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Journal of Biosafety and Biosecurity 2024年第1期6卷 16-26页

作者： Alina CTeuscher Charlotte Ruhnau Nicole Stöcklin Fabienne Wichmann Evelyn Ilg Hampe Claudia Bagutti State Laboratory Basel-City Biosafety LaboratoryBaselSwitzerland Novatis Lichtstrasse 354056 BaselSwitzerland1 IQVIA ag Kirschgartenstrasse 144051 BaselSwitzerland1 becton dickinson ag Binningerstrasse 944123 AllschwilSwitzerland1

Laboratory wastewater has been suggested as an important escape route for microorganisms from research *** reasons for the unintentional release of laboratory organisms are shortcomings in the handling of infectious liquid biological waste(LBW)and inadequate inactivation *** developed an analytical approach to investigate the use of chemical inactivation(CI)procedures in Swiss research laboratories by on-site random sampling of presumably inactivated infectious LBW and testing it for the presence of infectious lentiviruses(HIV-1)and adenoviruses(AdV).In addition,standard operating procedures(SOPs)for CI were collected and evaluated,and laboratorystaff knowledge of CI processes was assessed using a *** we found several deficiencies in the technical knowledge and training of laboratory staff on the CI of LBW,as documented by 27 returned questionnaires,no infectious viruses were detected in the eight LBW samples *** we acknowledge that the number of LBW samples and SOPs is small,we conclude that CI of LBW containing infectious lentiviruses and adenoviruses does not result in the systematic release of considerable amounts of infectious viruses into the environment from research laboratories in Switzerland.

关键词： Chemical inactivation Infectious liquid biological waste Lentivirus(HIV-1) Adenovirus

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流式细胞术

微生物与感染 2008年第2期3卷 125-126页

作者：曾令武 becton-dickinson公司

流式细胞术发展简史流式细胞术(flow cytometry,FCM)是一种可以对细胞或亚细胞结构进行快速测量的新型分析技术和分选技术.其特点是:①测量速度快,最快可在1s内计测数万个细胞;②可进行多参数测量,对同一个细胞做有关物理、化学特性的... 详细信息

流式细胞术发展简史流式细胞术(flow cytometry,FCM)是一种可以对细胞或亚细胞结构进行快速测量的新型分析技术和分选技术.其特点是:①测量速度快,最快可在1s内计测数万个细胞;②可进行多参数测量,对同一个细胞做有关物理、化学特性的多参数测量,并具有明显的统计学意义;③是一门综合性的高科技方法,它综合了激光技术、计算机技术、流体力学、细胞化学、图像技术等众多领域的知识和成果;④既是细胞分析技术,又是精确的分选技术.

关键词：流式细胞术分选技术多参数测量计算机技术发展简史快速测量细胞结构测量速度

应用流式细胞分析仪同时分析细胞表面抗原及DNA含量

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上海医学 1991年第5期14卷 253-256页

作者：唐惟瑜许福熙 L.Van Hove W Boogaerts 上海医科大学附属肿瘤医院 Leuven University Hospital Belgium becton dickinson Immunocytometry Systems

作者在应用流式细胞分析仪用于分析 DNA 和细胞表面标记的基础上,进一步发展成同时分析 DNA 和细胞表面抗原。其法是首先将细胞用结合荧光的抗体染色,在免疫染色后,再用0.5%多聚甲醛100μl 将细胞固定5分钟,然后以碘化丙啶(Propidium io... 详细信息

作者在应用流式细胞分析仪用于分析 DNA 和细胞表面标记的基础上,进一步发展成同时分析 DNA 和细胞表面抗原。其法是首先将细胞用结合荧光的抗体染色,在免疫染色后,再用0.5%多聚甲醛100μl 将细胞固定5分钟,然后以碘化丙啶(Propidium iodide,PI)染色,避光保存。用0.5%多聚甲醛固定能很好的保存细胞表面结合韵抗体,经 DNA 分析,变异系数(CV)平均为4%(2.1%～6.3%)。在细胞表面抗原呈现的基础上可鉴别细胞的类型,同时通过细胞表面抗原与细胞周期相结合的方法进行分析。

