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Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

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Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu Rui Wang Hanyue Zhang Xiou Wang Sijia Zhou Bo Ma Yan Luan Xin Wang Xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

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Multiple Tin Compounds Modified Carbon Fibers to Construct Heterogeneous Interfaces for Corrosion Prevention and Electromagnetic Wave Absorption

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Nano-Micro Letters 2025年第1期17卷 507-527页

作者： Zhiqiang Guo Di Lan Zirui Jia Zhenguo Gao Xuetao Shi Mukun He Hua Guo Guanglei Wu Pengfei Yin College of Science Sichuan Agricultural UniversityYa’an 625014People’s Republic of China School of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002People’s Republic of China institute of Materials for Energy and Environment State Key Laboratory of Bio‑Fibers and Eco‑TextilesCollege of Materials Science and EngineeringQingdao UniversityQingdao 266071People’s Republic of China shaanxi Key Laboratory of Macromolecular Science and Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’anShaanxi 710072People’s Republic of China

Currently,the demand for electromagnetic wave(EMW)absorbing materials with specific functions and capable of withstanding harsh environments is becoming increasingly ***-component interface engineering is considered an effective means to achieve high-efficiency EMW ***,interface modulation engineering has not been fully discussed and has great potential in the field of EMW *** this study,multi-component tin compound fiber composites based on carbon fiber(CF)substrate were prepared by electrospinning,hydrothermal synthesis,and high-temperature thermal *** utilizing the different properties of different substances,rich heterogeneous interfaces are *** effectively promotes charge transfer and enhances interfacial polarization and conduction *** prepared SnS/SnS_(2)/SnO_(2)/CF composites with abundant heterogeneous interfaces have and exhibit excellent EMW absorption properties at a loading of 50 wt%in epoxy *** minimum reflection loss(RL)is−46.74 dB and the maximum effective absorption bandwidth is 5.28 ***,SnS/SnS_(2)/SnO_(2)/CF epoxy composite coatings exhibited long-term corrosion resistance on Q235 steel ***,this study provides an effective strategy for the design of high-efficiency EMW absorbing materials in complex and harsh environments.

关键词： Electrostatic spinning Component regulation Heterogeneous interfaces Electromagnetic wave absorption Corrosion protection

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Potential global distribution of the guava root-knot nematode Meloidogyne enterolobii under different climate change scenarios using MaxEnt ecological niche modeling

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Journal of Integrative agriculture 2023年第7期22卷 2138-2150页

作者： PAN Song PENG De-liang LI Ying-mei CHEN Zhi-jie ZHAI Ying-yan LIU Chen HONG Bo shaanxi Key Laboratory of Plant Nematology Bio-Agriculture Institute of ShaanxiShaanxi Academy of SciencesXi’an 710043P.R.China State Key Laboratory for biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

In recent years,Meloidogyne enterolobii has emerged as a major parasitic nematode infesting many plants in tropical or subtropical ***,the regions of potential distribution and the main contributing environmental variables for this nematode are *** the current climate scenario,we predicted the potential geographic distributions of *** worldwide and in China using a Maximum Entropy(MaxEnt)model with the occurrence data of this ***,the potential distributions of *** were projected under three future climate scenarios(BCC-CSM2-MR,CanESM5 and CNRM-CM6-1)for the periods 2050s and *** in the potential distribution were also predicted under different climate *** results showed that highly suitable regions for *** were concentrated in Africa,South America,Asia,and North America between latitudes 30°S to 30°***16(precipitation of the wettest quarter),bio10(mean temperature of the warmest quarter),and bio11(mean temperature of the coldest quarter)were the variables contributing most in predicting potential distributions of *** addition,the potential suitable areas for *** will shift toward higher latitudes under future climate *** study provides a theoretical basis for controlling and managing this nematode.

