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TRIM25 inhibits HBV replication by promoting HBx degradation and the RIG-I-mediated pgRNA recognition

Chinese Medical Journal 2023年第7期136卷 799-806页

作者： Hongxiao Song Qingfei Xiao Fengchao Xu Qi Wei Fei Wang Guangyun Tan Department of Hepatology Center for Pathogen Biology and Infectious DiseasesInstitute of Translational MedicineThe First Hospital of Jilin UniversityChangchunJilin 130000China Department of Nephrology The First Hospital of Jilin UniversityChangchunJilin 130000China Department of Anesthesiology The First Hospital of Jilin UniversityChangchunJilin 130000China

Background:The hepatitis B virus(HBV)vaccine has been efficiently used for ***,hepatocellular carcinoma caused by HBV is still prevalent *** previously reported that interferon(IFN)-induced tripartite motif-containing 25(TRIM25)inhibited HBV replication by increasing the IFN expression,and this study aimed to further clarify the anti-HBV mechanism of ***:The TRIM25-mediated degradation of hepatitis B virus X(HBx)protein was determined by detecting the expression of HBx in TRIM25-overexpressed or knocked-out HepG2 or HepG2-NTCP cells via Western ***-immunoprecipitation was performed to confirm the interaction between TRIM25 and HBx,and colocalization of TRIM25 and HBx was identified via immunofluorescence;HBV e-antigen and HBV surface antigen were qualified by using an enzyme-linked immunosorbent assay(ELISA)kit from Kehua ***25 mRNA,pregenomic RNA(pgRNA),and HBV DNA were detected by quantitative real-time polymerase chain *** retinoic acid-inducible gene I(RIG-I)and pgRNA interaction was verified by RNA-binding protein immunoprecipitation ***:We found that TRIM25 promoted HBx degradation,and confirmed that TRIM25 could enhance the K90-site ubiquitination of HBx as well as promote HBx degradation by the proteasome ***,apart from the Really Interesting New Gene(RING)domain,the SPRY domain of TRIM25 was also indispensable for HBx *** addition,we found that the expression of TRIM25 increased the recognition of HBV pgRNA by interacting with RIG-I,which further increased the IFN production,and SPRY,but not the RING domain is critical in this ***:The study found that TRIM25 interacted with HBx and promoted HBx-K90-site ubiquitination,which led to HBx *** the other hand,TRIM25 may function as an adaptor,which enhanced the recognition of pgRNA by RIG-I,thereby further promoting IFN *** study can contribute to a better understanding of host-vir

关键词： HBx Hepatitis B virus pgRNA RIG-I TRIM25

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Safety and efficacy of tenecteplase versus alteplase in patients with acute ischaemic stroke (TRACE): a multicentre, randomised, open label, blinded-endpoint (PROBE) controlled phase II study

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Stroke & Vascular Neurology 2022年第1期7卷 47-53页

作者： Shuya Li Yuesong Pan Ziran Wang Zhigang Liang Huisheng Chen Dong Wang Yi Sui Xingquan Zhao Yilong Wang WanLiang Du Huaguang Zheng Yongjun Wang China National Clinical Research center for Neurological Diseases Beijing Tiantan HospitalBeijingChina Department of Neurology Beijing Tiantan HospitalBeijingChina Department of Neurology Linyi People's HospitalLinyiChina Department of Neurology Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical CollegeYanTaiChina Department of Neurology General Hospital of Northern Theatre CommandShenyangLiaoningChina Department of Neurology Baogang Hospital of Inner MongliaInner MongliaChina Department of Neurology The First People’s Hospital of ShenyangShenyangChina Neurology Beijing Tiantan HospitalBeijingChina

