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Metabolic relationships between marine red algae and algae‑associated bacteria

Marine Life science & Technology 2024年第2期6卷 298-314页

作者： Kyung Hyun Kim Jeong Min Kim Ju Hye Baek Sang Eun Jeong Hocheol Kim Hwan Su Yoon Che Ok Jeon Department of biological sciences and Biotechnology Hannam UniversityDaejon 34054Republic of Korea Department of Life science Chung-Ang UniversitySeoul 06974Republic of Korea Department of biological sciences Sungkyunkwan UniversitySuwon 16419Republic of Korea

Mutualistic interactions between marine phototrophs and associated bacteria are an important strategy for their success-ful survival in the ocean,but little is known about their metabolic ***,bacterial communities in the algal sphere(AS)and bulk solution(BS)of nine marine red algal cultures were analyzed,and Roseibium and Phycisphaera were identified significantly more abundantly in AS than in *** metabolic features of Roseibium RMAR6-6(isolated and genome-sequenced),Phycisphaera MAG 12(obtained by metagenomic sequencing),and a marine red alga,Porphyridium purpureum CCMP1328(from GenBank),were analyzed ***6-6 has the genetic capability to fix nitrogen and produce B vitamins(B1,B2,B5,B6,B9,and B12),bacterioferritin,dimethylsulfoniopropionate(DMSP),and phenylacetate that may enhance algal growth,whereas MAG 12 may have a limited metabolic capability,not producing vitamins B9 and B12,DMSP,phenylacetate,and siderophores,but with the ability to produce bacitracin,possibly modulating algal *** CCMP1328 lacks the genetic capability to fix nitrogen and produce vitamin B12,DMSP,phenylacetate,and *** was shown that the nitrogen-fixing ability of RMAR6-6 promoted the growth of ***-pureum,and DMSP reduced the oxidative stress of *** metabolic interactions between strain RMAR6-6 and *** CCMP1328 were also investigated by the transcriptomic analyses of their monoculture and *** together,potential metabolic relationships between Roseibium and *** were *** study provides a bet-ter understanding of the metabolic relationships between marine algae and algae-associated bacteria for successful growth.

关键词： Metabolic relationship Marine red algae Symbiotic bacteria Roseibium Phycisphaera Porphyridium

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Navigating biomolecular condensates in plants from patterns to functions

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Molecular Plant 2024年第9期17卷 1329-1332页

作者： Yansong Miao Monika Chodasiewicz Xiaofeng Fang School of biological sciences Nanyang Technological UniversitySingapore 637551Singapore institute for Digital Molecular Analytics and science Nanyang Technological UniversitySingapore636921Singapore biological and Environmental science&Engineering Division(BESE) King Abdullah University of Science and Technology(KAUST)ThuwalSaudi Arabia School of Life sciences Tsinghua UniversityBeijingChina

Biomolecular condensates have emerged as one of the important focuses of recent research on plant *** study of these condensates often begins with evidence gathered from imaging or bioinformatics *** with genetic and biochemical approaches,researchers have begun to establish a link between the condensation of biomolecules *** challenge,however,is unambiguously demonstrating the necessity of condensation in a cellular process versus the macromolecule *** requires a combination of evidence from genetics,cell biology,biochemistry,and biophysical *** this Opinion paper,we pinpoint the factors to be considered when studying the role of biomolecular condensates in plant *** also provide future directions for plant condensate biology.

关键词： functions. condensation directions

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Highly efficient expression of human extracellular superoxide dismutase(rhEcSOD)with ultraviolet-B-induced damage-resistance activity in transgenic silkworm cocoons

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Insect science 2024年第4期31卷 1150-1164页

作者： Feng Wang Ri-Yuan Wang De-Bin Zhong Ping Zhao Qing-You Xia Integrative science Center of Germplasm Creation in Western China(CHONGQING)science City Biological Science Research CenterSouthwest UniversityChongqingChina Century Legend Biotechnology Research Institute(Chongqing)Co. Ltd.ChongqingChina

