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Soybean Seed Protein, Oil, Fatty Acids, and Isoflavones Altered by Potassium Fertilizer Rates in the Midsouth

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American Journal of Plant Sciences 2013年第5期4卷 976-988页

作者： Nacer Bellaloui Xinhua Yin Alemu Mengistu Angela M. McClure Don D. Tyler Krishna N. Reddy Crop Genetics Research Unit USDA-ARS Jackson USA Crop Genetics Research Unit USDA-ARS Stoneville USA Crop Production Systems Research Unit USDA-ARS Stoneville USA. Department of biosystems engineering and Soil Science The University of Tennessee Jackson USA Department of Plant Sciences The University of Tennessee Jackson USA Department of Plant Sciences University of Tennessee Jackson USA

Previous research has shown that the effect of potassium fertilizer on soybean ([Glycine max (L.) Merr.] seed composition (protein, oil, fatty acids, and isoflavones) is still largely unknown. Therefore, the objective of this research was to investigate the effects of potassium application on seed protein, oil, fatty acids, and isoflavones under Midsouth environmental conditions. A three-year experiment was conducted in two locations (Milan, TN and Jackson, TN). Potassium (K) rates were applied in the form of K2O at a rate of 0 (Control, C), 45 (T1), 90 (T2), 134 (T3), and 179 (T4) kg·ha-1 in a randomized complete block design. The results showed that increasing the K application rate did not result in consistent effects on yield. However, increasing K application rate did increase protein, oleic acid, and individual and total isoflavone concentrations at both locations in 2008 and 2009. In Jackson in 2010, the increase of K rate did not change oleic acid, but resulted in an increase in glycitein and genistein isoflavone concentrations. In 2010, increasing K application rate increased protein concentrations, decreased individual and total isoflavones, and did not change oleic acid concentration at Milan. At the highest rate of K, 179 kg·ha-1, yield and some seed composition constituents were negatively impacted. Generally, K concentration in leaves at V5, R1, R3, and seed at harvest maturity stage (R8) increased with the increase of K rate applications. The research demonstrated that K application can alter seed composition, but this alteration depended on location, environmental stress factors, mainly heat and drought, K level in soil, and K application rate. Higher rates of K application may negatively impact seed composition constituents.

关键词： Fatty Acids Isoflavones Oil Oleic Acid Potassium Fertilizer Protein Seed Composition

Intestinal microbiota and oral administration of Enterococus faecium associated with the growth performance of newborn piglets

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Intestinal microbiota and oral administration of Enterococus...

中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会

作者： Y.B.Wang W.Du A.K.Fu X.P.Zhang Y.Huang K.H.Lee K.Yu W.F.Li Y.L.Li College of Animal Science Zhejiang University National engineering Laboratory of Bio Feed Safety and pollution prevention Key Laboratory of Molecular Animal NutritionMinistry of EducationKey Laboratory of Animal Nutrition and Feed Science in East ChinaMOA College of biosystems engineering and Food Science Zhejiang University College of Animal Science and Technology Guangxi University Faculty of Medicine Dentistry and Health ScienceThe University of Western Australia

The oral administration of Enterococus faecium(EFl) to newborn suckling and weaned piglets along with their growth performances and intestinal microbiota was investigate in this study..Twenty-four newborn piglets were initially divided into 2 *** treatment or probiotics group receiving 2 ml of 10%sterilized skim milk by oral gavage was supplemented with viable Enterococcus faecium EF1(6×10 CFU/mL) at the first,the third and the fifth day after birth,while the control group was made of 2 ml of 10%sterilized skim milk without probiotic at the same *** showed that oral administration of Enterococcus faecium EF1 was associated with a remarkable increaseon the body weight of piglets for both suckling and weaning periods,by 30.73%(P<0.01) and 320.84%(P<0.01),and also decreased the diarrhea rate,by 43.21%(P<0.05) and 71.42%(P<0.05),*** addition,454-pyrosequencing analysis revealed that there was no significant difference in the intestinal microbial diversity of the suckling piglets between the two groups,nevertheless,when compared to the control group,the relative abundance of Firmicutes in the probiotics group substantially augmentedy,while the relative abundance of Proteobacteria,Bacteroidetes and Fusobacteria ***,result indicated that oral administration of Enterococus faecium EF1 did not have any influence on the relative abundance of Firmicutes in weaned piglets rather than increasing the relative abundance of Bacteroidetes and decreasing the relative abundance of ***,at level of Firmicutes phylum,the relative abundance of Lactobacillales in theprobiotic group increased *** findings suggest that oral administration of *** EF1 to newborn piglets could improve the growth performance and intestinal microbiota of piglets for both suckling and weaning periods.

