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HPLC-DAD analysis and antifungal effect of Hyptis martiusii Benth(Lamiaceae) against Candida strains

Asian Pacific Journal of Tropical Biomedicine 2019年第3期9卷 123-128页

作者： Janaína Esmeraldo Rocha Henrique Douglas Melo Coutinho Cícero Roberto Nascimento Saraiva Antonia Thassya Lucas dos Santos Ant?nio Judson Targino Machado Jo?o Tavares Calixto Júnior Irwin Rose Alencar Menezes José Galberto Martins da Costa Jaime Ribeiro-Filho Aracelio Viana Colares laboratory of Microbiology and Biology Molecular Department of Biological Chemistry Regional University of Cariri Leao Sampaio University Center Department of biological Sciences Regional University of Cariri laboratory of Pharmacology and Molecular Chemistry Department of Biological Chemistry Regional University of Cariri laboratory of Natural product Research Department of Biological Chemistry Regional University of Cariri

Objective: To evaluate the anti Candida activity of Hyptis martiusii decoction and its major compound, caffeic acid alone or in the presence of fluconazole, as well as their cytotoxic effect. Methods: The decoction was characterized using high performance liquid chromatography coupled with diode array detector. For the antifungal activity, the minimum inhibitory concentration(MIC) and the potential effect of the decoction with the fluconazole were evaluated by microdilution method using 96-well microtiter trays. The osmotic fragility test was performed using erythrocytes under saline stress. All tests were performed in triplicate. Results: The chemical characterization of the decoction was performed by high performance liquid chromatography and revealed the presence of seven compounds, including caffeic acid as major constituent. The antifungal tests demonstrated that both decoction(DHm) and caffeic acid obtained from Hyptis martiusii presented MIC and MFC ≥4096 μg/mL against Candida albicans and Candida tropicalis strains. However, in the presence of fluconazole, DHm and caffeic acid presented IC_(50) of 2.60 and 2.53 μg/mL respectively, demonstrating significant synergistic effects against Candida strains. The modulator activity of DHm might be due to the presence of caffeic acid. Moreover, DHm and caffeic acid did not cause significant hemolytic effects, indicating that they present low cytotoxicity. Conclusions: These data indicate that DHm potentiates the activity of the fluconazole, without enhancement of the toxicity, encouraging further toxicological, pharmacological and phytochemical studies to provide consistent evidence of the potential of this plant to be used in drug development.

关键词： Hyptis martiusii Caffeic acid Candida albicans Candida tropicalis Antifungal properties

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Penicillium and patulin distribution in pears contaminated with Penicillium expansum. Determination of patulin in pears by UHPLC-MS/MS

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Journal of Integrative Agriculture 2017年第7期16卷 1645-1651页

作者： WEI Dong-mei XU Jun DONG Feng-shou LIU Xin-gang WU Xiao-hu ZHENG Yong-quan Risk Assessment laboratory for biological Hazards of Agricultural product Quality and Safety Ministry of Agriculture/Institute of Plant Protection Chinese Academy of Agricultural Sciences College of Plant Protection Shenyang Agricultural University

The danger of mycotoxin contamination entering the food supply through post-harvest infection is of perennial concern to food safety experts. To explore the distribution of Penicillium expansum and diffusion of its mycotoxin, patulin, in blue mold-damaged pears, Pyrus bretschneideri Rehd. cv. Yali obtained from markets and orchards in China were artificially inoculated with P. expansum and assayed for patulin accumulation and degree of fungal colonization. The inoculated pears were incubated until the lesions were 5, 10, 20, or 30 mm in diameter. We sampled tissue at a range of distances from the lesion, measured the spread of Penicillium by plate colony-counting methods, and used UHPLC-MS/MS to detect and quantify the patulin concentration. More P. expansum colony-forming units were isolated from pears with a higher degree of decay. Farther from the lesion, the fewer P. expansum colonies were observed, and the lower the patulin content detected. We found a significant difference in the patulin content between samples due to lesion size, and also in tissue sampled 10 mm away from the lesion. In consideration of this finding, to ensure food safety, we recommend that when a blue mold rot lesion on pear is 5, 10, or 20 mm in diameter, 20, 30, and 40 mm beyond the lesion should be removed, respectively. If a lesion surpasses 30 mm in diameter, the whole pear should be thrown away.

