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Candidate genes for male and female reproductive traits in Canchim beef cattle

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Journal of Animal Science and Biotechnology 2018年第1期9卷 59-68页

作者： Marcos Eli Buzanskas Daniela do Amaral Grossi Ricardo Vieira Ventura Flavio Schramm Schenkel Tatiane Cristina Seleguim Chud Nedenia Bonvino Stafuzza Luciana Diniz Rola Sarah Laguna Conceio Meirelles Fabiana Barichello Mokry Maurício de Alvarenga Mudadu Roberto Hiroshi Higa Marcos Vinícius Gualberto Barbosa da Silva Maurício Mello de Alencar Luciana Correia de Almeida Regitano Danísio Prado Munari Departamento de Zootecnia Universidade Federal da Paraíba(UFPB)AreiaParailba 58397-000Brazil Fast Genetics SaskatoonSK S7K 2K6Canada beef Improvement Opportunities(BIO) GuelphON N1K 1ESCanada Department of Animal and Poultry Science University of GuelphCentre for Genetic Improvement of Livestock(CGIL)GuelphON N1G 2W1Canada Departamento de Ciencias Exatas Faculdade de Ci6ncias Agrarias e VeterinariasUniversidade Estadual Paulista(Unesp)JaboticabalSao Paulo 14884 900Brazil Departamento de Zootecnia Nucleo de Pesquisa e Conservacao de CervMeosFaculdade de Ciencias Agrarias e VeterinariasUniversidade Estadual Paulista(Unesp)JaboticabalSao Paulo 14884 900Brazil Department of Animal Science Federal University/of Lavras(UFLA)LavrasMinas Gerais 37200 000Brazil Department of Genetics and Evolution Federa University of Sao Carlos(UFSCar)Sao CarlosSao Paulo 13565-905Brazil Embrapa Agricultural Informatics Campinas55o Paulo 13083 886Brazil Embrapa Dairy cattle Juiz de ForaMinas Gerais 36038 330Brazil Embrapa Southeast Livestock Sao CarlosSao Paulo 13560 970Brazil

Background： beef cattle breeding programs in Brazil have placed greater emphasis on the genomic study of reproductive traits of males and females due to their economic importance. In this study, genome-wide associations were assessed for scrotal circumference at 210 d of age, scrotal circumference at 420 d of age, age at first calving, and age at second calving, in Canchim beef cattle. Data quality control was conducted resulting in 672,778 SNPs and 392 ***： Associated SNPs were observed for scrotal circumference at 420 d of age（435 SNPs）, followed by scrotal circumference at 210 d of age（12 SNPs）, age at first calving（six SNPs）, and age at second calving（four SNPs）. We investigated whether significant SNPs were within genic or surrounding regions. Biological processes of genes were associated with immune system, multicellular organismal process, response to stimulus, apoptotic process, cellular component organization or biogenesis, biological adhesion, and ***： Few associations were observed for scrotal circumference at 210 d of age, age at first calving, and age at second calving, reinforcing their polygenic inheritance and the complexity of understanding the genetic architecture of reproductive traits. Finding many associations for scrotal circumference at 420 d of age in various regions of the Canchim genome also reveals the difficulty of targeting specific candidate genes that could act on fertility; nonetheless,the high linkage disequilibrium between loci herein estimated could aid to overcome this issue. Therefore, al relevant information about genomic regions influencing reproductive traits may contribute to target candidate genes for further investigation of causal mutations and aid in future genomic studies in Canchim cattle to improve the breeding program.

