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Nano Research 2024年第1期18卷 586-596页

作者： Hanzhi Lu Mingrui Cui Yi Wang Xinran Du Xinyi Zhou Yuhan Fang Xiaoyan Gao Ying Peng Jianyong Zhu Guang Yang Fulun Li 1Department of Dermatology Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine Shanghai 200437 China 2Shanghai Engineering Research Center of Nano-biomaterials and Regenerative Medicine College of Biological Science and Medical Engineering Donghua University Shanghai 201620 China 3Clinical Laboratory Medicine Center Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine Shanghai 200437 China

Cutaneous nerve regeneration in diabetic wounds remains a challenge for current clinical treatment. Herein, a natural protein-based functional hydrogel is developed using keratin as the sole matrix, and protocatechuic aldehyde (PA) and zinc ions as the building blocks through dual-dynamic crosslinking reactions of thiol-aldehyde addition and catechol-zinc ions coordination. The unique structural design endows the hydrogel with excellent injectability, skin adhesion, self-healing and antibacterial ability, and biocompatibility. In addition, this hydrogel acts as a drug carrier for the loading and sustained release of phellopterin (PP), a natural compound extracted from traditional Chinese medicine Angelica dahurica. Our findings demonstrate that the PP-loaded hydrogel promotes cutaneous nerve regeneration, angiogenesis and tissue remodeling in the full-thickness wounds in both db/db and streptozotocin-induced C57BL/6 diabetic mice, notably by the synergistic effect of zinc ions and PP, showing a great potential in diabetic wound therapy.

关键词： functional hydrogel cutaneous nerve regeneration dynamic crosslinking diabetic wounds

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类器官芯片在生物医学中的研究进展

科学通报 2019年第9期64卷 902-910页

作者：吴谦潘宇祥万浩胡宁王平浙江大学生物医学工程与仪器科学学院生物传感器国家专业实验室生物医学工程教育部重点实验室杭州310027 中国科学院传感技术国家联合重点实验室上海200050 biomaterials Innovation Research Center Center for Biomedical Engineering Harvard Medical School

目前国际上药物筛选技术主要是依据药效学模型进行细胞和动物实验,测试给药前后的变化,但是这种初筛方法的效率并不够理想.近年来,随着生物技术和信息技术的迅速发展,使得类器官芯片成为生物医学中极具特色而富有活力的新兴领域.相比传... 详细信息

目前国际上药物筛选技术主要是依据药效学模型进行细胞和动物实验,测试给药前后的变化,但是这种初筛方法的效率并不够理想.近年来,随着生物技术和信息技术的迅速发展,使得类器官芯片成为生物医学中极具特色而富有活力的新兴领域.相比传统的平面细胞培养和动物模型,类器官具有更接近在体器官的结构和功能的优势,可以更好地反映人体生理学特性.结合类器官芯片与微流控技术构建的生理微系统,可以在体外模拟人体组织器官的主要结构和功能特征.本文综述了类器官的培养现状、器官芯片的制作和应用以及结合仿生传感技术的研究工作进展.这些技术研究在药物研发、疾病模型和个性化医疗方面具有广阔的应用前景.

关键词：类器官芯片仿生传感技术生物医学工程生物传感药物研发

A New and Enantioselective Chromatographic Method for Linagliptin on Amylose Coated Chiral Stationary Phase

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American Journal of Analytical Chemistry 2016年第7期7卷 556-567页

作者： Cholleti Vijay Kumar Pasula Aparna Pavan Kumar Vasa Y. Ravindra Kumar Nitin Haridas Dhekale Dr. Reddy’s laboratories Ltd. Active Pharmaceutical Ingredients IPDO Hyderabad India Department of Chemistry J. N. T. University Hyderabad India Department of Pediatrics SUNY Stony Brook Medical Center New York USA

