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Chinese Journal of Natural Medicines 2022年第8期20卷 633-640页

作者： QIN Chun-Jun HU Jing TONG Wei ZHANG Teng-Teng TIAN Guang-Zong ZoU Xiao-Peng LIU Jian-Kai YIN Jian Key Laboratory of Carbohydrate Chemistry and biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China National&Local Joint Engineering Laboratory for Novel Vaccine Development Technology Beijing Minhai Biotechnology Co.Ltd.Beijing 102600China Jiangnan University-beijing minhai biotechnology Joint Research Center of Carbohydrate-Based Vaccines Jiangnan UniversityWuxi 214122China

The ribose and phosphorus co.tents in Haemophilus influenzae type b(Hib)capsular polysaccharide(CPS)are two important chemical indexes for the development and quality co.trol of Hib co.jugate vaccine.A quantitative ^(1)H-and ^(31)P-NMR method using a single internal standard was developed for simultaneous determination of ribose and phosphorus co.tents in Hib ***(HMPA)was successfully utilized as an internal standard in quantitative ^(1)H-NMR method for ribose co.tent *** ribose and phosphorus co.tents were found to be affected by the co.centration of polysaccharide ***,15–20 mg·L^(−1) was the optimal co.centration range of Hib CPS in D_(2)O solution for determination of ribose and phosphorus co.tents by this *** ribose and phosphorus co.tents obtained by the quantitative NMR were co.sistent with those obtained by traditional chemical *** co.clusion,this quantitative ^(1)H-and ^(31)P-NMR method using a single internal standard shows good specificity,accuracy and precision,providing a valuable approach for the quality co.trol of Hib glyco.onjugate vaccines.

关键词： Haemophilus influenzae type b Capsular polysaccharide Quantitative^(1)H-NMR Quantitative^(31)P-NMR Ribose co.tent Phosphorus co.tent

Biosynthesis and Immunological Evaluation of a Dual-Antigen Nanoco.jugate Vaccine Against Brucella melitensis

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Engineering 2023年第10期29卷 95-109页

作者： Jing Huang Yufei Wang Kangfeng Wang Shulei Li Peng Sun Yan Guo Jiankai Liu Ruifu Yang Ming Zeng Chao Pan Hengliang Wang Li Zhu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of BiotechnologyBeijing 100071China beijing minhai biotechnology co. Ltd.Beijing 102600China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China The Third Medical Center PLA General HospitalBeijing 100039China co.lege of Life Science Hebei UniversityBaoding 071002China

Brucellosis,caused by Brucella,is one of the most co.mon ***,there is still no vaccine for human *** some live attenuated vaccines have been approved for animals,the protection effect is not *** this study,we developed a dual-antigen nanoco.jugate vaccine co.taining both polysaccharide and protein antigens against ***,the antigenic polysaccharide was co.alently co.pled to the outer membrane protein Omp19 using protein glycan co.pling technology,and then it was successfully loaded on a nano-carrier through the SpyTag/SpyCatcher *** co.firming the efficient immune activation and safety performance of the dual-antigen nanoco.jugate vaccine,the potent serum antibody response against the two antigens and remarkable protective effect in non-lethal and lethal Brucella infection models were further demonstrated through different routes of *** results indicated that the dual-antigen nanoco.jugate vaccine enhanced both T helper 1 cell(Th1)and Th2 immune responses and protected mice from Brucella ***,we found that this protective effect was maintained for at least 18 *** our knowledge,this is the first Brucella vaccine bearing diverse antigens,including a protein and polysaccharide,on a single ***,we also present an attractive technology for co.delivery of different types of antigens using a strategy applicable to other vaccines against infectious diseases.

关键词： Protein glycan co.pling technology(PGCT) Dual-antigen Nanoco.jugate vaccine Brucella melitensis

Development of an Integrated Disposable Device for SARSco.-2 Nucleic Acid Extraction and Detection

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Biomedical and Environmental Sciences 2024年第6期37卷 639-646页

作者： Jing Ma Yanzhe Hao Meiling Hou Xiaoshan Zhang Jingduan Liu Haodi Meng Jiangbo Chang Xuejun Ma Jihua Liu Qingjie Ying Xianhua Wang Hongxia Li Yuxi Cao Xiaoguang Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China beijing Runbio biotechnology Development co. LtdBeijing 100106China beijing Beta Technology co. LtdBeijing 101318China Jiangsu Qitian Gene biotechnology co. LtdWuxi 214135JiangsuChina

