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An inhibitor selective for collagen-stimulated platelet aggregation from the salivary glands of hard tick Haemaphysalis longicornis and its mechanism of action

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Science China(Life Sciences) 1999年第5期42卷 457-464页

作者：程远国吴厚永李德昌 institute of microbiology and epidemiology Beijing China

Soluble materials of salivary glands from Haemaphysalis longicornis were found to inhibit collagen, ADP, and thrombin- stimulated platelet aggregation. One inhibitory component was purified to salivary gland homogeneity by a combination of gel filtration, ion-exchange, and C8 reverse phase HPLC. The purified activity, named longicomin, is a protein of molecular weight 16 000 on SDS-PAGE under both reduced and nonreduced conditions. Collagen-mediated aggregation of platelets in plasma and of washed platelets (IC50 was approximately 60 nmol/L) was inhibited with the same efficacy. No inhibition of aggregation stimulated by other effectors, including ADP, arachidonic acid, thrombin, risto-cetin, calcium ionophore A23187, thromboxane A2 mimetic U46619 and 12-0-phorbol-13-myristate acetate, was observed . Longiconin had no effect on platelet adhension to collagen. Not only platelet aggregation but also release reaction, and increase of intracellar Ca2+ level of platelets in response to collagen were completely eliminated by longicomin . Increasing amounts of collagen are able to overcome the inhibition of aggregation by longicomin, indicating that longicomin probably shares with collagen a common receptor. In addition, collagen fibers did not emit fluorescence after incubation with isothocyanate-conjugated longicomin, indicating that longicomin did not bind directly to collagen fibers. The identification and isolation of longicomin demonstrates the existence of a new type of platelet inhibitor that should be useful to better understand the mechanism of collagen stimulation of platelets.

关键词： Haemaphysalis longicornis salivary gland longicornin collagen antiplatelet.

Assessment of the risk of infectious aerosols leaking to the environment from BSL-3 laboratory HEPA air filtration systems using model bacterial aerosols

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Particuology 2014年第2期12卷 82-87页

作者： Zhanbo Wen Wenhui Yang Na Li Jie Wang Lingfei Hu Jinsong Li Zhe Yin Ke Zhang Xiaokai Dong State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology

To assess the risk of infectious bacterial aerosols leaking to the environment, the filtration efficiency of a biosafety level 3 （BSL-3） laboratory high-efficiency particulate （HEPA） filter was investigated using the aerosolized bacteria Serratia marcescens. The aerosol size was measured using an Andersen sampler. Eight first stage HEPA filters （numbered 1-87 were distributed in contaminated labs and exhausts from each of the first stage HEPA filters were aggregated and filtered through one second stage HEPA filter before being released to the environment. In total, 8 first-stage and I second-stage HEPA filters from the BSL-3 air purification system were analyzed. No S. marcescens was detected in first stage filters 1,2, 4, 5, 7 and 8 and the second stage HEPA filter. The filtration efficiencies against aerosolized S. marcescens were 〉99.9999%. First stage filter numbers 3 and 6 had filtration efficiencies of 99.9825% and 99.9906% respectively. When filter number 3 was replaced by a new filter and the bracket for filter number 6 was sealed, no aerosolized S. marcescens was detected in the filtered air. Our work suggests that the BSL-3 laboratory HEPA filter air purification system is effective against bacterial aerosols, with little to no bacterial leakage into the environment.

关键词： Bacterial aerosolFiltration efficiencyHEPA filtersSerratia marcescens

Evidence supporting the relationship between maternal asthma and risk for autism spectrum disorders

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Neural Regeneration Research 2025年第4期20卷 1101-1102页

作者： Hadley Osman Paul Ashwood Department of Medical microbiology and Immunology and the M.I.N.D.InstituteUniversity of California at DavisSacramentoCAUSA

During pregnancy,maternal immune activation(MIA),due to infection,chronic inflammatory disorders,or toxic exposures,can result in lasting health impacts on the developing *** has been associated with an increased risk of neurodevelopmental disorders,such as autism spectrum disorder(ASD)in the *** is characterized by increased repetitive and stereotyped behaviors and decreased *** of 2020,1 in 36 children are diagnosed with ASD by the age of 8 years,with ASD rates continuing to increase in prevalence in USA(Tamayo et al.,2023).Post-mortem brain studies,biomarker and transcriptomic studies,and epidemiology studies have provided compelling evidence of immune dysregulation in the circulation and brain of individuals diagnosed with ***,the etiology of ASD is largely unknown,however,genetic components and environmental factors can contribute to increased *** allergic asthma(MAA),a form of MIA,has been identified as a potential risk factor for developing neurodevelopmental disorders(Patel et al.,2020).Asthma is a chronic inflammatory condition driven by a T-helper type(TH)2 immune response.