A new and enantioselective liquid chromatographic method was developed for estimation of S-Linagliptin in Linagliptin (LINA) drug substances. The desired enantiomeric separation was achieved on Chiralpak AD-H (250*4.6 mm*5 μm) column with the mobile phase composition of ethanol, methanol and diethylamine in a ratio of 90:10:0.1 (v/v/v) with flow rate of 0.5 mL·min-1 and column oven temperature 30°C and the eluted compounds were monitored at 225 nm. In the proposed chiral method, USP resolutions between both the enantiomers were more than 5.0. Limit of detection and Limit of quantitation of S-LINA was found to be 0.03 μg·mL-1 and 0.10 μg·mL-1 respectively. Linearity study was conducted from LOQ to 150% and correlation coefficient found to be 0.9997. Accuracy was within the range of 98.6% to 101.5%. To prove selectivity power of the method specificity study was conducted by subjecting drug substance to acid, base, hydrolysis, oxidation and photolysis and ensured the peak purity of analyte in degraded samples. Moreover, the method has been fully validated as per ICH guidelines. The proposed method is precise, accurate, linear, rugged, robust and suitable for accurate quantification of S-LINA in LINA drug substance.

关键词： Enantiomers Amylose Based Stationary Phase Method Development Method Validation Specificity

Osteogenic differentiation of encapsulated cells in dexamethasone-loaded phospholipid-induced silk fibroin hydrogels

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biomaterials Translational 2022年第3期3卷 213-220页

作者： Chavee Laomeephol Helena Ferreira Sorada Kanokpanont Jittima Amie Luckanagul Nuno M Neves Siriporn Damrongsakkul Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand Biomaterial Engineering for Medical and Health Research Unit Chulalongkorn UniversityBangkokThailand B’s Research Group I3Bs-Research Institute on BiomaterialsBiodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineGuimarãesPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal Department of Chemical Engineering Faculty of EngineeringChulalongkorn UniversityBangkokThailand Biomedical Engineering Research Center Faculty of EngineeringChulalongkorn UniversityBangkokThailand Center of Excellence in Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand

The tissue engineering triad comprises the combination of cells,scaffolds and biological ***,we prepared cell-and drug-loaded hydrogels using in situ silk fibroin(SF)hydrogels induced by dimyristoyl glycerophosphoglycerol(DMPG).DMPG is reported to induce rapid hydrogel formation by SF,facilitating cell encapsulation in the hydrogel matrix while maintaining high cell viability and proliferative *** addition,DMPG can be used for liposome formulations in entrapping drug ***(Dex)was loaded into the DMPG-induced SF hydrogels together with human osteoblast-like SaOS-2 cells,then the osteogenic differentiation of the entrapped cells was evaluated in vitro and compared to cells cultured under standard *** production by cells cultured in DMPG/Dex-SF hydrogels with Dex-depleted osteogenic medium was equivalent to that of cells cultured in conventional osteogenic medium containing *** extended-release of the entrapped Dex by the hydrogels was able to provide a sufficient drug amount for osteogenic *** controlled release of Dex was also advantageous for cell viability even though its dose in the hydrogels was far higher than that in osteogenic *** results confirmed the possibility of using DMPG-induced SF hydrogels to enable dual cell and drug encapsulation to fulfil the practical applications of tissue-engineered constructs.

关键词： DMPG osteogenic differentiation silk fibroin three-dimensional cell culture tissue engineering

Prodrug design,synthesis and pharmacokinetic evaluation of(3'R,4'R)-3-hydroxymethyl-4-methyl-3',4'-di-O-(S)-camphanoyl-(t)-cis-khellactone

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Acta Pharmaceutica Sinica B 2012年第2期2卷 213-219页

作者： Huanfang Guo Xiaomei Zhuang Keduo Qian Lianqi Sun Xiaofeng Wang Hua Li Kuohsiung Lee Lan Xie Beijing Institute of Pharmacology and Toxicology Beijing 100850China Natural Products Research laboratories UNC Eshelman School of PharmacyUniversity of North CarolinaChapel HillNC 25799USA Chinese Medicine Research and Development Center China Medical University and HospitalTaichung 401

3-Hydroxymethyl-4-methyl-DCK(3,HMDCK)was discovered previously as a potent HIV non-nucleoside reverse transcriptase inhibitor(NNRTIs)(EC_(50):0.004 μM,TI:6225)with a novel mechanism of *** exerts anti-HIV activity by inhibiting the production of HIV-1 double-stranded viral DNA from a single-stranded DNA intermediate,rather than blocking the generation of single-stranded DNA from a RNA template,which is the mechanism of action of current HIV-1 RT ***,the insufficient metabolic stability of 3 limits its further clinical *** the current study,a series of ester prodrugs of 3 was designed and synthesized to explore the new drug candidates as *** L-alanine ester prodrug 10 exhibited desirable pharmacokinetic properties in vitro and in vivo and showed improved oral bioavailability of 26%in rat,and would be a potential clinical candidate as a new anti-AIDS drug.