Extracellular superoxide dismutase(EcSOD)protects tissues from oxidative stress,and thus is considered as a therapeutic agent for many diseases such as atherosclerosis,hypertension,and ***,cost-effective production of bioactive recombinant human EcSOD(rhEcSOD)remains a ***,we developed an efficient strategy for producing active rhEcSOD by transgenic *** was successfully synthesized as homodimers and homotetramers in the middle silk gland and spun into the cocoons with a concentration of 9.48±0.21 mg/*** of rhEcSOD from the cocoons could be conveniently achieved with a purity of 99.50%and a yield of 3.5±0.5 mg/***,N-glycosylation at the only site of N89 in rhEcSOD with 10 types were *** purified rhEcSOD gained the potent enzymatic activity of 4162±293 U/mg after Cu/Zn ions *** importantly,rhEcSOD was capable of penetrating and accumulating in the nuclei of cells to maintain cell morphology and attenuate ultraviolet B-induced cell apoptosis by eliminating reactive oxygen species and inhibiting the C-Jun N-terminal kinase signaling *** results demonstrated that the transgenic silkworm could successfully produce rhEcSOD with enzymatic and biological activities for biomedical applications.

关键词： antioxidant human extracellular superoxide dismutase recombinant expression silkworm silk gland

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Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo

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Food science and Human Wellness 2024年第5期13卷 2601-2616页

作者： Xiaoli Li Runhui Ma Zhijing Ni Wei Wang Kiran Thakur Jianguo Zhang Zhaojun Wei School of biological science and Engineering North Minzu UniversityYinchuan 750021China School of Food and biological Engineering Hefei University of TechnologyHefei 230009China School of Public Health Anhui University of Science and TechnologyHuainan 232001China

With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma(EC), we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization. The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth. The high-throughput sequencing data indicated that dioscin not only promoted apoptosis, including decrease of poly ADP-ribose polymerase(PARP) and B-cell lymphoma-2(Bcl-2) and increase of c-PARP and Bcl-2-associcated agonist of cell death(Bad), but also induced autophagy, including increase of autophagic lysosomes and LC3Ⅱ/LC3Ⅰ ratio. Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway. Besides, the dioscin-regulated p53 pathway was mainly involved in autophagy induction. Furthermore, inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis. Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

关键词： Dioscin Ishikawa cell Apoptosis Autophagy PI3K/AKT p53

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Proteomics Study of Benzene Metabolite Hydroquinone Induced Hematotoxicity in K562 Cells

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Biomedical and Environmental sciences 2024年第4期37卷 341-353页

作者： JIN Yi Shan YI Zong Chun ZHANG Yu Jing RONG Long YU Chun Hong School of biological science and Medical Engineering Beihang UniversityBeijing 100191China School of Engineering Medicine Beihang UniversityBeijing 100191China

Objective Hydroquinone(HQ),one of the phenolic metabolites of benzene,is widely recognized as an important participant in benzene-induced ***,there are few relevant proteomics in HQ-induced hematotoxicity and the mechanism hasn’t been fully understood *** In this study,we treated K562 cells with 40μmol/L HQ for 72 h,examined and validated protein expression changes by Label-free proteomic analysis and Parallel reaction monitoring(PRM),and performed bioinformatics analysis to identify interaction *** One hundred and eighty-seven upregulated differentially expressed proteins(DEPs)and 279 downregulated DEPs were identified in HQ-exposed K562 cells,which were involved in neutrophilmediated immunity,blood microparticle,and other GO terms,as well as the lysosome,metabolic,cell cycle,and cellular senescence-related *** on the 23 DEGs and 5 DEPs in erythroid differentiation-related pathways,we constructed the network of protein interactions and determined 6 DEPs(STAT1,STAT3,CASP3,KIT,STAT5B,and VEGFA)as main hub proteins with the most interactions,among which STATs made a central impact and may be potential biomarkers of HQ-induced *** Our work reinforced the use of proteomics and bioinformatic approaches to advance knowledge on molecular mechanisms of HQ-induced hematotoxicity at the protein level and provide a valuable basis for further clarification.