关键词： disorders epidemiology fetus

Response of BALB/c mice to a monovalent influenza A （H1N1） 2009 split vaccine

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Cellular & Molecular Immunology 2010年第2期7卷 116-122页

作者： Penghui Yang Li Xing Chong Tang Weihong Jia Zhongpeng Zhao Kun Liu Xiao Gao Xiliang Wang beijing institute of microbiology and epidemiology State Key Laboratory of Pathogen and Biosecurity Beijing China

The novel influenza A （H1N1） 2009 virus has emerged to cause the first pandemic of the twenty-first century. Disease outbreaks caused by the influenza A （H 1N 1） virus have prompted concerns about the potential for a pandemic and have driven the development of vaccines against this subtype of influenza A. In this study, we developed a monovalent influenza A （H 1N 1） split vaccine and evaluated its effects in BALB/c mice. Mice were immunized subcutaneously with 2 doses of the vaccine containing hemagglutinin （HA） alone or HA plus an aluminum hydroxide （AI（OH）3） adjuvant. Immunization with varying doses of HA （3.75, 7.5, 15, 30, 45 or 60μg） was performed to induce the production of neutralizing antibodies. The vaccine elicited strong hemagglutination inhibition （HI） and microneutralization, and addition of the adjuvant augmented the antibody response. A preliminary safety evaluation showed that the vaccine was not toxic at large doses （0.5 ml containing 60 μg HA＋600 μg AI（OH）3 or 60 μg HA）. Moreover, the vaccine was found to be safe at a dose of 120 μg HA＋ 1200 μg AI（OH）3 or 120 μg HA in 1.0 ml in rats. In conclusion; the present study provides support for the clinical evaluation of influenza A （H1N1） vaccination as a public health intervention to mitigate a possible pandemic. Additionally, our findings support the further evaluation of the vaccine used in this study in primates or humans.

关键词： BALB/c mice influenza influenza A (H1N1) split vaccine

Over-expression of uPA increases risk of liver injury in pAAV-HBV transfected mice

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World Journal of Gastroenterology 2012年第16期18卷 1892-1902页

作者： Xiao-Jun Zhou Shi-Hui Sun Peng Wang Hong Yu Jing-Ya Hu Shi-Cheng Shang Yu-Sen Zhou beijing institute of microbiology and epidemiology Beijing 100071 China beijing Experimental Animal Center Beijing 100071 China

AIM:To investigate the relationship between overexpression of urokinase plasminogen activator(uPA) and hepatitis B virus(HBV) related liver diseases in a transgenic mouse ***:Albumin-tetracycline reverse transcriptional activator and tetO-uPA transgenic mice were generated respectively through pronuclear injection and crossed to produce the double transgenic in-alb-uPA mice,for which doxycycline(Dox)-inducible and liver-specific over-expression of uPA can be *** transfection of plasmid adeno-associated virus(AAV)1.3HBV was performed through the tail veins of the Dox-induced in-alb-uPA *** of uPA and HBV antigens were analyzed through double-staining immunohistochemical *** production was detected by enzyme linked immunosorbent assay and α-fetoprotein(AFP) mRNA level was evaluated through real-time quantitative polymerase chain ***:Plasmid AAV-1.3HBV hydrodynamic transfection in Dox-induced transgenic mice not only resulted in severe liver injury with hepatocarcinoma-like histological changes and hepatic AFP production,but also showed an increased serum level of HBV antigens and cytokines like interleukin-6 and tumor necrosis factor-α,compared with the control ***:Over-expression of uPA plays a synergistic role in the development of liver injury,inflammation and regeneration during acute HBV infection.

关键词： Tet-on system Albumin promoter Urokinase-type plasminogen activator Hydrodynamic transfection Liver injury

Formation and regulation of Yersinia biofilms

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Protein & Cell 2011年第3期2卷 173-179页

作者： Dongsheng Zhou Ruifu Yang State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

Flea-borne transmission is a recent evolutionary adaptation that distinguishes the deadly Yersinia pestis from its progenitor ***,a mild pathogen transmitted via the food-borne *** synthesizes biofilms in the flea gut,which is important for fleaborne *** biofilms are bacterial colonies surrounded by extracellular matrix primarily containing a homopolymer of N-acetyl-D-glucosamine that are synthesized by a set of specific *** biofilm production is tightly regulated at both transcriptional and post-transcriptional *** the known structural genes responsible for biofilm production are harbored in both *** and ***,but *** has evolved changes in the regulation of biofilm development,thereby acquiring efficient arthropod-borne transmission.

