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Fast fluorescence lifetime imaging techniques:A review on challenge and development

Journal of Innovative Optical Health Sciences 2019年第5期12卷 3-29页

作者： Xiongbo Liu Danying Lin Wolfgang becker Jingjing Niu Bin Yu Liwei Liu Junle Qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060 P.R.China becker&hickl gmbh nunsdorfer Ring 7-9 Berlin 12277Germany

Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early ***,there is a large interest in obtaining FLIM data within an acquisition time as short as ***,there is currently a technology that advances towards faster and faster FLIM ***,the maximum speed of a recording technique is only part of the *** acquisition time of a FLIM image is a complex function of many *** include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological *** from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.

关键词： Fluorescence lifetime imaging microscopy(FLIM) acquisitin time imaging speed dead time photon fficiency time domain frequency domain scanning wide-field imaging time-correlated single photon counting(TCSPC) gated detection gated image intensifer modulated inage intensifier SPAD array detector

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A pilot study of the dynamics of tissue oxygenation in vivo using time-resolved phosphorescence imaging

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Journal of Innovative Optical Health Sciences 2021年第5期4卷 15-24页

作者： Natalia Kazachkina Julia Lymar Vladislav Shcheslavskiy Alexander Savitsky A.N.Bach Institute of Biochemistry Research Center of Biotechnology of the Russian Academy of Sciences Moscow 119071Russian Federation Institute of Experimental Oncology and Biomedical Technologies Privolzhsky Research Medical University Sq Minin and Pozharsky10/1603005 Nizhny Novgorod Russian Federation becker and hickl gmbh nunsdorfer Ring 7-912277 BerlinGermany

Oxygenation of tissues plays an important role in the development and progression of tumor to treatment *** of metalloporphyrines phosphorescence quenching by oxygen is one of the ways to measure dynamics of the oxygen concentration in the tissues by phosphorescence lifetime imaging of meso-tetra(sulfophenyl)tetrabenzoporphyrin Pd(Ⅱ)(TBP)using the time-correlated single photon counting(TCSPC)*** has been shown that phosphorescence lifetime of the sensor in S37 tumor in vivo varied in the range of 130 to 290μs after both topical and intravenous administration of *** indicates that oxygen level in tumors was lower compared to normal tissues where TBP phosphorescence has not been *** lifetimes of TBP increased in the solid tumor and in the muscle after photodynamic therapy of solid tumor that demonstrates oxygen consumption during treatment and possibly stopping the blood flow and hence the oxygen supply to the tissues.

关键词： Phosphorescence oxygen sensor metalloporphyrines TCSPC

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Fast repetition rate fs pulsed lasers for advanced PLIM microscopy

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Journal of Innovative Optical Health Sciences 2019年第5期12卷 86-94页

作者： Sviatlana Kalinina Alexander Jelzow Tobias Plotzing Angelika Rück University of Ulm Core Facility Confocal and Multiphoton MicroscopyN24 Albert-Einstein-Allee 1189081 UlmGermany becker&hickl gmbh Nahmitzer Damm 3012277 BerlinGermany Laser Quantum gmbh Max-Stromeyer-Str.11678467 KonstanzGermany

Simultaneous metabolic and oxygen imaging is promising to follow up therapy response,dis-ease development and to determine prognostic *** of metabolic coenzymes is now widely accepted to be the most reliable method to determine cellular ***,oxygen consumption has to be taken into account to understand treatment *** phosphorescence lifetimne of oxygen sensors is able to indicate local oxygen *** phosphorescence lifetime imaging(PLIM)dyes based on ruthenium(I)coordination com-plexes are useful,in detaill TLD1433 which possesses a variety of different triplet states,enables complex photochemistry and redox *** is usally reached by two photon exci-tation of the drug with a femtosecond(fs)pulsed Ti:Sapphire laser working at 80 MHz repe-tition rate and(time-correlated single photon counting)(TCSPC)detection *** interesting question was whether it is possible to follow up PLIM 1using faster repetition *** repetition rates could be advantageous for the induction of specific photochemical reactions because of similar light doses used normally in standard CW light *** this,a default 2p-FLIM-PLIM system was expanded by adding a second fs pulsed laser("helixx")which provides 50 fs pulses at a repetition rate of 250 MHz,more than 2.3 w average power and tunable from 720 nm to 920 *** laser beam was coupled into the AOM instead of the default 80 MHz *** demonstrated siuccessful applications of the 250 MHz laser for PLIM which correlates well with measurements done by excitation with the conventional 80MHx laser source.

关键词： Multiphoton microscopy axygen consumption phosphorescence lifetime microscopy fast repetition rate excitation short laser pulses Ru(I)complexes

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