关键词： pear Penicillium expansum patulin food safety UHPLC-MS/MS

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The role of flavonoids in mitigating food originated heterocyclic aromatic amines that concerns human wellness

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Food Science and Human Wellness 2023年第4期12卷 975-985页

作者： Hui Teng Hongting Deng Chang Zhang Hui Cao Qun Huang Lei Chen College of Food Science and Technology Guangdong Ocean UniversityGuangdong Provincial Key Laboratory of Aquatic Product Processing and SafetyGuangdong Province Engineering Laboratory for Marine Biological ProductsGuangdong Provincial Engineering Technology Research Center of SeafoodKey Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education InstitutionZhanjiang 524088China Faculty of Food Science and Technology University of VigoVigo 36310Spain Meat Processing Key laboratory of Sichuan Province School of Food and Biological EngineeringChengdu UniversityChengdu 610106China

Meat products are an important part in our daily diet,providing valuable nutrients for the human ***,heating processes cause the meat to become more appetizing with changes in texture,appearance,flavor,and chemical properties by the altering of protein structure and other *** one kind of cooking-induced contaminants,heterocyclic aromatic amines(HAAs)are widely present in protein aceous food products with strong carcinogenic and mutagenic *** order to promote the safety of traditional meat products,this review focused on the formation,metabolism,biological monitoring and inhibitory mechanism of *** overview of the formation pathways,hazards,and control methods of HAAs during food processing in recent years was studied,aiming to provide some valuable information for exploring effective methods to inhibit the production of associated hazards during food *** selection of different types of flavonoids to explore their effects on the formation of HAAs in an actual barbecue system can provide theoretical reference for effectively controlling the formation of HAAs and reducing their harm to human health.

关键词： Heterocyclic aromatic amines Flavonoids Hazardous control Precursors

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Physicochemical properties of gelatin produced from Nile tilapia skin using chemical and fermentation pretreatments

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Food Bioscience 2022年第3期47卷 565-571页

作者： Tingju Chen Zengliu Song Huanming Liu Chunxia Zhou Pengzhi Hong Chujin Deng Guangdong Provincial Key laboratory of Aquatic product Processing and Safety Guangdong Province Engineering Laboratory for Marine Biological ProductsGuangdong Provincial Engineering Technology Research Center of Marine FoodKey Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education InstitutionGuangdong Modern Agricultural Science and Technology Innovation CenterCollege of Food Science and TechnologyGuangdong Ocean UniversityZhanjiang524088China

In this study,we aimed to verify the effectiveness of fermentation as a pretreatment in Nile tilapia(Oreochromis niloticus)skin gelatin production and to assess its effects on the extracted ***-gelatin(CG)and fermentation-gelatin(FG)were extracted using hot water from tilapia skin after immersion in chemical reagents(sodium hydroxide and butanol)and after fermentation with Bacillus velezensis FEL-BM21,*** presented a higher recovery(27.40%),yield(49.99%),and imino acid(proline and hydroxyproline)content(177.17±0.35 residues/1000 residues)than CG,but had a lower molecular *** transform infrared and circular dichroism spectroscopy confirmed that the collagen was denatured,and scanning electron microscopy revealed that FG had a more continuous filamentous structure than ***,except for transmittance(66.19%at 450 nm and 86.36%at 620 nm),FG exhibited higher gel strength(492.81 g),gelling point(28.93℃),and melting point(18.33℃)than *** findings suggest that fermentation can be used to treat gelatin raw materials without negatively affecting the structure of ***,fermentation has potential industrial applications as an alternative to chemical treatment.

