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An integrated microfluidic platform for nucleic acid testing

Microsystems & Nanoengineering 2024年第3期10卷 181-194页

作者： Antao Sun Petra Vopařilová Xiaocheng Liu Bingqian Kou TomášŘezníček TomášLednický Sheng Ni JiříKudr Ondřej Zítka Zdenka Fohlerová Petr Pajer Haoqing Zhang Pavel Neužil Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’anShaanxi 710072P.R.China Department of Chemistry and Biochemistry Mendel University in BrnoZemědělská161300 BrnoCzech Republic ITD Tech s.r.o Osvoboditelů100573581 BohumínCzech Republic Central European Institute of Technology Brno University of TechnologyPurkyňova 123Brno 61200Czech Republic Department of Electronic and Computer Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong Kong SARChina Department of Microelectronics Faculty of Electrical Engineering and CommunicationBrno University of TechnologyTechnická3058/10Brno 61600Czech Republic Military Health Institute U Vojenskénemocnice 120016200 Praha 6Czech Republic The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anShaanxi 710049P.R.China Bioinspired Engineering and biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049P.R.China

This study presents a rapid and versatile low-cost sample-to-answer system for SARS-CoV-2 *** system integrates the extraction and purification of nucleic acids,followed by amplification via either reverse transcriptionquantitative polymerase chain reaction(RT–qPCR)or reverse transcription loop-mediated isothermal amplification(RT–LAMP).By meeting diverse diagnostic and reagent needs,the platform yields testing results that closely align with those of commercial RT-LAMP and RT‒qPCR *** advantages of our system include its speed and *** assay is completed within 28 min,including sample loading(5 min),ribonucleic acid(RNA)extraction(3 min),and RT-LAMP(20 min).The cost of each assay is≈$9.5,and this pricing is competitive against that of Food and Drug Administration(FDA)-approved commercial *** some RNA loss during on-chip extraction is observed,the platform maintains a potential limit of detection lower than 297 *** makes the system particularly useful in environments where centralized laboratories are either unavailable or inconveniently *** key feature is the platform’s versatility,allowing users to choose between RT‒qPCR or RT‒LAMP tests based on specific requirements.

关键词： testing maintain versatile

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Self-Repairing Membranes for Inflatable Structures Inspired by a Rapid Wound Sealing Process of Climbing Plants

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Journal of Bionic Engineering 2011年第3期8卷 242-250页

作者： Markus Rampf Olga Speck Thomas Speck Rolf H. Luchsinger Empa Swiss Federal Laboratories.for Material Science and Technology Oberlandstrasse 129 8600 Diibendorf Switzerland Plant biomechanics Group Faculty of Biology Botanic Garden University of Freiburg Schainzlestrafle 179104 Freiburg Germany

A new self-repairing membrane for inflatable light weight structures such as rubber boats or Tensairity constructions is presented. Inspired by rapid self-sealing processes in plants, a thin soft cellular polyurethane foam coating is applied on the inside of a fabric substrate, which closes the fissure if the membrane is punctured with a spike. Experimental tests are carried out with a purpose built setup by measuring the air mass flow through a leak in a damaged membrane sample. It is shown that the weight per unit area of the self-repairing foam as well as the curing of the two component PU-foam under an overpressure influence the repair efficiency. Curing the foam under overpressure affects the relative density as well as the microstructure of the foam coatings. Maximal median repair efficiencies of 0.999 have been obtained with 0.16 *** 2 foam cured at 1 bar overpressure. These results suggest that the bio-inspired technique has the potential to extend the functional integrity of injured inflatable structures dramatically.

关键词： self-repair self-healing biomimetics inflatable structures puncture resistance PU foam coating

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动态系统理论的概念与技术在生物力学分析中的应用（英文）

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Journal of Sport and Health Science 2016年第1期5卷 3-13页

作者： Richard E.A.van Emmerik Scott W.Ducharme Avelino C.Amado Joseph Hamill Motor Control Laboratory Department of Kinesiology University of Massachusetts Amherst Amherst MA 01003 USA biomechanics laboratories Department of Kinesiology University of Massachusetts Amherst Amherst MA 01003 USA

