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Functional characterization of the promoter of carbonyl reductase 1 gene in porcine endometrial cells

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第7期18卷 626-634页

作者： Ai-ling ZHANG Xian-yue SUN Qi YIN Jian-hua ZENG Zhe ZHANG Jia-qi LI Hao ZHANG Guangdong Development Center of Applied Ecology and ecological engineering in Universities Biology and Food Engineering Institute Guangdong University of Education Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding National Engineering Research Center for Breeding Swine Industry National & Local Joint Engineering Research Center for Livestock and Poultry Breeding College of Animal Science South China Agricultural University

Prostaglandins （PGs） play a critical role in porcine reproduction, of which prostaglandin E2 （PGE2） and prostaglandin F2a （PGF2a） exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enzyme, carbonyl reductase 1 （CBR1） catalyzes the conversion of PGE2 to PGF2a. A high ratio of PGE2：PGF2a is beneficial to the establishment and maintenance of porcine pregnancy. PG is essential for the establishment of pregnancy which resembles the proinflammatory response and nuclear factor KB （NF-KB） is involved in the process. Bioinformatic analysis has shown that NF-KB is a possible factor bound to two cis-regulatory elements in CBRI promoter. In this study, we cloned the 2997 bp （-2875/＋122） of the promoter, and constructed six 5＇-deleted dual-luciferase reporter recombinant vectors. In endometrial cells, the region of P2 （-16401＋7） exhibited the greatest transcriptional activity at driving luciferase expression, but not significantly different from that of P1 （-2089/＋7）. The activity of P1, P2, and P3 （-1019/＋7） was highly significantly higher than that of others （P〈0.01）, suggesting that two positive regulatory elements were likely present in the regions of -1640/-1019 and -1019/-647. The results also showed that the -1640/ -647 region was indispensable for the promoter. The results of chromatin immunoprecipitation （CHIP） demonstrated that the NF-KB subunit p65 binds to one site around -15451-1531. Using four reference genes, we found that the over-expression of p65 enhanced the expression of CBR1 （P〈0.05） in porcine endometrial epithelial cells, while knockdown of the p65 did not down-regulate the CBRI expression. These results indicated that NF-KB （p65） could bind to the special element of CBR1 gene promoter in porcine endometrial epithelial cells in vitro. The binding site of NF-KB was a positive regulator for the CBR1 gene promoter, but was not necessary for the basic expression.

关键词： Pig Carbonyl reductase 1 (CBR1) Promoter NF-KB Endometrium

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High-performance Mg–Zn alloy achieved by the ultrafine grain and nanoparticle design

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Bioactive Materials 2024年第11期41卷 371-384页

作者： Wenhui Wang Xiyue Zhang Anke Zhang Han Yu Xinbao Kang Cheng Wang Yang Song Jiahua Ni Mikhail L.Zheludkevich Xiaonong Zhang State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghaiChina College of biological Science and Medical engineering Donghua UniversityShanghaiChina The Second Affiliated Hospital Zhejiang UniversityZhejiangChina Institute of Surface Science Helmholtz-Zentrum HereonGeesthacht21502Germany Suzhou Origin Medical Technology Co.Ltd. JiangsuChina

Improving the comprehensive performance of low alloyed Mg is a significant challenge for biomedical *** paper developed a high-performance Mg–Zn alloy with uniform ultrafine grains and nano-precipitates through a straightforward,high-temperature reciprocating equal channel angle extrusion(ECAP)process and researched the microstructure,mechanical property,degradation behaviour,and biocompatibility of the developed *** showed that the lean Mg–2Zn alloy successfully refined grain to about 1μm and produced plenty of nano-particles with uniform distribution,providing high comprehensive mechanical properties(YS:235 MPa,UTS:267 MPa,EL:15.6%).Additionally,Zn-riched nano-particles in the matrix could decrease the Zn aggregation at the corrosion layer-matrix interface and form a dense oxide film,achieving a low degradation rate(0.13 mm/year in vivo).Finally,this work realizes the low alloy content,low cost,and good properties of one biodegradable Mg alloy,which will benefit the promotion of future clinical applications.

