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Effect of temperature on antioxidant defense and innate immunity in Brandt’s voles

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Zoological Research 2019年第4期40卷 305-316页

作者： De-Li Xu Meng-Meng Xu De-Hua Wang College of Life sciences Qufu Normal UniversityQufu Shandong 273165China State Key Laboratory of Integrated management of pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China

Ambient temperature is an importa nt factor in flue ncing many physiological processes, in eluding antioxidant defense and immunity. In the present study, we tested the hypothesis that antioxidant defense and immunity are suppressed by high and low temperature treatme nt in Bran dt's voles (Lasiopodomys brandtii). Thirty male voles were randomly assigned into different temperature groups (4, 23, and 32℃, n=10 for each group), with the treatment course lasting for 27 d. Results showed that low temperature in creased gross en ergy in take (GEI) and liver, heart, and kidney mass, but decreased body fat mass and dry carcass mass. With the decline in temperature, hydrogen peroxide (H2O2) concentration, which is indicative of reactive oxyge n species (ROS) levels, in creased in the liver, decreased in the heart, and was unchanged in the kidney, testis, and small intestine. Lipid peroxidation indicated by malonaldehyde (MDA) content in the liver, heart, kidney, testis, and small intestine did not differ among groups, implying that high and low temperature did not cause oxidative damage. Similarly, superoxide dismutase (SOD) and catalase (CAT) activities and total antioxidant capacity (TAOC) in the five tissues did not respond to low or high temperature, except for elevation of CAT activity in the testis upon cold exposure. Bacteria killing capacity, which is indicative of innate immunity, was nearly suppressed in the 4℃ group in contrast to the 23℃ group, whereas spleen mass and white blood cells were un affected by temperature treatment. The levels of testosterone, but not corticostero ne, were in flue need by temperature treatment, though neither were correlated with innate immunity, H2O2 and MDA levels, or SOD, CAT, and TAOC activity in any detected tissues. Overall, these results showed that temperature had different in flue nces on oxidative stress, an tioxida nt en zymes, and immunity, which depended on the tissues and parameters tested. Up-regulation or maintenance

关键词： Antioxidant defense Brandt's voles(Lasiopodomys brandtii) Immunity Temperature

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Use of ryegrass strips to enhance biological control of aphids by ladybirds in wheat fields

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Insect Science 2012年第4期19卷 529-534页

作者： Zhao-Ke Dong Feng-Juan Gao Run-Zhi Zhang Key Laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of Sciences Graduate University ofChinese Academy of sciences State Key Laboratory of Integrated management of pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina

Non-crop habitats may play a vital role in conservation biological control. This study tested the effect of ryegrass （Lolium multiflorum L.） strips on aphid and la- dybird populations in adjacent winter wheat fields. The field experiment was conducted in three ryegrass-margin wheat plots and three control plots in 2010 in North China. In spring, the same aphid species, Sitobion miscanthi （Takahashi）, was found in both the ryegrass strips and wheat plots. The population density of ladybirds in the ryegrass strips （3.5 4- 0.9/m2） was significantly higher than in the wheat plots （1.5 4- 0.5/m2）. We cut the ryegrass, forcing the ladybirds to migrate to the wheat fields. Three and eight days after cutting the ryegrass, the aphid numbers in the ryegrass-margin wheat plots decreased significantly： they were 19.9% and 53.6%, respectively, lower than in control plots. In the early period of ladybird population development, the percentage of larvae was greater in the ryegrass-margin wheat plots than in controls, and the peak number of pupae in the ryegrass-margin wheat plots occurred 5 days earlier than in the control plots. The results suggest that ryegrass strips may promote the development of ladybird populations. Cutting ryegrass can manipulate ladybirds to enhance biological aphid control in wheat fields. The efficiency of this management approach is discussed.