关键词： 3-Hydroxymethyl-4-methyl-DCK Synthesis Pharmacokinetic Prodrug

Biomedical materials benefit health

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BMEMat(BioMedical Engineering Materials) 2023年第1期1卷 1-2页

作者： Hong Liu Haohai Yu Jianhua Li State Key Laboratory of Crystal Materials Shandong UniversityJinanChina Insttute for Advanced Interdisciplinary Research(LAIR) Untversity of JinanJinanChina Department of biomaterials School and Hospital of StomatologyCheeloo College of MedicineShandong Untversity&Shandong Key Laboratory of Oral Tissue Regeneration&Shandong Engineering aboratory for Dental Materials and Oral Tissue Regeneration&Shandong Provincial Clinical Research Center for Oral DiseasesJinanChina

Biomedical engineering is acclaimed as the most effective technological advancement-the stepping-stone toward better human *** in-depth look into biomedical engineering reveals that emerging materials are recognized as the turbocharger for its progress and the source of *** example,the application of advanced nanomaterials in drug delivery,bioimaging,gene therapy,and so on are pioneering new ways to diagnose and treat cancer and other diseases;tissue repair and organ regeneration are no longer dreams,but attainable miracles because of 3D bioprinted scaffold materials.

关键词： dreams claimed benefit

A Validated Rapid Stability-Indicating Method for the Determination of Related Substances in Vardenafil Hydrochloride by Ultra-Performance Liquid Chromatography

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American Journal of Analytical Chemistry 2012年第1期3卷 59-66页

作者： Kishore Kumar Kakumani Kameswara Rao Chimalakonda Rama Koti Reddy Yarram Mukkanti Khagga Centre for Chemical Science and Technology Institute of science and Technology Jawaharlal Nehru Technological University Hyderabad India Department of Analytical Research and Development Inogent Laboratories Pvt. Ltd. Hyderabad India Department of Instrumentation Engineering College of Engineering Andhra University Visakhapatnam India

A novel, sensitive, stability indicating RP-LC method has been developed for the quantitative determination of Varde- nafil and its related impurities in both bulk drugs and Pharmaceutical dosage forms. Effective chromatographic separation was achieved on a C18 stationary phase with simple mobile phase combination delivered in a simple gradient pro- gramme and quantitation was by ultraviolet detection at 210 nm. The mobile phase consisted of a buffer and acetonitrile delivered at a flow rate 0.25 ml?min–1. Buffer consisted of 20 mM Ammonium bi carbonate, pH adjusted to 5.0 by using ortho Phosphoric acid. In the developed UPLC method the resolution (Rs) between vardenafil and its four potential impurities was found to be grater than *** analysis showed an r value (correlation coefficient) grater than 0.999 for vardenafil and its four impurities. This method was capable to detect all four impurities of vardenafil at a level of 0.25 μ***–1 with respect to test concentration of 500 μg?ml–1 for a 2 μl injection volume. The inter and intra day precision values for all four impurities and for vardenafil was found to be with in 2.0% RSD. The method showed good and consistent recoveries for vardenafil in bulk drugs (98.8% - 100.9%), pharmaceutical dosage forms (100.5% - 101.5%) and its all four impurities (99.8% - 102.5%).The test solutions was found to stable in acetonitrile for 48 h. The drug was subjected to stress conditions of hydrolysis, oxidation, photolysis, and thermal degradation. Considerable degradation was found to occur in peroxide hydrolysis. The stress samples were assayed against a qualified reference standard and the mass balance was found close to 99.9%. The developed RP-LC method was validated with respect to linearity, accuracy, precision and robustness.

