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Photobiomodulation:a novel approach to promote trans-differentiation of adipose-derived stem cells into neuronal-like cells

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Neural Regeneration Research 2025年第2期20卷 598-608页

作者： Daniella Da Silva Madeleen Jansen van Rensburg Anine Crous Heidi Abrahamse Laser Research Centre Faculty of Health SciencesUniversity of JohannesburgDoornfonteinJohannesburgSouth Africa

Photobiomodulation,originally used red and near-infrared lasers,can alter cellular *** has been demonstrated that the visible spectrum at 451-540 nm does not necessarily increase cell proliferation,near-infrared light promotes adipose stem cell proliferation and affects adipose stem cell migration,which is necessary for the cells homing to the site of *** this in vitro study,we explored the potential of adipose-derived stem cells to differentiate into neurons for future translational regenerative treatments in neurodegenerative disorders and brain *** investigated the effects of various biological and chemical inducers on trans-differentiation and evaluated the impact of photobiomodulation using 825 nm near-infrared and 525 nm green laser light at 5 J/*** adipose-derived stem cells can be used in autologous grafting and photobiomodulation has been shown to have biostimulatory *** findings reveal that adipose-derived stem cells can indeed trans-differentiate into neuronal cells when exposed to inducers,with pre-induced cells exhibiting higher rates of proliferation and trans-differentiation compared with the control ***,green laser light stimulation led to notable morphological changes indicative of enhanced trans-differentiation,while near-infrared photobiomodulation notably increased the expression of neuronal *** biochemical analysis and enzyme-linked immunosorbent assays,we observed marked improvements in viability,proliferation,membrane permeability,and mitochondrial membrane potential,as well as increased protein levels of neuron-specific enolase and ciliary neurotrophic ***,our results demonstrate the efficacy of photobiomodulation in enhancing the trans-differentiation ability of adipose-derived stem cells,offering promising prospects for their use in regenerative medicine for neurodegenerative disorders and brain injuries.

关键词： differentiation inducers green photobiomodulation immortalized adipose-derived stem cell near-infrared photobiomodulation neurodegenerative disease neurogenesis photobiomodulation trans-differentiation

Spatial analysis of plant detritus processing in a Mediterranean River type: the case of the River Tirso Basin, Sardinia, Italy

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Journal of environmental sciences 2003年第2期15卷 227-240页

作者： Maurizio Pinna Franca Sangiorgio Alessio Fonnesu Alberto Basset Department of biological and environmental sciences and Technologies University of LecceDepartment of Biological and Environmental Sciences and Technologies University of LecceDepartment of Biological and Environmental Sciences and Technologies University of LecceDepartment of Biological and Environmental Sciences and Technologies University of Lecce 73100 Lecce Italy73100 Lecce Italy73100 Lecce Italy73100 Lecce Italy

The river continuum concept represents the most general framework addressing the spatial variation of both structure and function in river ecosystems. In the Mediterranean ecoregion, summer drought events and dams constitute the main sources of local disturbance to the structure and functioning of river ecosystems occurring in the river basin. In this study, we analysed patterns of spatial variation of detritus processing in a 7th order river of the Mediterranean ecoregion(River Tirso, Sardinia-Italy) and in three 4th order sub-basins which were exposed to different summer drought pressures. The study was carried out on Phragmites australis and Alnus glutinosa leaf detritus at 31 field sites in seasonal field experiment Detritus processing rates were higher for Alnus glutinosa than for Phragmites australis plant detritus. Processing rates of Alnus glutinosa leaves varied among seasons and study sites from 0.006 d -1 to 0.189 d -1 and those of Phragmites australis leaves ranged from 0.0008 d -1 to 0.102 d -1, with the lowest values occurring at sites exposed to summer drought. Seasons and sites accounted for a significant proportion of such variability. Alder detritus decay rates generally decreased with increasing stream order, while reed detritus decay rates generally increased on the same spatial gradient. Summer drought events affected these spatial patterns of variation by influencing significantly the decay rates of both plant detritus. The comparisons among and within sub-basins showed strong negative influence of summer drought on detritus processing rates. Similarly, in the entire River Tirso basin decay rates were always lower at disturbed than at undisturbed sites for each stream order; decay rates of reed detritus remained lower at those sites even after the end of the disturbance events, while alder decay rates recovered rapidly from the summer drought perturbations. The different recovery of the processing rates of the two leaves could also

关键词： ecosystem processes spatial patterns disturbance Mediterranean ecosystem type

Gene therapy for spinal muscular atrophy:perspectives on the possibility of optimizing SMN1 delivery to correct all neurological and systemic perturbations

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Neural Regeneration Research 2025年第7期20卷 2011-2012页

