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Down-regulation of microRNA-23a promotes pancreatic ductal adenocarcinoma initiation and progression by up-regulation of FOXM1 expression

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Genes & Diseases 2024年第5期11卷 456-469页

作者： Lixin Liang Tian Cai Xiaojia Li Jianhong An Sen Yu Yang Zhang Fengjie Guo Fang Wei Jie He Keping Xie Tingting Jiang center for Pancreatic Cancer Research South China University of Technology School of MedicineGuangzhouGuangdong 510006China Department of Laboratory Medicine The Sixth Affiliated Hospital and Nanhai People’s HospitalSouth China University of Technology School of MedicineFoshanGuangdong 528200China The Second Affiliated Hospital and Guangzhou First People’s Hospital South China University of Technology School of MedicineGuangzhouGuangdong 510006China

Transcriptional factor Forkhead box M1(FOXM1)plays an important role in pancreatic ductal adenocarcinoma(PDAC)development and *** molecular mechanisms underlying its dysregulation remain *** identified and functionally validated the microRNAs(miRNAs)that critically regulate FOXM1 expression in *** expression levels of miRNA-23a(miR-23a-3p and-5p)were altered in PDAC cell lines and their effects on FOXM1 signaling and cell proliferation and migration and tumorigenesis were examined in vitro and in vivo using mouse PDAC *** with non-tumor pancreatic tissues,PDAC tissues and cell lines exhibited significantly reduced levels of miR-23a *** miR-23a expression and concomitant increase in FOXM1 expression were also observed in acinar-toductal metaplasia and pancreatic intraepithelial neoplasia,the major premalignant lesions of *** expression of miR-23a reduced the expression of FOXM1 and suppressed cell proliferation and migration in PDAC cells,whereas the inhibitors of miR-23a did the *** or reduced levels of miR-23a increased the levels of FOXM1 expression,while increased expression of FOXM1 down-regulated miR-23a expression,suggesting that miR-23a and FOXM1 were mutual negative regulators of their expression in PDAC ***,the miR-23a/FOXM1 signaling axis is important in PDAC initiation and progression and could serve as an interventional or therapeutic target for patients with early or late stages of PDAC.

关键词： FOXM1 Gene expression Gene regulation MicroRNA Pancreatic cancer Targeted therapy

Quantifying and sorting of gold nanoparticle dimers from complex reaction mixtures using flow cytometry

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Nano Research 2016年第10期9卷 3093-3098页

作者： Jens B. Simonsen Nini E. A. Reeler Anna Fossum Knud A. Lerstrup Bo W. Laursen Kasper Nergaard Universitetsparken 5 Nano-Science Center & Department of Chemistry University of Copenhagen Copenhagen DK-2100 Denmark Niels Jensens Vej 2 Sino-Danish Centre for Education and Research (SDC) Aarhus DK-8000 Denmark Ole Maaloes Vej 5 Biotech Research and Innovation Centre (BRIC) University of Copenhagen Copenhagen DK-2200 Denmark

The formation, characterization, and purification of well-defined stoichiometric clusters of metallic nanoparticles, particularly in the form of dimers or trimers, are important and formidable challenges in nanoscience. Here we show that flow cytometry can be used as a high-throughput method to determine the relative distribution of oligomeric clusters of molecularly linked gold nano- particles in bulk solution at the single-particle level with good statistics. This unique information would be near impossible to obtain using traditional characterization techniques. The flow cytometric approach is utilized to provide fast feedback for the synthesis optimization of the complex reaction between citrate-stabilized gold nanoparticles and bi-functional molecular wires with dithiocarbamate anchoring groups. Finally, we demonstrate that flow cytometry can be used to significantly increase the proportion of AuNP dimers from an oligomer-rich polydisperse sample by size-selective sorting.

关键词： flow cytometry characterization sorting nanoparticles gold nanoparticle dimers

Prevention against diffuse spinal cord astrocytoma: can the Notch pathway be a novel treatment target?

