnik Prem S.Chourey Marina Dermastia National Institute of Biology Department of Biotechnology and Systems Biology Department of Biology Biotechnical FacultyUniversity of Ljubljana US Department of Agriculture Agricultural Research ServiceChemistry Research Unit

We report here on a comparative developmental profile of plant hormone cytokinins in relation to cell size, cell number and endoreduplicaUon in developing maize caryopsis of a cell wall invertase-deficient miniature1 （mn1） seed mutant and its wild type, Mn1, genotype. Both genotypes showed extremely high levels of total cytokinins during the very early stages of development, followed by a marked and genotype specific reduction. While the decrease of cytokinins in Mn1 was associated with their deactivation by 9-glucosylation, the absolute and the relative part of active cytokinin forms was higher in the mutant. During the exponential growth phase of endosperm between 6 d after pollination and 9 d after pollination, the mean cell doubling time, the absolute growth rate and the level of endoreduplication were similar in the two genotypes. However, the entire duration of growth was longer in Mnl compared with mnl, resulting in a significantly higher cell number in the Mnl endosperm. These data correlate with the previously reported peak levels of the Mn1-encoded cell wall invertase-2 （INCW2） at 12 d after pollination in the Mn1 endosperm. A model showing possible crosstalk among cytokinins, cell cycle and cell wall invertase as causal to increased cell number and sink strength of the Mn1 developing endosperm is discussed.

关键词： 9-glucosylation cell wall invertase cytokinins maize caryopsis miniature1.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plotting the Clarifying Curves and Determination of the Specific Amount of Settled Material during the Initial Period to Sedimentation in Stationary Column of Aqueous Suspensions of Solids

引用

Journal of Mechanics Engineering and Automation 2013年第4期3卷 203-208页

作者： Victor Viorel Safta Mirela Dilea Gabriel Alexandru Constantin Department of biotechnical Systems Faculty of Biotechnical Systems Engineering Politehnica University of Bucharest Bucharest060042 Romania

The present paper presents an algorithm and a program for plotting the clarifying curves and for determination of the specific amount of settled material during the initial period based on data obtained from sedimentation experiments in stationary column of aqueous suspensions of solids particles with different concentrations. The algorithm and program have been developed for an interactive, rapid and convenient processing of the data obtained from sedimentation experiments in stationary column of aqueous suspensions of solids particles and allow obtaining with accuracy of precise and expressive graphs which characterize the behaviour of aqueous suspensions to sedimentation in stationary column. Sedimentation study of different aqueous suspensions of solids particles in stationary column is of great importance for experimentally determining the important parameters required to design and exploitation clarifiers and sludge thickeners from waste water treatment plants.

关键词： Sedimentation in stationary column of aqueous suspensions algorithm program clarifying curves specific amount ofsettled material during the initial period

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：