作者： Sharon J.Brown Rafael J.Yáñez-Muñoz Heidi R.Fuller School of Pharmacy and Bioengineering Keele UniversityKeeleUK Wolfson Center for Inherited Neuromuscular Disease TORCH BuildingRJAH Orthopaedic HospitalOswestryUK *** Center of Gene and Cell TherapyDepartment of Biological SciencesSchool of Life Sciences and the EnvironmentRoyal Holloway University of LondonEghamUK

Spinal muscular atrophy(SMA)is a genetic condition that results in selective lower motor neuron loss with concomitant muscle weakness and *** genetic cause of SMA was understood in 1995 when loss or impairment of the survival motor neuron 1(SMN1)gene was identified as the main contributing factor(Lefebvre et al.,1995).This,in combination with the discovery that humans have a“back-up”gene,SMN2,which can produce low levels(approximately 10%)of the full-length functional SMN protein,has led to the generation of SMA-specific gene *** was traditionally classified according to age of symptom onset and developmental milestones achieved,with life expectancy and severity varying between ***,SMN2 copy number is used as a proxy for the prediction of disease severity,with higher SMN2 copy number typically being associated with reduced severity of SMA,although this relationship is not absolute:some individuals with low SMN2 copy number have less severe SMA phenotypes and vice ***,the etiology of SMA is further complicated by other factors,such as non-typical nucleotide variants and SMN2-independent modifiers of disease severity.

关键词： atrophy traditionally absolute

Variation in the timing of river entry of Atlantic salmon (Salmo salar L.) in the Baltic

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Current Zoology 2009年第5期55卷 342-349页

作者： Anna KUPARINEN Juha MERIL Department of biological and environmental sciences

The timing of river entry in the Atlantic salmon is known to depend on genetic, demographic and environmental factors, but little is known about the relative magnitude of among population and among year variation and covariation in this respect in natural state Atlantic salmon rives. To investigate this, variability in the timing of river entry in three historical Finnish Atlantic salmon populations were analyzed using salmon trap data collected during 1870- 1902. The analyses reveled that 1 ） the timing of river entry differed substantially and consistently among the rivers, and that 2） variation among the rivers was much larger than variation among years. Annual variations were not explained by regional environmental conditions, whereas in one river the timing of the local flood peak was a significant predictor of the timing of river entry. Differences in the timing of salmon entry to geographically closely situated rivers suggests that a regionally fixed opening date for coastal fisheries might not be the best management strategy as it may lead to uneven exploitation of salmon populations from different rivers [ Current Zoology 55 （5）： 342 - 349, 2009] .

关键词： Atlantic salmon Coastal fisheries Fisheries management Freshwater entry Migration River entry

Impact of the COVID-19 pandemic on lung cancer treatment and research

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Genes & Diseases 2023年第4期10卷 1217-1219页

作者： Shreya Kailas Lovlekar Yihua Wang biological sciences Faculty of Environmental and Life SciencesUniversity of SouthamptonSouthampton SO171BJUK

Despite recent advances in immunotherapy and targeted therapy,the mortality rate seen in lung cancer(LC)is the highest of all cancer *** recent topical coronavirus disease 19(COVID-19)and its subsequent pandemic have resulted in over 505 million confirmed cases and approxi-mately 6.2 million fatalities as of April 2022,1 with a stag-gering 30%e50%mortality rate seen in LC patients with COVID-19.2 Cancer patients,in particular,are highly vulnerable to COVID-19 infection due to immunosuppression.

关键词： mortality cancer lung

Cell concentration,viability and culture composition of airborne bacteria during a dust event in Beijing

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Journal of environmental sciences 2017年第5期29卷 33-40页

作者： Hongli Yuan Daizhou Zhang Yanning Shi Baozhen Li Jinshui Yang Xuejian Yu Nan Chen Makiko Kakikawa State Key Laboratory of Agrobiotechnology College of Biological SciencesChina Agricultural University faculty of environmental and Symbiotic sciences Prefectural University of Kumamoto Institute of Nature and environmental Technology Kanazawa University

Airborne bacteria were measured when a dust storm passed Beijing in spring 2012 with a focus on cell concentration, viability and TSA- and R2A-cultured strain composition. The concentration varied at an order of 10^7 cells/m3 with dust loading （demonstrated with PM10） and they had a very close correlation （RT2 = 0.91, p 〈 0.01）. At the time of highest PM10 of 652 μg/m3, the bacterial concentration reached 1.4 × 10^8 cells/m3, which was larger than that before and after the dust event by one order. Bacterial viability, the ratio of number concentration of viable cells to total cells, was 32%-64% and smaller in the dust plume than that before the dust arrival. Bacterial strains from the culture ranged between 2.5 × 10^4 and 4.6 × 10^5 CFU/m3 and no correlation with PM10 was determined. Their composition was different before and after the dust arrival according to 16S rRNA gene sequences and strains belong to Actinomycetes and Firmicutes were the majority in the dust samples.