关键词： Vardenafil Validation Impurities and Degradation Products

Isolation of Bulk Amount of Piperine as Active Pharmaceutical Ingredient (API) from Black Pepper and White Pepper (<i>Piper nigrum</i>L.)

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Pharmacology & Pharmacy 2017年第7期8卷 253-262页

作者： Zihan Rahman Khan Fatema Moni Suriya Sharmin Muhammad Abdullah Al-Mansur Abdul Gafur Obaidur Rahman Farhana Afroz Pharmaceutical Sciences Research Division BCSIR Laboratories Dhaka Bangladesh Institute of National Analytical Research and Service (INARS) BCSIR Dhaka Bangladesh Pilot Plant & Process Development Center (PP & PDC) BCSIR Dhaka Bangladesh Department of Biology & Chemistry North South University Dhaka Bangladesh

In the pharmaceutical world the majority of the active pharmaceutical ingredients (API) have been obtained from the natural products. Piperine is such naturally occurring alkaloid which can be considered as major bioactive phytochemical having broad spectrum of pharmacological activities. It is obtained from the most valuable ethnomedicinal spices peppercorns i.e. black pepper and white pepper, which are the fruits of the Asian vine Piper nigrum L. Because of the widespread traditional uses of this medicinal compound, present article reveals a simple and effective isolation method of bulk piperine. The novelty of this investigation is to provide an idea for utilizing such natural method of large scale commercial piperine production as API drug in spite of chemical synthesis. Piperine was isolated in a pure crystal form and characterized by its melting point, X-Ray diffraction (XRD) studies and spectral data, including two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Chromatographic techniques like Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were applied to determine the purity of the yielded piperine. It was found that piperine yield from black pepper was within 2.5% - 3.0% and from white pepper within 4.0% - 4.5% and the purity of the yielded piperine was found to be up to 98.5% for black pepper and 98.2% for white pepper. Considering this yield value and purity it is indicated that, such effective isolation method can be successfully utilized for industrial large-scale production commercially. According to the result, it can be claimed that, as a natural product the isolated piperine can also be utilized as API drug like other expensive chemically synthesized piperine in different drug formulation.

关键词： Piper nigrum L NMR Spectroscopy X-Ray Diffraction Studies Bulk Piperine

drug repurposing for cancer therapy

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Signal Transduction and Targeted Therapy 2024年第5期9卷 1981-2013页

作者： Ying Xia Ming Sun Hai Huang Wei-Lin Jin Center for Clinical laboratories The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004PR China The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine Guiyang 550001PR China School of Clinical Laboratory Science Guizhou Medical UniversityGuiyang 550004PR China Division of Gastroenterology and Hepatology Department of Medicine andDepartment of OncologySidney Kimmel Comprehensive Cancer CenterJohns Hopkins University School of MedicineBaltimoreMD 21287USA Institute of Cancer Neuroscience Medical Frontier Innovation Research CenterThe First Hospital of Lanzhou UniversityThe First Clinical Medical College of Lanzhou UniversityLanzhou 730000PR China

Cancer,a complex and multifactorial disease,presents a significant challenge to global *** significant advances in surgical,radiotherapeutic and immunological approaches,which have improved cancer treatment outcomes,drug therapy continues to serve as a key therapeutic ***,the clinical efficacy of drug therapy is often constrained by drug resistance and severe toxic side effects,and thus there remains a critical need to develop novel cancer *** promising strategy that has received widespread attention in recent years is drug repurposing:the identification of new applications for existing,clinically approved *** repurposing possesses several inherent advantages in the context of cancer treatment since repurposed drugs are typically cost-effective,proven to be safe,and can significantly expedite the drug development process due to their already established safety *** light of this,the present review offers a comprehensive overview of the various methods employed in drug repurposing,specifically focusing on the repurposing of drugs to treat *** describe the antitumor properties of candidate drugs,and discuss in detail how they target both the hallmarks of cancer in tumor cells and the surrounding tumor *** addition,we examine the innovative strategy of integrating drug repurposing with nanotechnology to enhance topical drug *** also emphasize the critical role that repurposed drugs can play when used as part of a combination therapy *** conclude,we outline the challenges associated with repurposing drugs and consider the future prospects of these repurposed drugs transitioning into clinical application.

关键词： drugs integrating spite