Traditional biomechanical analyses of human movement are generally derived from linear *** these methods can be useful in many situations,they do not describe behaviors in human systems that are predominately *** this reason,nonlinear analysis methods based on a dynamical systems approach have become more prevalent in recent *** analysis techniques have provided new insights into how systems(1) maintain pattern stability,(2) transition into new states,and(3) are governed by short-and long-term(fractal) correlational processes at different spatio-temporal *** different aspects of system dynamics are typically investigated using concepts related to variability,stability,complexity,and *** purpose of this paper is to compare and contrast these different concepts and demonstrate that,although related,these terms represent fundamentally different aspects of system *** particular,we argue that variability should not uniformly be equated with stability or complexity of *** addition,current dynamic stability measures based on nonlinear analysis methods(such as the finite maximal Lyapunov exponent) can reveal local instabilities in movement dynamics,but the degree to which these local instabilities relate to global postural and gait stability and the ability to resist external perturbations remains to be ***,systematic studies are needed to relate observed reductions in complexity with aging and disease to the adaptive capabilities of the movement system and how complexity changes as a function of different task constraints.

关键词：系统概念技术非线性分析方法系统动力学不稳定性非线性行为自适应能力人体运动

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Mesenchymal stem cells and collagen patches for anterior cruciate ligament repair

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世界干细胞杂志：英文版（电子版） 2015年第2期7卷 521-534页

作者： Benjamin Gantenbein Neha Gadhari Samantha CW Chan Sandro Kohl Sufian S Ahmad Tissue and Organ Mechanobiology Institute for Surgical Technology and BiomechanicsUniversity of Bern EMPA Swiss Federal Laboratories for Materials Science and Technology Orthopedic Department Insel University Hospital

AIM: To investigate collagen patches seeded with mesenchymal stem cells(MSCs) and/or tenocytes(TCs) with regards to their suitability for anterior cruciate ligament(ACL) repair. METHODS: Dynamic intraligamentary stabilization utilizes a dynamic screw system to keep ACL remnants in place and promote biological healing, supplemented by collagen patches. How these scaffolds interact with cells and what type of benefit they provide has not yet been investigated in detail. Primary ACL-derived TCs and human bone marrow derived MSCs were seeded onto two different types of 3D collagen scaffolds, Chondro-Gide?(CG) and Novocart?(NC). Cells were seeded onto the scaffolds and cultured for 7 d either as a pure populations or as "premix" containing a 1:1 ratio of TCs to MSCs. Additionally, as controls, cells were seeded in monolayers and in co-cultures on both sides of porous high-density membrane inserts(0.4 μm). We analyzed the patches by real time polymerase chain reaction, glycosaminoglycan(GAG), DNA and hydroxyproline(HYP) content. To determine cell spreading and adherence in the scaffolds microscopic imaging techniques, i.e., confocal laser scanning microscopy(c LSM) and scanning electron microscopy(SEM), were ***: CLSM and SEM imaging analysis confirmed cell adherence onto scaffolds. The metabolic cell activity revealed that patches promote adherence and proliferation of cells. The most dramatic increase in absolute metabolic cell activity was measured for CG samples seeded with tenocytes or a 1:1 cell premix. Analysis of DNA content and c LSM imaging also indicated MSCs were not proliferating as nicely as tenocytes on CG. The HYP to GAG ratio significantly changed for the premix group, resulting from a slightly lower GAG content, demonstrating that the cells are modifying the underlying matrix. Real-time quantitativepolymerase chain reaction data indicated that MSCs showed a trend of differentiation towards a more tenogenic-like phenotype after 7 ***:

关键词： Anterior cruciate ligament rupture Anteriorcruciate ligament tenocyte Dynamic intraligamentarystabilization system Resazurin red assay Mesenchymalstem cells Real-time polymerase chain reaction Histology Scanning electron microscopy microscopy Scanning electron microscopy

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Tactile Imaging Markers to Characterize Female Pelvic Floor Conditions

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Open Journal of Obstetrics and Gynecology 2015年第9期5卷 505-515页

作者： Heather van Raalte Vladimir Egorov Princeton Urogynecology Princeton NJ USA Artann laboratories Trenton NJ USA

The Vaginal Tactile Imager (VTI) records pressure patterns from vaginal walls under an applied tissue deformation and during pelvic floor muscle contractions. The objective of this study is to validate tactile imaging and muscle contraction parameters (markers) sensitive to the female pelvic floor conditions. Twenty-two women with normal and prolapse conditions were examined by a vaginal tactile imaging probe. We identified 9 parameters which were sensitive to prolapse conditions (p < 0.05 for one-way ANOVA and/or p < 0.05 for t-test with correlation factor r from -0.73 to -0.56). The list of parameters includes pressure, pressure gradient and dynamic pressure response during muscle contraction at identified locations. These parameters may be used for biomechanical characterization of female pelvic floor conditions to support an effective management of pelvic floor prolapse.