关键词： Enterococus faecium EF1 piglets growth performance microbiota

食物-能源-水钮带关系研究能否与农业创新保持同步?

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engineering 2023年第4期23卷 24-28页

作者：庄杰, Tom Gill Frank E.Löffler 金明洲 Gary S.Sayler Department of biosystems engineering and Soil Science The University of TennesseeKnoxvilleTN 37996USA Center for Environmental Biotechnology The University of TennesseeKnoxvilleTN 37996USA Institute for a Secure and Sustainable Environment The University of TennesseeKnoxvilleTN 37996USA Department of Agricultural Leadership Education and CommunicationsThe University of TennesseeKnoxvilleTN 37996USA Smith Center for International Agriculture The University of TennesseeKnoxvilleTN 37996USA Department of Microbiology The University of TennesseeKnoxvilleTN 37996USA Department of Civil and Environmental engineering The University of TennesseeKnoxvilleTN 37996USA Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA Department of Industrial and Systems engineering The University of TennesseeKnoxvilleTN 37996USA

***,The interconnection among food-energy-water(FEW)systems in meeting societal demands is broadly acknowledged[1].Similarly,competitive or synergistic allocations of water and energy resources for agricultural production,manufacturing,and human consumption are understood,and their economic impacts can be predicted[2].Far less appreciated and understood are the outcomes of the FEW nexus in response to operation changes in agricultural practices and the associated technological innovations for future generations[3,4].Also,the inter-scale and feedback effects of emerging technology-driven resource reallocation and decision-making on FEW systems are largely *** example,how do the agroeconomic feedbacks of intelligent technologies influence the FEW nexus of agricultural production under environmental and demographic changes?How does the necessary water allocation for powering non-powered dams and pumped-storage hydropower generation influence agricultural production and municipal water supply maintenance?How do solar and wind energy farms influence land use for agriculture and the rural economy?

关键词： agricultural understood allocation

Modeling of Soil Movement by Tillage

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Modeling of Soil Movement by Tillage

国际农业工程大会

作者： Jude Liu David A. Lobb Ph.D Department of biosystems engineering University of Manitoba Winnipeg Manitoba Canada R3T 2N2 Ph.D. Associate Professor Department of Soil Science University of Manitoba Winnipeg Manitoba Canada R3T 2N2.

＜正＞ The process of tillage tool-soil-crop residue interaction was described based on the video recorded in a soil bin and a field experiment using a 325-mm-wide sweep. Then, a three-dimensional model was developed to predict the soil displacement by the sweep. Soil displacement by tillage tool was simplified into three components: forced, projectile, and rolling displacement. The model was verified

关键词： Tillage Soil Movement Model

Selective binding of an organoruthenium complex to G-rich human telomeric sequence by tandem mass spectrometry

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Selective binding of an organoruthenium complex to G-rich hu...

中国化学会第十四届全国生物无机化学学术会议

作者： Yiyu Cheng Wenjuan Zeng Yang Cheng Jishuai Zhang Tao Zou Kui Wu Fuyi Wang School of Chemistry and Chemical engineering Wuhan University of Science and Technology Beijing National Laboratory for Molecular Sciences National Centre for Mass Spectrometry in Beijing CAS Key Laboratory of Analytical Chemistry for Living Biosystems CAS Research/Education Centre for Excellence in Molecular Sciences Institute of Chemistry Chinese Academy of Sciences University of Chinese Academy Sciences

