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Assessment of molecular markers and marker-assisted selection for drought tolerance in barley(Hordeum vulgare L.)

Journal of Integrative Agriculture 2024年第1期23卷 20-38页

作者： Akmaral Baidyussen Gulmira Khassanova Maral Utebayev Satyvaldy Jatayev Rystay Kushanova Sholpan Khalbayeva Aigul Amangeldiyeva Raushan Yerzhebayeva KulpashBulatova Carly Schramm Peter Anderson Colin L.D.Jenkins Kathleen LSoole Yuri Shavrukov Faculty of Agronomy S.Seifullin Kazakh AgroTechnical Research UniversityAstana 010000Kazakhstan A.I.Barayev research and Production Centre of Grain Farming Shortandy 021601Kazakhstan Kazakh research Institute of Agriculture and Plant Growing AlmalybakAlmaty District 040909Kazakhstan College of Science and Engineering Biological SciencesFlinders UniversityAdelaideSA 5042Australia

This review updates the present status of the field of molecular markers and marker-assisted selection(MAS),using the example of drought tolerance in *** accuracy of selected quantitative trait loci(QTLs),candidate genes and suggested markers was assessed in the barley genome *** common strategies are described for molecular marker development,candidate gene identification and verification,and their possible applications in MAS to improve the grain yield and yield components in barley under drought *** strategies are based on the following five principles:(1)Molecular markers are designated as genomic‘tags’,and their‘prediction’is strongly dependent on their distance from a candidate gene on genetic or physical maps;(2)plants react differently under favourable and stressful conditions or depending on their stage of development;(3)each candidate gene must be verified by confirming its expression in the relevant conditions,e.g.,drought;(4)the molecular marker identified must be validated for MAS for tolerance to drought stress and improved grain yield;and(5)the small number of molecular markers realized for MAS in breeding,from among the many studies targeting candidate genes,can be explained by the complex nature of drought stress,and multiple stress-responsive genes in each barley genotype that are expressed differentially depending on many other factors.

关键词： barley candidate genes drought tolerance gene verification via expression grain yield marker-assisted selection(MAS) molecular markers quantitative trait loci(QTLs) strategy for MAS

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Characterization of blueberry exosome-like nanoparticles and miRNAs with potential cross-kingdom human gene targets

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Food Science and Human Wellness 2024年第2期13卷 869-878页

作者： Yangfan Leng Liubin Yang Siyi Pan Leilei Zhan Fang Yuan College of Food Science and Technology Huazhong Agricultural UniversityWuhan 430070China Hubei Key Laboratory of Fruit&Vegetable Processing&Quality control Huazhong Agricultural UniversityWuhan 430070China Wuhan GeneCreate biological Engineering Co. Ltd.Wuhan 430070China

Edible plant derived exosome-like nanoparticles(ELNs)have been shown to have multiple nutraceutical ***,the diversity of plant materials makes the plant derived ELN study *** efforts are still needed to explore the feasible isolation methods of edible plant derived ELNs and the possible roles of food-derived ELNs in improving human *** this study,a size exclusion chromatography based method was compared with the traditional ultracentrifugation method to isolate blueberry derived ELNs(B-ELNs),and the miRNA profile of B-ELNs was analyzed by high-throughput sequencing.A total of 36 miRNAs were found to be enriched in B-ELNs compared with berry tissue,and their potential cross-kingdom human gene targets were further *** showed that size exclusion chromatography was effective for B-ELN *** most abundant miRNAs in B-ELNs mainly belonged to the miR166 family and miR396 *** gene prediction indicated that B-ELNs could potentially regulate pathways related to the human digestive system,immune system and infectious diseases.