Objective To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome co.onavirus 2(SARS-co.-2).Methods We designed,developed,and manufactured an integrated disposable device for SARS-co.-2 nucleic acid extraction and *** precision of the liquid transfer and temperature co.trol was tested.A co.parison between our device and a co.mercial kit for SARS-co.-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).The entire process,from SARS-co.-2 nucleic acid extraction to amplification,was *** The precision of the syringe transfer volume was 19.2±1.9μL(set value was 20),32.2±1.6(set value was 30),and 57.2±3.5(set value was 60).Temperature co.trol in the amplification tube was measured at 60.0±0.0℃(set value was 60)and 95.1±0.2℃(set value was 95)***-co.-2 nucleic acid extraction yield through the device was 7.10×10^(6) co.ies/mL,while a co.mercial kit yielded 2.98×10^(6) co.ies/*** mean time to co.plete the entire assay,from SARS-co.-2 nucleic acid extraction to amplification detection,was 36 min and 45 *** detection limit for SARS-co.-2 nucleic acid was 250 co.ies/*** The integrated disposable devices may be used for SARS-co.-2 Point-of-Care test(POCT).

关键词： An integrated disposable device SARS-co.-2 Nucleic acid detection

A neural network-based production process modeling and variable importance analysis approach in co.n to sugar factory

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Frontiers of Chemical Science and Engineering 2023年第3期17卷 358-371页

作者： Yi Tong Mou Shu Mingxin Li Yingwei Liu Ran Tao co.gco.g Zhou You Zhao Guoxing Zhao Yi Li Yachao Dong Lei Zhang Linlin Liu Jian Du co.co.biotechnology co. Ltd.Beijing 100005China co.co.Nutrition and Health Research Institute co. Ltd.Beijing 102209China Institute of Process Systems Engineering School of Chemical EngineeringDalian University of TechnologyDalian 116024China

co.n to sugar process has long faced the risks of high energy co.sumption and thin ***,it’s hard to upgrade or optimize the process based on mechanism unit operation models due to the high co.plexity of the related *** data technology provides a promising solution as its ability to turn huge amounts of data into insights for operational *** this paper,a neural network-based production process modeling and variable importance analysis approach is proposed for co.n to sugar processes,which co.tains data preprocessing,dimensionality reduction,multilayer perceptron/co.volutional neural network/recurrent neural network based modeling and extended weights co.nection *** the established model,dextrose equivalent value is selected as the output,and 654 sites from the DCS system are selected as the *** analysis is first applied to reduce the data dimension to 155,then the inputs are dimensionalized to 50 by means of genetic algorithm ***,variable importance analysis is carried out by the extended weight co.nection method,and 20 of the most important sites are selected for each neural *** results indicate that the multilayer perceptron and recurrent neural network models have a relative error of less than 0.1%,which have a better prediction result than other models,and the 20 most important sites selected have better explicable *** major co.tributions derived from this work are of significant aid in process simulation model with high accuracy and process optimization based on the selected most important sites to maintain high quality and stable production for co.n to sugar processes.

关键词： big data co.n to sugar factory neural network variable importance analysis

Synthesis and Characterization of Novel Biodegradable Poly (butylene succinate-co.triethylene glyco. succinate)co.olymers

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Journal of beijing Institute of Technology 2017年第2期26卷 276-284页

作者： Jie Ouyang Hongquan Yin Qingshan Zhang Yunzheng Li Pengjun Yao . School of Chemical Engineering and Environment Beijing Institute of Technology Beijing 100081 China Anhui Sealong biotechnology co. Ltd Bengbu Anhui 233316 China

Novel biodegradable aliphatic polyesters,poly（butylene succinate-co.triethylene glyco. succinate）（P（BS-co.TEGS））and poly（butylene succinate）（PBS）,were synthesized through a two-step procedure of esterification and polyco.densation with succinic acid and1,4-butanediol/triethylene glyco. as raw materials as well as tetrabutyl titanate and diphenylphosphinic acid as the *** chemical structure and molecular weight of the co.olymers were characterized by 1^H nuclear magnetic resonance（1^H NMR）and gel permeation chromatography（GPC）,*** addition,thermal properties,crystal structure and mechanical properties were also analyzed with various techniques.P（BS-co.TEGS）exhibited more excellent mechanical properties than PBS,especially in elongation at ***,the crystal structure and thermal stability of the P（BS-co.TEGS）have hardly *** crystallinity of P（BS-co.TEGS）was lower than that of PBS and decreased with the increase of mole ratio of triethylene *** the increase of TEGS unit molar co.position,the melting point（Tm）,crystallization temperatures（Tc）and heat of fusion（ΔHm）of P（BS-co.TEGS）decreased,while glass transition temperature（Tg）increased.

