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An innovative classification system for ranking the biological effects of marine aromatic hydrocarbons based on fish embryotoxicity

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Acta Oceanologica Sinica 2024年第6期43卷 153-162页

作者： Ronghui Zheng Chao Fang Fukun Hong Min Zhang Fulong Gao Yusheng Zhang Jun Bo Key Laboratory of Marine Ecological Conservation and Restoration Third Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China Observation and Research Station of Coastal Wetland Ecosystem in Beibu Gulf Ministry of Natural ResourcesBeihai 536015China

Petroleum hydrocarbon pollution is a global concern,particularly in coastal *** aromatic hydrocarbons(PAHs) are regarded as the most toxic components of petroleum *** this study,the biomonitoring and ranking effects of petroleum hydrocarbons and PAHs on the marine fish model Oryzias melastigma embryos were determined in the Jiulong River Estuary(JRE) and its adjacent waters in *** results showed that the levels of petroleum hydrocarbons from almost all sites met the primary standard for marine seawater quality,and the concentrations of the 16 priority PAHs in the surface seawater were lower compared with those in other coastal areas worldwide.A new fish expert system based on the embryotoxicity of ***(OME-FES) was developed and applied in the field to evaluate the biological effects of petroleum hydrocarbons and *** selected physiological index and molecular indicators in OME-FES were appropriate biomarkers for indicating the harmful effects of petroleum hydrocarbons and *** outcome of OME-FES revealed that the biological effect levels of the sampling sites ranged from level Ⅰ(no stress) to level Ⅲ(medium stress),which is further corroborated by the findings of nested analysis of variance(ANOVA) *** results suggest that the OME-FES is an effective tool for evaluating and ranking the biological effects of marine petroleum hydrocarbons and *** method may also be applied to evaluate other marine pollutants based on its framework.

关键词： petroleum hydrocarbons polycyclic aromatic hydrocarbons fish expert system integrated biomarker response nested one-way analysis of variance

Limit Test and pH Determination of 5-Hydroxymethylfurfural in Jiulongteng Honey

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Medicinal Plant 2024年第3期15卷 23-25,29页

作者： Beiqiao YIN Yanyan CHEN Lili LIU Shenggao YIN Yanxia CEN Shiman CHEN Baise People's Hospital Baise 533000China Wuzhou Vocational College Wuzhou 543003China Guangxi University of Chinese Medicine Nanning 530000China beihai Public Inspection and Testing Center Beihai 536000China

[Objectives]To explore the effect of storage time on pH and 5-hydroxymethylfurfural content in Jiulongteng honey.[Methods]The pH of Jiulongteng honey was determined by neutralization titration with sodium hydroxide standard *** content of 5-hydroxymethylfurfural in Jiulongteng honey was determined by *** conditions:ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm)from Agilent Co.,Ltd.,acetonitrile-0.1%formic acid solution(5:95)as mobile phase,flow rate of 0.8 mL/min,5-hydroxymethylfurfural detection wavelength of 284 nm,guanosine detection wavelength of 254 nm.[Results]The pH of 12 batches of Jiulongteng honey was 3.70-3.84 in the new honey stage,3.92-4.05 in the old honey stage 1,and 4.25-4.53 in the old honey stage 2;5-hydroxymethylfurfural was not detected in the new honey stage,5-hydroxymethylfurfural was detected in FM-001 in the old honey stage 1,and 5-hydroxymethylfurfural was detected in most samples in the old honey stage 2.[Conclusions]The pH and 5-hydroxymethylfurfural content of 12 batches of Jiulongteng honey met the requirements within 3 years of *** was no 5-hydroxymethylfurfural in Jiulongteng honey,but with the extension of storage time,the detection amount of 5-hydroxymethylfurfural increased significantly even if Jiulongteng honey was stored at low ***,5-hydroxymethylfurfural can be used as an important indicator of honey freshness.

