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Transcriptomic Analysis Reveals the Formation Mechanism of Anthocyanins Light-Independent Synthesis in Chrysanthemum

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Phyton-International Journal of Experimental Botany 2024年第7期93卷 1599-1621页

作者： Fangye Liu Jiaping Qu Yajun Li Jiawei Fan Yumeng Cui Jingwen Wu Xueqi Gu Xia Li He Huang beijing advanced innovation Center for tree breeding by molecular design Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Education MinistrySchool of Landscape ArchitectureBeijing Forestry UniversityBeijing100083China Urban Construction School Beijing City UniversityBeijing100083China

Chrysanthemum×morifolium is a horticultural crop which plays a vital role in theﬂower industry with signiﬁ-cant economic value and has a cultivation history of over three thousand years in *** accumulation of anthocyanins is always affected by ***,we revealed that anthocyanin accumulation is highly dependent on light in‘2021135’genotype chrysanthemum,while it is light-independent in‘2001402’genotype ***,no literature has been reported regarding the non-photosensitive chrysanthemum in anthocya-nins light-independent synthesis *** the phenotype analysis of 44 F1 generations,we found that light-independence is a dominant trait which can be stable inherited by *** transcriptome of the rayﬂorets of‘2021135’and‘2001402’under light and bagging treatment were sequenced and *** on weighted gene co-expression network analysis(WGCNA),K-means analysis,and Real-Time Quantitative Poly-merase Chain Reaction(RT-qPCR)analysis,16 genes were highly correlated with the anthocyanin *** anthocyanin content of rayﬂorets treated with different light-quality conditions indicated that blue light signiﬁ-cantly affected anthocyanin *** Yeast one-hybrid analysis,CmBIC1.1 and CmBIC1.2 can directly regulate the anthocyanin structural gene *** our study,we revealed the important characteristics of light-independent anthocyanin synthesis in chrysanthemums and screened regulatory factors in light-depen-dent and light-independent anthocyanin synthesis *** results laid the groundwork for subsequent ana-lysis of the molecular mechanism involved in the light-independent synthesis of anthocyanins in chrysanthemums.

关键词： Chrysanthemum non-photosensitive transcriptome anthocyanin biosynthesis

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Selection of female parents with high fertility and high combining abilities for cross-breeding Populus tomentosa

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Journal of Forestry Research 2019年第2期30卷 445-450页

作者： Feng-ying Bai Ning Kang Ping-dong Zhang Xiang-yang Kang National Engineering Laboratory for tree breeding Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Laboratory of Urban and Rural Ecological Environment Beijing Forestry University

To select maternal parents with high fertility and high combining ability during cross breeding of Populus tomentosa Carr., the ploidy level of 75 female clones was determined using flow cytometry. In addition, genetic variations and phenotypic correlations of seed traits and early growth traits, as well as the general combining ability(GCA) for seedling height(SH) and basal diameter(BD) of all diploid half-sib families were examined. A total of 26 natural triploid clones were identified. For all diploid families, family effects were significant for inflorescence length(IL), thousand kernel weight(TKW), seedling emergence rate(SER), and for SH and BD. There was a positive correlation between TKW and other traits,excluding IL. The seed-bearing coefficient(NS 9 SER)ranged from zero to 32.4%. Clones 3-10-2, 2-8, 3119,3206, and 3-10-1 had the best performance of the GCA for SH and BD. Based on the fertility and GCA for SH and BD, clone 3-10-2 and clone 3119 could be used as female parents for controlled cross-breeding of P. tomentosa.

关键词： Fertility ability General combining ability Genetic variation Half-sib family Populus tomentosa

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Phylogenetic relationship of Picea mongolica with other Picea species in the same area based on chloroplast gene variations

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Journal of Forestry Research 2021年第1期32卷 297-305页

作者： Hao Di Jingjing Ma Kuaikuai He Fangxu Han Yue Li Shihui Niu beijing advanced innovation Center for tree breeding by molecular design National Engineering Laboratory for Tree BreedingCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing 100083People’s Republic of China

Picea mongolica is a conifer with a limited distribution in China and its taxonomic status is *** order to explore genetic diff erences between *** and other nearby Picea species and to investigate its taxonomic status,phylogenetic relationships were analyzed between *** and Picea koraiensis,Picea meyeri and Picea wilsonii by three chloroplast gene sequences matK,chlB and *** length of joint chloroplast sequence is 2379 *** fi fteen haplotypes were identifi ed by haplotype network analysis,among which two were major haplotypes and nine were *** addition,the genetic diversity of the sample collection species was *** on the haplotype network and Neighbor Joining phylogenetic tree analysis,*** was located on the basal clade of the phylogenetic tree which had more primitive taxa,and the genetic diversity of *** was *** signifi cant diff erences between *** and these other Picea species were identifi ed by this research.

