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A flow cytometer based protoco. for quantitative analysis of bloom-forming cyanobacteria(Microcystis) in lake sediments

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Journal of Environmental Sciences 2012年第9期24卷 1709-1716页

作者： Quan Zhou Wei Chen Huiyong Zhang Liang Peng Liming Liu Zhiguo Han Neng Wan Lin Li Lirong Song State Key Laboratory of Freshwater Eco.ogy and biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of Chinese Academy of Sciences Beijing 100039 China Zealquest Laboratory for Eco.ogical Research Zealquest Scientific Technology Co. Ltd. Shanghai 200333 China Changshu Institute of Technology Changshu 215500 China

A quantitative protoco. for the rapid analysis of Microcystis cells and co.onies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples co.taining Microcystis were processed with sonication to disintegrate co.onies into single cells. An optimized procedure suggested that 5 mg dw （dry weight）/mL dilution co.bined with 200 W x 2 min sonication yielded the highest co.nting efficiency. Under the optimized determination co.ditions, the quantification limit of this protoco. was 3.3x104 cells/g dw. For co.ony analysis, Microcystis were isolated from the sediment by filtration. co.ony lengths measured by flow cytometry were similar to those measured by microsco.y for the size range of one single cell to almost 400 ~tm in length. Moreover, the relationship between co.ony size and cell number was determined for three Microcystis species, including Microcystisflos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different- sized co.onies. The developed protoco. was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium.

关键词： flow cytometry benthic Microcystis cell enumeration co.ony size measurement

Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect co.petitive ELISA to detect diethylstilbestrol in biological samples

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Chinese Science Bulletin 2011年第8期56卷 749-754页

作者： LI WeiHua MENG Meng HE FangYang WAN YuPing XUE HuYin LIU Wei YIN WeiWei XU Jing FENG CaiWei WANG ShanLiang LU Xiao LIU JinTing XI RiMo School of Chemistry and Chemical Engineering Shandong University Jinan 250100 China co.lege of Pharmacy and Tianjin key Laboratory of Molecular Drug Research Nankai University Tianjin 300071 China beijing Wanger biotechnology co. Ltd. Beijing 102206 China Central Laboratory of Shandong Academy of Agricultural Sciences Jinan 250100 China

Based on the preparation of an anti-diethylstilbestrol(DES) monoclonal antibody,a simple and co.venient indirect co.petitive enzyme-linked immunosorbent assay(ELISA) method for DES detection has been *** monoclonal antibody demonstrated high sensitivity to DES with an IC50 value of 275 pg mL-1 and detection limit(LOD) of 90 pg *** specificity of the assay was studied by measuring cross-reactivity of the antibody with structurally related co.pounds of ethinyl estradiol(co.centration of DES were detected by the developed method,and the reco.ery rates were greater than 79.5%.Intra-and inter-assay variations were about 6%.This method exhibited high stability with a co.fficient of variation less than 10% in buffer and in real *** LODs in fish/shrimp,liver,feed and urine spiked with DES were 600,600,4800 and 600 pg mL-1,*** results co.firmed that the antibody to DES was successfully produced and co.ld be used to establish ELISA methods for DES detection in food producing animals.

关键词： ELISA检测方法单克隆抗体己烯雌酚生物样品竞争间接酶联免疫吸附试验 DES

Multicenter evaluation of a simple and sensitive nucleic acid self-testing for SARS-co.-2

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Virologica Sinica 2023年第4期38卷 620-626页

作者： Xin-Xin Shen Feng-Yu Li Meng Qin Guo-Hao Zhang Meng-Yi Zhang Hong Liu Xiu-Li Sun Zhen-Jiang Xin Xue-Jun Ma NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing102206China Hebei Medical University Shijiazhuang050031China Fengtai District Center for Disease co.trol and Prevention of beijing Beijing100071China beijing Baicare biotechnology co. Ltd.Beijing102206China Shandong University of Technology School of Life Sciences and MedicineZibo255000China

A rapid and accurate co.ID-19 diagnosis is a prerequisite for blocking the source of infection as soon as possible and taking the appropriate medical ***,we developed GeneClick,a device for nucleic acid self-testing of SARS-co.-2,co.sisting of three modules:a sampling kit,a microfluidic chip-based disposable cartridge,and an amplification *** addition,we evaluated the clinical performance of GeneClick using 2162 nasal swabs co.lected at three medical institutions,using three co.mercial RT-qPCR kits and an antigen self-test as *** to RT-qPCR,the sensitivity and specificity of the GeneClick assay were 97.93%and 99.72%,respectively,with a kappa value of 0.979(Pco.-2 *** the 314 positive samples identified by GeneClick assay,63 samples were undetected by antigen ***,the GeneClick nucleic acid self-test demonstrated higher accuracy than the antigen-based *** on the additional features,including simple operation,affordable price,portable device,and reliability of smartphone APP-driven sampling and result reporting,GeneClick offers a powerful tool for field-based SARS-co.-2 detection in primary healthcare institutions or at-home use.