关键词： Jiulongteng honey 5-hydroxymethylfurfural pH HPLC

Effect of Fe_(2)O_(3)on the Structure,Physical Properties and Crystallization of CaO-Al_(2)O_(3)-SiO_(2)Glass

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Journal of Wuhan University of Technology(Materials Science) 2024年第4期39卷 954-961页

作者：张峰 XIONG Dehua 谢俊张继红 HAN Jianjun CHEN Dequan WEN Zhongquan FAN Zhenhua CHEN Lina SUN Tengfei State Key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China Hepu Research Center for Silicate Materials Industry Technology Beihai 536100China Hainan Institute of Wuhan University of Technology Sanya 430079China Shenzhen Research Institute of Wuhan University of Technology Shenzhen 518000China Chongqing Polycomp Inter-national Corporation Chongqing 400400China

The calcium aluminosilicate-based glasses(CaO-Al_(2)O_(3)-SiO_(2),CAS)with different Fe_(2)O_(3)content(0.10wt%,0.50wt%,0.90wt%,and 1.30wt%)were prepared by traditional melt-quenching *** glass network structure,thermal and mechanical properties,and crystallization behavior changes were investigated by nuclear magnetic resonance spectrometer,Fourier-transform infrared spectro-photometer,X-ray diffractometer,differential scanning calorimetry and field emission scanning electron microscope *** change of Q^(n)in glass structures reveals the glass network connectivity decreases due to the increasing content of Fe_(2)O_(3)addition,resulting in the increasing of non-bridging number in glass *** glass densities slightly rise from 2.644 to 2.681 g/cm^(3),while Vickers’s hardness increases at first,from 6.469 to 6.901 GPa,then slightly drops to 6.745 GPa,with Fe_(2)O_(3)content *** is almost no thermal expansion coefficient change from different Fe_(2)O_(3)*** glass transmittance in visible range gradually decreases with higher Fe_(2)O_(3)content,resulting from the strong absorption of Fe^(2+)and Fe^(3+)*** calculated activation energy from thermal analysis results first decreases from 282.70 to 231.18 kJ/mol,and then increases to 244.02 kJ/mol,with the Fe_(2)O_(3)content increasing from 0.10wt%to 1.30wt%.Meanwhile,the maximum Avrami constant of 2.33 means the CAS glasses exhibit two-dimensional *** of the CAS glass-ceramics samples contain main crystal phase of anorthite,the microstructure appears lamellar and columnar crystals.

关键词： calcium aluminosilicate glass network structure physical properties crystallization

The Fabrication and Detection Performance of High Sensitivity Au-Ag Alloy Nanostar/Paper Flexible Surface Enhanced Raman Spectroscopy Sensors

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Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 436-443页

作者：邓芷盈 WANG Tianyi CAO Shiyi ZHAO Yuan HAN Xiaoyu 张继红谢俊 State Key Laboratory of Silicate Materials for Architectures Department of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China Hainan Institute of Wuhan University of Technology Sanya 572000China International School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China Hepu Research Center for Silicate Materials Industry Technology Beihai 536100China

Au-Ag alloy nanostars based flexible paper surface enhanced Raman spectroscopy sensors were fabricated through simple nanostar coating on regular office paper,and the surface enhanced Raman spectroscopy detection performances were investigated using crystal violet dye ***-Ag nanostars with sharp tips were synthesized via metal ions reduction *** electron microscope images,X-Ray diffraction pattern and energy dispersive spectroscopy elemental mapping confirmed the nanostar geometry and Au/Ag components of the ***-Vis-NIR absorption spectrum shows wide local surface plasmon resonance induced optical *** addition,finite-difference time-domain simulation shows much stronger electromagnetic field from nanostars than from sphere *** effect of coating layer on Raman signal intensities was discussed,and optimized 5-layer coating with best Raman signal was *** Au-Ag nanostatrs homogeneously distribute on paper fiber *** detection limit is 10-10 M,and the relationship between analyte concentrations and Raman signal intensities shows well linear,for potential quantitative *** calculated enhancement factor is 4.795×10^(6).The flexible paper surface enhanced Raman spectroscopy sensors could be applied for trace chemical and biology molecule detection.

