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Copper-catalyzed C-H bond functionalization enabled by programmed alternating current

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Science China Chemistry 2024年

作者： Zhoumei Tan Kun Xu Chengchu Zeng beijing key laboratory of environmental and viral oncology College of Chemistry and Life Science Beijing University of Technology

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Electrocatalytic Synthesis of Non-Symmetric Biphenols Mediated by Tri(p-bromophenyl)amine:Selective Oxidative Cross-Coupling of Different Phenols and Naphthols

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Chinese Journal of Chemistry 2019年第4期37卷 352-358页

作者： Qing-Qing Wang Yang-Ye Jiang Cheng-Chu Zeng Bao-Guo Sun beijing Advanced Innovation Center for Food Nutrition and Human Health School of Food and Chemical EngineeringBeijing Technology and Business UniversityBeijing 100048China beijing key laboratory of environmental and viral oncology College of Life Science & BioengineeringBeijing University of TechnologyBeijing 100124China

An efficient electrochemical access to the non-symmetric biphenols using tri(p-bromophenyl)amine(TBPA)as a redox mediator has been *** electrochemical protocol features highly selective cross-coupling products in up t... 详细信息

关键词： Non-Symmetric Biphenols Mediated Tri(p-bromophenyl)amine Phenols Naphthols

A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus

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Biomedical and environmental Sciences 2019年第5期32卷 357-362页

作者： WANG Qian Ying LI Fan SHEN Xin Xin FU Shi Hong HE Ying LEI Wen Wen LIANG Guo Dong WANG Huan Yun MA Xue Jun Department of viral Encephalitis NHC Key Laboratory of BiosafetyNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China State key laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionBeijing 102206China NHC key laboratory of Medical Virology and viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.

关键词： Tick‐borne encephalitis virus Subtype Far‐eastern Detection RT‐RAA

Plant flowers transmit various bio-agents through air

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Science China Earth Sciences 2020年第10期63卷 1613-1621页

作者： Siyu XU Maosheng YAO State key Joint laboratory of environmental Simulation and Pollution Control College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China

Growing air pollution,known as"April snow",by fluffy catkins from plant flowering was frequently observed during the Spring time in *** efforts mainly focus on controlling catkin release,while their public health and environmental impacts are ***,using culturing,microscopic and sequence methods we found that the flying catkins carry a significant amount of bio-agents(bacteria,fungal,viruses,and pollens),e.g.,up to 6.33×10^4 bacterial and 7.46×10^5 fungal cells per *** pollens from plants were also found riding on the flying *** collected fluffy catkins,bacterial Paracoccus,Massilia,fungal Ophiocordyceps,Arthrocladiella,pollen Robina,Pickeringia,Pinus,and viral family Genomoviridae,to name a few,were found to be *** infection and allergic potentials,the detected microbial structures on the fluffy catkins were different from those of local air,varying from one location to *** not just white pollution,flying plant fluffy catkins were discovered here also acting as a cloud of biological agents,and further actions need to be taken urgently to understand and limit their environmental and ecological consequences.

关键词： Plant flowering Flying fluffy catkins Bio-agents Transmission Air pollution

Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1111-1122页

作者： Jie Chen Yan Wang Weimin Zhang Di Zhao Lingyuan Zhang Jiawen Fan Jinting Li Qimin Zhan key laboratory of Carcinogenesis and Translational Research(Ministry of Education/beijing) Laboratory of Molecular OncologyPeking University Cancer Hospital&Institute100142 BeijingChina