关键词： Biodegradable metal Mg alloy Ultrafine grains Nanoparticles ECAP

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Compositional engineering of HKUST-1/sulfidized NiMn-LDH on functionalized MWCNTs as remarkable bifunctional electrocatalysts for water splitting

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Carbon Energy 2023年第12期5卷 1-20页

作者： Mengshan Chen Reza Abazari Soheila Sanati Jing Chen Mingyuzhi Sun Cunhong Bai Alexander M.Kirillov Yingtang Zhou Guangzhi Hu Zhejiang Key Laboratory of Petrochemical Environmental Pollution Control National Engineering Research Center for Marine AquacultureMarine Science and Technology CollegeZhejiang Ocean UniversityZhoushanChina Department of Chemistry Faculty of ScienceUniversity of MaraghehMaraghehIran State Key Laboratory of High-Efficiency Utilization of Coal and Green Chemical engineering College of Chemistry and Chemical EngineeringNingxia UniversityYinchuanChina Departamento de Engenharia Química Centro de Química EstruturalInstitue of Molecular SciencesInstituto Superior TécnicoUniversidade de LisboaLisbonPortugal School of Ecology and Environmental Science Institute for Ecological Research and Pollution Control of Plateau LakesYunnan UniversityKunmingChina

Water-splitting reactions such as the hydrogen evolution reaction(HER)and the oxygen evolution reaction(OER)typically require expensive noble metal-based *** has motivated researchers to develop novel,cost-effective electrocatalytic *** this study,a new multicomponent nanocomposite was assembled by combining functionalized multiwalled carbon nanotubes,a Cu-based metal–organic framework(MOF)(HKUST-1 or HK),and a sulfidized NiMn-layered double hydroxide(NiMn-S).The resulting nanocomposite,abbreviated as MW/HK/NiMn-S,features a unique architecture,high porosity,numerous electroactive Cu/Ni/Mn sites,fast charge transfer,excellent structural stability,and *** a current density of 10 mA cm-2,this dual-function electrocatalyst shows remarkable performance,with ultralow overpotential values of 163 mV(OER)or 73 mV(HER),as well as low Tafel slopes(57 and 75 mV dec-1,respectively).Additionally,its high turnover frequency values(4.43 s-1 for OER;3.96 s-1 for HER)are significantly superior to those of standard noble metal-based Pt/C and IrO2 *** synergistic effect of the nanocomposite's different components is responsible for its enhanced electrocatalytic performance.A density functional theory study revealed that the multi-interface and multicomponent heterostructure contribute to increased electrical conductivity and decreased energy barrier,resulting in superior electrocatalytic HER/OER *** study presents a novel vision for designing advanced electrocatalysts with superior performance in water *** composites have been utilized in water-splitting *** study investigates the use of the MW/HK/NiMn-S electrocatalyst for water splitting for the first time to indicate the synergistic effect between carbon-based materials along with layered double hydroxide compounds and porous compounds of *** unique features of each component in this composite can be an interesting topic in the field of water spl

关键词： bifunctional electrocatalyst electroactive sites multicomponent synergic effect water splitting

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Real-time monitoring of optimum timing for harvesting fresh tea leaves based on machine vision

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International Journal of Agricultural and biological engineering 2019年第1期12卷 6-9页

作者： Liang Zhang Hongduo Zhang Yedong Chen Sihui Dai Xumeng Li Kenji Imou Zhonghua Liu Ming Li College of engineering Hunan Agricultural UniversityChangsha 410128China College of Horticulture and Landscape Hunan Agricultural UniversityChangsha 410128China Agricultural College Hunan Agricultural UniversityChangsha 410128China Department of biological and Environmental engineering Graduate School of Agricultural and Life SciencesUniversity of TokyoTokyo 113-0033Japan School of Agricultural engineering and Food Science Shandong University of TechnologyZibo 255000China Hunan Agricultural Aviation Advanced Technology engineering Research Center Changsha 410129China