Background Tenecteplase(TNK)possesses several pharmacological characteristics superior to conventional alteplase(rt-PA),with well-established safety and efficacy profile in *** exists controversy over the optimal dose of intravenous rt-PA for East Asians with acute ischaemic stroke(AIS).Current study aimed to determine the safety dose range of recombinant human TNK tissue-type plasminogen activator(rhTNK-tPA)for patients with AIS in *** This multicentre,prospective,randomised,open-label,blinded end-point,phase II study compared three tiers of 0.1,0.25,0.32 mg/kg rhTNK-tPA(to a maximum of 40 mg)with standard 0.9 mg/kg rt-PA(to a maximum of 90 mg)in patients who were eligible for intravenous *** safety outcome were symptomatic intracranial haemorrhage(sICH)within 36 *** Between May 2018 and February 2020,240 patients were randomly assigned to four group,4 of whom did not receive study *** intention-to-treat analysis included 236 *** was no difference in the improvement on National Institutes of Health Stroke Scale at day 14 in the 3 tiers and control group(63.3%,77.2%,66.7%vs 62.7%).The number of sICH was 3 of 60(5.0%)in the 0.1 mg/kg group,none in the 0.25 mg/kg group,2 of 60(3.3%)in the 0.32 mg/kg group and 1(1.7%)of 59 in the rt-PA *** were no significant between-group differences in severe adverse *** Similar to the Caucasians,rhTNK-tPA was well tolerated in Chinese patients with AIS at all doses administered within 3 hours of symptom *** dose-efficacy profile of rhTNK-tPA needs to be established with future investigations.

关键词： patients doses centre

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Notes for genera:Ascomycota

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Fungal Diversity 2017年第5期86卷 1-594页

作者： Nalin N.Wijayawardene Kevin D.Hyde Kunhiraman C.Rajeshkumar David L.Hawksworth Hugo Madrid Paul M.Kirk Uwe Braun Rajshree V.Singh Pedro W.Crous Martin Kukwa Robert Lücking Cletus P.Kurtzman Andrey Yurkov Danny Haelewaters Andre´Aptroot H.Thorsten Lumbsch Einar Timdal Damien Ertz Javier Etayo Alan J.L.Phillips Johannes Z.Groenewald Moslem Papizadeh Laura Selbmann Monika C.Dayarathne Gothamie Weerakoon E.B.Gareth Jones Satinee Suetrong Qing Tian Rafael F.Castanda-Ruiz Ali H.Bahkali Ka-Lai Pang Kazuaki Tanaka Dong Qin Dai Jariya Sakayaroj Martina Hujslová Lorenzo Lombard Belle D.Shenoy Ave Suija Sajeewa S.N.Maharachchikumbura Kasun M.Thambugala Dhanushka N.Wanasinghe Bharati O.Sharma Subhash Gaikwad Gargee Pandit Laura Zucconi Silvano Onofri Eleonora Egidi Huzefa A.Raja Rampai Kodsueb Marcela E.S.Caceres Sergio Perez-Ortega Patrícia O.Fiuza Josiane Santana Monteiro Larissa N.Vasilyeva Roger G.Shivas Maria Prieto Mats Wedin Ibai Olariaga Adebola Azeez Lateef Yamini Agrawal Seyed Abolhassan Shahzadeh Fazeli Mohammad Ali Amoozegar Guo Zhu Zhao Walter P.Pfliegler Gunjan Sharma Magdalena Oset Mohamed A.Abdel-Wahab Susumu Takamatsu Konstanze Bensch Nimali Indeewari de Silva AndréDe Kesel Anuruddha Karunarathna Saranyaphat Boonmee Donald H.Pfister Yong-Zhong Lu Zong-Long Luo Nattawut Boonyuen Dinushani A.Daranagama Indunil C.Senanayake Subashini C.Jayasiri Milan C.Samarakoon Xiang-Yu Zeng Mingkwan Doilom Luis Quijada Sillma Rampadarath Gabriela Heredia Asha J.Dissanayake Ruvishika S.Jayawardana Rekhani H.Perera Li Zhou Tang Chayanard Phukhamsakda Margarita Hernańdez-Restrepo Xiaoya Ma Saowaluck Tibpromma Luis F.P.Gusmao Darshani Weerahewa Samantha C.Karunarathna Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand National Fungal Culture Collection of India(NFCCI) Biodiversity and Palaeobiology(Fungi)GroupAgharkar Research InstitutePuneMaharashtra 411004India Royal Botanic Gardens KewRichmondSurrey TW93DSUK Department of Life Sciences The Natural History MuseumCromwell RoadLondon SW75BDUK Jilin Agricultural University Changchun 130118JilinPeople’s Republic of China Centro de Geno´mica y Bioinforma´tica Facultad de CienciasUniversidad Mayor de ChileCamino La Pira´mide 5750HuechurabaSantiagoChile Martin-Luther-Universita¨t Institut fu¨r BiologieBereich Geobotanik und Botanischer GartenHerbariumNeuwerk 2106099 Halle(Saale)Germany Westerdijk Fungal Biodiversity Institute Uppsalalaan 83584 CT UtrechtThe Netherlands Department of Plant Taxonomy and Nature Conservation Faculty of BiologyUniversity of Gdan´skWita Stwosza 5980-308 Gdan´skPoland Botanic Garden and Botanical Museum Königin-Luise-Str.6-814195 BerlinGermany Mycotoxin Prevention and Applied Microbiology Research Unit National Center for Agricultural Utilization ResearchAgricultural Research ServiceU.S.Department of AgriculturePeoriaIL 61604USA Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Inhoffenstraße 7B38124 BrunswickGermany Farlow Reference Library and Herbarium of Cryptogamic Botany Harvard University22 Divinity AvenueCambridgeMA 02138USA Department of Organismic and Evolutionary Biology Harvard University22 Divinity AvenueCambridgeMA 02138USA ABL Herbarium G.v.d.Veenstraat 1073762 XK SoestThe Netherlands Science&Education The Field Museum1400