关键词： Animal breeding Composite breed Genome-wide association Genomic data Single nucleotide polymorphism

The community structure and microbial linkage of rumen protozoa and methanogens in response to the addition of tea seed saponins in the diet of beef cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 272-281页

作者： Cui Tan Carlos A.Ramírez-Restrepo Ali Mujtaba Shah Rui Hu Matt Bell Zhisheng Wang Chris McSweeney Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu 611130SichuanChina Commonwealth Scientific and Industrial Research Organisation CSIRO Agriculture and FoodAustralian Tropical Sciences and Innovation PrecinctJames Cook UniversityTownsville QLD 4811Australia Present address:CR Eco-efficient Agriculture Consultancy(CREAC) 46 Bilbao PlaceBushland Beach QLD 4818Australia Department of Livestock Production Shaheed Benazir Bhutto University of Veterinary and Animal SciencesSakrandSindh 67210Pakistan “Low Carbon Breeding cattle and Safety Production” University Key Laboratory of Sichuan ProvinceYa’an 625014SichuanChina School of Biosciences University of NottinghamSutton BoningtonLoughborough LE125RDUK CSIRO Agriculture Queensland BioScience PrecinctSt LuciaBrisbane QLD 4067Australia.

Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 ***: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.

关键词： beef steers Methane Methanogen Protozoa Tea seed saponin

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饲料在肉牛饲养领域的加工与利用

中国畜牧杂志 2015年第S1期51卷 44-45页

作者： Bartle S J Simroth JC Schwandt E F 胡玉梅 beef cattle Institute Kansas State University

谷物作为肉牛饲料由于诸多原因必须经过加工处理,但是最主要的原因还是由于加工可以提高谷物的消化率。谷物颗粒的最外层的作用是为了保护谷物的繁殖器官和储存在谷物里的能量来源(胚乳或者淀粉)不被环境和生物破坏。但是,为了有效地利... 详细信息

谷物作为肉牛饲料由于诸多原因必须经过加工处理,但是最主要的原因还是由于加工可以提高谷物的消化率。谷物颗粒的最外层的作用是为了保护谷物的繁殖器官和储存在谷物里的能量来源(胚乳或者淀粉)不被环境和生物破坏。但是,为了有效地利用谷物,这些起保护作用的外层必须被破坏。

关键词：肉牛饲养牛饲料繁殖器官高水分玉米消化率 starch 能量比干物质采食量锤式粉碎机 moisture

Effects of different dietary energy and protein levels and sex on growth performance,carcass characteristics and meat quality of F1 Angus x Chinese Xiangxi yellow cattle

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Journal of Animal Science and Biotechnology 2014年第4期5卷 485-496页

作者： Lingyan Li Yuankui Zhu Xianyou Wang Yang He Binghai Cao National beef cattle Industry and Technology System College of Animal Science and TechnologyChina Agricultural University Tin Wah Industrial Co.Ltd Shimen Industrial Area of Lian Yuan

Background：The experiment evaluated the effect of nutrition levels and sex on the growth performance,carcass characteristics and meat quality of F1 Angus × Chinese Xiangxi yellow ***：During the background period of 184 d,23 steers and 24 heifers were fed the same ration,then put into a2×2×2 factorial arrangement under two levels of- dietary energy（TON：70/80%DM）,protein（CP：11.9/14.3%DM）and sex（S：male/female） during the finishing phase of 146 *** treatments were-（1） high energy/low protein（HELP）,（2） high energy/high protein（HEHP）,（3） low energy/low protein（LELP） and（4） low energy/high protein（LEHP）.Each treatment used 6 steers and 6 heifers,except for HELP- 5 steers and 6 ***：Growth rate and final carcass weight were unaffected by dietary energy and protein levels or by *** with the LE diet group,the HE group had significantly lower dry matter intake（DMI,6.76 vs.7.48 kg DM/d）,greater chest girth increments（46.1 vs.36.8 cm）,higher carcass fat（19.9 vs.16.3%） and intramuscular fat content（29.9 vs.22.8%DM）.The HE group also had improved yields of top and medium top grade commercial meat cuts（39.9 vs.36.5%）.The dressing percentage was higher for the HP group than the LP group（53.4 vs.54.9%）.Steers had a greater length increment（9.0 vs.8.3 cm）,but lower carcass fat content（16.8 vs.19.4%） than *** meat quality traits（shear force value,drip loss,cooking loss and water holding capacity） were not affected by treatments or sex,averaging 3.14 kg,2.5,31.5 and 52.9%,*** nutritive profiles（both fatty and amino acid composition） were not influenced by the energy or protein levels or by ***：The dietary energy and protein levels and sex significantly influenced the carcass characteristics and chemical composition of meat but not thegrowth performance,meat quality traits and nutritive profiles.