关键词： Hydroquinone Proteomics Hematotoxicity K562 cells

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Genome-wide CRISPR screening reveals key genes and pathways associated with 20-hydroxyecdysone signal transduction in the silkworm(Bombyx mori)

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Insect science 2024年第1期31卷 47-58页

作者： Hao Sun Jingya Chen Ruolin Wang Dan Liu Na Zhang Tong Zhang Ling Jia Sanyuan Ma Qingyou Xia State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqingChina biological science Research Center Southwest UniversityChongqingChina

Metamorphosis is a complex developmental process involving multiple pathways and a large number of genes that are regulated by juvenile hormone(JH)and 20-hydroxyecdysone(20E).Despite important progress in understanding various aspects of silkworm biology,the hormone signaling pathway in the silkworm remains poorly ***-wide screening using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)-based libraries has recently emerged as a novel method for analyzing genome function,enabling further research into essential genes,drug targets,and virus-host ***,we constructed a genome-wide CRISPR/Cas9-based library of the silkworm(Bombyx mori)and successfully revealed the genes involved in biotic or abiotic stress factor *** this study,we used our silkworm CRISPR library and large-scale genome-wide screening to analyze the key genes in the silkworm 20E signaling pathway and their mechanisms of *** annotation showed that 20E regulates key proteins in processes that mainly occur in the cytoplasm and *** enrichment analysis showed that 20E can activate phosphorylation and may affect innate immunity,interfere with intracellular nutrition and energy metabolism,and eventually cause cell *** screening results were experimentally validated by generating cells with knockout alleles of the relevant genes,which had increased tolerance to *** findings provide a panoramic overview of signaling in response to 20E in the silkworm,underscoring the utility of genome-wide CRISPR mutant libraries in deciphering hormone signaling pathways and the mechanisms that regulate metamorphosis in insects.

关键词： CRISPR/Cas9 CRISPR library screening ecdysone 20E signal transduction silkworm whole genome editing

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Systems science in the new era: intelligent systems and big data

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science China(Information sciences) 2024年第3期67卷 308-310页

作者： Wenwu YU Duxin CHEN Hongzhe LIU He WANG Jinde CAO Zengru DI Xiaojun DUAN Bin JIA Xiaodong DING Yiguang HONG School of Mathematics Southeast University Purple Mountain laboratories International Academic Center of Complex Systems Beijing Normal University College of science National University of Defense Technology School of Systems science Beijing Jiaotong University Business School University of Shanghai for Science and Technology Department of Control science and Engineering Tongji University

This study introduces the emergence, research challenges,and future development of intelligent systems and big data(ISBD) and discusses the significance of ISBD to systems science. To this end, an overview of systems science is first presented, followed by the conclusion that the development of artificial intelligence(AI) has promoted the derivation and development of intelligent systems(ISs).

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The endogenous antioxidant ability of royal jelly in Drosophila is independent of Keap1/Nrf2 by activating oxidoreductase activity

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Insect science 2024年第2期31卷 503-523页

作者： Dongjing Wen Jiayu Xie Yao Yuan Lirong Shen Yufeng Yang Wenfeng Chen Institute of Life sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouChina School of Medicine Chongqing UniversityChongqingChina College of Biosystems Engineering and Food science Zhejiang UniversityHangzhouChina

Royal jelly(RJ)is a biologically active substance secreted by the hypopharyngeal and mandibular glands of worker *** is widely claimed that RJ reduces oxidative ***,the antioxidant activity of RJ has mostly been determined by in vitro chemical detection methods or by external administration drugs that cause oxidative *** RJ can clear the endogenous production of reactive oxygen species(ROS)in cells remains largely ***,we systematically investigated the antioxidant properties of RJ using several endogenous oxidative stress models of *** found that RJ enhanced sleep quality of aging Drosophila,which is decreased due to an increase of oxidative damage with *** supplementation improved survival and suppressed ROS levels in gut cells of flies upon exposure to hydrogen peroxide or to the neurotoxic agent ***,RJ supplementation moderated levels of ROS in endogenous gut cells and extended lifespan after exposure of flies to heat *** deprivation leads to accumulation of ROS in the gut cells,and RJ attenuated the consequences of oxidative stress caused by sleep loss and prolonged ***,RJ prevented cell oxidative damage caused by heat stress or sleep deprivation,with the antioxidant activity in vivo independent of Keap1/Nrf2 *** supplementation activated oxidoreductase activity in the guts of flies,suggesting its ability to inhibit endogenous oxidative stress and maintain health,possibly in humans.