关键词： cpDNA Genetic diff erence Phylogeny Picea mongolica Taxonomic status

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Identification, through transcriptome analysis, of transcription factors that regulate anthocyanin biosynthesis in different parts of red-fleshed apple 'May' fruit

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Horticultural Plant Journal 2022年第1期8卷 11-21页

作者： Hua Li Shiyao Duan Weilei Sun Sifan Wang Jie Zhang Tingting Song Ji Tian Yuncong Yao beijing advanced innovation Center for tree breeding by molecular design Beijing University of AgricultureBeijing 102206China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China

Anthocyanins, a class of flavonoid compounds that provide pigmentation to plant organs, are beneficial components of the human diet and there is an interest in understanding the regulation of their formation in crop plants. The apple cultivar ‘May’ represents an excellent model for the study of anthocyanin metabolism due to the spatial color pattern of its flesh, which transitions sequentially from red to white to red again,from the outside to the inside of the apple fruit. To screen the transcription factors involved in the anthocyanin pathway, transcriptomes from differently colored sections of ‘May’ fruits were analyzed by RNA sequencing. Kyoto Encyclopedia of Genes and Genomes(KEGG) database was used to analyse the gene expression data. The results suggested differentially expressed genes(DEGs) that are related to phytohormones are involved in regulating anthocyanin biosynthesis. K-means clustering analysis revealed 167 common DEGs between different sections of fruit with the same expression pattern as candidates for regulating anthocyanin synthesis. Further analysis showed that nine of the 167 DEGs were annotated as transcription factors and quantitative real time PCR(qRT-PCR) confirmed that their expression was obviously higher in red regions of the fruit, consistent with their roles as hub genes that regulate anthocyanin synthesis. This study provides valuable results for future studies of anthocyanin synthesis in apple fruit.

关键词： Anthocyanins Apple fruit Red flesh RNA sequencing Hormones

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Tobacco Rattle Virus-induced Phytoene Desaturase (PDS) Silencing in Centaurea cyanus

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Horticultural Plant Journal 2021年第2期7卷 159-166页

作者： Chengyan Deng Fan Zhang Jiaying Wang Yanfei Li He Huang Silan Dai beijing Key Laboratory of Ornamental Plants Germplasm innovation&molecular breeding National Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological Environment and College of Landscape ArchitectureBeijing Advanced Innovation Center for Tree Breeding by Molecular DesignBeijing Forestry UniversityBeijing 100083China

Virus-induced gene silencing(VIGS)is a genetic tool used to assess gene *** rattle virus(TRV)is a VIGS vector commonly used to induce endogenous gene silencing in ***,there is no VIGS system established for Centaurea *** evaluated the effectiveness of a TRV-based VIGS system using phytoene desaturase(PDS)as a reporter gene in Centaurea *** methods including pressure-,vacuum-and apical meristem-infiltrationwere tested to infect *** *** leaveswere only obtained using apicalmeristem-infiltration after a 14 d *** CcPDS transcripts in photobleached leaves were significantly reduced compared with that in green leaves treated with empty *** *** cultivars were tested to detect their VIGS responses,and‘Dwarf Tom Pouce Blue’was the most *** agro-infiltration condition was optimized by screening for the optimal seedling stage as well as the optimum Agrobacterium density for efficient *** with four true leaves and infiltration with an Agrobacterium density of OD_(600)0.5 were optimal conditions to obtain more photobleached leaves and more intense photobleached *** results demonstrated the feasibility of TRV-based VIGS for functional analysis of genes in ***.