关键词： Meloidogyne enterolobii species distribution model MaxEnt climate change future climate scenarios centroid change

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Prediction of Photosynthetic Carbon Assimilation Rate of Individual Rice Leaves under Changes in Light Environment Using BLSTM-Augmented LSTM

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Computer Modeling in Engineering & Sciences 2022年第12期133卷 557-577页

作者： Masayuki Honda Kenichi Tatsumi Masaki Nakagawa Graduate School of bio-Applications and Systems Engineering Tokyo University of Agriculture and TechnologyKoganei City184-8588Japan institute of agriculture Tokyo University of Agriculture and TechnologyFuchu City183-0054Japan institute of Engineering Tokyo University of Agriculture and TechnologyKoganei City184-8588Japan

A model to predict photosynthetic carbon assimilation rate(A)with high accuracy is important for forecasting crop yield and *** short-term memory(LSTM),a neural network suitable for time-series data,enables prediction with high accuracy but requires mesophyll *** addition,for practical use,it is desirable to have a technique that can predict A from easily available *** this study,we propose a BLSTM augmented LSTM(BALSTM)model,which utilizes bi-directional LSTM(BLSTM)to indirectly reproduce the mesophyll variables required for *** most significant feature of the proposed model is that its hybrid architecture uses only three relatively easy-to-collect external environmental variables—photosynthetic photon flux density(Q_(in)),ambient CO_(2) concentration(C_(a)),and temperature(T_(air))—to generate mesophyll CO_(2) concentration(C_(i))and stomatal conductance to water vapor(g_(sw))as intermediate ***,A is predicted by applying the obtained intermediate outputs to the learning ***,in this study,1)BALSTM(Q_(in),C_(a),T_(air))had a significantly higher A prediction accuracy than LSTM(Q_(in),C_(a),T_(air))in case of using only Q_(in),C_(a),and T_(air);2)BALSTMC_(i),g_(sw),which had C_(i) and g_(sw) as intermediate products,had the highest A prediction accuracy compared with other candidates;and 3)for samples where LSTM(Q_(in),C_(a),T_(air))had poor prediction accuracy,BALSTMC_(i),g_(sw)(Q_(in),C_(a),T_(air))clearly improved the ***,it was found that incorrect predictions may be formed when certain factors are not reflected in the data(e.g.,timing,cultivar,and growth stage)or when the training data distribution that accounts for these factors differs from the predicted data ***,a robust model should be constructed in the future to improve the prediction accuracy of A by conducting gasexchange measurements(including a wide range of external environmental values)and by increasing the

关键词： Hybrid prediction model assimilation rate leaf internal variables recurrent neural network fluctuating light environments rice

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Phosphorus Changes and Sorption Characteristics in a Calcareous Soil Under Long-Term Fertilization

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Pedosphere 2008年第2期18卷 248-256页

作者： GUO Sheng-Li DANG Ting-Hui HAO Ming-De Northwest Sci- Tech University of agriculture and Forestry Yangling Shaanxi 712100 （China） institute of Soil and Water Conservation Chinese Academy of Sciences and Ministry of Water Resources Yangling Shaanxi 712100 （China）

Knowledge of phosphorus （P） behavior in long-term fertilized soils is essential for programming fertilization practices and for sustaining environmental quality. The long-term （1984-1997） effects of various fertilization treatments on P changes and sorption isotherms as well as the relationship of soil properties to P sorption and P forms were evaluated in an Ustic Isohumisol, a calcareous soil, on the Loess Plateau, China. Compared to 1984, after 13 years of crop production, total soil P in the no-P treatments （control and N treatment） decreased by 5%-7%, but in the phosphorus fertilizer alone （P）, nitrogen and phosphorus fertilizers in combination （NP）, manure alone （M）, and nitrogen and phosphorus fertilizers and manure in combination （NPM） treatments, it increased by 22%, 19%, 28%, and 58%, respectively. Residual fertilizer P was found mainly in NH4Ac-soluble P （Cas-P）, followed by NaHCO3-soluble P （NaHCO3-P）, and NH4F-soluble P （Al-P）. Phosphorus sorption in the soils with different fertilization practices fit the Langmuir equations. Phosphorus sorption capacity in the no-P treatments increased, whereas it decreased in the P-included treatments （P, NP, and NPM treatments）. Phosphorus sorption maximum （Qm） was significantly and negatively correlated to inorganic P including NaHCO3-P, Cas-P, NaOH-Na2CO3-soluble P （Fe-P）, and Al-P （P ≤ 0.01）. Moreover, long-term fertilization increased soil organic carbon in the NP, M, and NPM treatments and decreased pH in the NP and NPM treatments. Thus, the ability of the soil to release sorbed P to the environment increased under long-term P fertilization.

