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Involvement of blood mononuclear cells in the infertility, age-associated diseases and cancer treatment

World Journal of Stem Cells 2016年第12期8卷 399-427页

作者： Antonin Bukovsky Laboratory of Reproductive Biology biocev Institute of Biotechnology Czech Academy of Sciences

Blood mononuclear cells consist of T cells and monocyte derived cells. Beside immunity, the blood mononuclear cells belong to the complex tissue control system(TCS), where they exhibit morphostatic function by stimulatingproliferation of tissue stem cells followed by cellular differentiation, that is stopped after attaining the proper functional stage, which differs among various tissue types. Therefore, the term immune and morphostatic system(IMS) should be implied. The TCS-mediated morphostasis also consists of vascular pericytes controlled by autonomic innervation, which is regulating the quantity of distinct tissues in vivo. Lack of proper differentiation of tissue cells by TCS causes either tissue underdevelopment, e.g., muscular dystrophy, or degenerative functional failures, e.g., type 1 diabetes and age-associated diseases. With the gradual IMS regression after 35 years of age the gonadal infertility develops, followed by a growing incidence of age-associated diseases and cancers. Without restoring an altered TCS function in a degenerative disease, the implantation of tissue-specific stem cells alone by regenerative medicine can not be successful. Transfused young blood could temporarily restore fertility to enable parenthood. The young blood could also temporarily alleviate aging diseases, and this can be extended by substances inducing IMS regeneration, like the honey bee propolis. The local and/or systemic use of honey bee propolis stopped hair and teeth loss, regressed varicose veins, improved altered hearing, and lowered high blood pressure and sugar levels. Complete regression of stage Ⅳ ovarian cancer with liver metastases after a simple elaborated immunotherapy is also reported.

关键词： Blood mononuclear cells Age-associated diseases Infertility treatment Regenerative medicine Transfusion morphostatic treatment Stem cells Tissue control system Immune system Tissue morphostasis Cancer immunotherapy

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Distinct gut microbiota profiles in patients with primary sclerosing cholangitis and ulcerative colitis

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World Journal of Gastroenterology 2017年第25期23卷 4548-4558页

作者： Lukas Bajer Miloslav Kverka Martin Kostovcik Peter Macinga Jiri Dvorak Zuzana Stehlikova Jan Brezina Pavel Wohl Julius Spicak Pavel Drastich Department of Gastroenterology and Hepatology Institute for Clinical and Experimental Medicine Institute of Microbiology the Czech Academy of Sciences Institute of Experimental Medicine the Czech Academy of Sciences Department of Genetics and Microbiology Faculty of ScienceCharles University biocev Institute of Microbiologythe Czech Academy of Sciences

AIM To characterize the gut bacterial microbiota of patients with primary sclerosing cholangitis(PSC) and ulcerative colitis(UC).METHODS Stool samples were collected and relevant clinical data obtained from 106 study participants, 43 PSC patients with(n = 32) or without(n = 11) concomitant inflammatory bowel disease, 32 UC patients, and 31 healthy controls. The V3 and V4 regions of the 16 S ribosomal RNA gene were sequenced on Illumina Mi Seq platform to cover low taxonomic levels. Data were further processed in QIIME employing Ma As Lin and LEf Se tools for analysis of the output data.RESULTS Microbial profiles in both PSC and UC were characterized by low bacterial diversity and significant change in global microbial composition. Rothia, Enterococcus, Streptococcus, Veillonella, and three other genera were markedly overrepresented in PSC regardless of concomitant inflammatory bowel disease(IBD). Rothia, Veillonella and Streptococcus were tracked to the species level to identify Rothia mucilaginosa, Streptococcus infantus, S. alactolyticus, and S. equi along with Veillonella parvula and V. dispar. PSC was further characterized by decreased abundance of Adlercreutzia equolifaciens and Prevotella copri. Decrease in genus Phascolarctobacterium was linked to presence of colonic inflammation regardless of IBD phenotype. Akkermansia muciniphila, Butyricicoccus pullicaecorum and Clostridium colinum were decreased in UC along with genus Roseburia. Low levels of serum albumin were significantly correlated with enrichment of order Actinomycetales.CONCLUSION PSC is associated with specific gut microbes independently of concomitant IBD and several bacterial taxa clearly distinguish IBD phenotypes(PSC-IBD and UC).

关键词： Dysbiosis 煽动性的肠疾病 Ulcerative 大肠炎内脏 microbiota 主要 sclerosing 胆管炎

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仅在永久性脑缺血后短暂出现的星形胶质细胞样NG2胶质细胞亚群

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神经损伤与功能重建 2021年第8期16卷 F0003-F0003页

作者： Denisa Kirdajova Lukas Valihrach Martin Valny Jan Kriska Daniela Krocianova Sarka Benesova Pavel Abaffy Daniel Zucha Ruslan Klassen Denisa Kolenicova Pavel Honsa Mikael Kubista Miroslava Anderova 杜一星(编译) Department of Cellular Neurophysiology Institute of Experimental MedicineCzech Academy of SciencesPragueCzech Republic Second Faculty of Medicine Charles UniversityCzech Republic Laboratory of Gene Expression Institute of Biotechnology CASBIOCEVVestecCzech Republic Faculty of Chemical Technology Laboratory of Informatics and ChemistryUniversity of Chemistry and TechnologyPragueCzech Republic Faculty of Science Charles UniversityPragueCzech Republic 不详

NG2神经胶质细胞在生理和病理条件下显示出较强的增殖和分化潜力。本研究用3月龄Cspg4启动子下由tdTomato标记NG2神经胶质细胞的小鼠,在不同类型的脑疾病模型(局灶性脑缺血、皮质刺伤和脱髓鞘)中研究NG2细胞的增殖和分化潜力,研究首先... 详细信息

NG2神经胶质细胞在生理和病理条件下显示出较强的增殖和分化潜力。本研究用3月龄Cspg4启动子下由tdTomato标记NG2神经胶质细胞的小鼠,在不同类型的脑疾病模型(局灶性脑缺血、皮质刺伤和脱髓鞘)中研究NG2细胞的增殖和分化潜力,研究首先采用单细胞RT-qPCR和Kohonen自组织映射图分析从未损伤和特定损伤后的皮质/胼胝体分离的tdTomato阳性细胞,比较不同损伤后NG2神经胶质细胞表达概况。这种方法使我们能够区分NG2神经胶质细胞和少突胶质细胞,每个细胞群都包含4个不同的亚群。基因表达谱显示,表达GFAP(反应性星形胶质细胞的标志物)的NG2胶质细胞亚群仅在局灶性脑缺血后短暂出现。然而,在较轻的损伤后(皮质刺伤和脱髓鞘),反映少突胶质细胞成熟不同阶段的亚群明显占优势。免疫组织化学也证实了不同亚群的损伤依赖性发生率。本研究使用单细胞RNA测序分析这种独特的短暂星形胶质细胞样NG2胶质细胞亚群,并采用膜片钳技术揭示了其基本的细胞膜特性。综上所述,我们证明了星形胶质细胞样NG2神经胶质细胞是仅在局灶性脑缺血后短暂出现的NG2神经胶质细胞的特定亚群。这些位于缺血后神经胶质瘢痕中的细胞处于活跃的细胞周期,并显示出与皮质星形胶质细胞相似的电流模式,可能是缺血后胶质瘢痕形成和修复过程中的重要参与者。

关键词：星形胶质细胞脱髓鞘缺血 NG2胶质细胞少突胶质细胞刺伤

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