关键词： Gelatin Nile tilapia Oreochromis niloticus Fermentation Pretreatment

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Nutritional properties of Europen eel(Anguilla anguilla)bone peptide-calcium and its apoptosis effect on Caco-2 cells

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Food Science and Human Wellness 2022年第6期11卷 1482-1490页

作者： Hui Teng Yuewei Qian Xiaoyun Fan Hui Cao Yuting Tian Lei Chen Guangdong Provincial Key laboratory of Aquatic product Processing and Safety Guangdong Province Engineering Laboratory for Marine Biological ProductsGuangdong Provincial Engineering Technology Research Center of SeafoodKey Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education InstitutionCollege of Food Science and TechnologyGuangdong Ocean UniversityZhanjiang 524088China College of Food Science Fujian Agriculture and Forestry UniversityFuzhou 350002China

The present study aimed at making a rational usage for European eel bone by-products by preparing Europen eel bone peptide chelated calcium(EBPC-Ca).Nutritional properties and bioactivity of EBPC-Ca were *** showed that nutritional properties of calcium ions will cause intra-and inter-molecular folding and aggregation of peptide to uniformly form EBPC-Ca *** chelated compound of EBPC and calcium ion triggered a strong apoptosis in heterogeneous human epithelial colorectal adenocarcinoma(Caco-2)in concentration-and time-dependent *** blot analysis revealed that the EBPC-Ca induced apoptosis may be the result of a blocked autophagy flux through mitochondrial-dependent ***,the increase in FGF-23 protein expression inhibited the absorption of calcium ions and alleviated cell *** was also found that the cell apoptosis occurs with significant increases in the levels of reactive oxygen species(ROS)and Ca^(2+)in the cells,indicating the anti-tumor potential of EBPC-Ca may involve multiple channels.

关键词： Europen eel bone Peptide Calcium Nutritional properties Caco-2 Apoptosis

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Assessing the product quality and biological activities of barley(Hordeum vulgare L.)grasses at different harvest times

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Food Bioscience 2022年第2期46卷 468-476页

作者： Jing-Kun Yan Ting-Ting Chen Xu Chen Yujia Liu Chenghong Liu Lin Li Key laboratory of Healthy Food Development and Nutrition Regulation of China National Light Industry School of Chemical Engineering and Energy TechnologyDongguan University of TechnologyDongguan523808China School of Food&biological Engineering Jiangsu UniversityZhenjiang212013China Biotech Research Institute Shanghai Academy of Agricultural Sciences Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai201106China

Barley(Hordeum vulgare L.)grasses(BG)have attracted considerable interest due to their multiple physiological activities and health *** this paper,eight BG at four different growth stages were collected:seedling,tillering,stem elongation,and heading,and their product qualities and biological activities were examined and *** demonstrated that harvest time obviously influenced proximate composition,surface color,and amino acid profile.Z21 and Z23 harvested at the seedling and early tillering stages,respectively,had higher total phenolic content(TPC)and total flavonoid content(TFC)than the other ***,Z21 and Z23 possessed better DPPH radical-scavenging ability,antioxidant capacity,andα-glucosidase inhibitory activity,which were positively correlated with TPC,TFC,and protein and total amino acid *** findings suggested that the early tillering stages were the preferable harvest times for BG with good product quality and biological properties.

关键词： Hordeum vulgare L. Barley grass Harvest time Phytochemicals product quality biological activity

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Targeted inhibition NCOA4-mediated ferritinophagy limits ferroptosis to ameliorate alcohol-induced liver injury in vivo and in vitro

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Food Science and Human Wellness 2024年

作者： Yanan Zhao Bin Li Jiang Liu Fei Yu Lei Chen Hui Teng College of Food Science and Technology， Guangdong Ocean University Guangdong Provincial Key laboratory of Aquatic product Processing and Safety Guangdong Province Engineering laboratory for Marine biological products Guangdong Provincial Engineering Technology Research Center of Seafood Key laboratory of Advanced Processing of Aquatic product of Guangdong Higher Education Institution

