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A novel human coronavirus:Middle East respiratory syndrome human coronavirus

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Science China(Life Sciences) 2013年第8期56卷 683-687页

作者： GENG HeYuan TAN WenJie biotech center for viral disease emergency Key Laboratory of Medical Virology and Viral Diseases Prevention and ControlMinistry of HealthNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention

In 2012,a novel coronavirus,initially named as human coronavirus EMC(HCoV-EMC) but recently renamed as Middle East respiratory syndrome human coronavirus(MERS-CoV),was identified in patients who suffered severe acute respiratory infection and subsequent renal failure that resulted in *** epidemiological investigations together with retrospective studies have found 61 laboratory-confirmed cases of infection with this novel coronavirus,including 34 deaths to *** novel coronavirus is culturable and two complete genome sequences are now ***,molecular detection and indirect immunofluorescence assay have been *** present paper summarises the limited recent advances of this novel human coronavirus,including its discovery,genomic characterisation and detection.

关键词： HCoV-EMC MERS-CoV genomic characterisation molecular detection

Intranasal Immunization Using CTA1-DD as a Mucosal Adjuvant for an Inactivated Influenza Vaccine

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Biomedical and Environmental Sciences 2019年第7期32卷 531-540页

作者： FAN Xue Ting WANG Yun Long SU Qiu Dong QIU Feng YI Yao JIA Zhi Yuan WANG Da Yan QIN Kun ZOU Ye Ning BI Sheng Li SHEN Li Ping National Institute for viral disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Henan Bioengineering Research center Zhengzhou 4500466 Henan China Sinovac biotech Co. Ltd. Beijing 100085 China

Objective To evaluate the effect of intranasal immunization with CTA1-DD as mucosal adjuvant combined with H3N2 split vaccine. Methods Mice were immunized intranasally with PBS(negative control), or H3N2 split vaccine(3 μg/mouse) alone, or CTA1-DD(5 μg/mouse) alone, or H3N2 split vaccine(3 μg/mouse) plus CTA1-DD(5 μg/mouse). Positive control mice were immunized intramuscularly with H3N2 split vaccine(3 μg/mouse) and alum adjuvant. All the mice were immunized twice, two weeks apart. Then sera and mucosal lavages were collected. The specific HI titers, IgM, IgG, IgA, and IgG subtypes were examined by ELISA. IFN-γ and IL-4 were test by ELISpot. In addition, two weeks after the last immunization, surivival after H3N2 virus lethal challenge was measured. Results H3N2 split vaccine formulated with CTA1-DD could elicit higher Ig M, Ig G and hemagglutination inhibition titers in sera. Furthermore, using CTA1-DD as adjuvant significantly improved mucosal secretory Ig A titers in bronchoalveolar lavages and vaginal lavages. Meanwhile this mucosal adjuvant could enhance Th-1-type responses and induce protective hemagglutination inhibition titers. Notably, the addition of CTA1-DD to split vaccine provided 100% protection against lethal infection by the H3N2 virus. Conclusion CTA1-DD could promote mucosal, humoral and cell-mediated immune responses, which supports the further development of CTA1-DD as a mucosal adjuvant for mucosal vaccines.

关键词： Adjuvants H3N2 Influenza Mucosal immunization Split vaccine

Serological Survey of Zika Virus in Humans and Animals in Dejiang Prefecture, Guizhou Province,China

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Biomedical and Environmental Sciences 2019年第12期32卷 875-880页

作者： LI Fan ZHOU Jing Zhu ZHOU Lei FU Shi Hong TIAN Zhen Zao WANG Qi SHAO Nan LI Dan HE Ying LEI Wen Wen TANG Guang Peng LIANG Guo Dong WANG Ding Ming ZHANG Yan Ping WANG Huan Yu NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State Key Laboratory for Infectious disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China Guizhou center for disease Control and Prevention Guiyang 550004China Public Health emergency center Chinese Center for Disease Control and PreventionBeijing 102206China