关键词： coccinellids habitat management natural enemies non-crop habitat

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Current Zoology 2019年第5期65卷 527-534页

作者： Ke DENG Wei LIU De-Hua WANG State Key Laboratory of Integrated management of pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina Chengdu Institute of Biology Chinese Academy of SciencesChengduSichuanChina University of Chinese Academy of sciences BeijingChina

Kin selection theory predicts that individuals should generally behave less aggressively or more amicably towards relatives than ***,how individuals treat conspecifics depends on genetic relatedness but also on the ecological context,which influences the benefits and costs of their *** this study,we used microsatellite DNA markers and behavioral tests to examine the influence of kinship and proximity on the social behavior of Mongolian gerbils Meriones unguiculatus living in different social groups,and whether these effects varied with sex and *** recorded the duration of 4 behavioral categories(investigative,neutral,amicable,and agonistic)during a 10-min pairwise *** found that genetic relatedness had significant effects on the duration of investigative,neutral,and amicable behavior,but not on agonistic *** also found significant interaction effects of relatedness and distance between burrow systems(i.e.,spatial distance)on investigative,neutral,and amicable behavior,which suggests that the effects of kinship on social behavior were restricted by spatial *** interaction effect between sex and relatedness on amicable behavior showed that male gerbils became more intimate with individuals of the same sex that had higher pairwise relatedness than ***,both male and female gerbils enhanced their aggression during the food-hoarding season,but the intensity of these changes was significantly higher in ***,our results suggest that the effects of kinship and spatial proximity on social behavior exhibit sexual or seasonal patterns,thereby implying ecological context-dependent responses to out-group individuals in Mongolian gerbils.

关键词： familiarity kinship Meriones unguiculatus seasonality social behavior

Major ascaroside pheromone component asc-C5 influences reproductive plasticity among isolates of the invasive species pinewood nematode

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Integrative Zoology 2021年第6期16卷 893-907页

作者： Meiping ZHAO Jacob D.WICKHAM Lilin ZHAO Jianghua SUN State Key Laboratory of Integrated management of pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijingChina

Pheromones are communication chemicals and regulatory signals used by animals and represent unique tools for organisms to mediate behaviors and make“decisions”to maximize their *** plasticity refers to the innate capacity of a species to tolerate a greater breadth of environmental conditions across which it adapts to improve its survival,reproduction,and *** pinewood nematode,Bursaphelenchus xylophilus,an invasive nematode species,was accidentally introduced from North America into Japan,China,and Europe;however,few studies have investigated its pheromones and phenotypic plasticity as a natural ***,we demonstrated a novel phenomenon,in which nematodes under the condition of pheromone presence triggered increased reproduction in invasive strains(JP1,JP2,CN1,CN2,EU1,and EU2),while it simultaneously decreased reproduction in native strains(US1 and US2).The bidirectional effect on fecundity,mediated by presence/absence of pheromones,is henceforth termed pheromone-regulative reproductive plasticity(PRRP).We further found that synthetic ascaroside asc-C5(ascr#9),the major pheromone component,plays a leading role in PRRP and identified 2 candidate receptor genes,Bxydaf-38 and Bxysrd-10,involved in perceiving *** results suggest that plasticity of reproductive responses to pheromones in pinewood nematode may increase its fitness in novel environments following *** opens up a new perspective for invasion biology and presents a novel strategy of invasion,suggesting that pheromones,in addition to their traditional roles in chemical signaling,can influence the reproductive phenotype among native and invasive *** addition,this novel mechanism could broadly explain,through comparative studies of native and invasive populations of animals,a potential underlying factor behind of the success of other biological invasions.

关键词： Bursaphelenchus xylophilus fecundity invasion phenotypic plasticity pheromone

Effects of methamidophos and deltamethrin on in vitro protein phosphorylation in Monochamus alternatus

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Insect Science 2008年第6期15卷 529-535页

作者： Jie Liu Xi-Wu Gao Yi-Jun Wu Wei Li Qi-Lian Qin Jiang-Hua Sun State Key Laboratory of Integrated management of pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing College of Agronomy and Biotechnology China Agricultural University Beijing China

Monochamus alternatus Hope （Coleoptera： Cerambycidae） is not only a serious pest insect to pine trees but also the main vector of pine wood nemadote Bursaphelenchus xylophilus, which causes pine wilt disease. To explore the insecticidal mechanism of insecticides to M. alternatus, we chose methamidophos and deltamethrin as the representatives of two groups of insecticides （organophosphates and pyrethroids）, which are widely used for pest control in China and investigated their effects on phosphorylation of proteins from the insect. Phosphorylation of proteins from the insect fat body and head was determined by in vitro 32P-labelling. In the fat body, deltamethrin obviously reduced basal phosphorylation levels of proteins at 111, 95, 77, and 44 kDa, but enhanced the basal phosphorylation level of a protein at 138 kDa. However, in the presence of calmodulin but not cyclic adenosine monophosphate （cAMP）, deltamethrin increased phosphorylation of the protein at 111 kDa. In the head, deltamethrin inhibited basal phosphorylation levels of proteins at 113, 98, and 51 kDa, but potentiated phosphorylation of a protein at 167 kDa activated by cAMP. Methamidophos inhibited phosphorylation of a protein at 44 kDa in the fat body. Although methamidophos did not impact basal phosphorylation levels of any proteins in the head, it inhibited calcium/calmodulin （Ca^2＋CaM）-stimulated phosphorylation of a protein at 51 kDa. Together, our data indicate that methamidophos and deltamethrin altered phosphorylation levels of various proteins in the head and fat body of the pine insect and these two kinds of insecticides acted on the proteins that can be phosphorylated in the tissues respectively, which is possibly related to their toxicity.