Alcoholic liver injury (ALD) is a serious liver disease resulting from prolonged and excessive alcohol consumption， with significant morbidity and mortality and closely relate to iron accumulation and ferroptosis. Ferritinophagy is the process of autophagic degradation of ferritin and results in labile iron accumulation and ferroptosis. However， whether ferritinophagy involves in alcohol-induced liver injury and ferroptosis remains unexplored. Here， we demonstrated the involvement of ferroptosis in alcohol-induced liver injury by modulating nuclear receptor coactivator 4 (NCOA4)-mediated ferritinophagy. Utilizing both in vivo and in vitro models， we observed activation of autophagy and ferroptosis following alcohol administration. Intriguingly， we found that alcohol-induced ferroptosis is autophagy-dependent， because autophagy inhibitor 3-MA (30 mg/kg bw. in vivo， 1 mM in vitro， respectively) profoundly mitigated alcohol-induced ferroptosis. We further demonstrated that alcohol-induced ferritinophagy was derived by iron overload， by showing that iron chelator DFO (100 mg/kg bw. in vivo， 10 μM in vitro， respectively) can abolish alcohol-induced ferritinophagy and ferroptosis. Moreover， we observed an upregulation of NCOA4 expression， and knockdown of NCOA4 significantly reversed alcohol-induced disturbances in iron metabolism and subsequently blocking ferroptosis. Our findings suggest a connection between ferritinophagy and alcohol-induced ferroptosis in mouse liver and HepG2 cells， highlighting NCOA4-mediated ferritinophagy as a novel mechanism of alcohol-induced liver injury. This study enhances our understanding of the molecular mechanisms underlying alcohol-induced liver injury， and targeting ferritinophagy may provide new strategies for preventing or treating ALD.

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The pyruvate dehydrogenase kinase 2(PDK2)is associated with conidiation,mycelial growth,and pathogenicity in Fusarium graminearum

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Food production, Processing and Nutrition 2020年第1期2卷 102-111页

作者： Tao Gao Dan He Xin Liu Fang Ji Jianhong Xu Jianrong Shi Jiangsu Key laboratory for Food Quality and Safety-State Key laboratory Cultivation Base Ministry of Science and Technology/Key Laboratory for Control Technology and Standard for Agro-product Safety and QualityMinistry of Agriculture and Rural Affairs/Key Laboratory for Agro-product Safety Risk Evaluation(Nanjing)Ministry of Agriculture and Rural Affairs/Collaborative Innovation Center for Modern Grain Circulation and Safety/Institute of Food Safety and NutritionJiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina School of Food and biological Engineering Jiangsu UniversityZhenjiangJiangsuChina

Pyruvate dehydrogenase kinase(PDK)is a mitochondrial enzyme in a variety of eukaryotes,including the plant pathogen Fusarium *** enzyme can reduce the oxidation of glucose to acetyl-coA by phosphorylation and selectively inhibits the activity of pyruvate dehydrogenase(PDH),which is a kind of pyruvate dehydrogenase complex(PDC).In this study,we investigated the *** pyruvate dehydrogenase kinase encoded by FgPDK2,which is a homologue of Neurospora crassa *** disruption of the FgPDK2 gene led to several phenotypic defects including effects on mycelial growth,conidiation,pigmentation,and *** mutants also showed decreased resistance to osmotic stress and cell membrane/wall-damaging *** FgPDK2 deletion mutant exhibited reduced *** of these defects were restored by genetic complementation of the mutant with the complete FgPDK2 ***,the results demonstrated that FgPDK2 is crucial for the growth of *** and can be exploited as a potential molecular target for novel fungicides to control Fusarium head blight caused by ***.