The harvesting time of fresh tea leaves has a significant impact on product yield and *** aim of this study was to propose a method for real-time monitoring of the optimum harvesting time for picking fresh tea leaves based on machine ***,the shapes of fresh tea leaves were distinguished from RGB images of the tea-tree canopy after graying with the improved B-G algorithm,filtering with a median filter algorithm,binary processing with the Otsu algorithm,and noise reduction and edge smoothing using open and close *** the leaf characteristics,such as leaf area index,average length,and leaf identification index,were *** on these,the Bayesian discriminant principle and method were used to construct a discriminant model for fresh tea-leaf collection *** this method was applied to a RGB tea-tree canopy image acquired at 45°shooting angle,the fresh tea-leaf recognition rate was 90.3%,and the accuracy for fresh tea-leaf harvesting status was 98%by cross ***,this method provides the basic conditions for future tea-plantation operation and management using information technology,automation,and intelligent systems.

关键词： agricultural machinery fresh tea leaves machine vision intelligent recognition real-time monitoring

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Application of multiphoton microscopy in dermatological studies: A mini-review

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Journal of Innovative Optical Health Sciences 2014年第5期7卷 39-63页

作者： Eljah Yew Christopher R.owlands Peter T.C.So Singapore-MIT Alliance for Researchand Technology(SMART) 1 CREATE WayCREATE TowerSingapore 138602 Department of biological engineering Massachusetts Institute of Technology 77 Massachusetts Ave Cambridge MA 02139USA Department of Mechanical engineering Massachusetts In.stitute of Technology 77 Massachusetts Ave Cambridge MA 02139USA SGR Harrison Spectroscopy Laboratory 77 Massachusetts Ave Cambridge MA 02139USA

This review summarizes the historical and more recent developments of multiphoton microscopy,as applied to *** microscopy offers several advantages over competingmicroscopy techniques:there is an inherent axial sectioning,penetration depths that competewll with confocal microscopy on account of the use of near-infrared light,and many t wo-pho ton contrast mechanisms,such as second-harmonic generation,have no analogue in *** the penetration depths of photons into tissue are typically limited on the orderof hundreds of microns,this is of les concern in dermatology,as the skin is thin and *** a result,multiphoton microscopy in dermatology has generated a great deal ofmterest,much of which is summarized *** review covers the interaction of light and tissue,as well as the various considerations that must be made when designing an *** stateof multiphoton microscopy in imaging skin cancer and various other diseases is also discussed,along with the investigation of aging and regeneration phenomena,and finally,the use of mul-tiphoton microscopy to analyze the transdermal transport of drugs,cosmetics and other agents *** review concludes with a look at potential future research directions,especiallythose that are necessary to push these techniques into widespread clinical acceptance.

关键词： Multiphoton microscopy skin cancer,aging and regeneration.

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Synthesis of sponge-like TiO_(2) with surface-phase junctions for enhanced visible-light photocatalytic performance

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Chinese Chemical Letters 2021年第5期32卷 1823-1826页

作者： Yue Jiang Yao Qin Tianyu Yu Sijie Lin College of Environmental Science and engineering The Institute for Translational NanomedicineShanghai East HospitalTongji UniversityShanghai 200092China Key Laboratory of Yangtze River Water Environment Shanghai Institute of Pollution Control and Ecological SecurityTongji UniversityShanghai 200092China Institute for Regenerative Medicine The Institute for Translational NanomedicineShanghai East HospitalTongji University School of MedicineShanghai 200123China

Maximizing adsorption and catalytic active sites and promoting the photo-excited charge separation are two key factors to achieve excellent photocatalytic *** this study,we report a sol-gel synthesis approach to obtain non-metal doped TiO_(2)with sponge-like structure and surface-phase junctions all at *** doping of carbon and nitrogen shifted the activation wavelength to the visible-light region,the innovative use of perchloric acid as a pore-making agent led to the formation of three-dimensional lamellar and porous structure with surface-phase *** surface area with catalytic active sites rendered by the sponge-like structure and surface-phase junctions contributed to the much improved photocatalytic degradation efficiency toward rhodamine B,tetracycline and Disperse Red 60 with excellent reusability and *** improved gen eration and separati on efficie ncy of the photo-induced charge carriers of the as-prepared TiO_(2)were supported by electrochemical impedance measurements and transient photocurrent *** method could also be applied to other photocatalysts to achieve structural alteration and element doping simultaneously.