关键词： methamidophos deltamethrin Monochamus alternatus protein phosphorylation

Peficitinib suppresses diffuse-type tenosynovial giant cell tumor by targeting TYK2 and JAK/STAT signaling

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Science China Life sciences 2025年

作者： Shan Lu Chenxi Cao Wenjia Zhang Jiayi Li Jingli Yang Zisheng Huang Zhijun Wu Baitao Liu Hongjie Huang Haijun Wang Yongjian Wang Dingge Liu Zhihua Zhang Kaiping Liu Gang Yang Xi Gong Hui Dai Yingjia Li Erdan Dong Xin Zhang Yan Zhang Haihe Laboratory of Cell Ecosystem Institute of Cardiovascular Diseases The First Affiliated Hospital of Dalian Medical University Institute of Cardiovascular sciences School of Basic Medical Sciences Peking University Health Science Center Department of Cardiology and Institute of Vascular Medicine Peking University Third HospitalState Key Laboratory of Vascular Homeostasis and Remodeling Peking University Department of Sports Medicine Peking University Third Hospital Institute of Sports Medicine of Peking University Beijing Key Laboratory of Sports Injuries Engineering Research Center of Sports Trauma Treatment Technology and Devices Ministry of Education State Key Laboratory of Membrane Biology Institute of Molecular Medicine College of Future Technology Peking University Peking University Health Science Centre Peking University Department of Immunology School of Basic Medical Sciences NHC Key Laboratory of Medical Immunology Peking University Research Center for Cardiopulmonary Rehabilitation University of Health and Rehabilitation Sciences Qingdao Hospital (Qingdao Municipal Hospital) School of Health and Life Sciences University of Health and Rehabilitation Sciences Research Unit of Medical Science Research management/Basic and Clinical Research of Metabolic Cardiovascular Diseases Chinese Academy of Medical Sciences

Diffuse-type tenosynovial giant cell tumor(dTGCT) is a destructive but rare benign proliferative synovial neoplasm. Although surgery is currently the main treatment modality for dTGCT, the recurrence risk is up to 50%. Therefore, there is a great need for effective drugs against dTGCT with minor side effects. The Janus kinase(JAK)/signal transducer and activator of transcription(STAT) signaling plays a central role in rheumatoid arthritis(RA), a disease with similar characteristics as dTGCT, but its function in dTGCT remains *** fibroblast-like synoviocytes(FLS) and macrophages were isolated from 10 synovial tissue samples from dTGCT patients for the screening and validation of the five clinically approved JAK inhibitors to treat RA against dTGCT. Cell viability, cell death, inflammation and the activity of the JAK family members of cultured dTGCT FLS(both 2-D and 3-D) and macrophages were investigated for the efficacy of the JAK inhibitors. Here, we found that similar to RA, JAK/STAT signaling was markedly activated in the dTGCT synovium. Of the 5 JAK inhibitors, peficitinib was shown to have the most potency in addressing some of the pathological responses of dTGCT FLS and *** potency of peficitinib was much higher than pexidartinib, which is the only FDA-approved drug for dTGCT. Mechanistically, peficitinib inhibited tyrosine kinase 2(TYK2), a JAK family member necessary for the pathological progression of dTGCT FLS and macrophages. In summary, we not only revealed JAK/STAT(especially TYK2) signaling as the major mechanism underlying dTGCT, but also identified peficitinib as a promising drug against dTGCT.