关键词： aliphatic polyesters polyco.desation mechanical properties crystallinity

Mannanase improves the growth performance of broilers by alleviating inflammation of the intestinal epithelium and improving intestinal microbiota

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Animal Nutrition 2024年第1期16卷 376-394页

作者： Xiaodan Zhang Huiping Xu Lu Gong Jiao Wang Jianyang Fu Zengpeng Lv Liangjuan Zhou Xuejun Li Qiong Liu Pingyu Xia Yuming Guo State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China beijing Strowin biotechnology co. Ltd.Beijing100094China

This experiment aimed to discuss and reveal the effect and mechanism of mannanase on intestinal inflammation in broilers triggered by a soybean meal *** this experiment,384 Arbor Acres broilers at1 d old were randomly divided into 3 treatment *** broilers were fed a co.n-soybean meal basal diet,a low-energy diet(metabolizable energy reduced by 50 kcal/kg),and a low-energy diet supplemented with 100 mg/kg mannanase for 42 *** low-energy diet increased feed co.version ratio from0 to 42 d,reduced ileal villus height and villus height-to-crypt depth ratio and upregulated the expression of nuclear factor kappa B(NF-κB)in the ileum(Pco.pared with low-energy diets,the addition of mannanase increased body weight at 42 d,promoted the digestibility of nutrients,and maintained the morphology and integrity of the intestinal epithelium of broilers(Pco.tent of isobutyric acid in the cecum of broilers(Pco.cus in the intestine of broilers and inhibited oxidative phosphorylation and phosphoinositol *** increased the relative abundance of Odoribacter,promoted energy metabolism and N-glycan biosynthesis,and increased the activities of GH3 and *** is co.cluded that mannanase co.ld improve the growth performance of broilers by reducing the expression of NF-κB in the ileum,increasing the production of SCFA in the cecum,suppressin

关键词： Broiler Low-energy diet Mannanase Intestinal inflammatory response Intestinal microbiota Metagenome

Aberrant histone H4 acetylation in dead somatic cell-cloned calves

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Progress in Natural Science:Materials International 2008年第7期18卷 813-818页

作者： Lei Zhang a,b,*, Shaohua Wang a, Qiang Li a, Xiangdong Ding c, Yunping Dai a, Ning Li a,* a State Key Laboratory for Agrobiotechnology, China Agricultural University, beijing 100094, China b beijing Gene & Protein biotechnology co. LTD, beijing 100094, China c co.lege of Animal Science and Technology, China Agricultural University, beijing 100094, China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China beijing Gene & Protein biotechnology co. LTD Beijing 100094 China co.lege of Animal Science and Technology China Agricultural University Beijing 100094 China

In somatic cell-cloned animals, inefficient epigenetic reprogramming can result in an inappropriate gene expression and histone H4 acetylation is one of the key epigenetic modifications regulating gene expression. In this study, we investigated the levels of histone H4 acetylation of 11 development-related genes and expression levels of 19 genes in lungs of three normal co.trol calves and nine aber- rant somatic cell-cloned calves. The results showed that nine studied genes had decreased acetylation levels in aberrant clones (p 0.05). Whereas 13 genes had significantly decreased expression (p co.trols and clones (p > 0.05), and only one gene had higher expression level in clones (p co.trols, and the level of histone H4 acetylation was even more lowered in aberrant clones than those in co.trols. It was suggested that the lower levels of histone H4 acetylation in aberrant clones caused by the previous memory of cell differentiation might not support enough chromatin reprogramming, thus affecting appropriate gene expressions, and growth and development of the cloned calves. To our knowledge, this is the first study on how histone H4 acetylation affects gene expression in organs of somatic cell-cloned calves.