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Neural Regeneration Research 2015年第2期10卷 244-251页

作者： Jian-jun Sun Zhen-yu Wang Ling-song Li Hai-yan Yu Yong-sheng Xu Hai-bo Wu Yi Luo Bin Liu Mei Zheng Jin-long Mao Xiao-hui Lou Department of Neurosurgery Peking University Third Hospital Peking University China Stem Cell Research center Peking University Health Science Center Clinical Stem Cell center Peking University Third Hospital Clinical Laboratory of Tissue & Cell Research center Department of Biotech Treatment Logistics College of Chinese People’s Armed Police Force Department of Neuroradiology Peking University Third Hospital Department of Neurology Peking University Third Hospital Neurosurgical Department Peking Union Medical College Hospital Department of Neurosurgery Rui’an People’s Hospital Wenzhou Medical University

This study was designed to investigate whether the Notch pathway is involved in the develop-ment of diffuse spinal cord astrocytomas. BALB/c nude mice received injections of CD133＋and CD133？ cell suspensions prepared using human recurrent diffuse spinal cord astrocytoma tissue through administration into the right parietal lobe. After 7–11 weeks, magnetic resonance imaging was performed weekly. Xenografts were observed on the surfaces of the brains of mice receiving the CD133＋ cell suspension, and Notch-immunopositive expression was observed in the xenografts. By contrast, no xenografts appeared in the identical position on the surfaces of the brains of mice receiving the CD133？ cell suspension, and Notch-immunopositive expres-sion was hardly detected either. Hematoxylin-eosin staining and immunohistochemical staining revealed xenografts on the convex surfaces of the brains of mice that underwent CD133＋ astro-cytoma transplantation. Some sporadic astroglioma cells showed pseudopodium-like structures, which extended into the cerebral white matter. However,it should be emphasized that the sub-cortex xenograft with Notch-immunopositive expression was found in the fourth mouse received injection of CD133？ astrocytoma cells. However, these ifndings suggest that the Notch pathway plays an important role in the formation of astrocytomas, and can be considered a novel treat-ment target for diffuse spinal cord astrocytoma.

关键词： nerve regeneration astrocytoma mice immunodeficiency (BALB/c) mice Notch nestin glial fibrillary acidic protein CD133 spinal cord brain MRI neural regeneration

A proximity-induced covalent fluorescent probe for selective detection of bromodomain 4

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Chinese Chemical Letters 2018年第7期29卷 1147-1150页

作者： Ling Feng Mohit Chhabra Wing Ho So Qing Zhu Jiang Xia Hongyan Sun Department of Chemistry and center of Super-Diamond and Advanced Films (COSDAF) City University of Hong Kong Key Laboratory of Biochip Technology Biotech and Health Centre Shenzhen Research Institute of City University of Hong Kong Department of Chemistry The Chinese University of Hong Kong College of biotechnology and bioengineering Zhejiang University of Technology

Lysine acetylation is one of the most prevalent and important posttranslational modifications(PTMs) in proteins. The process can be recognized by bromodomains(BRDs), which are a class of proteininteraction modules involved in chromatin remodeling and transcriptional activation. The development of BRD fluorescent probes will be useful for monitoring the activity of BRDs in living cells as well as aiding inhibitor development. Herein we designed a peptide-based probe based on the proximity-induced protein conjugation reaction. The peptide-based probe is capable of covalently and selectively reacting with the unique cysteine residue in the bromodomain through proximity effect. Our experimental data showed that the probe displayed noticeable fluorescence response upon addition of BRD4(1). In-gel fluorescence scanning demonstrated that BRD4(1) can be covalently labelled by the probe. Moreover, the probe was shown to selectively detect BRD4(1) over other proteins. We envision that the probe developed in this study will provide a useful tool to further investigate the biological roles of BRDs.