RATIONALE: Human telomeric DNA is reported to be a potential target for anticancer organometallic ruthenium（Ⅱ） complexes, however, the interaction sites were not clearly discriminated and identified. METHODS: In current paper, tandem mass spectrometry by collisional induced dissociation（CID） was firstly introduced to identify the interaction sites of an organometallic ruthenium（II） complex [（η6-biphenyl）Ru（en）Cl][PF6]（1;en = ethylenediamine） with 5′-T1T2A3G4G5G6-3′（I）, the repeating unit of human telomeric DNA, under both positive-and negative-ion mode at a low reaction molar ratio（1/I = ethylenediamine） with 5′-T1T2A3G4G5G6-3′（I）, the repeating unit of human telomeric DNA, under both positiveand negative-ion mode at a low reaction molar ratio（1/I = 0.2） which was applied to preserve the site selectivity. RESULTS: Mass spectrometric results showed that mono-ruthenated I was the main product under conditions. Under positive-ion mode, MS/MS results indicated that ruthenium complex 1 binds to T2 or G6 in strand I. However under negative-ion mode, no efficient information was obtained for exact identification of ruthenation sites which may be attributed to losses of fragment ions due to charge neutralization by the coordination of positive-charged ruthenium complex to the short MS/MS fragments. CONCLUSION: This is the first report by using top-down MS to characterize the interactions of organometallic ruthenium（II） complexes and human telomeric DNA. Thymine can be thermodynamically-competitive with guanine for binding to ruthenium complexes even at low reaction molar ratio, which inspires us to deeply explore the significance of thymine binding.

关键词： organometallic ruthenium complex human telomeric DNA tandem mass spectrometry CID guanine/thymine

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GC-O技术在气味检测领域中的应用

GC-O技术在气味检测领域中的应用

恶臭污染控制与技术标准——第三届全国恶臭污染测试与控制技术暨恶臭污染物排放标准修订研讨会

作者：刘静张士成陈建民 Jacek Koziel 复旦大学环境科学与工程系 Department of Agricultural and biosystems engineering Iowa State UniversityAmesIA 50011USA

本文简要介绍了气相色谱-嗅觉计(gas chromatography-olfactometry,GC-O)的基本原理及检测方法,重点讨论了GC-O技术在食品、饮料等香味气体,以及工业污水处理厂、动物集中养殖场所等恶臭气体检测方面的新进展,并指出了GC-O的优缺点及未... 详细信息

本文简要介绍了气相色谱-嗅觉计(gas chromatography-olfactometry,GC-O)的基本原理及检测方法,重点讨论了GC-O技术在食品、饮料等香味气体,以及工业污水处理厂、动物集中养殖场所等恶臭气体检测方面的新进展,并指出了GC-O的优缺点及未来的发展方向。

关键词： GC-O 恶臭气体气味检测污水处理厂动物集中养殖

Liquid flow in scaffold derived from natural source:experimental observations and biological outcome

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Regenerative Biomaterials 2022年第1期9卷 480-489页

作者： Elisabetta Salerno Giulia Orlandi Claudio Ongaro Alessandro d’Adamo Andrea Ruffini Gianluca Carnevale Barbara Zardin Jessika Bertacchini Diego Angeli CNR-NANO S3 Research Center on Nanostructures and biosystems at Surfaces Modena I-41125Italy Department of Sciences and Methods for engineering University of Modena and Reggio EmiliaReggio Emilia 42122Italy Department of Surgery MedicineDentistry and Morphological Sciences with Interest in TransplantOncology and Regenerative MedicineUniversity of Modena and Reggio EmiliaModena 41125Italy DIEF-engineering Department“Enzo Ferrari” University of Modena and Reggio EmiliaModena 41125Italy Institute of Science and Technology for Ceramics(ISTEC) National Research Council(CNR)Faenza 48018Italy

This study investigates the biological effects on a 3D scaffold based on hydroxyapatite cultured with MC3T3 osteoblasts in response to flow-induced shear stress(FSS).The scaffold adopted here(B-HA)derives from the biomorphic transformation of natural wood and its peculiar channel geometry mimics the porous structure of the *** the point of view of fluid dynamics,B-HA can be considered a network of micro-channels,intrinsically offering the advantages of a microfluidic *** work,for the first time,offers a description of the fluid dynamic properties of the B-HA scaffold,which are strongly connected to its *** features are necessary to determine the FSS ranges to be applied during in vitro studies to get physiologically relevant *** selected ranges of FSS promoted the elongation of the attached cells along the flow direction and early osteogenic cell *** data confirmed the ability of B-HA to promote the differentiation process along osteogenic ***,such a bioactive and naturally derived scaffold can be considered as a promising tool for bone regeneration applications.