Knowledge of the relationships and thus the classification of fungi,has developed rapidly with increasingly widespread use of molecular techniques,over the past 10–15 years,and continues to *** genera have been found to be polyphyletic,and their generic concepts have subsequently been *** names have thus been introduced for species which are phylogenetically distinct from the type species of particular *** ending of the separate naming of morphs of the same species in 2011,has also caused changes in fungal generic *** order to facilitate access to all important changes,it was desirable to compile these in a single *** present article provides a list of generic names of Ascomycota(approximately 6500 accepted names published to the end of 2016),including those which are *** and summaries of the changes since the last edition of‘Ainsworth&Bisby’s Dictionary of the Fungi’in 2008 are *** notes include the number of accepted species,classification,type species(with location of the type material),culture availability,life-styles,distribution,and selected publications that have appeared since *** work is intended to provide the foundation for updating the ascomycete component of the"Without prejudice list of generic names of Fungi"published in 2013,which will be developed into a list of protected generic *** will be subjected to the XIXth International Botanical Congress in Shenzhen in July 2017 agreeing to a modification in the rules relating to protected lists,and scrutiny by procedures determined by the Nomenclature Committee for Fungi(NCF).The previously invalidly published generic names Barriopsis,Collophora(as Collophorina),Cryomyces,Dematiopleospora,Heterospora(as Heterosporicola),Lithophila,Palmomyces(as Palmaria)and Saxomyces are validated,as are two previously invalid family names,Bartaliniaceae and *** species of Lalaria,which were invalidly published are trans

关键词： Article 59 Classification One name Pleomorphic fungi Taxonomy

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Patella Alta and Trochlea Dysplasia Is Associated with Abnormal Type Ⅱ Collagen and Matrix Accumulation in Chondrocytes

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Microscopy Research 2014年第2期2卷 19-29页

作者： Peter Storgaard Skagen Thomas Horn Aubrey Milunsky David Dejour Bente St█rgaard Hanne Aagaard Kruse Tom Nicolaisen Department of Sports Traumatology Frederikssund HospitalFrederikssundDenmark Department of Pathology Herlev University HospitalHerlevDenmark Boston University School of Medicine Center for Human GeneticsBostonUSA Department of Orthopaedic&Sportmedicine Surgery Lyon-Ortho-Clinic LyonFrance

The purpose of this study was to perform morphological and molecular analyses of articular cartilage from a 14-year-old boy with unusual cartilage lesions, patella alta and trochlea dysplasia in both knee joints and clinically examine two family members (sister, mother), also affected in their knee joints. Biopsies from the boy’s patella were used for: histological examination, Transmission Electron Microscopy (TEM) and DNA sequencing of the COL2A1 gene including Multiplex Ligation-dependent Probe Amplification (MLPA), for detection of DNA deletions and duplications. Clinical and radiological examination showed patella alta and trochlea dysplasia for the brother (type D), sister (type A) and mother (type A) with Insall-Salvati ratios of 1.50, 1.46 and 1.3. Light Microscopy (LM) of biopsies from the patient showed rhomboid chondrocytes in lacuna with deposition of protein aggregates in the ECM. TEM revealed abnormal type II collagen fibrils in aggregates and chondrocytes with abnormal matrix accumulation in rough Endoplasmic Reticulum (rER). Immunostaining showed that type II collagen was deposited intracellularly and in protein aggregates, together with type I collagen, indicating alterations in chondrocyte function and turnover of these molecules. DNA sequencing of 54 exons including extended DNA analysis with MLPA was non-conclusive. Conclusions: We suggest that patella alta and trochlea dysplasia for this patient is associated with collagen accumulation in chondrocytes, abnormal type II collagen heterofibrils in the ECM, cell death and cartilage with subnormal strength and increased risk of premature patellofemoral arthritis. A family with these disorders suggests that phenotype might be transmitted as an autosomal dominant trait.