关键词： surface-enhanced raman gold-silver alloy nanostars paper-based SERS sensor flexibility

Intervention effect of Cigu Xiaozhi prescription(慈菇消脂方)on ceramide lipoapoptosis in non-alcoholic fatty liver disease

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Journal of Traditional Chinese Medicine 2024年第1期44卷 63-69页

作者： YANG Shaojun MA Yanhua BAI Zhouxia YU Ye FANG Buwu ZHANG Li WANG Li Department of Spleen and Stomach Diseases Beihai Traditional Chinese Medicine hospitalBeihai 536000China the First Clinical Medical College of Gansu University of Traditional Chinese Medicine Lanzhou 730000China Department of Clinical Laboratory Affiliated Hospital of Gansu University of Traditional Chinese MedicineLanzhou 730000China Department of Pharmacology School of Basic MedicineTianjin Medical UniversityTianjin 300070China

OBJECTIVE:To explore the mechanism of the Chinese medicine Cigu Xiaozhi prescription(慈菇消脂方,CGXZ)in the treatment of the non-alcoholic fatty liver disease(NAFLD)by detoxification and phlegm-reducing,the effect of CGXZ prescription on ceramide-mediated lipid apoptosis in Hep G2 cells with ***:The experiment was randomly divided into 6groups:normal control group,model group,CGXZ prescription medicated serum high,medium,and low dose groups,and pioglitazone positive control *** 500μmol/L free fatty acid(FFA)mixture to induce Hep G2 cells to establish NAFLD cell model,respectively,with 2%,4%,and 6%concentration of CGXZ prescription medicated serum intervention for 24 *** changes in organelles and lipid droplet accumulation were observed under the electron ***,Td T-mediated d UTP Nick-End Labeling method was used to assay hepatocyte apoptosis;Biochemical determination of glutamic-pyruvic transaminase,glutamic oxalacetic transaminase,triglycerides,and FFA levels in Hep G2cells;the content of ceramide was determined by highperformance thin-layer ***,Western Blot and quantitative real-time polymerase chain reaction(q RT-PCR)were used to determine the protein and gene expression levels,such as inducible nitric oxide synthase(i NOS),nuclear factorκB(NF-κB),B cell lymphoma 2(Bcl-2)and Bcl-2-associated X(Bax).RESULTS:Under the electron microscope,the cells in the model group showed moderate-to-severe steatosis,and apoptotic bodies could be *** model group had greater improvements in the apoptosis rate(P0.05).Compared with the model group,the levels of ceramide C2 and FFA(P<0.01),the protein expressions of NF-κB,i NOS,and Bax(P<0.05)in the CGXZ prescription treatment group and pioglitazone pos

关键词： non-alcoholic fatty liver disease ceramides Cigu Xiaozhi prescription detoxification and phlegm removal

Effects of Collocational Congruency,Frequency and L2 Proficiency on the Processing of English Adjective-Noun Collocations

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Chinese Journal of Applied Linguistics 2024年第3期47卷 393-413,524页

作者： Lianrui YANG Qun ZHANG Ni LI Ying CHEN Ocean University of China Weifang High Tech Zone beihai School Wuhan University of Science and Technology

The present study investigates the effects of congruency and frequency on adjective-noun collocational processing for Chinese learners of English at two proficiency levels based on the data obtained in an online acceptability judgment *** subject pool of this research included 60 English majors studying at a university in China;30 were selected as a higher-proficiency group and 30 as a lower-proficiency group according to their Vocabulary Levels Test(Schmitt et al.,2001)scores and their self-reported proficiency in *** experimental materials were programmed to E-prime 2.0 and included six types of collocations:(1)15 high-frequency congruent collocations,(2)15 low-frequency congruent collocations,(3)15 high-frequency incongruent collocations,(4)15 low-frequency incongruent collocations,(5)15 Chinese-only items,and(6)75 unrelated items for baseline *** collected response times(RTs)and accuracy rates data were statistically analyzed by the use of an ANOVA test and pairwise comparisons through SPSS 16.0 *** results revealed that:(1)the adjective-noun collocational processing of Chinese English learners is influenced by collocational frequency,congruency and L2 proficiency;(2)the processing time is affected by the interaction of congruency and frequency;and(3)the interactive effect of L2 proficiency in conjunction with congruency and frequency also influences the processing quality.