Objective The current outbreak of Zika virus(ZIKV)poses a severe threat to human *** ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016,which was the first isolation of ZIKV in nature in *** In this study,serum samples were collected from 366 healthy individuals and 104 animals from Dejiang prefecture in 2017,and the plaque reduction neutralization test(PRNT)was used to evaluate the seroprevalence of *** None of the 366 residents from whom the samples were collected were seropositive for *** of the 11 pigs from whom the samples were collected were seropositive for ZIKV,while 1 of 63(1.59%)chickens and 2 of 30(6.67%)sheep were seropositive for *** The extremely low seropositivity rate of ZIKV antibodies in animals in the Dejiang prefecture,Guizhou province in this study indicates that ZIKV can infect animals;however,there is a low risk of ZIKV circulating in the local population.

关键词： Zika Virus Serological survey Humans Animals China Plague reduction neutralization test

Monkeypox virus:past and present

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World Journal of Pediatrics 2023年第3期19卷 224-230页

作者： Ya-Mei Dou Hang Yuan Hou-Wen Tian NHC Key Laboratory of Medical Virology and viral disease Chinese Center for Disease Control and PreventionNational Institute for Viral Disease Control and Prevention155 Changbai RoadChangPing DistrictBeijing 102206China

Background The objective of this paper is to analyze the current status of monkeypox *** the face of this public health threat,our purpose is to elucidate the clinical characteristics and epidemiology of monkeypox,the developmental progress of monkeypox-related drugs and the vaccines *** sources The literature review was performed in databases including PubMed,Science Direct and Google Scholar up to July *** Since May 2022,the World Health Organization has reported more than 45,000 confirmed cases from 92 nonendemic countries,including nine *** some women and children have been infected so far,most cases have occurred among men who have sex with other men,especially those with multiple sexual partners or anonymous *** Pediatric monkeypox infection has been associated with a higher likelihood of severe illness and mortality than in *** monkeypox illness in pediatrics often requires adjunctive antiviral *** is crucial for all countries to establish sound monitoring and testing systems and be prepared with emergency preparedness.

关键词： Monkeypox Orthopoxvirus Smallpox Zoonotic

Dr. Chi-Ming Chu： Respected founder of molecular virology and pioneer of biologicals in China

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Protein & Cell 2017年第9期8卷 629-633页

作者： Weizheng Yan Baoying Huang Li Ruan Wenjie Tan biotech center for viral diseases emergency Key Laboratory of Medical Virology Ministry of Health National Institute for ViralDisease Control and Prevention China CDC Beijing 102206 China

＂I have the greatest respect for Dr. Chu, who upheld great standards of science during very difficult times in China. His contributions to the genetic characterisation of influenza viruses were extraordinary.＂

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Papua New Guinea Under the COVID-19 Pandemic and Public Health Support from the World Health Organization

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China CDC weekly 2021年第50期3卷 1062-1064页

作者： Wenwen Lei National Institute for viral disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina

On March 20,2020,the first positive case of coronavirus disease 2019(COVID-19)was detected in Papua New Guinea(PNG).That same day,a state of emergency was declared in the country,thereby halting all incoming and domestic flights and limiting travel between ***,the PNG government established the National Control Centre(NCC)to specifically respond to the pandemic.

关键词： travel thereby limiting

Development and Assessment of Two Highly Pathogenic Avian Influenza(HPAI) H5N6 Candidate Vaccine Viruses for Pandemic Preparedness

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Biomedical and Environmental Sciences 2020年第9期33卷 670-679页

作者： LIU Li Qi LI Zi JIAO Ming LU Jian ZHOU Jian Fang LI Xi Yan LIU Jia GUO Jun Feng XIAO Ning ZHAO Xiang WANG Da Yan Chinese National Influenza center National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China