关键词： Edible plant derived exosomes-like nanoparticles Size exclusion chromatography miRNA Target gene prediction Blueberry

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KIF5B-mediated internalization of FMDV promotes virus infection

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Virologica Sinica 2024年第3期39卷 378-389页

作者： Wei Zhang Fan Yang Yang Yang Weijun Cao Wenhua Shao Jiali Wang Mengyao Huang Zhitong Chen Xiaoyi Zhao Weiwei Li Zixiang Zhu Haixue Zheng State Key Laboratory for Animal Disease control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000China Gansu Province research Center for Basic Disciplines of Pathogen Biology Lanzhou 730046China

Foot-and-mouth disease(FMD)is a highly contagious and economically important disease,which is caused by the FMD virus(FMDV).Although the cell receptor for FMDV has been identified,the specific mechanism of FMDV internalization after infection remains *** this study,we found that kinesin family member 5B(KIF5B)plays a vital role during FMDV ***,we confirmed the interaction between KIF5B and FMDV structural protein VP1 by co-immunoprecipitation(Co-IP)and co-localization in FMDV-infected *** particular,the stalk[amino acids(aa)413–678]domain of KIF5B was indispensable for KIF5B-VP1 ***,overexpression of KIF5B dramatically enhanced FMDV replication;consistently,knockdown or knockout of KIF5B suppressed FMDV ***,we also demonstrated that KIF5B promotes the internalization of FMDV via regulating clathrin ***5B also promotes the transmission of viral particles to early and late endosomes during the early stages of *** conclusion,our results demonstrate that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating and intracellular *** study may provide a new therapeutic target for developing FMDV antiviral drugs.

关键词： FMDV VP1 protein KIF5B Endosome Clathrin

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Emergence of Erythromycin-Resistant and Pertactin-and Filamentous Hemagglutinin-Deficient Bordetella pertussis Strains—Beijing,China,2022-2023

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China CDC weekly 2024年第20期6卷 437-441页

作者： Guilan Zhou Ying Li Hairui Wang Yuanyuan Wang Yuan Gao Juan Xu Fengshuang Wang Tao Peng Maojun Zhang Zhujun Shao National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Shunyi District Center for Disease control and Prevention BeijingChina

What is already known about this topic?Pertussis has reemerged as a significant public health threat,primarily due to variations in Bordetella pertussis strains,antimicrobial resistance,and vaccine *** is added by this report?All isolated strains were identified as ptxA1/ptxC2/ptxP3/prn150/fim2-1/fim3-1/fhaB1/tcfA2 type and exhibited resistance to erythromycin.

关键词： vaccine pertussis primarily

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Neglected Time Intervals Before “1-3-7” Approach About Imported Malaria Cases-China, 2014–2021

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China CDC weekly 2024年第17期6卷 363-367页

作者： Zhiquan He Yuanjing Kou Dan Wang Ying Liu Xiaofeng Lyu Hui Yan Wen Lin Xuan Zhang Duoquan Wang Shizhu Li Hongwei Zhang Henan Provincial Center for Disease control and Prevention Zhengzhou CityHenan ProvinceChina Anhui Provincial Center for Disease control and Prevention Hefei CityAnhui ProvinceChina Guangxi Zhuang autonomous Region Center for Disease control and Prevention Nanning CityGuangxi Zhuang Autonomous RegionChina Hubei Provincial Center for Disease control and Prevention Wuhan CityHubei ProvinceChina Zhejiang Provincial Center for Disease control and Prevention Hangzhou CityZhejiang ProvinceChina National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)National Health Commission Key Laboratory of Parasite and Vector BiologyShanghaiChina School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghaiChina

What is already known about this topic?China’s“1-3-7”approach outlines specific targets to guide and monitor the processes of case reporting,investigation,and ***,few studies have examined the time intervals preceding the initial step,and the timeline from the arrival of imported malaria cases in China to their diagnosis has been largely *** is added by this report?The study demonstrated that the median duration from arrival in China to the onset of symptoms for *** was 78 days,with 71.59%of imported cases manifesting symptoms after more than one *** ***,the median interval was 42 days,with 55.91%exceeding one ***,the median time from symptom onset to malaria treatment in China between 2014 and 2021 was 2 days,with an interquartile range(IQR)of 1-4 *** are the implications for public health practice?This study represents the initial effort to delineate the chronology of imported malaria cases,from their arrival in China to their subsequent *** results underscore the importance of providing malaria health education to populations arriving from ***,enhancing physician training is crucial for improving the diagnosis of malaria.