关键词： adjective-noun collocations congruency effects frequency effects L2 proficiency

Correlation between δ^(18)O, Sr/Ca and Mg/Ca of coral Acropora and seawater temperature from coral culture experiments

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Science China Earth Sciences 2014年第5期57卷 1048-1060页

作者： XIAO YingKai LIU WeiGuo MA YunQi ZHANG YanLing HE MaoYong LUO ChongGuang LIAO QingQiang Qinghai Institute of Salt Lakes Chinese Academy of Sciences State Key Laboratory of Loess and Quaternary Geology Institute of Earth Environment Chinese Academy of Sciences beihai aquarium

To be used as proxies of seawater surface temperature （SST）, the 61Soc values and Sr/Ca and Mg/Ca ratios of scleractinian coral skeletons must be verified by coral culture experiments in the laboratory. This paper describes a coral culture experiment that was conducted at several seawater temperatures T （21-28℃） using a tandem aquarium system and the new method for depositing coral skeletons grown under controlled conditions. The δ180c values and the St/Ca and Mg/Ca ratios of the cultured coral were measured. We concluded that the δ18Oc values and Sr/Ca and Mg/Ca ratios of the cultured coral are clearly corre- lated with T. The linear regression curve is δ18Oc（‰）=-0.1427×T（℃）-0.1495 （n=18, r=0.955, p〈0.0001）, and the slope of -0.1427‰/°d℃ is at the low end of the range of published values （-0.13-0.29‰/°d℃）. The Sr/Ca ratio decreases with increas- ing T, whereas the Mg/Ca ratio increases with increasing T, indicating a negative correlation between Sr/Ca and Mg/Ca. Their linear regression curves are Sr/Ca（mmol/mol）=-O.O4156×T＋lO.59 （n=15, r=-0.789, p〈0.005） and Mg/Ca （mmol/mol）= 0.04974×T＋2.339 （n=17, r=-0.457, p〈0.05）, respectively, which demonstrate that when Mg/Ca and Sr/Ca are increased by one unit, T increases by 5.19℃and decreases by 15.62℃, respectively. These variations are significantly lower than published values.

关键词： coral culture seawater temperature oxygen isotope microelement ratio

Polyp-Canal Reconstruction Reveals Evolution Toward Complexity in Corals

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Research 2024年第1期2023卷 417-428页

作者： Yixin Li Xin Liao Xin Wang Yuanchao Li Hongwei Zhao Yunpeng Zhao Junyuan Chen Chunpeng He Zuhong Lu State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China State Key Laboratory of Coastal and Offshore Engineering Dalian University of TechnologyDalian 116024China Guangxi Key Lab of Mangrove Conservation and Utilization Guangxi Mangrove Research CenterGuangxi Academy of SciencesBeihai 536000China Hainan Academy of Ocean and Fishery Sciences Haikou 571126China State Key Laboratory of Marine Resources Utilization in South China Sea Hainan UniversityHaikou 570228China Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences39 East Beijing RoadNanjing 210008China

Modern scleractinian corals are classified into robust,complex,and basal clades through comparative molecular ***,only few morphological or biological criteria can systematically determine the evolutionary trajectories of these major scleractinian coral ***,we obtained the structural information of 21 scleractinian coral species representing robust and complex clades:High-resolution micro-computed tomography was used to reconstruct the polyp-canal systems in their colonies and to visualize the dynamic polyp growth *** found that the emergence of mesh-like canals may distinguish representatives of complex and robust *** differences in polyp-canal connections suggest distinct evolutionary trajectories among coral species:The formation of the canal network promoted the development of more complex coral structures,and coral polyps within this network formed calices of very similar volume,following precise axial growth *** influence of individual polyps on the coral colony becomes less significant as coral structures become more complex,and coral species with more complicated polyp-canal systems occupied niches more *** work supplements current evolutionary studies on reef-building corals,providing insight for further studies on coral growth patterns.

关键词： Coral representing directions

The land-sourced pollution in the Jiaozhou Bay

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Chinese Journal of Oceanology and Limnology 2008年第2期26卷 229-232页

作者：高振会杨东方秦洁相利宏张柯 beihai Monitoring Center State Oceanic Administration

In recent years, natural environment of the Jiaozhou Bay has been changed largely by fast developing industry and agriculture of the cities around, from which wastewaters were generated. The size of the bay has been continuously shrunk with reduced river flows, resulting in serious contamination to the marine lives in the bay. After analyzing the basic historical data, the authors put forward a suggestion of how to protect the bay ecology for sustaining the resources in the Jiaozhou Bay.

关键词： nutrients nitrogen and phosphorus land-sourced pollution red tide Jiaozhou Bay