关键词： Fusarium graminearum Pyruvate dehydrogenase kinase Vegetative differentiation Reactive oxygen species(ROS)

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Modelling and simulation of two-bed PSA process for separating H2 from methane steam reforming

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Chinese Journal of Chemical Engineering 2019年第8期27卷 1870-1878页

作者： Huiru Li Zuwei Liao Jingyuan Sun Binbo Jiang Jingdai Wang Yongrong Yang Zhejiang Provincial Key laboratory of Advanced Chemical Engineering Manufacture Technology College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027 China State Key laboratory of Chemical Engineering College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027 China

Methane steam reforming is the main hydrogen production method in the industry. The product of methane steam reforming contains H2, CH4, CO and CO2 and is then purified by pressure swing adsorption(PSA) technology. In this study, a layered two-bed PSA process was designed theoretically to purify H2 from methane steam reforming off gas. The effects of adsorption pressure, adsorption time and purgeto-feed ratio(P/F ratio) on process performance were investigated to design a PSA process with more than99.95% purity and 80% recovery. Since the feed composition of the PSA process changes with the upstream process, the effect of the feed composition on the process performance was discussed as *** result showed that the increase of CH4 concentration, which was the weakest adsorbate, would have a negative impact on product purity.

关键词： Hydrogen Adsorption Purity Recovery Feed composition

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The shrimp IKK-NF-KB signaling pathway regulates antimicrobial peptide expression and may be subverted by white spot syndrome virus to facilitate viral gene expression

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Cellular & Molecular Immunology 2013年第5期10卷 423-436页

作者： Pei-Hui Wang Zhi-Hua Gu Ding-Hui Wan Bo-Du Liu Xian-De Huang Shao-Ping Weng Xiao-Qiang Yu Jian-Guo He MOE Key laboratory of Aquatic product Safety/State Key laboratory of Biocontrol School of Life Sciences Sun Yat-Sen University Guangzhou China Division of Cell Biology and Biophysics School of Biological Sciences University of Missouri-Kansas City Kansas City MO USA School of Marine Sciences Sun Yat-Sen University Guangzhou China

The IκB kinases IKKα and IKKβ and the IKK-related kinases TANK-binding kinase 1 （TBK1） and IKKε are the master regulators of the NF-κB signaling pathway. Although this pathway has been extensively studied in mammals, less attention has been paid in crustaceans, which have significant economic value. Here, we report the cloning and functional studies of two IKK homologs, LvlKKβ and LvlKKε, from Pacific white shrimp, Litopenaeus vannamei. LvlKKβ and LvlKKα mRNAs are widely expressed in different tissues and are responsive to white spot syndrome virus （WSSV） infection. When overexpressed in Drosophila S2 cells, LvlKKβ but not LvlKKε activates the promoters of NF-κB pathway-controlled antimicrobial peptide genes （AMPs）, such as the Penaeidins （PENs）. In HEK 293T cells, both LvlKKβ and LvlKKε activate an NF-κB reporter. The silencing of LvlKKβ or LvlKKε using double-stranded RNA （dsRNA）-mediated RNA interference （RNAi） decreases the expression of L. vannamei AMPs, including PENs, lysozyme and crustins. Intriguingly, LvlKKβ- or LvlKKε-silenced L. vannameiare resistant to WSSV infection. We hypothesized that successful infection with WSSV requires the activation of the IKK-NF-κB signaling pathway to modulate viral gene expression. We constructed luciferase reporters for 147 WSSV genes. By screening, we found that the WSSV051, WSSV059, WSSV069, WSSV083, WSSV090, WSSV107, WSSV244, WSSV303, WSSV371 and WSSV445 promoters can be activated by LvlKKβ or LvlKKε in Drosophila S2 cells. Taken together, our results reveal that LvlKKβ and LvlKKε may participate in the regulation of shrimp AMPs and that WSSV may subvert the L. vannamei IKK-NF-κB signaling pathway to facilitate viral gene expression.

关键词： antimicrobial peptide I KK-NF-KB innate immunity Litopenaeus vannamei WSSV

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