关键词： Nucleic acid self-testing SARS-co.-2 RT-qPCR Antigen-test

Study on the Efficacy of A Whitening Product

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China Detergent & co.metics 2020年第3期5卷 68-72页

作者： Wang Peng Zhang Yunxian Du Yulan Zhang Pengxiang Wu Jinhao Tianjin Kangting biotechnology co. Ltd.China beijing Yunzhiqingshui Technology co. LtdChina beijing Yiweishi Testing Technology co.ltd. China

A whitening essence lotion was prepared and its whitening and moisturizing efficacy was studied.24 female volunteers were selected to use the whitening essence lotion on the inner side of their forearm as required for 8 weeks *** changes of MI value,L*value,ITA°value and the moisture co.tent of skin stratum co.neum were tested for the skin at co.responding *** results showed that,after using co.metics for eight weeks,the skin’s MI value,L^* value,ITA°value and moisture co.tent were significance level from the initial values(P<0.05).The product showed certain effects of whitening and moisturizing.

关键词： co.metic efficacy whitening moisturizing

Letter to the editor: Neutralizing antibodies for the treatment of co.ID-19

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Acta Pharmaceutica Sinica B 2021年第1期11卷 304-307页

作者： Zeqi Zhou Xiangbin Wang Yankai Fu Xiqing Zhang Changxiao Liu Dynamiker biotechnology Sub-Center Beijing Key Laboratory for Mechanism Study and Precision Diagnosis of Invasive Fungal DiseasesTianjin 300467China beijing Antai Zhiyuan Technology co. Ltd.Beijing 102206China State Key Laboratory of Drug Delivery Technology and Pharmaco.inetics Tianjin Institute of Pharmaceutical ResearchTianjin 300462China

To the Editor:co.onavirus disease 2019(co.ID-19)is a global epidemic caused by the infection of severe acute respiratory co.onavirus 2(SARS-co.V-2)and its mortality rate was up to 20 times higher than that of *** viru... 详细信息

关键词： SARS-co.-2 Neutralizing antibody therapy co.ID-19

A Double-Phase High-Frequency Traveling Magnetic Field Developed for co.tactless Stirring of Low-co.ducting Liquid Materials

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co.puter Modeling in Engineering & Sciences 2021年第5期127卷 469-486页

作者： Xiaodong Wang Ernst Roland Fautrelle Yves co.lege of Material Science and Opto-Electronic Technology University of the Chinese Academy of ScienceBeijingChina Guangwei(Zhongshan)Intelligent Technology co. Ltd.Huairou Science CityBeijingChina SIMAP/EPM Universite Grenoble AlpesGrenobleFrance

The use of low electrically co.ducting liquids is more and more *** is the case for molten glass,salt or slag processing,ionic liquids used in biotechnology,batteries in energy storage and *** present paper deals with the design of a new electromagnetic induction device that can heat and stir low electricallyco.ducting *** co.sists of a resistance-capacity-inductance circuit co.pled with a low-co.ducting liquid *** device is supplied by a unique electric power source delivering a single-phase high frequency electric *** main working principle of the circuit is based on a double oscillating circuit inductor co.nected to the solid-state transistor *** technique,which yields a set of co.pled oscillating circuits,co.sists of co.pling a forced phase and an induced phase,neglecting the influence of the electric parameters of the loading part(i.e.,the low-co.ductivity liquid).It is shown that such an inductor is capable to provide a two-phase AC traveling magnetic field at high *** better understand the working principle,the present work improves a previous existing simplified theory by taking into acco.nt a co.plex electrical equivalent diagram due to the different mutual co.plings between the two inductors and the two co.responding induced current sets.A more detailed theoretical model is provided,and the key and sensitive elements are *** on this theory,equipment is designed to provide a stirring effect on sodium chloride-salted water at 40 S/*** is shown that such a device fed by several hundred kiloHertz electric currents is able to mimic a linear motor.A set of optimized operating parameters are proposed to guide the experiment.A pure electromagnetic numerical model is *** modelling of the load is performed in order to assess the efficiency of the stirrer with a salt water *** a device can generate a significant liquid motion with both co.trolled flow patterns and adjus

关键词： Alternating magnetic field electromagnetic stirring low electrically co.ducting liquids ionic liquid

A Highly Attenuated Mumps Virus Strain of Genotype F Generated by Passaging in Vero Cells

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Virologica Sinica 2021年第2期36卷 337-340页

作者： Yajing Zhang Lixia Xie Benjie Chai Juncheng Ruan Yulin Gu Biao Niu Yachun Zhang Zhenfang Fu Qi An Dayong Tian R&D Department Shanghai King-cell biotechnology Co.Ltd.Shanghai 201506China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070China

Dear Editor Mumps,caused by mumps virus(MuV),is still a serious threat to the health of children and *** most mumps cases,the clinical symptoms were parotitis,myalgia,malaise,fever and *** a neurotropic virus,MuV infection also causes cerebrospinal fluid pleocytosis in one-third of patients and meningitis in 10%of *** very few cases,orchitis and oophoritis also can occur(Carbone and Wolinsky 2001).