关键词： Centaurea cyanus VIGS tobacco rattle virus PDS seedling photobleached

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Chromosome doubling mediates superior drought tolerance in Lycium ruthenicum via abscisic acid signaling

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Horticulture Research 2020年第1期7卷 2199-2216页

作者： Shupei Rao Yuru Tian Xinli Xia Yue Li Jinhuan Chen beijing advanced innovation Center for tree breeding by molecular design Beijing Forestry University100083 BeijingChina College of Biological Sciences and Technology Beijing Forestry University100083 BeijingChina National Engineering Laboratory for tree breeding Beijing Forestry University100083 BeijingChina

Plants are continuously affected by unfavorable external stimuli,which influences their productivity and *** in gene composition and expression patterns lead homologous polyploid plants to exhibit different physiological phenomena,among which enhanced environmental adaptability is a powerful phenotype conferred by *** mechanisms underlying the differences in stress tolerance between diploids and autotetraploids at the molecular level remain *** this research,a full-length transcription profile obtained via the singlemolecule real-time(SMRT)sequencing of high-quality single RNA molecules for use as background was combined with next-generation transcriptome and proteome technologies to probe the variation in the molecular mechanisms of *** exhibited an increase in ABA content of 78.4%under natural conditions and a superior stress-resistance phenotype under severe drought stress compared with *** substantial differences in the transcriptome profiles observed between diploids and autotetraploids under normal growth conditions were mainly related to ABA biosynthesis and signal transduction pathways,and 9-cis-epoxycarotenoid dioxygenase 1(NCED1)and NCED2,which encode key synthetic enzymes,were significantly *** increased expression of the ABREbinding factor 5-like(ABF5-like)gene was a pivotal factor in promoting the activation of the ABA signaling pathway and downstream target *** addition,ABA strongly induced the expression of osmotic proteins to increase the stress tolerance of the plants at the translational *** consider the intrinsic mechanisms by which ABA affects drought resistance in tetraploids and diploids to understand the physiological and molecular mechanisms that enhance abiotic stress tolerance in polyploid plants.

关键词： drought resistance media

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Genome-wide identification and analysis of the DREB genes and their expression profiles under abiotic stresses in Chinese jujube(Ziziphus jujuba Mill.)

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Journal of Forestry Research 2019年第4期30卷 1277-1287页

作者： Heying Zhou Jiping Jia Decang Kong Zhendong Zhang Shuang Song Yingyue Li Xiaoming Pang beijing advanced innovation Center for tree breeding by molecular design National Engineering Laboratory for Tree BreedingKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental PlantsMinistry of EducationCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China National Foundation for Improved Cultivar of Chinese Jujube Cangzhou 061000HebeiChina

CBF/DREB proteins play a critical role in abiotic stress-mediated gene expression and represent attractive regulons for plant breeding ***,no study has been conducted for CBF/DREB protein-related genes in jujube(Ziziphus jujuba Mill.).In this study,twenty-five ZjDREB genes were identified and annotated from the jujube(***‘Dongzao’)*** analysis,including gene classification,annotation,phylogenetic evaluation,conserved motif determination and expression profiling were performed on all *** analysis showed that ZjDREB proteins were divided into five subgroups(A1–A5),but lacking a subgroup A6 corresponding to *** ZjDREB genes were distributed in nine of twelve chromosomes in the ***,the expression patterns of the DREB genes under different abiotic stresses were investigated using q *** ZjDREB genes were down-regulated under low temperature,in contrast six ZjDREB genes(01,03,05,11,23 and 24)were *** drought,salinity and high temperature conditions,expression of ZjDREB03,09,10,14,15,17 and 20 genes were induced and showed similar expression patterns,suggesting that various stress conditions share common elements in the signaling *** results suggest that the family of DREB genes play an important role in abiotic stresses in jujube,and provide a foundation for further functional studies of this important class of transcriptional regulators.

关键词： Abiotic stress Chinese jujube DREB Expression patterns Phylogenetic analysis

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Metabolome profiling of stratified seeds provides insight into the regulation of dormancy in Davidia involucrata

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Plant Diversity 2022年第4期44卷 417-427页

作者： Shiming Deng Qiang Xiao Cigui Xu Jian Hong Zhijun Deng Dan Jiang Shijia Luo Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province Hubei Minzu UniversityEnshi 445000Hubei ProvinceChina beijing advanced innovation Center for tree breeding by molecular design National Engineering Laboratory for Tree BreedingCollege of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijing 100083China