关键词：细胞分析仪流式表面抗原

Antituberculosis and cytotoxic activities of triorganotin(Ⅳ) complexes

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Chinese Chemical Letters 2012年第6期23卷 731-735页

作者： Hidayat Hussain NaziaBibi Ahmed Al-Harrasi Salman Siddiqi Shahana U. Kazmi Ying Zhang Amin Badshah Department of Chemisty University of Paderborn 33098 Paderborn Germany Department of Biological Siences and Chemistry College of Arts and Sciences University of Nizwa Oman Immnology and Infectious Disease Research Laboratory Department of Microbiology University of＂ Karachi Pakistan Department of Molecular Muclvbiology and Immunology Bloomberg School of Public Health Johns Hopkins University Baltimore MD 21205 USA becton dickinson Diagnostic Systems Sparks MD 21152 USA Department of Chemistry Quaid-e-Azam University lslamabad 45320 Pakistan

Five triorganotin（IV）（1-5） complexes of cinnamic acid, （Z）-2-acetamido-3-phenylacrylic acid, 3-methylbut-2-enoic acid, and 2,2-diphenylacetic acid have been synthesized and characterized by IH-13C-119Sn NMR, UV, and IR. The spectroscopic investigation demonstrated that the carboxylate group acts as a monodentate ligand in triorganotin（IV） compounds. Five triorganotin（IV） complexes were screened against the log phase culture of Mycobacterium tuberculosis H37Rv by colorimetric method using XTT dye as growth indicator. The MICs were found to he 0.08 and 1.25μg/mL.

关键词： Triorganotin(IV) complexes Antituberculosis activity MICs Cytotoxicity

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领带引发的收购风波

IT时代周刊 2014年第24期24卷 52-53页

作者：弗里克.韦穆伦阿什拉夫.祖阿曼伦敦商学院 becton dickinson & Co北美区

我们通过清晰可见的行云力来表达看不见的价值观和信条,而它们共同构成了身份。一些看似微不足道的细节,能带来举足轻重的后果大多数高管都意识到并购的任务艰巨。学术研究显示,60%至80%的并购以失败告终。大多数高管也强调并购后的整... 详细信息

我们通过清晰可见的行云力来表达看不见的价值观和信条,而它们共同构成了身份。一些看似微不足道的细节,能带来举足轻重的后果大多数高管都意识到并购的任务艰巨。学术研究显示,60%至80%的并购以失败告终。大多数高管也强调并购后的整合和文化冲击问题很大,常常剑拔弩张,给企业带来长期和巨大的破坏。遗憾的是,这些认识虽然广为人知,但并购还是普遍无法创造价值,依然会造成巨大的问题。

关键词：风波收购引发领带学术研究冲击问题创造价值并购

Early use of dexamethasone increases Nr4a1 in Kupffer cells ameliorating acute liver failure in mice in a glucocorticoid receptor-dependent manner

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第9期21卷 727-739页

作者： Jing-wen DENG Qin YANG Xiao-peng CAI Jia-ming ZHOU Wei-gao E Yan-dong AN Qiu-xian ZHENG Meng HONG Yan-li REN Jun GUAN Gang WANG Shu-jing LAI Zhi CHEN State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseasesthe First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003China Center for Stem Cell and Regenerative Medicine Zhejiang University School of MedicineHangzhou 310058China becton Dickinson and CompanyShanghai 200126China