关键词： biomechanics Soft Tissue Tactile Imaging Prolapse Tissue Elasticity Elastography

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Gait phase detection by using a portable system and artificial neural network

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Medicine in Novel Technology and Devices 2021年第4期12卷 41-49页

作者： Song-Hua Yan Yan-Cheng Liu Wei Li Kuan Zhang School of Biomedical Engineering Capital Medical UniversityBeijing100069China Beijing Key Laboratory of Fundamental Research on biomechanics in Clinical Application Capital Medical UniversityBeijing100069China Department of Bone and Soft Tissue Tumors Tianjin HospitalTianjin300211China Department of Rehabilitation Affiliated Hospital of Binzhou Medical CollegeShandong264003China

Gait phases are important to evaluate the walking function and to identify the characteristics of pathological ***,it is difficult to differentiate gait phases outside gait laboratories,thus,this study aimed to develop a method to detect 8 gait sub-phases using a wearable multiple sensor system and artificial neural network(ANN).Motion sensors were used to acquire the acceleration of lower limbs,and force sensitive resistors were used to detect contact state and force between the foot and the *** was recorded using a high-speed *** feed forward back-propagation(BP)neural networks were *** resilient BP algorithm was used to train ANN.A total of 66 volunteers participated in this *** the stance and swing phase detection,simulation of the training data showed an accuracy of 98.0%.The data from the test set showed a recognition accuracy of 97.75%.Because the ending point of the last phase‘Terminal Swing’is always 100%GC,we only listed seven *** prediction accuracy of seven phases were:35.9%,63.8%,93.6%,94.9%,94.8%,97.9%and 98%using the limb acceleration data *** average accuracy for seven phases were 68%,91.3%,97.8%,98.9%,98.8%,99.1%,and 99.5%using the limb acceleration and foot pressure data for fast,normal,and slow gait *** study provides a new method for eight gait sub-phases detection with high accuracy combining a wearable system and ANN,which may make gait phase analysis possible under free-living conditions.

关键词： Gait phases Wearable system Artificial neural network Acceleration Force sensitive resistor

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Viscoelasticity of amyloid plaques in transgenic mouse brain studied by Brillouin microspectroscopy and correlative Raman analysis

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Journal of Innovative Optical Health Sciences 2017年第6期10卷 3-16页

作者： Sara Mattana Silvia Caponi Francesco Tamagnini Daniele Fioretto Francesca Palombo Department of Physics and Geology University of Perugia Perugia I-06123Italy Istituto Officina dei Materiali del CNR(CNR-IOM)-Unita di Perugia Department of Physics and Geology University of Perugia Perugia I-06123Italy Hatherly laboratories Medical School University of ExeterExeter EX44PSUK School of Physics and Astronomy University of Exeter Exeter EX44QLUK

A myloidopathy is one of the most prominent hallmarks of Alkheimer's disease(AD),the leading cause of dementia worldwide,and is characterized by the accumulation of amyloid plaques in the brain *** plaques consist of abnornal deposits mainly composed of an aggregation-prone protein fragment,B-amyloid 140/1-42,into the extracellular *** micro-spectroscopy is an all-optical contactless technique that is based on the interaction between visible light and longitudinal acoustic waves or phonons,giving access to the viscoelasticity of a sample on a subcellular ***,we describe the first application of micromechanical mapping based on Brillouin scattering spectroscopy to probe the stifness of individual amyloid plaques in the hippocampal part of the brain of a B-amyloid overexpressi ng transgenic *** analysis based on Brillouin and Raman microspectroscopy showed that amyloid plaques have a complex structure with a rigid core of B-pleated shoet conformation(B-aryloid)protein sur-rounded by a softer ring-shaped region richer in lipids and other protein *** preliminary results give a new insight into the plaque biophysics and biomechanics,and a valuable contrast mechanism for the study and diagnosis of amnyloidopathy.

关键词： Alzheimer's imaging vibrational spectrosecopy protein misfolding light scattering

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