关键词： Structural alteration Surface-phase junction Photocatalytic degradation Visible light TiO_(2)

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Effects of soil amendment and foliar selenium applications on cadmium immobilization in soil and accumulation in wheat

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Pedosphere 2024年第6期34卷 1123-1135页

作者： Qiqi LIU Tianyi WU Qiugang WU Chen ZHANG Dafu WU Lihao ZHANG Jing ZHOU Huan ZHANG Jun ZHOU School of Resources and Environment Henan Institute of Science and Technology Key Laboratory of Soil Environment and Pollution Remediation Institute of Soil Science Chinese Academy of Sciences University of Chinese Academy of Sciences Jiangsu Design Institute of Geology for Mineral Resources National engineering and Technology Research Center for Red Soil Improvement Red Soil Ecological Experiment Station Chinese Academy of Sciences Sino-Japan Friendship Center for Environmental Protection

Wheat cadmium(Cd) contamination is one of the environmental and agricultural concerns worldwide. Here, the effects of applications of foliar selenium(Se) and soil amendments(lime, mixture of biofuel ash and sodium sulfide(DSO), and mixture of iron mine tailing and alkali lignin(MX)) on soil Cd fractions and wheat Cd accumulation were studied using laboratory incubation, greenhouse pot, and field plot experiments. In the laboratory incubation experiment, lime and MX applications significantly increased soil pH, DSO application significantly decreased soil p H, while all the three amendments significantly reduced soil bioavailable Cd fractions by 16%–21% compared to the control without any amendment. Foliar Se application significantly increased the growth and yield of the high-Cd-accumulating cultivar(Puxing5). In the greenhouse experiment, lime and MX applications considerably decreased grain Cd concentration by 55% and 47%, respectively, for Puxing5 and by 24% and 26%, respectively, for the low-Cd-accumulating cultivar(Zhoumai30). In the field experiment, grain Cd concentration was significantly reduced by 68% and 72% by lime and MX applications, respectively, for Puxing5. Application of DSO also significantly reduced grain Cd concentration by 58% for Puxing5 and 25% for Zhoumai30 in the greenhouse experiment, but increased grain Cd concentration by 22% for Puxing5 in the field experiment. Our results implicate that laboratory incubation and greenhouse experiment results are necessary to be verified in field, and foliar Se application is recommended when considering both financial costs and production safety.

关键词： bioavailable fraction field plot experiment incubation pot experiment soil sequential extraction

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Advances in greenhouse automation and controlled environment agriculture:A transition to plant factories and urban agriculture

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International Journal of Agricultural and biological engineering 2018年第1期11卷 1-22页

作者： Redmond Ramin Shamshiri Fatemeh Kalantari K.C.Ting Kelly R.Thorp Ibrahim A.Hameed Cornelia Weltzien Desa Ahmad Zahra Mojgan Shad Smart Farming Technology Research Center Department of Biological and Agricultural EngineeringFaculty of EngineeringUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Landscape Architecture Faculty of Design and ArchitectureUniversiti Putra Malaysia43400SerdangSelangorMalaysia Department of Agricultural and biological engineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA United States Department of Agriculture Agricultural Research ServiceU.S.Arid-Land Agricultural Research CenterMaricopaAZ 85138USA Dept.of ICT and Natural Sciences Faculty of Information Technology and Electrical EngineeringNorwegian University of Science and Technology(NTNU)Larsgårdsveien 2NO-6009ÅlesundNorway Leibniz Institute for Agricultural engineering and Bioeconomy Max-Eyth-Allee 10014469 Potsdam-BornimGermany Adaptive AgroTech Consultancy International CA 93955USA