Reactive oxygen species(ROS)localized at the precise subcellular compartments are essential for regulating the activity of signaling ***,ROS are master regulators of tumor malignant progression that respond to a diverse set of environmental stress,especially *** oxidases(NOXs)appear to be activated within discrete subcellular compartments to facilitate local ROS ***,the subcellular function of NOXs in hypoxic tumor is still *** this study,we demonstrated that NOX5 was greatly upregulated in clinical esophageal squamous cell carcinoma(ESCC)tumors,ESCC cell lines or primary ESCC cells,and elevated NOX5 was correlated to malignancy of ESCC tumors and poor ***5 induced the malignant progression of ESCC by activating Src,especially under hypoxic ***,we showed that hypoxia promoted the interaction between NOX5 and Pyk2 on cell membrane via facilitating Ca^(2+)-mediated Pyk2 Tyr^(402)site ***,Pyk2 acted as a scaffold for c-Abl phosphorylating the catalytic domain of NOX5 Tyr^(476/478)sites,which in turn upregulated hydrogen peroxide(H_(2)O_(2))inside the Pyk2/NOX5 complex to oxidize and activate local *** findings provide insights into the biological significance of NOX5 in the development of ESCC.

关键词： malignancy elevated esophageal

Prevalence of human papillomavirus in esophageal carcinoma in Tangshan,China

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World Journal of Gastroenterology 2015年第10期21卷 2905-2911页

作者： Mohammadreza Mohammadzad Mehryar Shu-Ying Li Hong-Wei Liu Fan Li Fang Zhang Yu-bai Zhou Yi Zeng Jin-tao Li beijing key laboratory of environmental and viral oncology College of Life Science and Bio-EngineeringBeijing University of Technology Collaborative Innovation Center for Biotherapy West China HospitalSichuan University Department of Pathogenic Biology College of Basic Medicine Hebei United University key laboratory of Southwest China Wildlife Resources Conservation Ministry of EducationCollege of Life Sciences China West Normal University National Institute for viral Disease Control and Prevention Chinese Center for Disease Control and Prevention and State Key Laboratory for Infectious Disease Prevention and Control

AIM:to study the prevalence of human papillomavirus(HPV) in esophageal carcinoma in tangshan,China,a high-incidence ***:Formalin-fixed,paraffin-embedded tissue specimens from 198 patients who were pathologically diagnosed with esophageal squamous cell carcinoma from 2011 to 2013 were obtained from a pathology department in *** was extracted from all198 specimens to detect HPV by polymerase chain reaction(PCR).β-globin PCR was performed to check the quality of the DNA extraction *** was performed to detect a wide range of HPV types,and type-specific PCR was performed to detect HPV types16 and *** and positive controls were used for HPV 16 and 18 ***:the DNA extraction method in this study appeared to be more effective than other previously reported *** DNA extraction,more than98%of the tissue specimens had an acceptable result in the DNA qualification test(β-globin PCR).the overall prevalence of HPV in tumor tissues by GP6+/GP5+PCR was 79.79%,and the prevalence of HPV types16 and 18 was 40.40%and 47.47%,*** demonstrated the presence of HPV,and direct sequencing confirmed the HPV *** HPVpositive PCR products were checked by DNA sequence analysis using DNAman and compared with the known HPV sequences listed in the basic Local Alignment Search tool database to evaluate the HPV *** analysis confirmed the presence of HPV types 16 ***:DNA of high-risk HPV types 16 and 18is present in esophageal tumors,implicating HPV as a possible etiologic factor for esophageal squamous cell carcinoma.

关键词： Esophageal carcinoma Formalin-fixed,paraffin-embed

TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus

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Biomedical and environmental Sciences 2018年第3期31卷 208-214页

作者： SHAO Nan LI Fan NIE Kai FU Shi Hong ZHANG Wei Jia HE Ying LEI Wen Wen WANG Qian Ying LIANG Guo Dong CAO Yu Xi WANG Huan Yu Department of viral Encephalitis National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China State key laboratory of Infectious Disease Prevention and Control Beijing 102206 China State key laboratory for genetic engineering and molecular virology National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206China School of Public Health Shandong University Jinan 250012 Shandong China Office of laboratory management National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To detect Japanese encephalitis virus（JEV） rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction（RT-PCR） detection system was *** By aligning the full-length sequences of JEV（G1-G5）, six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR *** With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/μL. The coefficients of variation of this real-time RT-PCR were all 〈 2.8%. The amplification efficiency of this method was between 90% and 103%.Conclusion A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes.