Background and objective:Acute liver failure(ALF)is a type of disease with high mortality and rapid progression with no specific treatment methods currently *** exert beneficial clinical effects on therapy for ***,the mechanism of this effect remains unclear and when to use glucocorticoids in patients with ALF is difficult to *** purpose of this study was to investigate the specific immunological mechanism of dexamethasone(Dex)on treatment of ALF induced by lipopolysaccharide(LPS)/D-galactosamine(D-Ga IN)in ***:Male C57 BL/6 mice were given LPS and D-Ga IN by intraperitoneal injection to establish an animal model of *** was administrated to these mice and its therapeutic effect was *** and eosin(H&E)staining was used to determine liver *** flow cytometry,cytometric bead array(CBA)method,and next-generation sequencing were performed to detect changes of messenger RNA(m RNA)in immune cells,cytokines,and Kupffer cells,***:A mouse model of ALF can be constructed successfully using LPS/D-Ga IN,which causes a cytokine storm in early disease *** immune cells change markedly with progression of liver *** use of Dex,at 0 h rather than 1 h,could significantly improve the progression of ALF induced by LPS/D-Ga IN in *** of innate immune cells,especially Kupffer cells and neutrophils,increased significantly in the Dex-treated *** vivo experiments indicated that the therapeutic effect of Dex is exerted mainly via the glucocorticoid receptor(Gr).Sequencing of Kupffer cells revealed that Dex could increase m RNA transcription level of nuclear receptor subfamily 4 group A member 1(Nr4 a1),and that this effect disappeared after Gr ***:In LPS/D-Ga IN-induced ALF mice,early administration of Dex improved ALF by increasing the numbers of innate immune cells,especially Kupffer cells and ***-dependent Nr4 a1 upregul

关键词： Glucocorticoid Dexamethasone Kupffer cells Acute liver failure Nuclear receptor subfamily 4 group A member 1(Nr4a1)

Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis

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Chinese Medical Journal 2019年第13期132卷 1572-1581页

作者： Dong-Bo Chen Yang-Jing Zhao Xue-Yan Wang Wei-Jia Liao Pu Chen Kang-Jian Deng Xu Cong Ran Fei Xu Wu Qi-Xiang Shao Lai Wei Xing-Wang Xie Hong-Song Chen Peking University People's Hospital Peki叩University Hepatology InstituteBeijing Key Laboratory of Hepatitis C and Immunotherapy for Liver DiseaseBeiji叩100044China Department of Immunology The Key Laboratory of Laboratory Medicine of Jiangsu ProvinceSchool of MedicineJiangsu UniversityZhenjiangJiangsu 212013China Laboratory of Hepatobiliary and Pancreatic Surgery Affiliated Hospital of Guilin Medical UniversityGuilinGuangxi 541001China Center of Excellence Becton Dickinson BiosciencesBeijing 100176China

Background:Our previous studies have shown that regulatory factor X5(RFX5),a classical transcription regulator of MHCⅡ genes,was obviously overexpressed in hepatocellular carcinoma(HCC)***,the role of RFX5 in the carcinogenesis and progress of HCC remains *** study aimed to reveal its biological significance and the underlying mechanism in ***:RFX5 mRNA expression level and copy number variation in HCC tumors and cell lines were determined by analyzing deposited data sets in the Cancer Genome Atlas and Gene Expression Omnibus *** biological significance of RFX5 in HCC was investigated by monitoring the colony formation and subcutaneous tumor growth capacity when RFX5 was silenced with lentiviral short hairpin RNA and CRISPR7Cas9 system in HCC cell *** downstream gene transcriptionally activated by RFX5 in HCC cells was determined by chromatin immunoprecipitation and luciferase reporter *** involvement of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta(YWHAQ)in HCC development was further determined by performing colony formation rescue assay and subcutaneous tumor growth rescue *** association of YWHAQ with recurrence-free survival of patients with HCC was assessed by Kaplan-Meier ***,apoptosis level and the protein level of p53 pathway were determined to reveal the mechanism of RFX5 in driving HCC ***:RFX5 was amplified and highly overexpressed in HCC tumor tissues compared with the corresponding non-tumor *** mRNA expression level of RFX5 was significantly correlated with its DNA copy number(r=0.4,P<0.001).Functional study demonstrated that RFX5 was required for both clonogenic forming in vitro and subcutaneous tumor growth in vivo of HCC *** study identified YWHAQ,namely 14-3-3 tau,as a key downstream transcriptional target gene of RFX5,which was tightly regulated by RFX5 in ***,overexpression of YWHAQ largely rescued t