关键词： diagnosis Malaria exceeding

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Study of the multi-physics field-coupled model of the two-stage electrostatic precipitator

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Building Simulation 2024年第3期17卷 387-398页

作者： Wenjia Hao Yu Guo Yukun Wang Tao Yu Hao Gao Zhengwei Long Tianjin Key Laboratory of Indoor Air Environmental Quality control School of Environmental Science and EngineeringTianjin UniversityWeijin Road 92Tianjin300072China Wuhan Second Ship Design and research Institute WuhanChin

The two-stage electrostatic precipitator is widely used to purify oil mist ***,there is limited research on the influences of relative humidity,particle deposition characteristics,and the generation of gaseous ***,this paper established a numerical model of the electrostatic oil mist purifier and applied it to a two-stage electrostatic *** the model was used to investigate the corona discharge characteristics under different relative humidity conditions in the charged zone,the particle deposition characteristics,the purification efficiency,the ozone concentration distribution,and the oil vapor concentration distribution in the collection *** results indicate that,with a constant temperature,the corona current decreases as relative humidity increase,and there is a quadratic relationship between relative humidity and *** variation in relative humidity has little impact on the purification *** maximum ozone concentration occurs near the electrode line,and its concentration is influenced by the discharge current and inlet airflow *** oil vapor concentration reaches its maximum value at the side plates,with a value of 19 ppb,while it reaches the minimum value at the collecting zone electrode plate,with a value of 2 *** temperature is the main factor affecting the volatilization of the oil film,with higher temperatures resulting in higher oil vapor.

关键词： two-stage ESPs particle deposition corona discharge oil vapor

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Underwater four-quadrant dual-beam circumferential scanning laser fuze using nonlinear adaptive backscatter filter based on pauseable SAF-LMS algorithm

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Defence Technology（防务技术） 2024年第7期37卷 1-13页

作者： Guangbo Xu Bingting Zha Hailu Yuan Zhen Zheng He Zhang Ministerial Key Laboratory of ZNDY Nanjing University of Science and TechnologyNanjing 210094China Science and Technology on Electromechanical Dynamic control Laboratory Beijing 100081China Shanghai Radio Equipment research Institute Shanghai 201109China

The phenomenon of a target echo peak overlapping with the backscattered echo peak significantly undermines the detection range and precision of underwater laser *** overcome this issue,we propose a four-quadrant dual-beam circumferential scanning laser fuze to distinguish various interference signals and provide more real-time data for the backscatter filtering *** enhances the algorithm loading capability of the *** order to address the problem of insufficient filtering capacity in existing linear backscatter filtering algorithms,we develop a nonlinear backscattering adaptive filter based on the spline adaptive filter least mean square(SAF-LMS)*** also designed an algorithm pause module to retain the original trend of the target echo peak,improving the time discrimination accuracy and anti-interference capability of the ***,experiments are conducted with varying signal-to-noise ratios of the original underwater target echo *** experimental results show that the average signal-to-noise ratio before and after filtering can be improved by more than31 d B,with an increase of up to 76%in extreme detection distance.

关键词： Laser fuze Underwater laser detection Backscatter adaptive filter Spline least mean square algorithm Nonlinear filtering algorithm

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Regression Method for Rail Fastener Tightness Based on Center-Line Projection Distance Feature and Neural Network

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Chinese Journal of Mechanical Engineering 2024年第2期37卷 356-371页

作者： Yuanhang Wang Duxin Liu Sheng Guo Yifan Wu Jing Liu Wei Li Hongjie Wang Robotics research Center School of MechanicalElectronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China China Construction Industrial&Energy Engineering Group Co. Ltd.Nanjing 210023China

In the railway system,fasteners have the functions of damping,maintaining the track distance,and adjusting the track ***,routine maintenance and inspection of fasteners are important to ensure the safe operation of track ***,assessment methods for fastener tightness include manual observation,acoustic wave detection,and image *** are limitations such as low accuracy and efficiency,easy interference and misjudgment,and a lack of accurate,stable,and fast detection *** at the small deformation characteristics and large elastic change of fasteners from full loosening to full tightening,this study proposes high-precision surface-structured light technology for fastener detection and fastener deformation feature extraction based on the center-line projection distance and a fastener tightness regression method based on neural ***,the method uses a 3D camera to obtain a fastener point cloud and then segments the elastic rod area based on the iterative closest point algorithm *** component analysis is used to calculate the normal vector of the segmented elastic rod surface and extract the point on the centerline of the elastic *** point is projected onto the upper surface of the bolt to calculate the projection ***,the mapping relationship between the projection distance sequence and fastener tightness is established,and the influence of each parameter on the fastener tightness prediction is ***,by setting up a fastener detection scene in the track experimental base,collecting data,and completing the algorithm verification,the results showed that the deviation between the fastener tightness regression value obtained after the algorithm processing and the actual measured value RMSE was 0.2196 mm,which significantly improved the effect compared with other tightness detection methods,and realized an effective fastener tightness regression.