关键词： calcareous soil long-term fertilization P accumulation P sorption P transformations

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Research on Vibration Mechanism of Plant Cell Membrane with Ultrasonic Irradiation

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Chinese Physics Letters 2015年第3期32卷 63-65页

作者：曹辉黄婉君乔佳婷王云鹏赵海君 shaanxi Key Laboratory of Ultrasound Institute of AcousticsShaanxi Normal University

The vibration mechanism of the cell membrane of plant seeds is analyzed based on the physical effect of ultrasound. The vibration model of cell rectangular membrane of plant seeds is then proposed and the expression of resonance frequency is obtained. The resonance frequency of cell membrane under the built model in the case of knowing the surface tension, surface density and size of cell membrane are calculated. The results of the theoretical calculations are in accordance with the previous experimental results. The built vibration model of cell membrane has certain rationality and certain reference significance to the determination of parameters in ultrasonic breeding in physical agriculture.

关键词： PLANT cell membranes RESEARCH SEEDS -- Irradiation PERMEABILITY ULTRASONIC transducers RESONANCE frequency analysis

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Transcriptome response of wheat Norin 10 to long-term elevated CO_2 under high yield field condition

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Journal of Integrative agriculture 2016年第9期15卷 2142-2152页

作者： LIN Yue-bing SHEN Cheng-guo LIN Er-da HAO Xing-yu HAN Xue institute of Environment and Sustainable Development in agriculture Chinese Academy of Agricultural Sciences/Key Laboratory of Agricultural Environment Ministry of Agriculture bioinformatics Unit Source Bio Science College of Agronomy Shanxi Agricultural University

The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO_2 in comparison with ambient CO_2. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity（without a reference genome） resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO_2 concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO_2（550 μmol mol^–1） in contrast to that at ambient CO_2（400 μmol mol^–1）; these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO_2 during long-term period in free air CO_2 enrichment（FACE） on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.

关键词： Norin 10 transcriptome response wheat long term elevated carbon dioxide GO enrichment

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Bubble nucleation in spherical liquid cavity wrapped by elastic medium

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Chinese Physics B 2023年第6期32卷 323-332页

作者：张先梅李凡王成会胡静莫润阳沈壮志郭建中林书玉 institute of shaanxi Key Laboratory of Ultrasonics Shaanxi Normal UniversityXi'an 710119China

According to classical nucleation theory, gas nuclei can generate and grow into a cavitation bubble when the liquid pressure exceeds a threshold. However, classical nucleation theory does not include boundary effects. An enclosed spherical liquid cavity surrounded by elastic medium is introduced to model the nucleation process in tissue. Based on the equilibrium pressure relationship of a quasi-static process, the expressions of the threshold and the modified nucleation rate are derived by considering the tissue elasticity. It is shown that the constraint plays an important role in the nucleation process. There is a positive correlation between nucleation threshold pressure and constraint, which can be enhanced by an increasing tissue elasticity and reducing the size of the cavity. Meanwhile, temperature is found to be a key parameter of nucleation process, and cavitation is more likely to occur in confined liquids at temperature T > 100℃. In contrast, less influences are induced by these factors, such as bulk modulus, liquid cavity size, and acoustic frequency. Although these theoretical predictions of the thresholds have been demonstrated by many previous researches, much lower thresholds can be obtained in liquids containing dissolved gases, e.g., the nucleation threshold is about-21 MPa in a liquid of 0.8-nm gas nuclei at room temperature. Moreover, when there is a gas nucleus of 20 nm, the theoretical threshold pressure might be less than1 MPa.