关键词： Peptide-based probe Bromodomain Fluorescent probe Proximity effect Peptides

Immunogenicity and Safety of Homologous Booster Doses of CoronaVac COVID-19 Vaccine in Elderly Individuals Aged 60 Years and Older:A Dosing Interval Study—Yunnan Province,China,2021–2022

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China CDC weekly 2023年第6期5卷 125-130,I0008-I0012页

作者： Haitao Yang Xing Meng Tingyu Zhuang Cangning Wang Zhongliang Yang Taotao Zhu Mei Li Yan Zheng Qianhui Wu Yaling Hu Hongjie Yu Xiaoqiang Liu Gang Zeng Yunnan Provincial center for Disease Control and Prevention Kunming CityYunnan ProvinceChina Sinovac biotech Co. Ltd.Beijing MunicipalityChina School of Public Health Fudan UniversityKey Laboratory of Public Health SafetyMinistry of EducationShanghai MunicipalityChina Yongde County center for Disease Control and Prevention Lincang CityYunnan ProvinceChina Sinovac Life Sciences Beijing MunicipalityChina

Summary What is already known about this topic?Neutralization levels induced by inactivated vaccines rapidly wane after primary immunization,and a homologous booster can recall specific immune memory,resulting in a re... 详细信息

关键词： concentration. doses remarkable

Parallel Detection of Bacterial Pathogens in Cerebrospinal Fluid with 16S rRNA Probe Microarray

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Journal of Microbiology and Immunology 2004年第3期2卷 210-217页

作者：刘毅韩金祥朱波黄海燕 Key Laboratory for biotech-Drugs Ministry of Health Shandong Medicinal Biotechnology Center Jinan 250062 China Medical School of Shandong University Jinan 250012 China Key Laboratory for biotech-Drugs Ministry of Health Shandong Medicinal Biotechnology Center Jinan 250062 China

To establish the microarray technique with 16S rRNA probe for the rapid detection of pathogenic bacteria in cerebrospinal fluid, a set of Digoxin labelling universal primers were designed, and used to amplify the 16S rRNA gene, and 2 specific probes for each bacterium designed were spotted onto nylon membrane to make a microarray, followed by hybridization to PCR products of bacteria, which set up a microarray technique for parallel detection of bacteria in cerebrospinal fluid (CSF). Twenty-nine CSF specimens from 16 patients that were proved to be positive by bacterial cultures and by using Vitek AMA-32 automated system were examined in the present investigation. It was found that positive fragment with length of 168?bp were amplified from 45 strains of 20 bacterial species, and amplifications also detected in 29 CSF specimens. The method of universal PCR could detect as few as 1 pg of bacterial DNA, and this microarray could identify all the bacterial species in CSF except Mycobacterium tuberculosis and Proteus mirabilis , satisfying the direct detection of bacteria in CSF specimens including a case of mixed bacterial infection. The positive and negative predictive value as well as the diagnostic index of this microarray were 100%, 94.67%, and 180.88 respectively, and the time of performance spent only for 5?h. It was concluded the method described in the present investigation can be used to detect and identify the pathogenic bacteria in CSF.

关键词： 16S rDNA Microarray Cerebrospinal fluid (CSF) Bacteria

Development of a target capture sequencing SNP genotyping platform for genetic analysis and genomic breeding in rapeseed

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The Crop Journal 2023年第2期11卷 499-510页

作者： Xiaodong Li Xumei Liu Yonghai Fan Shengting Li Mengna Yu Mingchao Qian Yuling Chen Hongqiao Chen Xinchun Li Bei Liu Xinfu Xu Cunmin Qu Jiana Li Kun Lu Integrative Science center of Germplasm Creation in Western China(CHONGQING)Science City and Southwest University College of Agronomy and BiotechnologySouthwest UniversityBeibeiChongqing 400715China Engineering Research center of South Upland Agriculture Ministry of EducationChongqing 400715China Academy of Agricultural Sciences Southwest UniversityBeibeiChongqing 400715China China Golden Marker(Beijing)biotech Co. Ltd.Beijing 102206China

Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding ***,the complexity of its polyploid genome increases sequencing costs and reduces sequencing *** capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of *** this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named *** platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic *** capture quality of BnaPan50T was demonstrated well in 12 typical *** with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide *** capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the ***50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification *** study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.