关键词： Hepatocellular carcinoma Transcription factor Apoptosis P53

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用基因转染技术生产一种新的单克隆抗体

细胞与分子免疫学杂志 1987年第1期 100-100页

作者： Sherie L.Morrison.,杨成勇 Department of Microbiolgy. Columbia University College of physicians and Surgeons 701 west 168th Street New York New York 10032 and becton-dickinson Monoclonal Center 2375 Garcia Avenue Mountain View California 94043

一种将免疫球蛋白(Ig)基因传染到淋巴细胞中的方法业已建立。传染到基因得到如实表达,并在传染链和内源链及两个传染链间发生组合。可用基因传染来重建Ig分子,并产生新的免疫球蛋白分子。这些新的分子能代表重链与轻链间专一结合,换言之... 详细信息

一种将免疫球蛋白(Ig)基因传染到淋巴细胞中的方法业已建立。传染到基因得到如实表达,并在传染链和内源链及两个传染链间发生组合。可用基因传染来重建Ig分子,并产生新的免疫球蛋白分子。这些新的分子能代表重链与轻链间专一结合,换言之,用DNA重组技术可在体外组合,转染及表达基因。这样,就能在种内及种间进行有效的转换。作者用此

关键词：基因抗原

对老年性黄斑变性患者佩戴棱镜后矫正作用进行判定的随机对照试验

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世界核心医学期刊文摘（眼科学分册） 2005年第12期1卷 23-24页

作者： Smith H.J dickinson C.M Cacho I 王文军 Arthritis Research Centre Robert Jones and Agnes Hunt Orthopaedic Hospital Gobowen Nr Oswestry SY10 7AG United Kingdom.Dr

Objective: To determine the effectiveness of prism spectacles in people with age-related macular degeneration by relocating the retinal image. Methods: We implemented a randomized, placebo-controlled, double-masked trial. Participantswith agerelated macular degeneration received a standard low-vision assessment and the prescription of conventional low-vision aids 6 weeks before the study intervention. Participants were randomized to receive 1 of the following, including the optimal refractive correction: (1) custom, incorporating bilateral prisms to match participants’ preferred power and base direction; (2) standard, incorporating standard bilateral prisms (6 prism diopters Δ base up for logMAR [logarithm of the minimum angle of resolution]visual acuity (VA) of 0.48- 1.00 and 10Δ base up for logMAR VA of 1.02- 1.68); or (3) placebo, consisting of spectacles matched in weight and thickness to prism spectacles but without prism. Main Outcome Measures: Outcomes measured binocularly at baseline and 3- month follow-up included distance logMAR VA, reading speed, critical print size, visual functioning questionnaires, and observed visual task performance. Scores on the 25- item National Eye Institute Visual Functioning Questionnaire and the Melbourne Low-Vision ADL (Activities of Daily Living) Index were converted to linear estimates usingRasch analysis. The Manchester Low Vision Questionnaire was used to collect descriptive data. Results: A total of 225 participants completed the trial (median age, 81 years). We found no significant effect of treatment group on any of the outcome measures, includingVA, the primary outcome (adjusted for baseline) (P=.63). Participants’ responses to the Manchester Low Vision Questionnaire suggested that the prism spectacles added to their problems. Conclusions: Prism spectacles are no more effective than conventional spectacles for peoplewith age-relatedmacular degeneration.

关键词：老年性黄斑变性随机对照试验三棱镜屈光度低视力眼镜处方视功能矫正效果屈光矫正安慰剂对照双盲试验