关键词： elastic confinement liquid cavities cavitation nucleation

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Amine-functionalized hierarchically porous carbon supported Pd nanocatalysts for highly efficient H2 generation from formic acid with fast-diffusion channels

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Journal of Energy Chemistry 2023年第1期76卷 249-258,I0007页

作者： Xianzhao Shao Xinyi Miao Fengwu Tian Miaomiao Bai Xiaosha Guo Wei Wang Zuoping Zhao Xiaohui Ji Miyi Li Fangan Deng shaanxi Key Laboratory of Catalysis School of Chemistry and Environment ScienceShanxi University of TechnologyHanzhong 723001ShaanxiChina School of Mathematics and Computer Science Shanxi University of TechnologyHanzhong 723001ShaanxiChina State Key Laboratory of Qinba bio-Resource and Ecological Environment Shaanxi University of TechnologyHanzhong 723001ShaanxiChina School of Chemistry and Chemical Engineering Beijing Institute of TechnologyBeijing 102488China

Formic acid(FA)has come to be considered a potential candidate for hydrogen storage,and the development of efficient catalysts for H2releasing is crucial for realizing the sustainable process from ***,we have developed the ultrafine Pd nanoparticle(NPs)with amine-functionalized carbon as a support,which was found to show an excellent catalytic activity in H_(2)generation from FA *** synergetic mechanism between amine-group and Pd active site was demonstrated to facilitate H2generation byβ-hydride ***,the texture of support for Pd NPs also plays an important role in determining the reactivity of FA,since the diffusion of gaseous products makes the kinetics of diffusion as a challenge in this high performance Pd *** a result,the as-prepared Pd/NH_(2)-TPC catalyst with the small sized Pd nanoparticles and the hierarchically porous structures shows a turnover of frequency(TOF)value of 4312 h^(-1)for the additive-free FA dehydrogenation at room temperature,which is comparable to the most promising heterogeneous *** results demonstrated that the intrinsic catalytic activities of active site as well as the porous structure of support are both important factors in determining catalytic performances in H2generation from FA dehydrogenation,which is also helpful to develop high-activity catalysts for other advanced gas-liquid-solid reactions systems.

关键词： Hierarchically carbon Diffusion Formic acid Hydrogen production Palladium nanoparticles

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Insight Into the Separation-of-Variable Methods for the Closed-Form Solutions of Free Vibration of Rectangular Thin Plates

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 329-355页

作者： Yufeng Xing Ye Yuan Gen Li institute of Solid Mechanics Beihang University(BUAA)Beijing100191China

The separation-of-variable(SOV)methods,such as the improved SOV method,the variational SOV method,and the extended SOV method,have been proposed by the present authors and coworkers to obtain the closed-form analytical solutions for free vibration and eigenbuckling of rectangular plates and circular cylindrical *** taking the free vibration of rectangular thin plates as an example,this work presents the theoretical framework of the SOV methods in an instructive way,and the bisection–based solution procedures for a group of nonlinear eigenvalue ***,the explicit equations of nodal lines of the SOV methods are presented,and the relations of nodal line patterns and frequency orders are *** is concluded that the highly accurate SOV methods have the same accuracy for all frequencies,the mode shapes about repeated frequencies can also be precisely captured,and the SOV methods do not have the problem of missing roots as well.

关键词： Separation-of-variable method Rayleigh quotient nodal line eigenvalue equation bisection method

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