关键词： Patella Alta Trochlea Dysplasia Matrix Accumulation Endoplasmic Reticulum Storage Disease(ERSD)

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Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification

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Synthetic and Systems biotechnology 2016年第3期1卷 150-157页

作者： Hui Tao Yuchen Zhang Xiaoying Cao Zixin Deng Tiangang Liu Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of EducationWuhan University School of Pharmaceutical SciencesWuhan 430071P.R.China Hubei Engineering Laboratory for Synthetic Microbiology Wuhan Institute of BiotechnologyWuhan 430075P.R.China State Key Laboratory of Microbial Metabolism School of Life Sciences and BiotechnologyShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240P.R.China Hubei Provincial Cooperative Innovation center of Industrial Fermentation Wuhan 430068P.R.China

With worldwide attention on renewable energy and climate change,metabolic engineering of the fatty acid biosynthetic pathway has become an active area of research,with a view to enhance production of ***,this pathway has already been extensively studied in Escherichia ***,little is known about the absolute abundance of the enzymes involved,information that may be valuable for engineering,such as the optimal molar ratios of different *** this study,we use protein standard absolute quantification(PSAQ)to measure the absolute abundance of proteins that catalyze fatty acid biosynthesis in *** addition,the changes of protein abundance were analyzed by comparing the differences between high-yield and the background *** work highlights opportunities to enhance fatty acid production by measuring protein molar ratios and identifying catalytic and regulatory *** importantly,our results provide evidence that PSAQ is a generally valuable tool to investigate metabolic pathways.

关键词： Fatty acid Absolute quantification Proteomics MRM PSAQ

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美国生物技术产业2002～2003概貌

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高技术通讯 2003年第10期13卷 107-110页

作者：郑永和吴越黎志良中国自然科学基金会 Burrill&Comang One Embarcadero center AFG Biophama P.O.Box7311Fremont CA94537USA

对 2 0 0 2年美国的生物技术产业现状进行了综述。分析了影响美国生物技术产业的因素 ,概述了美国生物技术产业特征及 2 0 0

关键词：美国生物技术产业股票市场生物制药风险投资

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Preserving cognitive function in patients with Alzheimer's disease:The Alzheimer's disease neuroprotection research initiative(ADNRI)

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Neuroprotection 2023年第2期1卷 84-98页