关键词： Somatic cell-cloned calves Histone H4 acetylation Gene expression Lungs

A New Method for Ultra-sensitive P24 Antigen Assay Based on Near-infrared Fluorescent Microsphere Immunochromatography

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Biomedical and Environmental Sciences 2020年第3期33卷 174-182页

作者： WANG Qi HOU Mei Ling LIU Li Peng MA Jing ZHANG Xiao Guang ZHOU Zhi Xiang CAO Yu Xi beijing University of Technology College of Life Science and BioengineeringBeijing 100124China beijing Runbio biotechnology Development co. LtdBeijing 100106China National Institute for Viral Disease co.trol and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective To develop a rapid,highly sensitive quantitative method for detecting P24 antigen based on near-infrared fluorescent microsphere *** First,we prepared a lateral flow assay test strip,and labeled the detection antibody using a fluorescent ***,we optimized the antibody labeling ***,we optimized the detection ***,we created a working ***,we co.ducted a methodological assessment of the established fluorescent microsphere immunochromatography ***-six clinical samples were tested,and we co.pared the established fluorescent microsphere immunochromatography with the quantitative ELISA *** Acco.ding to the working curve,the detection limit of the method is 3.4 pg/mL,and the detection range is 3.4 pg/mL to 10 ng/*** average intra-assay reco.ery was 99.6%,and the co.fficient of Variation(CV)was 5.4%–8.6%;the average inter-assay reco.ery was 97.3%,and the CV was 8.5%–11%.The detection rate of fluorescent microsphere immunochromatography was higher than ELISA method,and had a good co.relation with *** The P24 antigen quantitative detection method based on near-infrared fluorescent microsphere immunochromatography has the advantages of rapid detection,high sensitivity,and wide detection range;thus,it is suitable for early clinical diagnosis and co.tinuous monitoring of AIDS.

关键词： Fluorescent microsphere immunochromatography HIV P24 antigen POCT

Non-small cell lung cancers(NSCLCs)onco.ysis using co.sackievirus B5 and synergistic DNA-damage response inhibitors

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Signal Transduction and Targeted Therapy 2023年第10期8卷 5069-5084页

作者： Bopei Cui Lifang Song Qian Wang Kelei Li Qian He Xing Wu Fan Gao Mingchen Liu Chaoqiang An Qiushuang Gao Chaoying Hu Xiaotian Hao Fangyu Dong Jiuyue Zhou Dong Liu Ziyang Song Xujia Yan Jialu Zhang Yu Bai Qunying Mao Xiaoming Yang Zhenglun Liang Division of Hepatitis and Enterovirus Vaccines NHC Key Laboratory of Research on Quality and Standardization of Biotech ProductsNMPA Key Laboratory for Quality Research and Evaluation of Biological ProductsInstitute of Biological ProductsNational Institutes for Food and Drug ControlBejingChina National Engineering Technology Research Center for co.bined Vaccines WuhanChina beijing minhai biotechnology co LtdBeijingChina Taibang Biologic Group BeijingChina Changchun Institute of Biological Products co. LtdChangchunChina Shanghai Institute of Biological Products co LtdShanghaiChina China National Biotec Group co.pany Limited BeijingChina

With the co.tinuous in-depth study of the interaction mechanism between viruses and hosts,the virus has beco.e a promising tool in cancer *** fact,many onco.ytic viruses with selectivity and effectiveness have been used in cancer *** enterovirus is one of the most co.venient sources to generate onco.ytic viruses,however,the high seroprevalence of some enteroviruses limits its application which urges to exploit more onco.ytic enteroviruses.

关键词： damage enterovirus cancer

Seeing is believing:anti-PD-1/PD-L1 monoclonal antibodies in action for checkpoint blockade tumor immunotherapy

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Signal Transduction and Targeted Therapy 2016年第1期1卷 27-30页

作者： Shuguang Tan Catherine W-H Zhang George F Gao CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina and 2ImmuFuCell Biotechnology Co.Ltd.BeijingChina

Structural immunology,focusing on structures of host immune related molecules,enables the immunologists to see what the molecules look like,and more importantly,how they work ***-based PD-1/PD-L1 blockade therapy has achieved brilliant successes in clinical *** recent breakthrough of the co.plex structures of checkpoint blockade antibodies with their co.nterparts,pembrolizumab with PD-1 and avelumab with PD-L1,have made it clear how these monoclonal antibodies co.pete the binding of PD-1/PD-L1 and function to blockade the receptor-ligand ***,we summarize the structural findings of these two reports and look into the future for how this information would facilitate the development of more efficient PD-1/PD-L1 targeting antibodies,small molecule drugs,and other protein or non-protein inhibitors.

关键词： antibodies monoclonal drugs