关键词： fluid shear stress flow resistance hydroxyapatite 3D scaffold

Direct electrification of silicon microfluidics for electric field applications

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微系统与纳米工程(英文) 2023年第3期9卷 145-157页

作者： Diego Monserrat Lopez Philipp Rottmann Gabriel Puebla-Hellmann Ute Drechsler Marcel Mayor Sven Panke Martin Fussenegger Emanuel Lörtscher IBM Research Europe-Zurich Säumerstrasse 4CH-8803 RüschlikonSwitzerland ETH Zürich Department of Biosystems Science and EngineeringMattenstrasse 264058 BaselSwitzerland University of Basel Department of ChemistrySt.Johanns-Ring 19CH-4056 BaselSwitzerland Institute for Nanotechnology(INT) Karlsruhe Institute of Technology(KIT)P.O.Box 364076021 KarlsruheGermany University of Basel Faculty of Life ScienceBaselSwitzerland

Microfluidic systems are widely used in fundamental research and industrial applications due to their unique behavior, enhanced control, and manipulation opportunities of liquids in constrained geometries. In micrometer-sized channels, electric fields are efficient mechanisms for manipulating liquids, leading to deflection, injection, poration or electrochemical modification of cells and droplets. While PDMS-based microfluidic devices are used due to their inexpensive fabrication, they are limited in terms of electrode integration. Using silicon as the channel material, microfabrication techniques can be used to create nearby electrodes. Despite the advantages that silicon provides, its opacity has prevented its usage in most important microfluidic applications that need optical access. To overcome this barrier, silicon-on-insulator technology in microfluidics is introduced to create optical viewports and channel-interfacing electrodes. More specifically, the microfluidic channel walls are directly electrified via selective, nanoscale etching to introduce insulation segments inside the silicon device layer, thereby achieving the most homogeneous electric field distributions and lowest operation voltages feasible across microfluidic channels. These ideal electrostatic conditions enable a drastic energy reduction, as effectively shown via picoinjection and fluorescence-activated droplet sorting applications at voltages below 6 and 15 V, respectively, facilitating low-voltage electric field applications in next-generation microfluidics.

关键词： injection walls overcome

Humidity-induced synaptic plasticity of ZnO artificial synapses using peptide insulator for neuromorphic computing

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Journal of Materials Science & Technology 2022年第24期119卷 150-155页

作者： Min-Kyu Song Hojung Lee Jeong Hyun Yoon Young-Woong Song Seok Daniel Namgung Taehoon Sung Yoon-Sik Lee Jong-Seok Lee Ki Tae Nam Jang-Yeon Kwon School of Integrated Technology Yonsei UniversityIncheon 21983South Korea Department of Materials Science and engineering Seoul National UniversitySeoul 08826South Korea Soft Foundry Seoul National UniversitySeoul 08826South Korea School of Chemical and Biological engineering Seoul National UniversitySeoul 08826South Korea

Neuromorphic devices inspired by the human brain have attracted significant attention because of their excellent ability for cognitive and parallel *** study presents ZnO-based artificial synapses with peptide insulators for the electrical emulation of biological *** demonstrated the dynamic responses of the device under various environmental *** proton-conducting property of the tyrosine-rich peptide enables time-dependent responses under ambient conditions such that various aspects of synaptic behaviors are emulated by the *** transition from short-term memory to longterm memory is achieved via electrochemical doping of ZnO by ***,we demonstrate an image classification simulation using a multi-layer perceptron model to evaluate the potential of the device for use in neuromorphic *** neural network based on our device achieved a recognition accuracy of 87.47% for the MNIST handwritten digit *** work proposes a novel device platform inspired by biosystems for brain-mimetic hardware systems.

关键词： Artificial synapse Neuromorphic computing Oxide semiconductor Proton conductor Artificial neural network