作者： Jie Liu Heleen van Beusekom Xian-Le Bu Gong Chen Paulo Henrique Rosado de Castro Xiaochun Chen Xiaowei Chen Andrew N.Clarkson Tracy D.Farr Yuhong Fu Jianping Jia Jukka Jolkkonen Woojin Scott Kim Paula Korhonen Shen Li Yajie Liang Guang-Hui Liu Guiyou Liu Yu-Hui Liu Tarja Malm Xiaobo Mao Joaquim Miguel Oliveira Mike M.Modo Pedro Ramos-Cabrer Karsten Ruscher Weihong Song Jun Wang Xuanyue Wang Yun Wang Haitao Wu Lize Xiong Yi Yang Keqiang Ye Jin-Tai Yu Xin-Fu Zhou Marietta Zille Colin L.Masters Piotr Walczak Boltze Johannes Xunming Ji Yan-Jiang Wang Department of Neurology Daping HospitalThird Military Medical UniversityChongqingChina Chongqing Key Laboratory of Ageing and Brain Diseases ChongqingChina Division of Experimental Cardiology Department of CardiologyErasmus MCUniversity Medical CenterRotterdamThe Netherlands Institute of Brain and Intelligence Third Military Medical UniversityChongqingChina Guangdong-HongKong-Macao Institute of CNS Regeneration(GHMICR) Jinan UniversityGuangzhouGuangdongChina Institute of Biomedical Sciences Federal University of Rio de JaneiroRio de JaneiroRio de JaneiroBrazil Fujian Key Laboratory of Molecular Neurology Department of Neurology and GeriatricsFujian Institute of GeriatricsFujian Medical University Union HospitalInstitute of NeuroscienceFujian Medical UniversityFuzhouFujianChina Guangyang Bay Laboratory Chongqing Institute for Brain and IntelligenceChongqingChina center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghaiChina Department of Anatomy Brain Health Research Centre and Brain Research New ZealandUniversity of OtagoDunedinNew Zealand School of Life Sciences University of NottinghamNottinghamUK Brain and Mind Centre&School of Medical Sciences The University of SydneySydneyNew South WalesAustralia Innovation center for Neurological Disorders and Department of Neurology Xuanwu HospitalNational Clinical Research Center for Geriatric DiseasesCapital Medical UniversityBeijingChina A.I.Virtanen Institute for Molecular Sciences University of Eastern FinlandKuopioFinland Department of Neurology and Psychiatry Beijing Shijitan HospitalCapital Medical UniversityBeijingChina Department of Diagnostic Radiology and Nuclear Medicine University of Maryland School of MedicineBaltimoreMarylandUSA University of Chinese Academy of Sciences BeijingChina State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Sci

The global trend toward aging populations has resulted in an increase in the occurrence of Alzheimer's disease(AD)and associated socioeconomic *** metabolism of amyloid-β(Aβ)has been proposed as a significant pathomechanism in AD,supported by results of recent clinical trials using anti-Aβ***,the cognitive benefits of the current treatments are *** etiology of AD is multifactorial,encompassing Aβand tau accumulation,neuroinflammation,demyelination,vascular dysfunction,and comorbidities,which collectively lead to widespread neurodegeneration in the brain and cognitive ***,solely removing Aβfrom the brain may be insufficient to combat neurodegeneration and preserve *** attain effective treatment for AD,it is necessary to(1)conduct extensive research on various mechanisms that cause neurodegeneration,including advances in neuroimaging techniques for earlier detection and a more precise characterization of molecular events at scales ranging from cellular to the full system level;(2)identify neuroprotective intervention targets against different neurodegeneration mechanisms;and(3)discover novel and optimal combinations of neuroprotective intervention strategies to maintain cognitive function in AD *** Alzheimer's Disease Neuroprotection Research Initiative's objective is to facilitate coordinated,multidisciplinary efforts to develop systemic neuroprotective strategies to combat *** aim is to achieve mitigation of the full spectrum of pathological processes underlying AD,with the goal of halting or even reversing cognitive decline.

关键词： Alzheimer's disease early intervention neural regeneration neuroprotection systematic perspective

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Inhibition of NF-kB and Wnt/β-catenin/GSK3p Signaling Pathways Ameliorates Cardiomyocyte Hypertrophy and Fibrosis in Streptozotocin (STZ)-induced Type 1 Diabetic Rats

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Current Medical Science 2020年第1期40卷 35-47页

作者： Jing-jing LIU Lu-mei SHENTU Ning MA Li-ying WANG Gui-min ZHANG Ying SUN Yan WANG Jun LI Yan-ling MU School of Medicine and Life Sciences University of Jinan-Shandong Academy of Medical SciencesJinan 250200China Institute of Materia Medica Shandong Academy of Medical SciencesJinan 250062China Key Laboratory for biotech-Drugs Ministry of Health Jinan 250062China Key Laboratory for Rare&Uncommon Diseases of Shandong Province Jinan 250062China Shandong Electric Power Central Hospital Jinan 250001China Laboratory Generic Manufacture Technology of Chinese Traditional MedicineJinan 250001China center for New Drug Pharmacological Research of Liman Pharmaceutical Group Jinan 250001China