关键词： Japanese encephalitis virus Genotype TaqMan real-time RT-PCR

Occurrence and Decay of SARS-CoV-2 in Community Sewage Drainage Systems

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Engineering 2023年第7期26卷 214-219,I0008,I0009页

作者： Qian Dong 蔡俊雄刘艳臣凌海波 Qi Wang Luo-Jing Xiang Shao-Lin Yang Zheng-Sheng Lu Yi Liu Xia Huang 曲久辉 State key Joint laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China Hubei Provincial Academy of Eco-environmental Sciences Wuhan 430072China key laboratory of Drinking Water Science and Technology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

The rapid spread of the coronavirus disease(COVID-19)pandemic in over 200 countries poses a substantial threat to human *** acute respiratory syndrome coronavirus 2(SARS-CoV-2),which causes COVID-19,can be discharged with feces into the drainage ***,a comprehensive understanding of the occurrence,presence,and potential transmission of SARS-CoV-2 in sewers,especially in community sewers,is still *** study investigated the virus occurrence by viral nucleic acid testing in vent stacks,septic tanks,and the main sewer outlets of community where confirmed patients had lived during the early days of the epidemic in Wuhan,*** results indicated that the risk of long-term emission of SARS-CoV-2 to the environment via vent stacks of buildings was low after confirmed patients were ***-CoV-2 were mainly detected in the liquid phase,as opposed to being detected in aerosols,and its RNA in the sewage of septic tanks could be detected for only four days after confirmed patients were *** surveillance of SARS-CoV-2 in sewage could be a sensitive indicator for the possible presence of asymptomatic patients in the community,though the viral concentration could be diluted more than ten times,depending on the sampling site,as indicated by the Escherichia coli *** comprehensive investigation of the community sewage drainage system is helpful to understand the occurrence characteristics of SARS-CoV-2 in sewage after excretion with feces and the feasibility of sewage surveillance for COVID-19 pandemic monitoring.

关键词： SARS-CoV-2 Transmission risk Drainage system Sewage Community

Exploration of IRES Elements within the ORF of the Coxsackievirus B3 Genome

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Biomedical and environmental Sciences 2022年第4期35卷 322-333页

作者： SONG Qin Qin LUO Xiao Nuan SHI Bing Tian LIU Mi SONG Juan XIA Dong XIA Zhi Qiang WANG Wen Jun YAO Hai Lan HAN Jun State key laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Molecular Immunology laboratory Capital Institute of PediatricsBeijing 100020China

Objective This study aimed to identify internal ribosome entry sites(IRESs)in the open reading frame(ORF)of the Coxsackievirus B3(CVB3)*** The sequences of P1,P2,or P3 of the CVB3 genome or the truncated sequences from each antithymocyte globulin(ATG)to the end of the P1,P2,or P3 gene were inserted into the *** transfection,possible IRES-dependent green fluorescent protein(GFP)-fused proteins were detected by anti-GFP western *** sequences of possible IRESs were inserted into specific Fluc/Rluc bicistronic vectors,in which the potential IRESs were determined according to the Fluc/Rluc activity *** of Fluc and Rluc mRNA of the bicistronic vector was detected by *** After transfection of full length or truncated sequences of the P1,P2,or P3 plasmids,six GFPfused protein bands in P1,six bands in P2 and nine bands in P3 were detected through western *** IRESs in VP2(1461–1646 nt)and VP1(2784–2983 nt)of P1;one IRES in 2C(4119–4564 nt)of P2;and two IRESs in 3C(5634–5834 nt)and 3D(6870–7087 nt)of P3 were identified according to Fluc/Rluc activity *** cryptic promoter was also excluded by *** Five IRESs are present in the CVB3 coding region.

关键词： CVB3 ORF P1 P2 P3 IRESs