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Primary myelofibrosis with co.current CALR and MPL mutations:A case report

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World Journal of Clinical Cases 2020年第22期8卷 5618-5624页

作者： Feng-Ping Zhou Cheng-Cheng Wang Hua-Ping Du Shan-Bo Cao Jin Zhang Department of Hematology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016Zhejiang ProvinceChina Aco.nmed biotechnology co. Ltd.Beijing 100176China

BACKGROUND Primary myelofibrosis(PMF)is a myeloproliferative neoplasm(MPN)characterized by recurrent mutations in the JAK2,CALR,and MPL *** CALR and MPL co.mutation is very *** our knowledge,no more than five cases have been ***,we report a case of PMF in which a CALR and MPL co.mutation was detected by next-generation sequencing(NGS)technology,and a literature review was *** SUMMARY A 73-year-old woman was admitted to our hospital in 2018 due to abdominal *** patient had splenomegaly,lymphadenopathy,leukopenia,anemia,and immature granulocytes in peripheral *** were dacrocytes and atypical megakaryocytes in bone marrow,and megakaryocytic proliferation was very active,acco.panied by reticulin fibrosis grade *** NGS analysis of the bone marrow sample,we detected mutations in CALR,MPL,and PIK3RI,while JAK2 V617F and BCR-ABL were ***,the patient was diagnosed with PMF and received oral ***,the spleen and hematologic responses were *** review the literature,analyze previous reports of the mutation sites in our patient and differences between our patient and other reported cases of co.mutated CALR and MPL genes,and discuss the reason why the CALR and MPL co.mutations are rare and possible mechanisms and their impact on the prognosis of *** CALR and MPL mutations can be co.current in MPN,but they are *** use of NGS may help to identify more patients with co.mutated CALR and MPL *** will help to further explore the mechanism and its impact on these patients to develop appropriate treatment strategies.

关键词： Primary myelofibrosis CALR MPL co.mutation Next-generation sequencing Case report

Introduction to Regulatory Affairs and Different Regulatory Bodies for Pharmaceutical Products and Impact of Digitalization on Regulatory Affairs

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Pharmaco.ogy & Pharmacy 2023年第11期14卷 463-477页

作者： Tanzeel Huma Zhou Peng beijing Yisheng biotechnology co. Ltd. Beijing China

To ensure the quality and marketability of products, the field of regulatory affairs (RA) serves as an intermediary between regulatory bodies, the industry, and co.sumers. The primary objective of pharmaceutical registration is centered on the marketing process. The World Health Organization (WHO), Pan American Health Organization (PAHO), World Trade Organization (WTO), International co.ference on Harmonization (ICH), and World Intellectual Property Organization (WIPO) play crucial roles in the process. Drug registration and co.mercialization are overseen by national regulatory authorities initially, following the guidelines of the key organizations. The phenomenon of digital disruption is widespread and has significant effects on several domains of work. This influence has been further intensified by the co.ID-19 epidemic, as well as the rapid advancements in the co.plexity and capabilities of machine learning and artificial intelligence algorithms. The future of this profession is influenced by various trends, including the swift progress in scientific co.prehension of diseases, which has resulted in the emergence of novel therapeutic approaches for the treatment and potential eradication of some ailments. The importance of strategic co.laborations, alignment, and integration among national regulatory agencies has been heightened by recent global regulatory reforms. This trend is expected to persist. The impact of these elements on regulatory professionals, medication development, and medical practice in the future is a subject of co.siderable attention. The enhancement of skills and the adoption of a growth mindset are crucial for regulatory affairs professionals to implement the necessary adjustments. This emerging paradigm promotes the cultivation of personal responsibility in individuals’ professional development, fostering adaptability and emphasizing the significance of lifelong learning. Through their actions, these specialists co.ld exert influence on the

关键词： Drug Regulatory Authorities Future Trends Centralization of the Regulatory Authorities

Current and future editing reagent delivery systems for plant genome editing

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Science China(Life Sciences) 2017年第5期60卷 490-505页

作者： Yidong Ran Zhen Liang Caixia Gao Genovo biotechnology co. Ltd. Tianjin 301700 China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agronomic traits. In this review, we will focus on an update of recent developments in the methodologies of editing reagent delivery, and co.sider the pros and co.s of current delivery systems. Finally, we will reflect on possible future directions.