关键词： Railway system Fasteners Tightness inspection Neural network regression 3D point cloud processing

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Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

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World Journal of Stem Cells 2024年第4期16卷 410-433页

作者： Shafiqa Naeem Rajput Bushra Kiran Naeem Anwar Ali Asmat Salim Irfan Khan Dr.Panjwani Center for Molecular Medicine and Drug research International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270SindhPakistan Surgical unit 4 Dr.Ruth KM Pfau Civil HospitalKarachi 74400Pakistan Department of Physiology University of KarachiKarachi 75270Pakistan Center for Regenerative Medicine and Stem Cells research and Department of Ophthalmology and Visual SciencesThe Aga Khan UniversityKarachi 74800SindhPakistan

BACKGROUND Stem cells are undifferentiated cells that possess the potential for self-renewal with the capacity to differentiate into multiple *** humans,their limited numbers pose a challenge in fulfilling the necessary demands for the regeneration and repair of damaged tissues or *** suggested that mesenchymal stem cells(MSCs),necessary for repair and regeneration via transplantation,require doses ranging from 10 to 400 million ***,the limited expansion of MSCs restricts their therapeutic *** To optimize a novel protocol to achieve qualitative and quantitative expansion of MSCs to reach the targeted number of cells for cellular transplantation and minimize the limitations in stem cell therapy *** Human umbilical cord(hUC)tissue derived MSCs were obtained and *** cultured cells were subjected to the following evaluation pro-cedures:Immunophenotyping,immunocytochemical staining,trilineage differentiation,population doubling time and number,gene expression markers for proliferation,cell cycle progression,senescence-associatedβ-galactosidase assay,human telomerase reverse transcriptase(hTERT)expression,mycoplasma,cytomegalovirus and endotoxin *** Analysis of pluripotent gene markers Oct4,Sox2,and Nanog in recultured hUC-MSC revealed no significant *** immunophenotypic markers CD90,CD73,CD105,CD44,vimentin,CD29,Stro-1,and Lin28 were positively expressed by these recultured expanded MSCs,and were found negative for CD34,CD11b,CD19,CD45,and *** recultured hUC-MSC population continued to expand through passage *** gene expression of Pax6,BMP2,and TGFb1 showed no significant variation between recultured hUC-MSC ***,a significant increase(P<0.001)in the mitotic phase of the cell cycle was observed in recultured *** senescence markers(hTERT expression andβ-galactosidase activity)did not show any negative effect on recultured hUC-

关键词： Human umbilical cord Mesenchymal stem cells Expansion Cell proliferation In vitro expansion Senescence

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research on concrete structure defect repair based on three-dimensional printing

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Frontiers of Structural and Civil Engineering 2024年第5期18卷 731-742页

作者： Yang GU Wei LI Xupeng YAO Guangjun LIU School of Mechanical Engineering Tongji UniversityShanghai 201804China College of Civil Engineering Tongji UniversityShanghai 200092China Shanghai Engineering research Center for Safety Intelligent control of Building Machinery Shanghai 200032China

Quality assurance and maintenance play a crucial role in engineering construction,as they have a significant impact on project *** common issue in concrete structures is the presence of *** enhance the automation level of concrete defect repairs,this study proposes a computer vision-based robotic system,which is based on three-dimensional(3D)printing technology to repair *** system integrates multiple sensors such as light detection and ranging(LiDAR)and *** is utilized to model concrete pipelines and obtain geometric parameters regarding their ***,a convolutional neural network(CNN)is employed with a depth camera to locate defects in concrete ***,a method for coordinate transformation is presented to convert the obtained coordinates into executable ones for a robotic ***,the feasibility of this concrete defect repair method is validated through simulation and experiments.

关键词： concrete defect detection 3D printing deep learning point cloud data

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