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MicroRNA-dependent development revealed by RNA interference-mediated gene silencing of LmDicerl in the migratory locust

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Insect Science 2013年第1期20卷 53-60页

作者： Yan-Li Wang Mei-Ling Yang Feng Jiang Jian-Zhen Zhang Le Kang Research Institute of Applied Biology Shanxi University Taiyuan State Key Laboratory of Integrated management of pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

MicroRNAs （miRNAs） are small noncoding RNAs, which participate in many biological processes. The small RNA transcriptome in the migratory locust has been characterized and 50 conserved miRNA families and 185 potential locust-specific miRNA family candidates have been identified using high-throughput sequencing. However, it is unclear whether miRNAs influence a wide variety of locusts＇ biological processes, such as growth or development. In insects, Dicerl ribonuclease transforms miRNA precursors into mature miRNAs. Thus, using systemic RNA interference （RNAi） to silence the expression of Dicer1 in the migratory locust, Locusta migratoria, we reduced miRNA contents in the locust and disrupted two types of molt （nymph-nymph, and nymph-adult）. The RNAi of LmDicerl also resulted in a high mortality in L. migratora. Our study revealed that LmDicerl was essential for miRNA regulation and development ofL. migratoria. These results further support our notion that LmDicerl could serve as an excellent target for developing novel strategies for controlling this important insect pest.

关键词： development dicerl Locusta migratoria microRNA

Evaluation of Beauveria bassiana （Hyphomycetes） isolates as potential agents for control of Dendroctonus valens

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Insect Science 2011年第2期18卷 209-216页

作者： Long-Wa Zhang Yu-Jun Liu Jian Yao Bin Wang Bo Huang Zeng-Zhi Li Mei-Zhen Fan Jiang-Hua Sun Anhui Provincial Key Laboratory of Microbial pest Control Anhui Agricultural University Heifei Anhui Entry-Exit Inspection and Quaran- tine Bureau Hefei State Key Laboratory of Integrated management Agricultural pest & Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

The red turpentine beetle （RTB）, Dendroctonus valens LeConte, as a destructive invasive pest, has become one of the most economically important forest pest in China. Effective control measures are desperately needed. Entomopathogenic fungi, such as Beauveria bassiana, have shown great potential for the management of some bark beetle species. In this study, 12 isolates of B. bassiana from bark beetle were examined for biological characteristics and virulence, to assess their potential as biocontrol agents for RTB. There were significant differences （at P = 0.05） in colony growth rate, conidial yield, conidial germination, tolerance to UV light and extracellular proteases activity among the tested B. bassiana isolates. Isolates, including Bbl801, Bb1906, Bb789 and Bb773, exhibited the best characteristics, because they have faster hyphal growth rate, higher spore production and faster spore germination, higher UV tolerance and protease （Prl） production. The results of a pathogenicity test ofB. bassiana on RTB larvae showed that most isolates of B. bassiana have demonstrated high efficacy and the highest virulent isolate was Bb 1801, which killed 100% of the treated insects and had a median lethal time （LT50） of 4.60 days at a concentration of 1 ×107 conidia/mL. Therefore, isolate Bb1801 has a great potential for sustainable control of RTB in the forest. The correlation between biological characteristics and virulence of the fungal isolates is discussed and the possibility of combination of entomopathogenic fungi with semiochemicals, as one of the promising strategy for RTB control, is considered.

关键词： Beauveria bassiana biological characteristics microbial control Dendroctonus valens virulence

Receptor variability-driven evolution of snake toxins

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Zoological Research 2018年第6期39卷 431-436页

作者： Xian-Hong Ji Shang-Fei Zhang Bin Gao Shun-Yi Zhu Group of Peptide Biology and Evolution State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of Sciences Beijing 100101 China University of Chinese Academy of sciences Beijing 100049 China

Three-finger toxins （TFTs） are well-recognized non- enzymatic venom proteins found in snakes. However, although TFTs exhibit accelerated evolution, the drivers of this evolution remain poorly understood. The structural complexes between long-chain α-neurotoxins, a subfamily of TFTs, and their nicotinic acetylcholine receptor targets have been determined in previous research, providing an opportunity to address such questions. In the current study, we observed several previously identified positively selected sites （PSSs） and the highly variable C-terminal loop of these toxins at the toxin/receptor interface. Of interest, analysis of the molecular adaptation of the toxin-recognition regions in the corresponding receptors provided no statistical evidence for positive selection. However, these regions accumulated abundant amino acid variations in the receptors from the prey of snakes, suggesting that accelerated substitution of TFTs could be a consequence of adaptation to these variations. To the best of our knowledge, this atypical evolution, initially discovered in scorpions, is reported in snake toxins for the first time and may be applicable for the evolution of toxins from other venomous animals.

关键词： Three-finger toxins Nicotinic acetylcholine receptor Driver

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