Dove tree(Davidia involucrata),a tertiary vestige species,is well-adapted to cool *** in *** seed lasts for an extremely long period of time,typically between 3 and 4 years,and this characteristic makes the species an excellent model for studying the mechanisms of seed *** molecular mechanisms governing germination control in *** are still *** stratification have been reported to enhance germination in recalcitrant *** performed a widely targeted metabolome profiling to identify metabolites and associated pathways in *** seeds from six different moist sand stratification durations(0-30 months) using the ultra-high-performance liquid chromatography-Q Exactive Orbitrap-Mass *** was an increasing germination rate with prolonged stratification durations(12-30 months).Furthermore,we detected 10,008 metabolites in the stratified *** also detected 48 differentially accumulated metabolites(DAMs) between all stratification periods in the seeds,with 10 highly conserved *** of the differentially accumulated metabolites between unstratified and stratified seeds were enriched in purine metabolism,pyrimidine metabolism,flavone and flavonol biosynthesis,phenylpropanoid biosynthesis,and arginine biosynthesis *** phytohormones,abscisic acid,indole-3 acetic acid,and sinapic acid were differentially accumulated in the seeds and are predicted to regulate dormancy in *** have provided extensive metabolic information useful for future works on dove tree germination study.

关键词： Dove tree Metabolome Dormancy Abscisic acid Stratification

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Improving sample preparation to investigate lignin intensity of xylem at the cellular level by confocal Raman microspectroscopy of Populus tomentosa

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Journal of Forestry Research 2021年第5期32卷 2135-2142页

作者： Bing Wang Mei Luo Yadi Liu Xiaorui Guo Xiatong Liu Chong Zhang Zhijing Zhao Di Liu Hui Li Hai Lu beijing advanced innovation Center for tree breeding By molecular design Beijing Forestry UniversityBeijing 100083People’s Republic of China College of Biological Sciences and Biotechnology Beijing Forestry UniversityBeijing 100083People’s Republic of China National Engineering Laboratory for tree breeding Beijing Forestry UniversityBeijing 100083People’s Republic of China

Confocal Raman microspectroscopy(CRM)is an important tool for analyzing the compositional distribution of cell walls in *** this study,we improved the sample preparation method using paraffin-embedded sections combined with hexane dewaxing to obtain high resolution Raman *** determined that the cell wall components of fiber cells were different from those of ray cells and vessel cells in the xylem of Populus *** bromide and CRM methods produced similar trends when the difference in lignin intensity in the xylem region was compared between transgenic PtrLac4 and wild-type ***,CRM proved more useful to analyze the lignin distribution in each cell type and distinguished the detailed difference in lignin intensity at the cellular ***,CRM proved to be a useful in situ method to rapidly analyze the spatial variation of lignin content in the xylem of woody plants.

关键词： Cell wall Confocal Raman microspectroscopy Lignin Paraffin section Populus tomentosa

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Sorbitol induces flower bud formation via the MADS-box transcription factor EjCAL in loquat

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Journal of Integrative Plant Biology 2023年第5期65卷 1241-1261页

作者： Hong-Xia Xu Dong Meng Qing Yang Ting Chen Meng Qi Xiao-Ying Li Hang Ge Jun-Wei Chen Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhou 310021China beijing advanced innovation Center for tree breeding by molecular design Beijing Forestry UniversityBeijing 100000China College of Forestry Beijing Forestry UniversityBeijing 100083China

Sorbitol is an important signaling molecule in fruit trees. Here, we observed that sorbitol increased during flower bud differentiation(FBD) in loquat(Eriobotrya japonica Lindl.). Transcriptomic analysis suggested that bud formation was associated with the expression of the MADS-box transcription factor(TF) family gene, EjCAL. RNA fluorescence in situ hybridization showed that EjCAL was enriched in flower primordia but hardly detected in the shoot apical meristem. Heterologous expression of EjCAL in Nicotiana benthamiana plants resulted in early FBD. Yeast-one-hybrid analysis identified the ERF12 TF as a binding partner of the EjCAL promoter. Chromatin immunoprecipitation-PCR confirmed that EjERF12 binds to the EjCAL promoter, and β-glucuronidase activity assays indicated that EjERF12 regulates EjCAL *** loquat trees with sorbitol promoted flower bud formation and was associated with increased expression of EjERF12 and EjCAL. Furthermore, we identified EjUF3GaT1 as a target gene of EjCAL and its expression was activated by EjCAL. Function characterization via overexpression and RNAi reveals that EjUF3GaT1 is a biosynthetic gene of flavonoid hyperoside. The concentration of the flavonoid hyperoside mirrored that of sorbitol during FBD and exogenous hyperoside treatment also promoted loquat bud formation. We identified a mechanism whereby EjCAL might regulate hyperoside biosynthesis and confirmed the involvement of EjCAL in flower bud formation in planta. Together,these results provide insight into bud formation in loquat and may be used in efforts to increase yield.

关键词： EjCAL flower bud differentiation hyperoside loquat sorbitol

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