Type 1 diabetes mellitus(T1DM)is associated with an increased risk of diabetic cardiomyopathy(DCM).Nuclear factor kappa B(NF-kB)and Wnt/β-catenin/GSK3p have been demonstrated to play pathogenic roles in *** this study,we evaluated the roles of these two pathways in T1 DM-induced cardiomyopathy in ***(STZ)-induced type 1 diabetic rats were treated with pyrrolidine dithiocarbamate(PDTC)or meisoindigo(Me)to inhibit NF-kB and Wnt/β-catenin/GSK3P respectively for 4 or 8 *** compared with untreated diabetic rats,treatment with either PDTC or Me partly attenuated the myocardial hypertrophy and interstitial fibrosis,improved cardiac function,and exhibited reduction in inflammatory *** addition,we found that inhibiting NF-κB and Wnt/β-catenin/GSK3β pathways could regulate glucose and lipid *** effects were associated with the decrease of NF-κB activity and the downregulation of some proinflammatory cytokines,including tumor necrosis factor-alpha(TNF-α)and interleukin(IL)-*** data suggested that the activities of NF-κB and Wnt/β-catenin/GSK3β pathways were both increased and inhibiting NF-κB and Wnt/β-catenin/GSK3β signaling pathways might improve myocardial injury in T1DM rats.

关键词： type 1 diabetes mellitus diabetic cardiomyopathy NF-κB Wnt/β-catenin/GSK3β

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Synergistic Antitumoral Effect of Paclitaxel（TAXOL）+AS101 In A Murine Model of B16 Melanoma:Association with Ras-dependent Signal Transduction Pathways

Synergistic Antitumoral Effect of Paclitaxel（TAXOL）+AS101 ...

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Asian-Pacific Conference of Tumor Biology 2001

作者： Y.Kalechman D.L.Longo R.Catane A.Shani M.Albeck B.Sredni C.A.I.R.Institute Fatulty of Life Sciences Bar llan UniversityRamat Gan52900 Israel Gerontology Research center NIANIHBaltimoreMD 21224-6825USA Department of Oncology Hadassah Medical CenterJerusalemIsrael Department of Oncology Kaplan HospitalRehovotIsrael C.A.I.R.Institute Fatulty of Life Sciences Bar llan UniversityRamat Gan52900 Israel C.A.I.R.Institute Fatulty of Life Sciences Bar llan UniversityRamat Gan52900 Israel

＜正＞Optimal doses of Taxol combined with the immunomodulator AS101,previously shown to have antitu-moral effects,administered to B16 melanoma-bearing mice,decreased tumor volume and resulted inover 60% ***+AS101 directly inhibited clonogenicity of B16 melanoma cells in a synergistic dose-dependent *** suggest that this results from both the reduced Taxol-induced BM toxicity andthe induction of differential signal transduction pathways which lead to apoptosis of tumor cells. Taxol+AS101 synergistically activated c-raf-1 and the MAPK ERK1 and *** activation was essential for

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Comparative transcriptome analysis during early fruit development between three seedy citrus genotypes and their seedless mutants

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Horticulture Research 2017年第1期4卷 142-153页

作者： Shujian Zhang Qingchun Shi Ute Albrecht Robert G Shatters Jr Ric Stange Greg McCollum Shuo Zhang Chengming Fan Ed Stover U.S.horticultural research laboratory usda-arsft.pierce34945flusa Institute of genetics and developmental biology chinese academy of sciencesBeijing100101china Horticultural sciences department southwest florida research and education centeruniversity of FloridaimmokaleeFL 34142USA

Identification of genes with differential transcript abundance(GDTA)in seedless mutants may enhance understanding of seedless citrus *** analysis was conducted at three time points during early fruit development(Phase 1)of three seedy citrus genotypes:Fallglo(Bower citrus hybrid(Citrus reticulata×***×***)×Temple(***×***)),grapefruit(***),Pineapple sweet orange(***),and their seedless *** abortion in seedless mutants was observed at 26 days post anthesis(Time point 2).Affymetrix transcriptomic analysis revealed 359 to 1077 probe sets with differential transcript abundance in the comparison of seedless versus seedy fruits for each citrus genotypes and time *** GDTA identified by 18 microarray probe sets were validated by *** clustering analysis revealed a range of GDTA associated with development,hormone and protein metabolism,all of which may reflect genes associated with seedless fruit *** were 14,9 and 12 genes found exhibiting similar abundance ratios in all three seedless versus seedy genotype comparisons at time point 1,2 and 3,*** those genes were genes coding for an aspartic protease and a cysteine protease,which may play important roles in seedless fruit *** insights into seedless citrus fruit development may contribute to biotech approaches to create seedless cultivars.

关键词： sinensis citrus cultivar

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