关键词： Yersinia pestis Y.pseudotuberculosis biofilm flea-borne transmission

Infectious Disease Surveillance in China

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Biomedical and Environmental Sciences 1998年第1期11卷 31-37页

作者： ZENG GUANG ZHANG JING-KUN ROU KE-MING, XU CHANG CHENG YING-KAI AND QI GUO-MING(The institute of epidemiology and microbiology, Chinese Academy ofPreventive Medicine, beijing 100050, China) institute of epidemiology and microbiology Chinese Academy of Preventive Medicine Beijing China.

A national system of infectious disease surveillance was established in 1959 in *** it consists of three subunits, namely, national disease reporting system (NDRS), nationwide disease surveillance points (DSPs), and surveillance network for specific infectious diseases. There are 35 notifiable infectious diseases, which are divided into Classes A, B, and C. The functions of the surveillance include explaining the natural history of infectious diseases, describing the distribution of case occurrence, triggering disease-control effort, monitoring epidemic of infectious diseases during natural disasters, predicting and controlling epidemics and providing the base of policy adjustment.

关键词： Infectious Disease Surveillance in China

EXPRESSION OF HBV PRE S1 PEPTIDE INE. Coli AND PRODUCT CHARACTERIZATION

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Chinese Medical Sciences Journal 1997年第1期12卷 37-40页

作者：米志宝郭巨涛冯福民陈文张习坦童贻刚 institute of microbiology and epidemiology Beijing 100071.

HBV Pre S1 sequence is supposed to play an important role in the infection of HBV. Presence of Pre S1 /anti-Pre S1 in serum has valuble clinical imphations. In order to improve the study of Pre S1, Pre S1 sequence was overexpressed in E. coli as a fusion protein with MBP (Maltose- binding Protein), and anti-Pre S1 antiserum was elicited in rabbits by Pre S1 MBP purified by affinity chromatography. The recombinant plasmid constructed from PMAL-cRI expressed the 106aa Pre S1 sequence at the C terminal of MBP by tac promoter. The resulting Protein is about 54kD in size. Western-blot analysis confirmed its reactivity with antiserum derived from synthetic Pre S1 peptide and serum from patients with acute hepatitis B (AHB). ELISA showed that Pre S1-MBP and Dane Particles purified from AHB patient’s serum reacted with antiserum against synthetic Pre S1 peptlde,and this reaction was specifically inhibited by synthetic Pre S1 peptide. ELISA also demonstrated that antiserum against Pre S1-MBP reacted with synthetic Pre S1 peptide, but not with synthetic HCV peptide or HEV peptide.

关键词： HBV gene expression E. coli

Immunological dynamics in response to two anthrax vaccines in mice

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Science China(Life Sciences) 2008年第10期51卷 872-878页

作者： Lü Jin, HE Rui, DONG Mei, ZHANG LiangYan & WANG XiLiang State Key Laboratory of Pathogen and Biosecurity, beijing institute of microbiology and epidemiology, beijing 100071, China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing China

In order to understand the variation of humoral and cellular immune responses to A16R live spore and AVA vaccine and to identify efficient immunological parameters for the early evaluation of post immu- nization in mice, we dynamically monitored the antibody production and cellular responses after the vaccination of Balb/C mice with the anthrax vaccines. The results show that both anti-AVA and anti-Spore antibodies were detectable in the A16R live spore vaccinated group while high titers of anti-AVA antibodies but not anti-Spore antibodies existed in the AVA-immunized group. IgG1 and IgG2 were the major subtypes of IgG in both of the two groups. However, the IgG2a level was significantly higher in the A16R group than in the AVA group. At the cellular level, responses of antigen-specific TH2, TH1 and plasma cells were detected. The peripheral TH2 responses could be seen on day 5 after vac- cination, and remained at a high level throughout the experiment (from day 5 post primary immuniza- tion to day 60 post the tertiary immunization); the TH1 responses to A16R vaccine appeared on day 5, while the responses to AVA could only be detected by day 7 after the secondary immunization; a low level of TH1 responses could be observed at the end of the experiment. Antigen-specific plasma cells could be found in the peripheral blood of both the immunized groups, however, the responses in the A16R group appeared earlier, lasted longer, and shown an ascending tendency until the end of the ex- periment when the plasma cell responses in the AVA group were reduced to a very low level. The re- sults suggest that the multiple antigen containing A16R live spore vaccine induces better immune re- sponses than AVA. Combined with serum antibody titers, TH2, TH1 and plasma cell responses could be used as immunological parameters for the evaluation of vaccine efficacy. These findings may afford new insight into the early evaluation of vaccination as well as being a powerful strategy for vacci

关键词： anthrax vaccine adsorbed live spore vaccine antigen secreting cells immunological response ELISPOT