Greenhouse cultivation has evolved from simple covered rows of open-fields crops to highly sophisticated controlled environment agriculture(CEA)facilities that projected the image of plant factories for urban *** advances and improvements in CEA have promoted the scientific solutions for the efficient production of plants in populated cities and multi-story *** deployment of CEA for urban agriculture requires many components and subsystems,as well as the understanding of the external influencing factors that should be systematically considered and *** review is an attempt to highlight some of the most recent advances in greenhouse technology and CEA in order to raise the awareness for technology transfer and adaptation,which is necessary for a successful transition to urban *** study reviewed several aspects of a high-tech CEA system including improvements in the frame and covering materials,environment perception and data sharing,and advanced microclimate control and energy optimization *** research highlighted urban agriculture and its derivatives,including vertical farming,rooftop greenhouses and plant factories which are the extensions of CEA and have emerged as a response to the growing population,environmental degradation,and urbanization that are threatening food ***,several opportunities and challenges have been identified in implementing the integrated CEA and vertical farming for urban agriculture.

关键词： smart agriculture greenhouse modelling urban agriculture vertical farming automation internet of things(IoT) wireless sensor network plant factories

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Core-satellite metal-organic framework@upconversion nanoparticle superstructures via electrostatic self-assembly for efficient photodynamic theranostics

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Nano Research 2020年第12期13卷 3377-3386页

作者： Zhike Li Xi Qiao Guihua He Xin Sun Danhua Feng Liefeng Hu Hua Xu Hai-Bing Xu Shengqian Ma Jian Tian Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Hubei Province Engineering and Technology Research Center for Fluorinated PharmaceuticalsSchool of Pharmaceutical SciencesWuhan UniversityWuhan430071China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei Collaborative Innovation Center for Advanced Organic Chemical MaterialsCollege of Chemistry and Chemical EngineeringHubei UniversityWuhan430062China Department of Chemistry University of North TexasDentonTX76201USA

The nanoplatforms based on upconversion nanoparticles(UCNPs)have shown great promise in amplified photodynamic therapy(PDT)triggered by near-infrared(NIR)***,their practical in vivo applications are hindered by the overheating effect of 980 nm excitation and low utilization of upconversion luminescence(UCL)by *** solve these defects,core-satellite metal-organic framework@UCNP superstructures,composed of a single metal-organic framework(MOF)NP as the core and Nd3+-sensitized UCNPs as the satellites,are designed and synthesized via a facile electrostatic self-assembly *** superstructures realize a high co-loading capacity of chlorin e6(Ce6)and rose bengal(RB)benefitted from the highly porous nature of MOF NPs,showing a strong spectral overlap between maximum absorption of photosensitizers and emission of *** in vitro and in vivo experiments demonstrate that the dual-photosensitizer superstructures have trimodal(magnetic resonance(MR)/UCL/fluorescence(FL))imaging functions and excellent antitumor effectiveness of PDT at 808 nm NIR light excitation,avoiding the laser irradiation-induced overheating *** study provides new insights for the development of highly efficient PDT nanodrugs toward precision theranostics.

关键词： core-satellite structures upconversion nanoparticles(UCNPs) nanoscale metal-organic frameworks photodynamic therapy theranostics

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Recent Development of Bio-inspired Porous Materials for Catalytic Applications

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Chemical Research in Chinese Universities 2023年第1期39卷 13-18页

作者： TANG Yongquan QI Ganggang WANG Sai MENG Xiangju XIAO Feng-Shou Department of Chemistry Zhejiang UniversityHangzhou310028P.R.China Stomatology Hospital School of StomatologyZhejiang UniversityHangzhou310006P.R.China Key Lab of Biomass Chemical engineering of Ministry of Education College of Chemical and Biological EngineeringZhejiang UniversityHangzhou310028P.R.China Department of Chemistry Zhengzhou UniversityZhengzhou450001P.R.China

Nature has served as a source of inspiration for chemists to design the catalysts with superior performance via mimicking their characteristics,such as active sites,reaction microenvironment and electron transfer *** a few decades,many different types of materials have been widely used to construct the biomimetic *** these materials,the porous materials have been demonstrated to be powerful platforms for constructing the biomimetic catalysts owing to their high surface area,controllable composition and easy *** this review,we briefly showed recent advances in the construction of various biomimetic porous catalysts using porous materials for the design of excellent *** addition,we also present the challenge and opportunities in this emerging field.

关键词： Bio-inspired Porous material Biomimetic catalyst

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