关键词： Rapeseed Target capture SNP genotyping platform Genomic breeding

Three-dimensional solution structure of ω-conotoxin SO_(3) determined by ^(1)H NMR

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Chinese Science Bulletin 2003年第11期48卷 1097-1102页

作者： YAN Yongbin TU Guangzhong LUO Xuechun DAI Qiuyun HUANG Peitang ZHANG Riqing NMR Laboratory Department of Biological Sciences and Biotech-nologyTsinghua UniversityBeijing 100084China Analytical center Beijing Institute of MicrochemistryBeijing 100091China Institute of biotechnology Beijing 100071China State Key Laboratory of Biomembrane and Membrane biotechnology Tsinghua UniversityBeijing 100084China

Cone snails (Conus) elaborate a series of conotoxin (CTX) peptides in their venoms to paralyze their prey. Among these toxins, w-CTX抯 specifically target to presynaptic voltage-gated calcium channel subsets, causing inhibition of neurotransmitter release. w-CTX SO3 was isolated from the venom of Conus striatus, which is the only available fish-hunting snail near the coast of the South China Sea. The three-dimensional solution structure of w-CTX SO3, a peptide which is the only w-conotoxin reported to show high homology with another w-CTX (MVIIA from C. Magus), has been determined by 1H NMR techniques. The molecular structure of w-CTX SO3 is stabilized by three disulfide bridges and a short triple-stranded antiparallel b-sheet with four turns. A comprehensive comparison suggested that the backbone conformation of w-CTXs was quite conserved, while the length of b-sheet and the type of some turns might have minor differences.

关键词：核磁共振毒液神经传递素 ω-CTXSO3 神经毒素蜗牛

Duplication and expression analysis of multicopy miRNA gene family members in Arabidopsis and rice

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Cell Research 2006年第5期16卷 507-518页

作者： Danhua Jiang~(1,2) Changsong Yin~1 Aiping Yu~(1,3) Xiaofan Zhou~1 Wanqi Liang~(1,4) Zheng Yuan~1 Yun Xu~1 Qingbo Yu~1 Tieqiao Wen~2 Dabing Zhang~(1,4) ~1SJTU-SIBS-PSU Joint center for Life Sciences,School of Life Science and biotechnology,Shanghai Jiao Tong University 800 Dongchuan Road,Shanghai 200240, China ~2School of Life Science,Shanghai University,99 Shangda Road,Shanghai 200444,China ~3College of Life and Environment Sciences,Shanghai Normal University,100 Guilin Road, Shanghai 200234,China ~4Key Laboratory of Agricultural Genetics and Breeding,Agro-biotech Research center,Shanghai Academy of Agricultural Sciences,2901 Beidi Road,Shanghai 201106,China SJTU-SIBS-PSU Joint center for Life Sciences School of Life Science and Biotechnology Shanghai Jiao Tong University Shanghai China School of Life Science Shanghai University Shanghai China College of Life and Environment Sciences Shanghai Normal University Shanghai China Key Laboratory of Agricultural Genetics and Breeding Agro-biotech Research Center Shanghai Academy of Agricultural Sciences Shanghai China

To understand the expansion of multicopy microRNA (miRNA) families in plants,we localized the reported miRNAgenes from Arabidopsis and rice to their chromosomes,respectively,and observed that 37% of 117 miRNA genes fromArabidopsis and 35% of 173 miRNA genes from rice were segmental duplications in the *** order to characterizewhether the expression diversification has occurred among plant multicopy miRNA family members,we designed PCRprimers targeting 48 predicted miRNA precursors from 10 families in Arabidopsis and *** from RT-PCR datasuggest that the transcribed precursors of members within the same miRNA family were present at different *** addition,although miR160 and miR162 sequences were conserved in Arabidopsis and rice,we found that theexpression patterns of these genes differed between the two *** data suggested that expression diversifica-tion has occurred in multicopy miRNA families,increasing our understanding of the expression regulation of miRNAsin plants.

关键词： gene duplication microRNA multicopy