Objective In China, 24 cases of human infection with highly pathogenic avian influenza(HPAI) H5 N6 virus have been confirmed since the first confirmed case in 2014. Therefore, we developed and assessed two H5 N6 candidate vaccine viruses(CVVs).Methods In accordance with the World Health Organization(WHO) recommendations, we constructed two reassortant viruses using reverse genetics(RG) technology to match the two different epidemic H5 N6 viruses. We performed complete genome sequencing to determine the genetic stability. We assessed the growth ability of the studied viruses in MDCK cells and conducted a hemagglutination inhibition assay to analyze their antigenicity. Pathogenicity attenuation was also evaluated in vitro and in *** The results showed that no mutations occurred in hemagglutinin or neuraminidase, and both CVVs retained their original antigenicity. The replication capacity of the two CVVs reached a level similar to that of A/Puerto Rico/8/34 in MDCK cells. The two CVVs showed low pathogenicity in vitro and in vivo, which are in line with the WHO requirements for *** We obtained two genetically stable CVVs of HPAI H5N6 with high growth characteristics,which may aid in our preparedness for a potential H5N6 pandemic.

关键词： Highly pathogenic avian influenza H5N6 virus Genetic stability Candidate vaccine virus Reverse genetic technology

Molecular Evolution of Protein Sequences and Codon Usage in Monkeypox Viruses

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Genomics, Proteomics & Bioinformatics 2024年第1期22卷 89-97页

作者： Ke-Jia Shan Changcheng Wu Xiaolu Tang Roujian Lu Yaling Hu Wenjie Tan Jian Lu State Key Laboratory of Protein and Plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijing 100871China Sinovac biotech Ltd. Beijing 100085China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 100052China

The monkeypox virus(mpox virus,MPXV)epidemic in 2022 has posed a significant public health ***,the evolutionary principles of MPXV remain largely ***,we examined the evolutionary patterns of protein sequences and codon usage in *** first demonstrated the signal of positive selection in OPG027,specifically in the CladeⅠlineage of ***,we discovered accelerated protein sequence evolution over time in the variants responsible for the 2022 ***,we showed strong epistasis between amino acid substitutions located in different *** codon adaptation index(CAI)analysis revealed that MPXV genes tended to use more non-preferred codons compared to human genes,and the CAI decreased over time and diverged between clades,with CladeⅠ>Ⅱa andⅡb-A>Ⅱ*** the decrease in fatality rate among the three groups aligned with the CAI pattern,it remains unclear whether this correlation was coincidental or if the deoptimization of codon usage in MPXV led to a reduction in fatality *** study sheds new light on the mechanisms that govern the evolution of MPXV in human populations.

关键词： Mpox virus Positive selection OPG027 Accelerated evolution Codon usage bias

An Outbreak of Febrile Histoplasmosis Among Chinese Manganese-Mine Workers in Cooperative Republic of Guyana in 2019

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China CDC weekly 2019年第3期1卷 38-42页

作者： Lei Zhou Xin Wang Jiandong Li Rui Song Xiaoping Dong George F.Gao Zijian Feng Public Health emergency center Chinese Center for Disease Control and PreventionBeijingChina Department of Epidemiology and Biostatistics School of Public HealthPeking University Health Science CenterBeijingChina National Institute for Communicable disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Institute for viral disease Control and Prevention Chinese Center for Disease Control and PreventionBeijingChina Beijing Ditan Hospital Capital Medical UniversityBeijingChina Global Public Health center Chinese Center for Disease Control and PreventionBeijingChina State Key Laboratory for Infectious disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases(Zhejiang University)National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Chinese center for disease Control and Prevention BeijingChina

Summary What is already known on this topic?Cases of histoplasmosis have been reported in every continent except Antarctica but are fairly rare in *** prevalence of histoplasmosis has been observed in Central America,Caribbean,and South *** of Histoplasma are acquired through a respiratory route,particularly inhalation of aerosols from disturbed soils enriched with excreta from birds and bats.

关键词： China soils disturbed