关键词： Carcass characteristics Energy F1 Angus × Chinese Xiangxi yellow cattle Growth performance Meat quality Protein Sex

Identification of key genes and metabolites involved in meat quality performance in Qinchuan cattle by WGCNA

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Journal of Integrative Agriculture 2024年第11期23卷 3923-3937页

作者： Hengwei Yu Zhimei Yang Jianfang Wang Huaxuan Li Xuefeng Li Entang Liang Chugang Mei Linsen Zan College of Animal Science and Technology Northwest A&F UniversityYangling 712100China College of Grassland Agriculture Northwest A&F UniversityYangling 712100China National beef cattle Improvement Center Yangling 712100China

Understanding the genetic and metabolic elements that impact meat quality is crucial to improving production and meeting consumer demands in the beef *** in meat quality among various muscle areas in beef cattle can impact pricing in the *** progress in genomics,the specific genes and metabolites that affect meat quality characteristics in Qinchuan cattle remain inadequately ***,this study aims to evaluate the meat quality characteristics of four specific muscle locations(tenderloin,striploin,high rib,and ribeye muscles)in Qinchuan bulls,including 10 traits(total protein content(TPC),intramuscular fat(IMF),non-esterified fatty acid(NEFA),meat color(L*,a*,and b*),shear force(SF),cooking loss(CL),pH0,and pH24).This experiment uses transcriptome,metabolome sequencing,and sophisticated analytical methodologies such as weighted gene co-expression network analysis(WGCNA)and protein–protein interaction networks(PPI)to identify the key genes and metabolites associated with specific *** findings highlight three notable genes(NDUFAB1,NDUFA12,and NDUFB7)linked to intramuscular fat(IMF),three key genes(CSRP3,ACAA3,and ACADVL)correlated with non-esterified fatty acids(NEFA),and one crucial gene(CREBBP)influencing meat *** conclusion,this investigation offers a new perspective on the differences in bovine muscle locations and contributes to the molecular understanding of bovine meat *** research endeavors could delve deeper into the identified genes and pathways to enhance beef cattle’s quality and yield.

关键词： meat quality performance WGCNA cattle transcriptome metabolome

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肉牛围栏育肥场常见疾病的有效预防

中国畜牧杂志 2015年第S1期51卷 147-148页

作者： Dave Rethors 吴鑫 beef cattle Institute Kansas State University

2011年国家动物健康监督体系(NAHMS)出版的《围栏育肥场研究》报道,美国围栏育肥场有16.2%的牛会出现牛呼吸道疾病(BRD),这个概率超过了其他5类疾病发生率的总和:消化不良(4.3%)、非典型间质性肺炎(2.8%)、配种失败(2.8%)、跛行(1.8%)... 详细信息

2011年国家动物健康监督体系(NAHMS)出版的《围栏育肥场研究》报道,美国围栏育肥场有16.2%的牛会出现牛呼吸道疾病(BRD),这个概率超过了其他5类疾病发生率的总和:消化不良(4.3%)、非典型间质性肺炎(2.8%)、配种失败(2.8%)、跛行(1.8%)和中枢神经系统疾病(1.1%)。这意味着。

关键词：育肥场间质性肺炎 weaning cattle 呼吸道疾病疾病发生率断奶应激 digestive 应激源抗生素耐药性

Effects of Fleckvieh on the Performance of Dual-Purpose cattle Improved by Hybridization with Local beef Cows

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Animal Husbandry and Feed Science 2015年第6期7卷 322-326,357页

作者： Wang Linfeng Yang Gaiqing Lian Hongxia Yan Ping Liu Xian Liu Yan Wang Wenhui Zhu Ruiguang Li Ming Yang Guoyu College of Animal Science and Veterinary Medicine Henan Agricultural University Modern Experimental Technique and Managing Center Henan Agricultural University Shangqiu Animal Disease Prevention and Control Center Henan General Station of Animal Husbandry College of Henan Agricultural Vocation Henan Breeding cattle Genetic Performance Test Center Kaifeng Animal Husbandry Work Station