关键词： cases clinical patients

Molecular Weight co.trollable Degradation of Laminaria japonica Polysaccharides and Its Antioxidant Properties

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Journal of Ocean University of China 2016年第4期15卷 637-642页

作者： ZHA Shenghua ZHAO Qingsheng ZHAO Bing OUYANG Jie MO Jianling CHEN Jinjin CAO Lili ZHANG Hong Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 P. R. China Graduate University of Chinese Academy of Sciences Beijing 100049 P. R. China co.lege of Biological Science and biotechnology Beijing Forestry University Beijing 100083 P. R. China beijing Tong Ren Tang Health Pharmaceutical co. Ltd. Beijing 100085 P. R. China

In this study, molecular weight co.trollable degradation of algal Laminaria japonica polysaccharides(LPS) was investigated by ultrasound co.bined with hydrogen peroxide. Three main factors, i.e., ultrasonic power(A), ultrasonic time(B), and H_2O_2 co.centration(C) were chosen for optimizing parameters by employing three-factors, three-levels BBD. The influence of degradation on structure change and antioxidant activities was also investigated. A seco.d-order polynomial equation including molecular weight(Y) of Laminaria japonica polysaccharides and each variable parameter, i.e., ultrasonic power(A), ultrasonic time(B), and H_2O_2 co.centration(C), was established: Y=20718.67-4273.13A-4000.38B-1438.75C+2333.25AB+1511.00AC+873.00BC+2838.29A^2 + 2490.79B^2+873.04C^2. The equation regression co.fficient value(R^2 = 0.969) indicated that this equation was valid. The value of the adjusted determination co.fficient(adjusted R^2 = 0.914) also co.firmed that the model was highly significant. The results of selected experimental degradation co.ditions matched with the predicted value. FT-IR spectra revealed that the structures of LPS before and after degradation were not significantly changed. Antioxidant activities of LPS revealed that low Mws possessed stronger inhibitory than the original polysaccharides. The scavenging effects on superoxide radicals was the highest when IC50 of crude LPS was 4.92 mg mL^(-1) and IC50 of Mw 18.576 KDa was 1.02 mg mL^(-1), which was fourfold higher than initial polysaccharide.

关键词： Laminaria superoxide scavenging ultrasonic co.trollable adjusted optimizing matched degraded visco.ity

A New Method for Blood NT-proBNP Determination Based on a Near-infrared Point of Care Testing Device with High Sensitivity and Wide Sco.e

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Biomedical and Environmental Sciences 2017年第6期30卷 426-431页

作者： ZHANG Xiao Guang SHU Yao Gen GAO Ju WANG Xuan LIU Li Peng WANG Meng CAO Yu Xi ZENG Yi National lnstitute for Viral Disease co.trol and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China beijing Runbio biotechnology Development co. Ltd Beijing 100106 China No.2 People's Hospital of Dali City Dali 671003 Yunnan China Department of Laboratory Medicine Beijing Hospital National Center of Gerontology Beijing 100730 China

Objective To develop a rapid, highly sensitive, and quantitative method for the detection of NT-proBNP levels based on a near-infrared point-of-care diagnostic （POCT） device with wide sco.e. Methods The lateral flow assay （LFA） strip of NT-proBNP was first prepared to achieve rapid detection. Then, the antibody pairs for NT-proBNP were screened and labeled with the near-infrared fluorescent dye Dylight-800. The capture antibody was fixed on a nitrocellulose membrane by a scribing device. Serial dilutions of serum samples were prepared using NT-proBNP-free serum series. The prepared test strips, co.bined with a near-infrared POCT device, were validated by known co.centrations of clinical samples. The POCT device gave the output of the ratio of the intensity of the fluorescence signal of the detection line to that of the quality co.trol line. The relationship between the ratio value and the co.centration of the specimen was plotted as a work curve. The results of 62 clinical specimens obtained from our method were co.pared in parallel with those obtained from the Roche E411 kit. Results Based on the log-log plot, the new method demonstrated that there was a good linear relationship between the ratio value and NT-proBNP co.centrations ranging from 20 pg/mL to 10 ng/mL. The results of the 62 clinical specimens measured by our method showed a good linear co.relation with those measured by the Roche E411 kit. co.clusion The new LFA detection method of NT-proBNP levels based on the near-infrared POCT device was rapid and highly sensitive with wide sco.e and was thus suitable for rapid and early clinical diagnosis of cardiac impairment.

关键词： NT-proBNP Near-infrared POCT device Lateral flow assay