关键词： antioxidant biological oxidations Drosophila oxidative stress royal jelly sleep loss

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Conversion-type anode chemistry with interfacial compatibility toward Ah-level near-neutral high-voltage zinc ion batteries

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National science Review 2024年第7期11卷 226-238页

作者： Shan Guo Liping Qin Jia Wu Zhexuan Liu Yuhao Huang Yiman Xie Guozhao Fang Shuquan Liang School of Materials science and Engineering Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan ProvinceCentral South University College of biological and Chemical Engineering Guangxi University of Science and Technology Information and Network Center Central South University

High-voltage aqueous zinc ion batteries（AZIBs） with a high-safety near-neutral electrolyte is of great significance for practical sustainable application; however, they suffer from anode and electrode/electrolyte interfacial incompatibility. Herein, a conversion-type anode chemistry with a low anodic potential, which is guided by the Gibbs free energy change of conversion reaction, was designed for high-voltage near-neutral AZIBs. A reversible conversion reaction between ZnC2O4·2H2O particles and three-dimensional Zn metal networks well-matched in CH3COOLi-based electrolyte was revealed. This mechanism can be universally validated in the battery systems with sodium or iodine ions. More importantly, a cathodic crowded micellar electrolyte with a water confinement effect was proposed in which lies the core for the stability and reversibility of the cathode under an operating platform voltage beyond 2.0 V, obtaining a capacity retention of 95% after 100 cycles. Remarkably, the scientific and technological challenges from the coin cell to Ah-scale battery, sluggish kinetics of the solid-solid electrode reaction, capacity excitation under high loading of active material, and preparation complexities associated with large-area quasi-solid electrolytes,were explored, successfully achieving an 88% capacity retention under high loading of more than 20 mg cm-2and particularly a practical 1.1 Ah-level pouch cell. This work provides a path for designing low-cost, eco-friendly and high-voltage aqueous batteries.

关键词： zinc compound anode conversion reaction voltage regulation interfacial design pouch cell

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Advances in functional coatings on biliary stents

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Regenerative Biomaterials 2024年第2期3卷 55-75页

作者： Kaining Yang Wenxin Sun Lanyue Cui Yuhong Zou Cuie Wen Rongchang Zeng Department of Bioengineering College of Chemical and Biological EngineeringShandong University of Science and TechnologyQingdao 266590China Corrosion Laboratory for Light Metals College of Materials Science and EngineeringShandong University of Science and TechnologyQingdao 266590China school of Engineering RMIT UniversityMelbourneVIC3001Australia

Biliary stenting is an important interventional method for the prevention and treatment of biliary tract ***,complications,such as postoperative biliary infection and re-stenosis,frequently occur due to the extensive scope of the biliary system and the complex composition of *** com-bination of coating technology and biliary stents is expected to bring new approaches to the solution of these *** cutting-edge advance on functional coatings on biliary stents is reviewed from seven perspectives:anticorrosion,-bacterial,-tumor,stone-dissolving,X-ray visibility,anti-stent migration and functional composite *** de-velopment trend is also *** performance of the numerous functional coatings for various purposes is generally up to expectations,but the balance between the medica tions'effectiveness and their safety needs to be further *** contemporary investigations have advanced to the leve of animal experiments,offering crucial fundamental assurance for broader human *** combination of biliary stent and functional coatings is an innovative idea with great potential for future development.

关键词： biliary stent coating anticorrosion antibacterial antitumor stone-dissolving X-ray visibility antistent migration

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