关键词： Bacteria Asian dust Beijing Fluorescence enumeration PM10

Soil-vegetation relationships in Mediterranean forests after fire

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Forest Ecosystems 2021年第2期8卷 240-252页

作者： Víctor Fernández-García Elena Marcos Sara Huerta Leonor Calvo Area of Ecology Department of Biodiversity and Environmental ManagementFaculty of Biological and Environmental SciencesUniversidad de León24071 LeónSpain

Background:Wildfires are one of the major environmental concerns in Mediterranean ***,many studies have addressed wildfire impacts on soil and vegetation in Mediterranean forests,but the linkages between these ecosystem compartments after fire are not well *** aim of this work is to analyze soil-vegetation relationships in Mediterranean burned forests as well as the consistency of these relationships among forests with different environmental conditions,at different times after fire,and among vegetation with different functional ***:Our results indicate that study site conditions play an important role in mediating soil-vegetation ***,we found that the nature of soil-vegetation relationships may vary over time as fire effects are less dominant in both ecosystem *** this,we detected several common soil-vegetation relationships among study sites and times after *** instance,our results revealed that available P content and stoichiometry(C:P and N:P)were closely linked to vegetation growth,and particularly to the growth of *** found that enzymatic activities and microbial biomass were inversely related to vegetation growth rates,whereas the specific activities of soil enzymes were higher in the areas with more vegetation height and ***,our results suggest that resprouters may influence soil properties more than seeders,the growth of seeders being more dependent on soil ***:We provide pioneer insights into how vegetation is influenced by soil,and vice-versa,in Mediterranean burned *** results reflect variability in soil-vegetation relationships among study sites and time after fire,but consistent patterns between soil properties and vegetation were also *** research is highly relevant to advance in forest science and could be useful to achieve efficient post-fire management.

关键词： Wildfires Soil nutrients Enzymatic activities Microbial biomass Growth forms Regenerative strategies

Cross-specific amplification of microsatellite DNA markers in Shorea platyclados

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Journal of Forestry Research 2016年第1期27卷 27-32页

作者： Asif J.Muhammad Charles H.Cannon Wickneswari Ratnam Department of biological sciences Texas Tech University faculty of Science and Technology School of Environmental and Natural Resource SciencesUniversiti Kebangsaan Malaysia

In the megadiverse forests of Southeast Asia, hundreds of timber species are economically important but the population genetics of only a few taxa are known. Cross-specific amplification of microsatellite loci among closely related taxa could enhance our ability to study and manage previously unstudied species. We successfully utilized STMS markers in Shorea platyclados, originally developed for Shorea curtisii. The six primer pairs we tried successfully produced PCR products of expected sizes. The number of alleles observed ranged from 10 to 14 and an average of 12 alleles were detected per locus. A high expected and observed heterozygosity was observed and it ranges from 0.718 to 0.827 among all populations across all six loci tested. Microsatellite DNA markers are highly polymorphic, co-dominant, reproducible, and amenable to high throughput genetic analyses. Overall, the cross- specific amplification of microsatellite loci appears to be complicated by numerous factors. While the approach may be effective for local management and conservation ofpoorly known species, the results must be carefully interpreted.

关键词： Genetic marker systems Populationgenetics SSR Selective logging Tropical timber Capillary electrophoresis

Assessment of nitrogen maintenance requirement in juvenile Black Porgy Acanthopagrus schlegeli

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Chinese Journal of Oceanology and Limnology 2009年第1期27卷 63-68页

作者：朱秋华钱国英高有领汪财生 Zhejiang Wanli University College of Biological and Environmental Sciences

Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM) and nitrogen maintenance requirement per unit metabolism body weight (NM’) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein (380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied from 0.15 to 0.31 mg/g BW per day, accounting for 12.2% to 21.1% of nitrogen intake. The amount of fecal nitrogen excretion varied from 0.21 to 0.32 mg/g BW per day, accounting for 16.3% to 21.6% of nitrogen intake. The endogenous nitrogen excretion, a main part of nitrogen consumption varied from 0.79 to 0.97 mg/g BW per day, accounting for 63.3 % to 68.0% of nitrogen intake. Positive correlation was found between NM and body weight, while a negative correlation was found between NM of unit body weight and the growth duration. No significant differences (p>0.05) were found in NM’ among different growth stages. The average of NM’ was 0.485 7mg/g per day. The results from nitrogen-free diet experiment showed that a negative correlation between NM and feed intake of nitrogen-free diet. NM increased with the decrease of feed intake of fish. The average of NM was 0.482 9 mg/g BW per day that was close to 0.483 8 mg/g BW obtained from fish with 120 g BW in nitrogen balance experiment. The nitrogen balance method is recommended to be a better method for determining NM in consideration of fish stress and result stability. This study also provides a calculated result of the protein content in diets, which is necessary for maintaining fish body protein at different growth stages. The calculation was based on the amount of nitrogen required for maintaining body protein per kg BW.

关键词： Acanthopagrus schlegeli nitrogen balance nitrogen-free diet nitrogen maintenancerequirement protein contain levels