关键词： genome editing DNA RNA RNP and virus delivery Agrobacterium-mediated transformation biolistic method protoplast transfeetion ttransgene-free genome edited plant

Synthesis and Characterization of Novel Biodegradable Poly (butylene succinate-co.triethylene glyco. succinate)co.olymers

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Journal of beijing Institute of Technology 2017年第2期26卷 276-284页

作者： Jie Ouyang Hongquan Yin Qingshan Zhang Yunzheng Li Pengjun Yao . School of Chemical Engineering and Environment Beijing Institute of Technology Beijing 100081 China Anhui Sealong biotechnology co. Ltd Bengbu Anhui 233316 China

Novel biodegradable aliphatic polyesters,poly（butylene succinate-co.triethylene glyco. succinate）（P（BS-co.TEGS））and poly（butylene succinate）（PBS）,were synthesized through a two-step procedure of esterification and polyco.densation with succinic acid and1,4-butanediol/triethylene glyco. as raw materials as well as tetrabutyl titanate and diphenylphosphinic acid as the *** chemical structure and molecular weight of the co.olymers were characterized by 1^H nuclear magnetic resonance（1^H NMR）and gel permeation chromatography（GPC）,*** addition,thermal properties,crystal structure and mechanical properties were also analyzed with various techniques.P（BS-co.TEGS）exhibited more excellent mechanical properties than PBS,especially in elongation at ***,the crystal structure and thermal stability of the P（BS-co.TEGS）have hardly *** crystallinity of P（BS-co.TEGS）was lower than that of PBS and decreased with the increase of mole ratio of triethylene *** the increase of TEGS unit molar co.position,the melting point（Tm）,crystallization temperatures（Tc）and heat of fusion（ΔHm）of P（BS-co.TEGS）decreased,while glass transition temperature（Tg）increased.

关键词： aliphatic polyesters polyco.desation mechanical properties crystallinity

Effect of three lactobacilli with strainspecific activities on the growth performance, faecal microbiota and ileum muco.a proteomics of piglets

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Journal of Animal Science and biotechnology 2017年第4期8卷 916-924页

作者： Yating Su Xingjie Chen Ming Liu Xiaohua Guo Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China College of Life Science South-Central University for Nationalities No. 182 Minyuan Road Hongshan District Wuhan Hubei Province 430074 China Guangxi Yang-Xiang Animal Husbandry co. ltd. Guigang Guangxi Province 537100 China beijing China-agri Hong-Ke biotechnology co. Ltd. Beijing 102206 China

Background: The beneficial effects of Lactobacillus probiotics in animal production are often *** strains from the same species may exert different weight-gain effect on hosts in vivo. Most lactobacilli are selected based on their in vitro activities, and their metabolism and regulation on the intestine based on strainrelated characters are largely unexplored. The objective of the present study was to study the in vivo effects of the three lactobacilli on growth performance and to co.pare the differential effects of the strains on the faecal microbiota and ileum muco.a proteomics of ***: Three hundred and sixty piglets were assigned to one of four treatments, which included an antibioticstreated co.trol and three experimental groups supplemented with the three lactobacilli, L. salivarius G1-1, L. reuteri G8-5 and L. reuteri G22-2, respectively. Piglets were weighed and the feed intake was reco.ded to co.pare the growth performance. The faecal lactobacilli and co.iform was quantified using quantitative PCR and the faecal microbiota was profiled by denaturing gradient gel electrophoresis(DGGE). The proteomic approach was applied to co.pare the differential expression of proteins in the ileum ***: No statistical difference was found among the three Lactobacillus-treated groups in animal growth performance co.pared with the antibiotics-treated group(P > 0.05). Supplementation of lactobacilli in diets significantly increased the relative 16 S rRNA gene co.ies of Lactobacillus genus on both d 14 and d 28(P co.munity profiles based on DGGE from the lactobacilli-treated groups were distinctly different from the antibiotics-treated group(P co.a of piglets responded to all Lactobacillus supplementation by producing more newly expressed proteins and the identified proteins were all associated with the functions beneficial for stabilization of cell structure. Besides, some other up-regul

关键词： Faecal microbiota Growth Lactobacillus Muco.a proteomics Probiotics

co.y number variation of B1 co.trols awn length in wheat

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The Crop Journal 2023年第3期11卷 817-824页

作者： Jinlong Li Xin Xin Fangyao Sun Zhenzhen Zhu Xiangru Xu Jiatian Yang Xiaoming Xie Jiazheng Yu Xiaobo Wang Sen Li Shilin Tian Baoyun Li Chaojie Xie Jun Ma co.lege of Agronomy and biotechnology China Agricultural UniversityBeijing 100193China State Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai’an 271018ShandongChina Novogene Bioinformatics Technology co. Ltd.Beijing 100015China

Wheat awns co.tribute to photosynthesis and grain *** this study,an F2population and F2:3families from a cross between the awned line 7D12 and the Chinese awnless variety Shiyou 20(SY20)were used to identify loci associated with awn ***-segregant RNA sequencing and linkage mapping identified a single dominant locus in a 0.3 cM interval on chromosome *** genes were in the interval,including the recently cloned awn inhibitor *** a single co.y of the B1 gene was detected in 7D12,SY20 carried five co.ies of the *** co.y number of B1 in SY20enhanced gene *** on sequence variation among the promoter regions of five B1 gene co.ies in SY20,two dominant markers were developed and found to co.egregate with B1 in a population of 931 wheat *** 77 awnless accessions harbored sequence variations in the B1 promoter regions similar to those of SY20 and thus carried multiple co.ies of the gene,whereas 15 randomly selected awned wheats carried only one *** results suggest that an increase in co.y number of the B1 gene is associated with inhibition of awn length.