[Objective] The paper aimed to study the improved effect of local beef cattle with Fleckvieh cattle and provide theoretical basic data for local cattle industry in central plain agricultural area in China. [Method] With 500 local beef cattle as female parents and Fleckvieh cattle as male parents,hybridization improvement was conducted via artificial insemination. The growth performance,slaughter performance,milk performance and milk components of F1 and F2 hybrids were measured. [Result] The birth body weights of F2 were significantly higher than those of local beef cow,but there was no remarkable difference between F1 and local beef cow or F1 and F2. The growth rates of F1 and F2 at different stages were higher than those of local beef cattle. The slaughter performance,such as carcass weight( P beef cow. Milk production performance,such as actual milk yield,305 d corrected milk yield and 4% standard milk yield of F2 were signally higher than those of F1 and local beef cattle( Pbeef cattle( P beef cattle at varying degrees,they were still at high levels compared with Holstein cows.[Conclusion]Fleckvieh cattle,as a male parent,can significantly improved growth performance,slaughter performance and milk performance of offsprings. It would also increases the economic efficiency of local beef cattle by higher quality and price,as well as changing production model from beef to dual purpose of beef and milk.

关键词： Dual-purpose cattle Hybridization and improvement Growth performance Production performance Milk quality

MED4 gene positively affects preadipocyte differentiation in Chinese red steppe cattle

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BIOCELL 2023年第9期47卷 2115-2123页

作者： MINGHONG WEI CHENG XIAO JIAN WU LIHONG QIN HONGLIANG LIU YANG CAO YUMIN ZHAO Institute of Animal Biotechnology Jilin Academy of Agricultural SciencesGongzhuling136100China Institute of Muscle Biology and Growth Research Institute for Farm Animal Biology(FBN)Dummerstorf18196Germany Key Laboratory of beef cattle Genetics and Breeding Ministry of Agriculture and Rural AffairsGongzhuling136100China

Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality *** Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,livestock growth,carcass traits,and triglyceride ***,the physiological function of the MED4 gene on bovine adipocyte differentiation remains ***:This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe *** overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western ***:The MED4 gene had significantly high expression during preadipocyte differentiation(pcattle by regulating the PPARγgene.

关键词： Chinese red steppe cattle MED4 PPARγ Predipocyte differentiation Lipid metabolism Meat quality trait

Active dry yeast supplementation improves the growth performance,rumen fermentation, and immune response of weaned beef calves

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Animal Nutrition 2021年第4期7卷 1352-1359页

作者： Jian Ma Cheng Wang Zhisheng Wang Guang Cao Rui Hu Xueying Wang Huawei Zou Kun Kang Quanhui Peng Bai Xue Lizhi Wang Yueming Zhu Xiaopeng Zhu Low Carbon Breeding cattle and Safety Production University Key Laboratory of Sichuan Province Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130China Angel Yeast Co. Ltd.Yichang 443000China Zhangye Comprehensive Experimental Station of National Yak and beef Industrial Technology System Zhangye 734000China

The objective of this experiment was to investigate the potential benefits of active dry yeast(ADY)on the growth performance,rumen fermentation,nutrient digestibility,and serum parameters of weaned beef *** Simmental crossbred male calves(body weight=86.47±4.41 kg and 70±4 d of age)were randomly divided into 2 groups:control(CON)(fed basal ration)and ADY(fed basal ration and 5 g/d ADY per calf).The dietary concentrate-to-roughage ratio was 35:*** the calves were regularly provided rations 3 times a day at 07:00,13:00,and 19:00 and had free access to wate *** experiment lasted for 60 *** average daily gain of ADY group was higher(P=0.007)than that of the CON group,and the ratio of feed intake to average daily gain in the ADY group was reduced(P=0.022)as compared to the CON *** concentration of ruminal ammonia-N was higher(P=0.023)in the CON group than that in the ADY group,but an opposite trend of microbial protein was found between the 2 ***,the ruminal concentrations of propionate and butyrate were higher(Pbeef calves.

关键词： Active dry yeast Weaned beef calf Growth performance Rumen fermentation Nutrient digestibility Immune response