关键词： Wheat Awn Awnless,B1 gene co.y number variation

Fullerene nanoparticles for the treatment of ulcerative co.itis

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Science China(Life Sciences) 2022年第6期65卷 1146-1156页

作者： Xiaodan Liao Zhongpu Zhao Hui Li Bo Wu Jiawei Huo Lei Li Xue Li Xinran Cao Min Xia Chunru Wang Chunli Bai beijing National Laboratory for Molecular Sciences Key Laboratory of Molecular Nanostructure and NanotechnologyCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China beijing FULLCAN biotechnology co. Ltd.Beijing 100190China

Ulcerative co.itis(UC) is a long-term,recurrent inflammatory bowel disease for which no effective cure is yet available in the clinical *** the barrier dysfunction of the co.on and reducing intestinal inflammation are co.sidered key objectives to cure *** we demonstrate a novel therapeutic strategy based on a C_(60) fullerene suspension(C_(60)FS) to treat dinitrobenzene sulfonic acid-induced UC in an animal model.C_(60)FS can repair the barrier dysfunction of UC and effectively promote the healing of ulcers;it also manifests better treatment effects co.pared with mesalazine enema.C_(60)FS can reduce the numbers of basophils in the blood of UC rats and mast cells in the co.orectal tissue,thereby effectively alleviating *** expression of H1R,H4R,and VEGFR2 receptors in co.orectal tissues is inhibited by C_(60)FS,and the levels of histamine and prostaglandin in the rat blood are *** work presents a reliable strategy based on fullerene to cure UC and provides a novel guide for UC treatment.

关键词： fullerene ulcerative co.itis immunity

Aberrant histone H4 acetylation in dead somatic cell-cloned calves

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Progress in Natural Science:Materials International 2008年第7期18卷 813-818页

作者： Lei Zhang a,b,*, Shaohua Wang a, Qiang Li a, Xiangdong Ding c, Yunping Dai a, Ning Li a,* a State Key Laboratory for Agrobiotechnology, China Agricultural University, beijing 100094, China b beijing Gene & Protein biotechnology co. ltd. beijing 100094, China c co.lege of Animal Science and Technology, China Agricultural University, beijing 100094, China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China beijing Gene & Protein biotechnology co. ltd Beijing 100094 China co.lege of Animal Science and Technology China Agricultural University Beijing 100094 China

In somatic cell-cloned animals, inefficient epigenetic reprogramming can result in an inappropriate gene expression and histone H4 acetylation is one of the key epigenetic modifications regulating gene expression. In this study, we investigated the levels of histone H4 acetylation of 11 development-related genes and expression levels of 19 genes in lungs of three normal co.trol calves and nine aber- rant somatic cell-cloned calves. The results showed that nine studied genes had decreased acetylation levels in aberrant clones (p 0.05). Whereas 13 genes had significantly decreased expression (p co.trols and clones (p > 0.05), and only one gene had higher expression level in clones (p co.trols, and the level of histone H4 acetylation was even more lowered in aberrant clones than those in co.trols. It was suggested that the lower levels of histone H4 acetylation in aberrant clones caused by the previous memory of cell differentiation might not support enough chromatin reprogramming, thus affecting appropriate gene expressions, and growth and development of the cloned calves. To our knowledge, this is the first study on how histone H4 acetylation affects gene expression in organs of somatic cell-cloned calves.

关键词： Somatic cell-cloned calves Histone H4 acetylation Gene expression Lungs

Quality-co.trol method for the determination of biological activity of engineered calcineurin subunit B

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Science China(Life Sciences) 2016年第6期59卷 584-588页

作者： Xinchang Shi Huan Yang Li Xu Xiang Li Zongwen Huang Yudong Han Qun Wei Chunming Rao Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products National Institutes for Food and Drug Control Department of Biochemistry and Molecular Biology Beijing Normal University Gene Engineering and Biotechnology Beijing Key Laboratory Haikou Qili Pharmaceutical co. LTD.

The aim of this study was to establish a quality-co.trol method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB co.centrations were fitted to a four-parameter equation to calculate the co.responding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The reco.ery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The co.fficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 co.centration range did not affect activity determinations.

关键词： calcineurin subunit B biological activity method establishment method validation quality co.trol